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At least 37 records · Page 2Linked to original sources
PubMed2005

Two-beam optical traps: refractive index and size measurements of microscale objects.

A counter-propagating optical trap measurement (COTM) system is proposed and analyzed based on the ray-optics model. In this system, refractive index and size of trapped objects can be estimated by using forward scattered light from the two-beam laser trap with resolution Delta n = 0.013 for the refractive index measurements and 3.3% for the size measurements, which is comparable with current bulk techniques, such as refractometry, and flow cytometry. The unique advantage of the COTM system over conventional approaches lies in its capability of marker-free single-particle characterization in whatever transparent buffer required by living cell, eliminating the necessity of changing the fluid composition of the sample in refractometry, and of tagging target with toxic fluorescence dyes in flow cytometry. Noise analysis predicts a potential improvement in the system resolution by more than two orders of magnitude. This non-invasive and sterile tool complements lab-on-a-chips with single cell manipulation and analysis in living friendly ambient.

Biopolymers↗
PubMed2005

Negative index of refraction in optical metamaterials.

A double-periodic array of pairs of parallel gold nanorods is shown to have a negative refractive index in the optical range. Such behavior results from the plasmon resonance in the pairs of nanorods for both the electric and the magnetic components of light. The refractive index is retrieved from direct phase and amplitude measurements for transmission and reflection, which are all in excellent agreement with simulations. Both experiments and simulations demonstrate that a negative refractive index n' approximately -0.3 is achieved at the optical communication wavelength of 1.5 microm using the array of nanorods. The retrieved refractive index critically depends on the phase of the transmitted wave, which emphasizes the importance of phase measurements in finding n'.

Journal Article↗
PubMed2006

Very low-refractive-index optical thin films consisting of an array of SiO2 nanorods.

The refractive-index contrast in dielectric multilayer structures, optical resonators, and photonic crystals is an important figure of merit that creates a strong demand for high-quality thin films with a low refractive index. A SiO2 nanorod layer with low refractive index of n = 1.08, to our knowledge the lowest ever reported in thin-film materials, is grown by oblique-angle electron-beam deposition of SiO2. A single-pair distributed Bragg reflector employing a SiO2 nanorod layer is demonstrated to have enhanced reflectivity, showing the great potential of low-refractive-index films for applications in photonic structures and devices.

Journal Article↗
PubMed2001

Electromagnetic wave chaos in gradient refractive index optical cavities.

Electromagnetic wave chaos is investigated using two-dimensional optical cavities formed in a cylindrical gradient refractive index lens with reflective surfaces. When the planar ends of the lens are cut at an angle to its axis, the geometrical ray paths are chaotic. In this regime, the electromagnetic mode spectrum of the cavity is modulated by both real and ghost periodic ray paths, which also "scar" the electric field intensity distributions of many modes. When the cavity is coupled to waveguides, the eigenmodes generate complex series of resonant peaks in the electromagnetic transmission spectrum.

Journal Article↗
PubMedDate not supplied

Refractive surgery, optical aberrations, and visual performance.

Visual optics is taking on new clinical significance. Given that current refractive procedures can and do induce large amounts of higher order ocular aberration that often affects the patient's daily visual function and quality of life, we can no longer relegate the considerations of ocular aberrations to academic discussions. Instead, we need to move toward minimizing (not increasing) the eye's aberrations at the same time we are correcting the eye's spherical and cylindrical refractive error. These are exciting times in refractive surgery, which need to be tempered by the fact that after all the research, clinical, and marketing dust settles, the level to which we improve the quality of the retinal image will be guided by the trade-off between cost and the improvement in the quality of life that refractive surgery offers.

Cornea↗
PubMed2005

Focusing hard x rays to nanometer dimensions by adiabatically focusing lenses.

We address the question of what is the smallest spot size that hard x rays can be focused to using refractive optics. A thick refractive x-ray lens is considered, whose aperture is gradually (adiabatically) adapted to the size of the beam as it converges to the focus. These adiabatically focusing lenses are shown to have a relatively large numerical aperture, focusing hard x rays down to a lateral size of 2 nm (FWHM), well below the theoretical limit for focusing with waveguides [C. Bergemann, Phys. Rev. Lett.912003204801].

Journal Article↗
PubMed1991

Effect of refraction on optical microscopic measurement of internal blood-vessel diameter and its correction.

In optical microscopic measurement of internal blood-vessel diameters, the effect of refraction must be taken into account to ensure accuracy of the result. This effect is discussed and an analytical correction formula derived. Phantom blood vessels with known internal and external diameters were used to test the validity of the correction formula. The errors obtained prior to correction were reduced significantly after correction.

Blood Vessels↗
PubMed2001

Computerized image analysis of Ki-67 in ductal breast carcinoma in situ.

OBJECTIVE: To develop and determine the staining protocols and computerized image analysis methods that are the most effective combination for performing quantitative analysis of Ki-67. STUDY DESIGN: We compared conventional bright-field light microscopy and refractive optical enhancement methods in combination with various immunodetection and filter enhancement methods, including immunogold in combination with epipolarization refractive optics and enzymatic conversion of chromogenic substrates in combination with optical filter enhancement. Initial Ki-67 tests were performed on lymph node tissues and cultured human breast cells and then applied to 200 ductal carcinoma in situ (DCIS) samples. DCIS acini were digitally acquired, and a region of interest was manually outlined in each one with a digital stylus to include only the cellular component; then the Ki-67 staining index was quantified by segmentation analysis. RESULTS: Although combining epipolarization analysis with immunohistogold staining was the most sensitive detection method, nonspecific binding was too high. The streptavidin-horseradish-peroxidase enzymatic conversion of 3,3'-diaminobenzidine (DAB) in combination with optical enhancement filters was the most effective method tested. Ki-67 stain was associated with dense fibrillar structures of the nucleoli in the less intensely staining nuclei and was most intense in paired nuclei. CONCLUSION: The method of measuring Ki-67 expression by DAB staining combined with optical enhancement filters and quantification via computer-assisted image analysis techniques produced objective and reproducible results. As such, this method can offer (1) less intraobserver and interobserver variability, (2) a digital archival record, and (3) a baseline for digital exchange of information between studies.

Antibodies, Monoclonal↗
PubMed2005

Long-term follow-up of ultrathin corneas after surface retreatment with phototherapeutic keratectomy.

PURPOSE: To evaluate visual acuity and long-term stability after phototherapeutic keratectomy (PTK) in patients with corneal thickness less than 400 microm and cornea-related vision problems. SETTING: Department of Ophthalmology, Istituto Clinico Humanitas, Rozzano-Milan, Italy. METHODS: This retrospective study comprised 48 eyes that had PTK for complications after photorefractive keratectomy that removed more than 200 microm of tissue. Phototherapeutic keratectomy was performed with the Nidek EC-5000 excimer laser with 10 Hz frequency, intraoperative topography, and masking fluid over an ablation diameter of 10.0 mm. The follow-up was 5 years. The mean preoperative best spectacle-corrected visual acuity (BSCVA) was 0.2 +/- 0.09 (SD) with -2.53 +/- 2.34 diopters (D), and the mean pachymetry was 390 +/- 38 microm. The efficacy parameters were pachymetry and visual acuity at 1 year and the stability parameters, optical refraction, corneal curvature (calculated as the mean curvature over the entire 3.0 mm and 5.0 mm central zones), and pachymetry from 1 to 5 years. RESULTS: At 1 year, the mean BSCVA was 0.6 +/- 0.72 with -2.15 +/- 1.67 D and the mean pachymetry, 341 +/- 40 microm. At 5 years, the mean BSCVA was 0.7 +/- 0.15 with -2.33 +/- 1.12 D and the mean pachymetry, 339 +/- 48 microm. In all patients, there was an improvement of 4 or more Snellen lines. Statistical evaluation of refraction and corneal curvature values at 1 and 5 years indicated no statistically significant differences. CONCLUSIONS: After PTK in eyes with ultrathin corneas, there was significant improvement in BSCVA and long-term stability of the optical refraction, corneal curvature, and pachymetry. In selected cases, 10.0 mm ablation zone PTK may be an alternative to penetrating keratoplasty, offering long-term corneal stability.

Adult↗
PubMed2002

Design and analysis of an integrated antiresonant reflecting optical waveguide refractive-index sensor.

An integrated optical waveguide refractometer, believed to be novel, is presented. The sensor is based on an antiresonant reflecting optical waveguide and uses the strong attenuation dependence on the refractive index of antiresonant cladding layers as the sensing principle. The theory and the operation of the sensor are discussed in terms of one- and two-dimensional geometry. The theoretical predictions and numerical analysis show that a versatile sensor can be realized. The design trade-offs are discussed, and the sensitivity and measurement range are presented.

Journal Article↗
PubMed2005

Optical transillumination tomography with tolerance against refraction mismatch.

Optical transillumination tomography (OT) is a laser-based imaging modality where ballistic photons are used for projection generation. Image reconstruction is therefore similar to X-ray computed tomography. This modality promises fast image acquisition, good resolution and contrast, and inexpensive instrumentation for imaging of weakly scattering objects, such as for example tissue-engineered constructs. In spite of its advantages, OT is not widely used. One reason is its sensitivity towards changes in material refractive index along the light path. Beam refraction artefacts cause areas of overestimated tissue density and blur geometric details. A spatial filter, introduced into the beam path to eliminate scattered photons, will also remove refracted photons from the projections. In the projections, zones affected by refraction can be detected by thresholding. By using algebraic reconstruction techniques (ART) in conjunction with suitable interpolation algorithms, reconstruction artefacts can be partly avoided. Reconstructions from a test image were performed. Standard filtered backprojection (FBP) showed a round mean square (RMS) deviation from the original image of 9.9. RMS deviation with refraction-tolerant ART reconstruction was 0.33 and 0.24, depending on the algorithm, compared to 0.57 (FBP) and 0.06 (ART) in a non-refracting case. In addition, modified ART reconstruction allowed detection of small geometric details that were invisible in standard reconstructions. Refraction-tolerant ART may be the key to eliminating one of the major challenges of OT.

Algorithms↗