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PubMed1977

HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA.

The DNAs of two closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNAs showed identical restriction patterns in an internal region comprising approximately 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 X 10(6) daltons, some 6 X 10(6) daltons less than VV DNA. The two opposite terminal restriction fragments of RPV DNA cross-hybridized to each other.

Base Sequence↗

PubMed1975

Preliminary studies on an unusual poxvirus of the western grey squirrel (Sciurus griseus griseus) of North America.

Particles of a poxvirus found in Californian western grey squirrels have overall dimensions indistinguishable from those of the poxviruses of the genera Orthopoxvirus and Leporipoxvirus, but have surface structures somewhat reminiscent of virions of members of the genus Parapoxvirus. Extracts of tissues infected with the squirrel poxvirus cross-react in gel-diffusion tests with extracts of tissues infected with Californian myxoma virus, but not with a similar preparation infected with a South American strain of myxoma virus.

Animals↗

PubMed1978

Pox infection in white rats.

2 pox outbreaks among white rats in a breeding colony are described. The infection occurred in 3 different forms: pulmonary, dermal and mixed. Apparently healthy animals appeared to be virus carriers. The virus isolated belonged to the genus Orthopoxvirus of Poxviridae family, and was very close to cowpox virus. It differed from reference strains of cowpox virus in having a lower ceiling temperature and a higher pathogenicity for white rats.

Animals↗

PubMed1978

Comparative studies on poxvirus strains isolated from camels.

Five poxvirus strains isolated from camels in Egypt, Iran and the U.S.S.R. were investigated. The morphology and size of the negatively stained virions of the investigated strains were identical. The strains produced minute white pock lesions localized mainly on the infected area of the chorioallantoic membrane, without generalization of lesions or death of embryos. The ceiling temperature and the haemagglutinating activity of the different strains were different. All the strains were apathogenic for rabbit skin and highly pathogenic for mice inoculated intracerebrally. Serologically, all the strains were related to vaccinia virus but with different degrees. The studied strains had the characteristics of members of the genus Orthopoxvirus.

Animals↗

PubMed1976

A method for serological differentation of closely related poxviruses.

A two-step gel precipitation reaction with antiserum to vaccinia virus, based on sequential addition of two test viruses was elaborated for differentiation of closely related orthopoxviruses. The appearance of an additional precipitation line indicated differences in the antigenic structure of the viruses compared. Differences in the antigenic structure of vaccinia, variola and monkey pox viruses as well as the antigenic identity with cow pox virus of poxviruses isolated from carnivora, elephants and okapi were established.

Epitopes↗

PubMed2025

A Quantitative Real-Time PCR Assay for Measuring Poxvirus Replication and Cell Binding.

Quantitative real-time PCR (qPCR) is a fast and reliable method to quantify viral genomes as a surrogate to titering on monolayers of cells for measuring virus replication. Whether it be for determining the number of virions released, the total number of genomes produced during infection, or the number of virions bound to a cell, qPCR assays can be adapted to quickly enumerate total viral genomes in a broad range of experiments comparing virus replication under different conditions. In addition, qPCR offers several advantages compared to plaque assays including time, linearity over 9 logs, and scalability from tens-to-hundreds of samples, depending on the qPCR machine. Here we describe a qPCR assay for quantifying vaccinia virus' dsDNA genome that can be used to determine the total number of virions produced. Furthermore, we describe a straightforward protocol for a cell-binding assay that is sensitive enough to use with small concentrations of inoculating virions. This protocol is suitable for measuring the cell-binding ability of mutations that affect virus production and infectivity.

Virus Replication↗