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PubMed1982

Differentiation of coagulase-positive and coagulase-negative staphylococci by lectins and plant agglutinins.

The screening of staphylococci with a panel of 14 lectins and extracts demonstrating lectin-like activity led to the development of a rapid agglutination slide test for the differentiation of certain coagulase-negative staphylococci and human strains of Staphylococcus aureus. The coagulase-negative staphylococci were agglutinated by agglutinins from Mangifera indica, Triticum vulgaris, and crude Limulus polyphemus. The test is rapid, requiring only 5 to 15 min to identify an unknown strain of staphylococci, as opposed to the 4 to 16 h required to perform the conventional tube coagulase test.

Agglutination Tests↗
PubMed1984

The interaction of Brucella cell surface components with plant agglutinins.

Cells of all Brucella species were agglutinated by the basic protein-reactive lectins of Mangifera indica and Persea americana. Cells of non-smooth strains including B. ovis and B. canis but not smooth strains were also agglutinated by concanavalin A. These lectins also reacted in a similar pattern with lipopolysaccharide extracts of these organisms. The lectin from Bandeiraea simplicifolia precipitated with the lipopolysaccharide antigens of smooth Brucella organisms and with those of serologically cross-reactive strains of Escherichia coli, Pseudomonas maltophilia, Salmonella and Yersinia enterocolitica but not with antigenically unrelated strains.

Agglutination Tests↗