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At least 37 records · Page 2Linked to original sources
PubMed1978

RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions.

Rates of formation of RNA:DNA hybrids have been measured as a function of temperature and compared to DNA:RNA duplex denaturation temperatures in 4 M sodium perchlorate, 4 M NaClO4-6 M urea, and 3 M rubidium trichloracetate solvents. The usual bell shaped curves of reaction rate versus temperature were observed. The optimal temperatures for the RNA:DNA association reaction are 5 degrees to 12 degrees greater than the Tm's for DNA:DNA denaturation in these solvents, just as in formamide. R-loops of phi80d3ilv DNA with E. coli rRNA can be formed at high efficiency in these solvents.

DNA, Bacterial↗
PubMed1978

Extraction of skin test activity from Coccidioides immitis mycelia by water, perchloric acid, and aqueous phenol extraction.

Water, perchloric acid extracts, and fractions of partially defatted whole mycelia of Coccidioides immitis contained delayed skin test activity when tested in C. immitis-infected guinea pits. Aqueous phenol extraction of these fractions resulted in partitioning of activity between aqueous-soluble and phenol-soluble fractions; activity was found to be water soluble after removal of phenol by extensive dialysis. Highest specific activity skin test antigen was invariably found in the phenol-soluble phase, water-soluble fraction. Material of equivalent activity could also be extracted directly from the defatted mycelia. Skin test active fractions contained glucose, mannose, 3-O-methylmannose, glucosamine, and amino acids.

Animals↗
PubMed1978

Continuous-flow analysis of ammonia in perchloric acid supernate of blood or plasma using an ammonia-selective electrode.

A simple automated method for the estimation of ammonia in perchloric acid supernate of blood or plasma using an ion-selective electrode (Orion Ammonia-selective electrode, Model 95-10) is described. The reliability of the proposed method has been checked against an ion-exchange resin procedure, which has been chosen as a standard procedure. Regression equation and correlation coefficient for the proposed method are y = 0.7 X + 10 and 0.945, respectively, as compared with the chosen standard method. Within-run and between-run precision are 2.1% and 3.5% respectively. The average percent recovery is 97.5% and a tentative range is 13-73 microgram/dl (9-52 micronmol/l) ammonia nitrogen.

Adult↗
PubMed1975

Perchloric acid-soluble proteins and protein-bound hexoses in serum and tissues of normal, hypo-and hyperthyroid rats.

The total content of perchloric acid-soluble proteins (PCA-SP) and protein-bound hexoses (PBH) were studied in blood serum and tissues (liver, kidney, lung, brain) of normal, hypo- and hyperthyroid rats. The results obtained indicate that the hyperthyroid state induced an increase in both PCA-SP and PBH in blood serum and studied tissue homogenates, and hypothyreosis causes a decrease of total glycoproteins content in all studied materials. The results were counted per 1 g of protein and in this way the nonspecific effect of thyroid hormones on the protein metabolism had no influence on the measurements.

Animals↗
PubMed1978

Buffer-exchange column for rapid separation of carcinoembryonic antigen from perchloric acid.

A Sephadex G-50 medium (Pharmacia) buffer-exchange column has been developed for rapidly changing the medium for carcinoembryonic antigen from perchloric acid to acetate buffer. Analytical recovery of the extracted antigen exceeded 95%. Plasma for analysis can be so prepared and samples ready for analysis within an hour. The technique also results in uniform assay volume, thus eliminating this variable from the assay.

Buffers↗
PubMed1977

Hydrolysis mechanism of 7-acetylacroninium perchlorate, a novel prodrug of acronine.

7-Acetylacroninium perchlorate was hydrolyzed at 25 degrees by both water enriched with 18O-labeled water and by unenriched water. The acronine obtained was examined by mass spectrometry, which indicated the unusual fact that hydrolysis of this ester proceeded by aryl oxygen cleavage to the extent of about 30% under those mild conditions.

Acronine↗
PubMedDate not supplied

[Muscle glycoproteins (soluble in perchloric acid) after denervation and drug stimulation].

Muscle proteins soluble in perchloric acid contain about 75% of carbohydrates presented by neutral hexoses, fucose and hexuronic acids. Three days and two weeks after denervation the content of the glycoproteids protein part is unchanged, a month later it increases by 116%. The content of carbohydrate component following denervation lowers considerably and a month later is on the average 40% higher than in the muscle of healthy rats. Administration of muscular RNA restores the content of the protein component only by the end of a month; administration of pyrogenal normalizes the content of both protein and carbohydrate components.

Animals↗