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Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control.

Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.

Animals

[Pest control and yield increase of areca by root-zone application of carbofuran in admixture with fertilizers].

The result of our experiment has shown that by root-zone application of carbofuran in admixture with fertilizers to Areca in spring the serious pest Tirathaba rufivena can be controlled for 4 months during flowering and young fruiting stage and the yield can increase by 50%. The final residual amount of carbofuran in fruits is below 0.1-0.01 ppm or too low to be examined, and there is no decrease in total alkaloid content in fruits.

Alkaloids

Monitoring Ontario-grown apples for pest control chemicals used in their production, 1978-86.

Between 1978 and 1986, 305 samples of apples were monitored for the residues of a wide range of pesticides used in their production. Three (1%) contained residues above the maximum residue limits (MRL) permitted under the Canadian Food and Drug Act and regulations; two involved phosalone at 5.9 and 6.2 mg/kg respectively and one involved diphenylamine at 6.7 mg/kg when the MRL was 5.0 mg/kg for both compounds. Low residues of dicofol, endosulfan, phosalone, phosmet, captan, daminozide and diphenylamine were frequently found; however they were well below the MRLs. These residue levels were correlated with survey data on the areas of the apple crop treated with specific pesticides. Residues of carbaryl, diazinon, ethion, azinophosmethyl, parathion, and dithiocarbamate fungicides were found occasionally; all were well below the MRLs and correlated with the pattern of use. No residues of PCB were found to a limit of detection of 0.01 mg/kg.

Environmental Monitoring

[Examination of persons in contact with plant protection and pest control agents].

It is referred to the great importance of the annual controls of farmers who work with preventive remedies for plants and insecticides. Alone during a two-year control 4-7 per cent of disabled persons and 15-18 percent who needed by all means a control were found. Among the laboratory values, apart from the quantitative determination of cholesterinase, above all the determination of the transaminases, the thymol test and the G-6-PhD stood the test. These examinations must by all means be carried out already before the beginning of the work. The number of persons who have to be controlled must be enlarged. Publications concerning other results hitherto yielded and the consequences which must be derived from this are desirable.

Agricultural Workers' Diseases

[Epicutaneous testing with plant protective and pest control agents (pesticides)].

A catalogue concerning adequate concentrations of pesticides for use in patch testing is presented. These recommendations are based on author's experiences also utilizing case reports both of the GDR and of the international literature. In particular case reports are mentioned on contact dermatitis due to Metham-sodium, Dazomet, Zineb and Maneb, Benomyl, Captan, Propachlor, Phenmedipham, 2,4-D and 2,4,5-T, Dichlorvos, Parathionmethyl, Trichlorfon. The recommended patch test concentrations should be made a subject for discussion and further investigations.

Agriculture

Pesticides and the Third World.

Many developing countries are importing industrial processes that make use of toxic chemicals. By the same token, pesticides, which are toxic by design, are also used increasingly in agriculture and in public health programs to control pests and vector-borne diseases. Recent estimates suggest that pesticides account for more than 20,000 fatalities yearly, and that most of these will have occurred in developing countries. This may actually be a gross underreporting. Although organophosphate and carbamate insecticides are still responsible for many of those poisoning cases, herbicides such as paraquat are also increasingly being implicated in fatal poisoning cases. Newer pesticides such as the synthetic derivatives of pyrethrin, which were believed to be relatively safe to humans, now appear to be implicated in some serious cases of intoxication. Community-based pest control using locally available botanical pesticides could have severe consequences unless the toxicity of these compounds is carefully assessed relative to nontarget organisms. A high proportion of pesticide intoxications appear to be due to lack of knowledge, unsafe attitudes, and dangerous practices. The technology available to small farmers for pesticide application is often inappropriate: faulty sprayers, lack of protective equipment adapted to tropical conditions, nonexistent first-aid provisions. Agricultural extension is often not oriented to the transfer of information relative to the dangers inherent in the use of pesticides. The lack of information at all levels may be one of the most important causative factors of chemical intoxication in developing countries. Research should at this time concentrate on behaviors leading to chemical intoxication. This should be done concurrently with proper prospective and retrospective surveys of poisonings in developing country communities. More information should be sought relative to the decision processes of import, legislation, and licensing. Research and development efforts in appropriate technology and safety devices are also critically needed.

Animals

Endocrine strategies for the control of ectoparasites and insect pests.

The increasing knowledge about endocrine mechanisms in arthropods facilitates the biorational search for drugs against insect pests and parasites that interfere with arthropod hormone action. Juvenile hormone mimics have been successfully applied for about 20 years; however, resistance to juvenile hormone analogues has developed. The introduction of moulting hormone agonists, which compete for binding to the ecdysteroid receptor, is expected in the near future. Despite the considerable progress that has been achieved in peptide hormone research during the last few years, no successful insecticide is currently available, although comparisons of drugs for medical use demonstrate that in principle, successful interference with peptide hormone action is possible. The search for new drugs has been facilitated by advances in cell-culture techniques, which improve the development of suitable screening systems, and by progress in genetic engineering, which could be an important tool in the creation of new strategies for insect pest control.

Animals