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Results for “Retinal Horizontal Cells”

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At least 37 records · Page 2Linked to original sources

PubMed1996

Reconstruction of retinal horizontal cell responses by the ionic current model.

An ionic current model of the retinal horizontal cell is constructed. The horizontal cell models are interconnected by gap junctions to form a horizontal cell layer. The light response properties of the L-type horizontal cell are analyzed using this model. We demonstrate the functional role of each ionic current and the role of the feedback loop between cones and horizontal cells. The present study provides insight into the dynamic relationships between characteristics on the cellular level and on the multi-cellular level for producing the light response in horizontal cells.

Action Potentials↗

PubMed1996

Dopamine modulates unitary conductance of single PL-type calcium channels in Roccus chrysops retinal horizontal cells.

1. Dopamine modulation of the PL-type calcium channel of white bass retinal horizontal cells was studied in isolated, cultured neurons. Single-channel recordings were made of calcium channels in outside-out patches, under conditions which favoured the expression of calcium channel activity. 2. Analysis of single-channel properties revealed that dopamine potentiated the activity of the sustained calcium channel in three ways. First, it increased unitary conductance through individual channels. Under the influence of dopamine, single-channel conductance doubled. 3. Dopamine also increased the probability of channel opening and increased channel mean open time. The probability of opening increased 4-fold while mean open time doubled. 4. The mean closed time was also affected. The time between individual openings was not affected but the closed time between bursts of openings was shortened by over 50%. 5. The effects of dopamine were mediated via the activation of a D1-type receptor and the resulting activation of a cAMP-mediated second messenger system. 6. The combination of the effects of dopamine significantly increased the net calcium influx into the cell.

Animals↗

PubMed2003

Glutamate modulation of GABA transport in retinal horizontal cells of the skate.

Transport of the amino acid GABA into neurons and glia plays a key role in regulating the effects of GABA in the vertebrate retina. We have examined the modulation of GABA-elicited transport currents of retinal horizontal cells by glutamate, the likely neurotransmitter of vertebrate photoreceptors. Enzymatically isolated external horizontal cells of skate were examined using whole-cell voltage-clamp techniques. GABA (1 mM ) elicited an inward current that was completely suppressed by the GABA transport inhibitors tiagabine (10 microM) and SKF89976-A (100 microM), but was unaffected by 100 microM picrotoxin. Prior application of 100 microM glutamate significantly reduced the GABA-elicited current. Glutamate depressed the GABA dose-response curve without shifting the curve laterally or altering the voltage dependence of the current. The ionotropic glutamate receptor agonists kainate and AMPA also reduced the GABA-elicited current, and the effects of glutamate and kainate were abolished by the ionotropic glutamate receptor antagonist 6-cyano-7-nitroquinoxaline. NMDA neither elicited a current nor modified the GABA-induced current, and metabotropic glutamate analogues were also without effect. Inhibition of the GABA-elicited current by glutamate and kainate was reduced when extracellular calcium was removed and when recording pipettes contained high concentrations of the calcium chelator BAPTA. Caffeine (5 mM) and thapsigargin (2 nM), agents known to alter intracellular calcium levels, also reduced the GABA-elicited current, but increases in calcium induced by depolarization alone did not. Our data suggest that glutamate regulates GABA transport in retinal horizontal cells through a calcium-dependent process, and imply a close physical relationship between calcium-permeable glutamate receptors and GABA transporters in these cells.

Animals↗

PubMed1989

Bifurcation analysis of nonlinear retinal horizontal cell models. I. Properties of isolated cells.

1. Bifurcation theory is used to study properties of nonlinear analytical and computational models of isolated retinal horizontal cells. The analytical model is based on the published data of Shingai and Christensen describing steady-state I-V characteristics of horizontal cells isolated from catfish (Ictalurus punctatus) retina. The computational model is based on I-V characteristics of distinct macroscopic membrane currents observed in horizontal cells isolated from goldfish (Carassius auratus) retina. Slow-model dynamics are analyzed assuming that excitatory processes occur rapidly with respect to the time course of inactivation of the inward Ca2+ and outward K+ currents. 2. A global bifurcation diagram plotting the location and stability properties of critical points as a function of photoreceptor-evoked horizontal-cell postsynaptic membrane conductance Gsyn is derived for the analytical model. The automated bifurcation analysis software AUTO is used to compute global bifurcation diagrams for the computational model. Bifurcation diagrams exhibit a bistable regime at small Gsyn values characterized by two stable and one unstable critical point and a monostable regime at larger Gsyn values characterized by a single globally attracting stable critical point. The transition between bistable and monostable behavior occurs at a Gsyn value of roughly 0.9 nS for the computational model and 1.7 nS for the analytical model. Estimates of horizontal-cell glutamate-channel conductance suggest that this transition corresponds to the activation of as few as 400-700 glutamate channels. Dark-evoked release of neurotransmitter from photoreceptors may therefore set horizontal-cell synaptic conductance Gsyn to a value within the monostable regime. 3. Photoreceptor-evoked horizontal-cell membrane conductance, total Ca2+ channel conductance, and inactivation of the inward Ca2+ current are shown to be the major factors controlling the bifurcation structure of the computational model. Inactivation of the inward Ca2+ current is required to account for the dark resting potential of horizontal cells as well as light-evoked hyperpolarizing responses. Inactivation of the outward K+ current has little effect on model properties. 4. Isolated horizontal cells generate Ca2+ action potentials whereas cells in the intact retina normally do not. Simple procedures for modeling the slow dynamics of isolated horizontal-cell Ca2+ action potentials are described.(ABSTRACT TRUNCATED AT 400 WORDS)

Algorithms↗

PubMed2001

Gating of retinal horizontal cell hemi gap junction channels by voltage, Ca2+, and retinoic acid.

PURPOSE: Hemi gap junction (HGJ) channels, precursors of gap junctional channels, are functionally expressed in retinal horizontal cells where they may play roles in osmoeregulation and ephaptic regulation of synaptic feedback to photoreceptors. In this study we examined mechanisms of gating of these channels by transmembrane voltage, Ca2+ and retinoic acid (RA). METHODS: Experiments were performed on cultured bass horizontal cells using the conventional whole cell patch clamp configuration. RESULTS: HGJ currents in isolated bass horizontal cells, revealed by perfusion with Ca2+ free media, were opened by positive holding potentials and inhibited by negative holding potentials. These currents were also inhibited by external application of either Ca2+ or RA. Using a rapid perfusion system, the latency of 2 mM Ca2+ to begin channel closure was unmeasurably brief, whereas the latency for 30 microM RA action was 177+/-9 ms (mean+/-standard error of the mean). The total inhibition of HGJ channel currents by coapplication of 0.3 microM RA and 100 microM Ca2+ was less than the sum of inhibition by RA alone and Ca2+ alone suggesting that the actions of RA and Ca2+ were not independent. In the presence of 0.3 microM RA, the half maximal concentration for Ca2+ inhibition was increased from a control value of 192 microM to 375 microM without affecting maximal inhibition. Similarly, the half maximal concentration for RA inhibition was increased from a control value of 0.44 microM to 1.1 microM without affecting maximal inhibition in the presence of 100 microM Ca2+. CONCLUSIONS: These results suggest that horizontal cell HGJ channels are closed by the normal negative resting potentials of these cells. Extracellular Ca2+ and the retinal neuromodulator RA also act to close HGJ channels through mechanisms or sites which are not independent.

Action Potentials↗

PubMed1990

Background-induced flicker enhancement in cat retinal horizontal cells. I. Temporal and spectral properties.

1. Dim backgrounds can enhance small-spot flicker responses of cat retinal horizontal cells by a factor of 2 or more. 2. Intracellular marking with horseradish peroxidase (HRP) reveals that this enhancement effect occurs in--but is not necessarily limited to--the cone-connected, A-type horizontal cell. 3. Flicker amplitudes decrease over a frequency range from 3 to 36 Hz of square-wave photic stimulation. There is little evidence of flicker-response enhancement at 3 Hz. Flicker-response enhancement is typically 2-6 times larger at 35 than at 6 Hz. 4. Inspection of flicker waveforms indicates both a scaling-up of response signals with backgrounds and a distortion composed of 2- to 5-ms-latency decrease, expressed primarily within a quick component of OFF-repolarization. 5. Flicker enhancement first increases as a function of background irradiance and then decreases. The increasing limb has the dynamic range and spectral sensitivity of cat rods (507-nm peak). Enhancement is maintained during rod after-effects. The decreasing limb of the background-versus-intensity function results from light adaptation of cat, long-wavelength (red) cones. 6. The flicker responses themselves peak spectrally at approximately 555 nm and reflect only the activity of cat long-wavelength (red) cones, without evidence of intermixing of other photoreceptor mechanisms. 7. Thus within the first synaptic layer of the cat visual system, rod signals interact with the flicker responses of red cones, both increasing cone-signal amplitudes and modifying cone-signal waveforms. 8. The results are closely analogous to "suppressive rod-cone interaction" (SRCI) as described in human psychophysics. 9. An outer-plexiform-layer circuit involving rods, horizontal cells and cones may mediate rod-induced enhancement of cone flicker. This being the case, notions of horizontal-cell feedback interactions with cones may have to be modified and extended. A specific feedback model is elaborated in the companion paper.

Animals↗

PubMed1990

Glial and neuronal markers in bass retinal horizontal and Müller cells.

Retinal horizontal cells (HCs) are second-order neurons that integrate information from photoreceptors over large retinal areas, mediating the lateral spread of visual signals in the distal retina. The 'glial' vs. 'neuronal' nature of the HC has been widely debated. For example, carbonic anhydrase (CA), glutamine synthetase (GS), and glial fibrillary acidic protein (GFAP) are considered 'glial' markers, yet both CA and GFAP have been previously reported in HCs of the teleost retina in species-specific patterns. In contrast, the neurofilament triplet (NFT) proteins are considered 'neuronal' markers; these proteins have been immunolocalized to a mammalian HC, but are absent from teleost HCs. We have studied these cytochemical characteristics in HCs from the white bass, by immunolabeling both cryosections of intact retina and freshly isolated, identified cells attached to coverslips. We found that both HCs (neurons) and Müller cells (MCs; glia) immunolabeled with antisera to CA. Both type 1 (external) HCs and MCs immunolabeled with an antibody to vimentin. Only MCs immunolabeled with antisera to GS and GFAP. Neither HC perikarya (and their major dendrites) nor MCs immunolabeled with an antibody to the 160-kDa subunit of NFT protein. Thus, bass HCs and MCs share the presence of CA and vimentin epitopes and absence of the NFT 160-kDa epitope. Moreover, retinal cell isolation, by itself, does not affect cell-type specific immunolabeling patterns in identified cells, except for what may be lost with the finer processes of the various cells. Isolated cell studies can aid in interpreting immunolabeling patterns observed in the intact retina, especially in retinal layers where several cell types may be present.

Animals↗

PubMed1994

Modulation of electrical synaptic transmission in zebrafish retinal horizontal cells.

Electrical synaptic transmission is widespread in the vertebrate CNS and its modulation plays a critical role in altering the properties of coupled neural networks. In order to define further the mechanisms of electrical synaptic plasticity in the vertebrate retina, the electrophysiological characteristics of solitary horizontal cells and horizontal cell pairs from the zebrafish (Brachydanio rerio) were examined by whole-cell patch-clamp recordings from cells in primary cell culture. In solitary cells, the current-voltage relation exhibited inward current at potentials negative to -60 mV, a linear region of high resistance from -50 mV to 0 mV, and outward current positive to +20 mV. The inward current at negative potentials was blocked by substituting Cs+ for K+ in the extracellular medium, while the outward current at positive potentials was blocked by substitution of Cs+ for K+ in the pipette solution. Measurements of gap junctional conductance from electrically coupled cell pairs revealed that zebrafish horizontal cells expressed a mean junctional conductance of considerably smaller magnitude than other teleost retinal horizontal cells. Junctional conductance was found to be voltage dependent, exhibiting time-dependent closure with increasing transjunctional voltage. Voltage sensitivity was symmetrical about 0 mV junctional potential. At +/- 90 mV the ratio of steady state to peak current was approximately 0.5 and the time constant for inactivation of the junctional current was approximately 120 msec. Junctional conductance was also modulated by dopamine and cAMP. Pairs of horizontal cells responded to puff application of dopamine with a two- to threefold reduction in junctional conductance, but there was no discernible effect on extrajunctional conductances. The action of dopamine on coupling was mimicked by application of the dopamine agonist (+/-)-6,7-dihydroxy-2-amino-tetralin (ADTN) and the membrane permeable cAMP analog 8-bromo-cAMP. The selective D1 dopamine receptor antagonist SCH23390 blocked uncoupling by dopamine. These data provide a primary description of the electrophysiological characteristics of solitary horizontal cells and the electrical coupling between pairs of horizontal cells dissociated from the zebrafish retina. They indicate that zebrafish horizontal cells are distinct from the horizontal cells of other teleosts in their coupling characteristics. The results suggest that zebrafish horizontal cells exhibit differences in the regulation of synaptic assembly and maintenance that have important implications for the function of the zebrafish horizontal cell network in vivo.

Animals↗

PubMed1995

Inwardly rectifying potassium conductance can accelerate the hyperpolarizing response in retinal horizontal cells.

1. We studied the activation properties and assessed the functional role of the inwardly rectifying potassium conductance (GK.IR) in acutely isolated retinal horizontal cells (HCs) with the use of the whole cell patch-clamp technique. 2. The potassium current mediated by GK.IR was isolated by the use of Cs+ or Ba2+ ions. This current was outward, although relatively small in amplitude, in the voltage range between the potassium equilibrium potential (EK) and 50-60 mV more positive. The current reversed its polarity at EK and became inward at potentials more negative than EK. When HCs were bathed in normal Ringer (EK = -90 mV), GK.IR began to active at about -30 mV, was 30-40% activated at the resting potential (-70 to -80 mV) and about fully activated at -130 mV. Thus a significant portion of the activation range of GK.IR overlaps the HC physiological response range (-20 to -80 mV). 3. GK.IR has a dramatic effect on the kinetics of membrane polarization. Blocking GK.IR with Cs+ or Ba2+ significantly slowed the rate of membrane hyperpolarization in response to a hyperpolarizing current ramp over the HC physiological response range. Blocking GK.IR also dramatically slowed the onset rate of a simulated light response generated by a brief break in a sustained glutamate puff. 4. These results suggest that GK.IR can enhance the temporal resolution of the HC by accelerating the onset rate of the hyperpolarizing light response.

Animals↗

PubMed1999

Divalent cations modulate glutamate receptors in retinal horizontal cells of the perch (Perca fluviatilis).

Divalent cations had two effects on concentration-response relations of glutamate induced membrane currents recorded from retinal horizontal cells. The first effect was a reduction of maximum currents. Barium, magnesium, cobalt, nickel and an increased calcium concentration caused reductions of maximum currents between 14% and 70%. The second effect of divalent cations was related to the dopamine dependent modulation of glutamate receptors in horizontal cells. The dopamine dependent enhancement of glutamate gated currents requires the presence of divalent cations besides calcium in the extracellular solution. Without such divalent cations application of dopamine caused no increase of the maximum currents induced by glutamate, and only a slight shift of the half maximal saturation concentration was observed. Addition of magnesium or barium cations in millimolar concentration was sufficient to completely restore the dopamine dependent modulation.

Animals↗

PubMed1995

Retraction of spinule-type neurites from carp retinal horizontal cell dendrites during dark adaptation involves the activation of Ca2+/calmodulin-dependent protein kinase II.

The formation of spinules at the terminal dendrites of retinal horizontal cells with the onset of light and their subsequent retraction during darkness is a remarkable example of synaptic plasticity where sensory experience modifies reversibly, and on a time scale of minutes the ultrastructure of synaptic connectivity. The signals and the subsequent intracellular cascades underlying the prominent morphological alterations are only partially understood. We show here that lowering the external calcium concentration did prevent dark- and AMPA-induced retraction of spinules in a eyecup preparation. Furthermore, spinule retraction was prevented in vivo by the injection of calmidazolium, an inhibitor of calmodulin, into the eyeball, and also by the injection of KN-62, an inhibitor of Ca2+/calmodulin-dependent protein kinase (CaMkII). We conclude that local Ca2+ influx through AMPA-gated channels followed by activation of CaMkII is an important step for spinule retraction during dark adaptation. The phosphorylation patterns of phosphoproteins derived from purified horizontal cells was affected by the inhibitors of calmodulin and CaMkII respectively. Some of the affected phosphoproteins appeared to be cytoskeleton-associated proteins, including GAP-43. Based on these observations, a putative scenario for the retraction of spinules is proposed.

Animals↗

PubMed1984

Electrophysiological effects of GABA on fish retinal horizontal cells are blocked by bicuculline but not by picrotoxin.

We have studied electrophysiologically the actions of gamma-aminobutyric acid (GABA) and related pharmacological agents on fish retinal horizontal cells by recording intracellularly from isolated retinae perfused with Ringer containing the various drugs. We show that although GABA usually hyperpolarizes the membrane potential relative to its dark level, it sometimes and particularly at higher (greater than or equal to 5 mM) concentration produces membrane depolarization, with reduction in the light evoked responses (S-potentials) in both cases. These effects are reversed by bicuculline but not by picrotoxin, although both agents antagonize GABA in many other preparations [5, 25]. The GABA uptake blocker nipecotic acid [15] hyperpolarizes horizontal cells and reduces their light evoked responses, and again these effects are reversed by bicuculline but not by picrotoxin. beta-Alanine, which blocks glial GABA transport [29], and diaminobutyric acid (DABA), which blocks neuronal GABA transport [14, 29, 31], have effects similar to those of nipecotic acid. We discuss these actions of GABA and of the other related drugs and their differential sensitivity to bicuculline and picrotoxin.

Aminobutyrates↗

PubMed1996

Ca(2+)-dependency of spinule plasticity at dendrites of retinal horizontal cells and its possible implication for the functional role of spinules.

Calcium is involved in many aspects of synaptic plasticity and we have analyzed its involvement in spinule dynamics at retinal horizontal cell dendrites. We show here that in particular the retraction of spinules is a Ca(2+)-dependent process. Inhibiting calmodulin or CaMKII, blocked the retraction that was also impaired in low calcium Ringer. Changes of the cytosolic Ca(2+)-concentration through depletion of internal Ca(2+)-stores were without effect. This suggested that Ca(2+)-influx during dark adaption and subsequent activation of CaMKII is an important step for spinule retraction. Voltage dependent Ca(2+)-channels were not responsible for the Ca(2+)-influx, rather Ca2+ leaking through alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate-gated channels. This suggested a close local link between AMPA/kainate receptors and CaMKII indicating a possible postsynaptic function of spinules. The distribution of bound, omega-shaped vesicles within the cone pedicles and its dependence on artificial depolarization further supported the idea of a postsynaptic function of spinules.

Adaptation, Ocular↗

PubMed1989

Dynamics of signal conduction from soma to axon terminal of the teleost retinal horizontal cell: in vivo, in vitro and model studies.

A quantitative study is made on dynamic (frequency-sensitive) properties of the signal conduction between soma and axon terminal of the retinal horizontal cell. Dim flashes of same intensity produced nearly identical responses in soma and axon terminal, indicating that the voltage signal is conducted from soma to axon terminal with little attenuation or distortion. The membrane impedance of axon terminal was measured in solitary-cell preparations under voltage clamp, in order to determine parameters of a resistive-capacitive cable model for the horizontal cell. The simulation analysis shows that passive linear cable models of the present type confront inherent difficulty in predicting dynamical properties of actual flash responses of axon terminal. A nonlinear mechanism is believed to facilitate high-frequency signal conduction along the slender axon that connects soma and axon terminal.

Animals↗

PubMed1989

Enhancement of kainate-gated currents in retinal horizontal cells by cyclic AMP-dependent protein kinase.

Dopamine, acting via cyclic adenosine 3':5'-monophosphate (cAMP), has been shown to enhance a kainate-gated ionic conductance in white perch retinal horizontal cells in vitro. To determine whether this effect involves stimulation of a protein kinase, kainate-gated currents were observed in cultured horizontal cells that were dialyzed with the catalytic subunit of cAMP-dependent protein kinase. Intracellular application of catalytic subunit or cAMP, but not heat-inactivated catalytic subunit, caused significant enhancement of the kainate-evoked currents. These results suggest that kainate-gated channels in horizontal cells may be modified by a phosphorylation event.

Animals↗

PubMed1984

Monoclonal antibodies distinguish subtypes of retinal horizontal cells.

Sixteen hybridomas have been identified that secrete antibodies specific to horizontal cells in the carp retina. The hybridomas have been classified into three groups based on their antibody staining patterns: group I, staining associated with all horizontal cells; group II, staining associated with the most abundant subtype of horizontal cell (CH1); and group III, staining associated with other subtypes of horizontal cells. Most of the hybridomas fall in group II; some of these antibodies stain the entire horizontal cell, but others are specific only to the cell perikarya and do not stain axonal processes. Our results suggest that there are surface molecules specific (i) to all retinal horizontal cells, (ii) to individual subtypes of horizontal cells, and (iii) to portions of horizontal cells. Furthermore, a group II antibody, which recognizes a 48- to 50-kDa membrane protein, has been found to provide a substrate selective for horizontal cell growth. Horizontal cells plated on coverslips coated with this antibody remain healthy in culture and extend long and elaborate processes for at least 3 weeks.

Animals↗

PubMed2000

Odorants suppress voltage-gated currents in retinal horizontal cells in goldfish.

Odorants are known to suppress non-selectively voltage-gated currents in olfactory receptor cells. We found that odorants also suppress voltage-gated currents in neurons of outside of the olfactory system. Under voltage clamp, odorants such as amyl acetate, limonene, and acetophenone suppressed non-selectively voltage-gated currents (a Ca(2+) current, a delayed rectifier K(+) current, a fast transient K(+) current, and an anomalous rectifier K(+) current) in horizontal cells from the goldfish retina. An amyl acetate puff completely and immediately suppressed the Ca(2+) current (I(Ca)) and the delayed rectifier K(+) current induced by repetitive depolarizations, suggesting that amyl acetate is a closed-channel blocker. Odorants did not change significantly the activation curve of I(Ca), but made the slope of inactivation curve of I(Ca) gentler and shifted its half-inactivation voltage toward a negative voltage. These results are similar to the effects of odorants on voltage-gated currents in olfactory receptor cells. This suggests that odorants may suppress the voltage-gated currents in retinal horizontal cells by the same mechanism described in olfactory receptor cells.

Acetophenones↗

PubMed1990

Arginine blocks gap junctions between retinal horizontal cells.

Functions of nitric oxide are of common interest among a variety of tissues, since it activates soluble guanylate cyclase to produce cGMP. Here we report that intracellular application of L-arginine, the precursor of nitric oxide, blocked gap junctions between horizontal cells of the turtle retina. The input resistances of the cells were greatly increased and the cells were thereby easily polarized by current injections through microelectrodes. This procedure enables us to plot precise I-V curves and the reversal potential of light responses was estimated at around 0 mV. These results were quite similar to those obtained by intracellular application of cGMP, suggesting that the L-arginine:nitric oxide:cGMP pathway is present in retinal horizontal cells.

Amino Acids↗