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At least 37 records · Page 2Linked to original sources
PubMed2006

Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production.

Succinic acid is a four-carbon dicarboxylic acid produced as one of the fermentation products of anaerobic metabolism. Based on the complete genome sequence of a capnophilic succinic acid-producing rumen bacterium, Mannheimia succiniciproducens, gene knockout studies were carried out to understand its anaerobic fermentative metabolism and consequently to develop a metabolically engineered strain capable of producing succinic acid without by-product formation. Among three different CO2-fixing metabolic reactions catalyzed by phosphoenolpyruvate (PEP) carboxykinase, PEP carboxylase, and malic enzyme, PEP carboxykinase was the most important for the anaerobic growth of M. succiniciproducens and succinic acid production. Oxaloacetate formed by carboxylation of PEP was found to be converted to succinic acid by three sequential reactions catalyzed by malate dehydrogenase, fumarase, and fumarate reductase. Major metabolic pathways leading to by-product formation were successfully removed by disrupting the ldhA, pflB, pta, and ackA genes. This metabolically engineered LPK7 strain was able to produce 13.4 g/liter of succinic acid from 20 g/liter glucose with little or no formation of acetic, formic, and lactic acids, resulting in a succinic acid yield of 0.97 mol succinic acid per mol glucose. Fed-batch culture of M. succiniciproducens LPK7 with intermittent glucose feeding allowed the production of 52.4 g/liter of succinic acid, with a succinic acid yield of 1.16 mol succinic acid per mol glucose and a succinic acid productivity of 1.8 g/liter/h, which should be useful for industrial production of succinic acid.

Anaerobiosis↗
PubMed2005

Rapid screening procedures for identification of succinic acid producers.

Succinic acid, an intermediate of tricarboxylic acid cycle, is produced and accumulated by anaerobic microorganisms. The long-standing interest in the production of this organic acid is because it is a key compound in producing more than 30 commercially important products. The detection of succinic acid is generally carried out by gas chromatography (GC), enzymatic assays, ion-exclusion chromatography (IEC) or by high performance liquid chromatography (HPLC). However, these methods are time consuming, require sophisticated instrumentation and are expensive. In the present investigation we are reporting two rapid, cost effective screening methods for the detection of this important organic acid. These methods can be utilized to screen a large number of microbes producing succinic acid in a very short span of time.

Bacteria↗
PubMed1999

Sphaeric acid, a new succinic acid derivative from a Sphaeropsis sp.

The structure of sphaeric acid (1), a novel succinic acid derivative isolated from the fermentation broth of a Sphaeropsis sp., was determined by spectral data and synthetic transformation to the diol of sphaeric acid and subsequently to a pair of gamma-lactones (2 and 3).

Fungi↗
PubMedDate not supplied

[The correction of disorders of the internal body environment in animals and man by the addition of succinic acid to food].

Succinic acid is involved in correction of energy metabolism as a powerful source of protons and ATP and as an antioxidant. Some food products contain succinic acid, specifically those, whose preparation involves anaerobic processes, and some berries. However many everyday food products are devoid of succinic acid. The lack of succinic acid in food can be compensated by its use as a food additive. It has been shown that consumption of succinic acid by rats results in a decreased weight increment of adult animals kept on abundant sugar diet. Both therapeutical (higher) and nutritive (lower) doses of succinic acid decrease metabolic acidosis and restore the transaminase activity in patients. The both metabolic parameters indicate correction of energy metabolism.

Animals↗
PubMed1982

Convulsant action of intracerebroventricularly administered l-kynurenine sulphate, quinolinic acid and other derivatives of succinic acid, and effects of amino acids: structure-activity relationships.

Succinic acid, and the derivatives which have been tested, i.e. phthalic, l-glutamic, l-aspartic, citric and laevulinic acids, in doses of 5,2,5,20,20 and 50 micrograms, respectively, as well as l-kynurenine sulphate and quinolinic acid, injected into the brain ventricles in mice, induced clonic seizures. When administered intraperitoneally or orally they antagonize strychnine-induced seizures. The convulsant effect of l-kynurenine sulphate was observed only when the pH of the solution was 4.0 or lower, while the actions of the other drugs tested were not dependent upon pH. Control intraventricular injections of 5 microliters of 0.06 N sulphuric acid (equivalent to 16.7 micrograms of SO4(2-), the same dose as was injected when a convulsant dose of 50 micrograms of l-kynurenine was administered) induced seizures in 25% of the mice. It is suggested that the convulsant effect of quinolinic acid and l-kynurenine sulphate is related to a moiety of succinic acid (O = C-C-C-C = O). The effect of l-kynurenine sulphate comprises the action of the cation of kynurenine and that of the anion of sulphuric acid. Reduction of the convulsant effect of l-kynurenine sulphate by alpha-amino acids and taurine is presumably related to a "shielding" of both amino- and carbonyl groups of 1-kynurenine. There is no evidence that l-kynurenine sulphate, in small doses, shares the central effects of the inhibitory amino acids.

Amino Acids↗
PubMedDate not supplied

[Role of formic acid in transformation of acetic and succinic acids in animal tissues].

Acetic and succinate acids KoA acyl derivatives interacting with formate were displayed to produce alpha-ketoacids--pyruvate and alpha-ketoglutarate. These acids also interact with formate and make pyruvic and malate acids, while alpha-ketoglutarate, evidently, tricarboxy acids. Interaction of formate with acetic and succinate acids inspite of occurring out of the tricarbone cycle increases the latter metabolic functions.

Acetic Acid↗
PubMed1997

Mucosal blood flow and generation of superoxide in rat experimental colitis induced by succinic acid.

As we consider succinic acid to be an exacerbating factor in ulcerative colitis, we investigated its influence on rat colonic mucosa in terms of mucosal blood flow and superoxide generation. We measured mucosal blood flow by the hydrogen gas clearance method and superoxide generation by the chemiluminescence method, and observed histopathological findings to determine the effects of succinic acid. After the instillation of succinic acid of any concentration tested to the colon, mucosal blood flow decreased. Histopathologically, the higher the concentration of succinic acid, the greater was the erosion formation in the colonic mucosa, while significant polymorpho-nuclear cell infiltration superoxide generation from colon tissue were observed with 0.01% succinic acid compared with higher or lower concentrations. Succinic acid, at fecal concentrations found in active stage ulcerative colitis, appears to be implicated in mucosal injury, mediated by a decrease in colonic mucosal blood flow and infiltration of superoxide-generating polymorpho-nuclear cells into the mucosa.

Animals↗