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[Ethical aspects of animal experimentation]

Animal experimentation has become a fundamental part of scientific procedures in the study of living nature. But with the acknowledgement, that animals possess a value of their own, animal experimentation is no longer regarded as self-evidently justified from an ethical point of view. In addition to having at least to make attempts to find alternatives, researchers will also have to embark upon the question of whether the methods used will be compensated by the results of their research. This requires an ability to engage in an ethical dialogue, which should be conducted by ethics committees. Such ethical reasoning may be required to meet certain standards, both with respect to form and content, in order for researchers to be able to maintain that there are "gold reasons" in the ethical sense to pursue their lines of research. Awareness of fallacies (logical pitfalls in ethical argumentation) is important in this dialogue. Public accessibility provides a safeguard for society against neglect of the acknowledgement that animals possess a value of their own.

Journal Article↗

Application of risk assessment to international trade in animals and animal products.

NAFTA and GATT have specified that we must base import decisions on sound scientific information. This includes the use of risk assessment procedures to justify excluding or restricting the international movement of either live animals or products from animals. Procedures and techniques have been described for calculating the risk of almost any hazard that one can imagine. Of particular concern to animal health professionals are the risks associated with introducing known or unknown disease agents into a population of animals that has been previously free of a specific disease. Traditional risk assessment procedures calculate the probability that a disease agent transmission will occur and the cost of such an event. The responsible manager must decide on an acceptable threshold of risk. A set of criteria for determining what risk is acceptable has been developed that categorizes risks for importing animals or animal products from any region of the world based on classification of the region as negligible, slight, low, moderate, high, or unknown risk.

Animal Diseases↗

[Some features of electrical and mechanical stunning in ruminants].

The present studies are concerned with methods of mechanical and electrical stunning as used in ruminants. The object is to acquire a scientific basis for the correct use of these procedures in slaughter-houses. In all experiments studies on living animals, cerebral activity (electro-encephalogram) was measured using electrodes which had been surgically implanted the previous day. When animals are stunned by concussion, unconsciousness is not induced in every case. When a slaughtering mask was frontally or occipitally applied to the head, unconsciousness was produced. To effectively induce unconsciousness, the bolt of the captive bolt pistol should carry sufficient energy to damage the cerebral cortex and the deeper portions of the brain directly or by shock waves following penetration of the cranium. The forces which may be developed by the variously coloured cartridges of the brands of captive bolt pistol differ markedly. Regulations regarding the relationship between the colour of the cartridge and the potential forces would appear to be essential.

Abattoirs↗

VAMOS--ventilator assisted model for off-pump surgery.

BACKGROUND: Introduction of innovative cardiac procedures mostly depends on extensive evaluation using laboratory animal models. Avoiding living creatures in the experimental setup where possible without loss of reliability would be progressive from a logistical as wells as an ethical perspective. METHODS: Hearts of meat-production animals (pigs) were removed without structural damage. Two tracheotomy tubes (length 35 mm, diameter 10 mm) were inserted into aortic and pulmonal positions. The atrial inflow had to be ligated. The ventilation tubes were joined to a respirator. The "ventilation" frequency was limited to a maximum of 100/min, "inspiration" volume at 100 ml and ventilation pressure at up to 60 mbar. RESULTS: "Air beats" triggered by the frequency of the respirator occurred in the ventilated heart. Insufflation and deflation generated movement that was almost the same as physiological action. CONCLUSIONS: Air-animation of the heart using a standard respirator is an easily conducted alternative to extensive and expensive laboratory experiments for development and quality control of new devices or techniques without compromising the scientific value of results and without using living animals.

Animals↗

Pig organs for transplantation into humans: a Jewish view.

In view of the shortage of human organs for transplantation, intense interest has focused on the use of pig organs. Although the early rejection of pig organs by a human recipient has not yet been overcome, scientists are actively seeking to solve this problem. If and when xenotransplantation from pigs or other animals becomes scientifically feasible, Judaism will look with favor upon this procedure to prolong or save the life of a human being who is ill or dying from organ failure. Although Jewish law forbids Jews to raise or eat pigs, no such prohibition exists for the use of pigs to cure human illness or to save human lives by xenotransplantation.

Animals↗

Transrectal ultrasound guided manipulation of the canine prostate with minimum intervention.

The canine prostate is a widely used and well established animal model for the assessment of therapeutic effects of laser technology in the search for better options for the treatment of benign prostatic hyperplasia. Conventionally for such experiments, the canine prostate is approached by laparotomy or transperineal urethrotomy. We have demonstrated a technique involving the use of ultrasound guided biopsy of the liver and prostate, and percutaneous laser treatment of the prostate, which has proved to be effective in reducing adverse effects on the experimental animals but without compromising scientific requirements for the experiments. We conclude that state-of-the-art percutaneous procedures not only refine animal intervention significantly but are also technically feasible for most laser studies using the canine prostate as an experimental model in the live animal.

Animals↗

Final finality in livings from ancient philosophy and medicine to contemporary biomedicine.

In recent years biotechnology has introduced into medicine new possibilities for the manipulation of forms of life, including in vitro fertilization, production of transgenic animals and use of human fetal tissues for therapeutic procedures. This has given rise to a debate on final finality of the beginning human life, which has involved scientific, philosophical, religious as well as legal issues. A report by an English governmental Commission (Warnock Committee, 1984) stated a practical term, e.g. 14 days, during which the so-called preembryo may be useful for research or therapeutics. The time has been chosen on embryological bases (i.e. appearance of nerve-type cells in the primitive streak), whereas a law is claimed to avoid discussions about the time of appearance of sensitivity of embryos. On the other side is the opinion of the Court for Blount County of Tennessee expressed in the rule Davis vs. Davis (1989). While the term preembryo is considered a false distinction between stages of differentiation of human embryos, human life is considered beginning at conception and human embryos not as property. A comparative analysis of medical and philosophical thought before the influence of Christianity shows that, for Hippocrates as well as for Aristotle, final finality of the early embryo leads to consider the human being as a person just when life is blooding. This phase occurs after the milk one or vegetative stage, which is characterized by the property of doubling life when cells are separated, the same stage considered in the rule Davis vs. Davis.

Embryology↗

A longitudinal study of veterinary students and recent graduates. 4. Gender issues.

OBJECTIVE: To study differences in experiences, attitudes and opinions between female and male veterinary students and recent graduates. DESIGN: Longitudinal study. POPULATION: Students-77 males and 77 females-who began studying veterinary science at The University of Queensland in 1985 and 1986. PROCEDURE: Questionnaires were completed in the first and fifth year of the course, and in the second year after graduation. The data were analysed using the SAS System for Windows. RESULTS: Females decided to study veterinary science at a younger age than males and were more influenced by 'a love of animals', the image of veterinarians as portrayed on television, an interest in living things and in the scientific study of disease. Males were more influenced than females in aspects of the workplace: bosses and money. There were no gender differences in their background in relation to farms, or to animals, or in their career plans. However females when in first year expected a lower initial income than males; an expectation that was realised in the first year after graduation. As first year students and also as veterinarians, females had stronger views than males on animal welfare issues, and also felt that the veterinary profession had a lower status relative to other professions. CONCLUSIONS: Significant differences in attitudes and experiences exist between males and females entering the veterinary profession. The situation of females in relation to income and status is consistent with that in other professions, where females have been disadvantaged compared with males.

Age Factors↗

[What education means in surgery? A surgery among chirurgical residents in university hospital in Strasbourg].

OBJECTIVE: Numerous tools for surgical education are available. The objective of the study is to know how future surgeons do feel about these different possibilities to learn and what do they think about the place of e-learning, or virtual universities and on-line training and learning in their formation. METHODS: A questionnaire has been sent to each surgical student of Strasbourg University. Learning and training methods were evaluated: observation, fellowship, Internet, reading videos, reading books or scientific journals, discovery of pathology by oneself or performing surgical procedures by oneself, sharing meetings, playing pedagogic games. RESULTS: Fellowship is voted by plebiscite, as using Internet which grows the usual book contents thanks to interactivity, videos, quickenings and actuality of the inquiries. The difficulties to approach this tool is emphasized : the cost, availability, time consuming, lack of any tutor, ability with a computer. Dissection of corpses or live animals is wished by the majority of young surgeons. Using simulators or robots remains something for the future and must be evaluated. CONCLUSION: Fellowship is voted by plebiscite for learning surgery. The interest for new technologies of communication is more out of curiosity than necessity.

Adult↗

Genetically engineered vaccines for control of Aujeszky's disease (pseudorabies).

A gene-deleted pseudorabies (PR) (Aujeszky's disease) vaccine, named OMNIVAC-PRV, was licensed by the United States Department of Agriculture-Animal and Plant Health Inspection Service (USDA-APHIS) on January 16, 1986, the first recombinant DNA-derived modified-live virus vaccine to be licensed for manufacture and sale anywhere in the world. Two months later, a second generation gene-deleted 'marker' vaccine, OMNIMARK-PRV, was described. Surprisingly, these landmark applications of genetic engineering to vaccine development triggered a brouhaha and a lawsuit (ultimately dismissed by Federal District Court Judge Thomas Hogan) as well as a Congressional Hearing on the adequacy of USDA regulatory procedures for the licensing of supposedly 'dangerous' recombinant DNA-derived products. Thus, its is rather gratifying that only 4 years later, a consensus of government, industry, veterinarians and the scientific community has developed, affirming that the recombinant DNA-derived vaccines were in fact a major breakthrough for pseudorabies (PR) control and eradication, and that without the advantages of gene-deleted vaccines, plans for PR eradication would not be practical.

Animals↗

[I. P. Pavlov on vivisection and the modern ethical requirements for animal experiments].

It is shown that Pavlov's opinion is in agreement with the modern requirements for experiments in living animals: use of living animals in experiments is recommended only in the cases when there is no alternative and scientific-practical significance of the planned experiments is justified; minimization (as much as possible) of discomfort, distress and pain of animals under study without sacrifice of the quality of scientific research; use of appropriate number of animals necessary for obtaining reliable results which are adequate to a given experimental situation and general state of the animals inadmissibility of using extra number of animals; drawing up a protocol concerning the experimental procedure and results; reflection in the protocol the techniques of sedation, anesthesia, and euthanasia; constant care of the improvement of the theoretical and practical qualification of a researcher in the ethical questions and experimental technique. Pavlov's views led his time and at present they agree with modern concepts on the experimental ethics.

Animal Welfare↗

Apparatus for measuring rat body volume: a methodological proposition.

We propose a communicating-vessels system to measure body volume in live rats through water level detection by hydrostatic weighing. The reproducibility, accuracy, linearity, and reliability of this apparatus were evaluated in two tests using previously weighed water or six aluminum cylinders of known volume after proper system calibration. The applicability of this apparatus to measurement of live animals (Wistar rats) was tested in a transversal experiment with five rats, anesthetized and nonanesthetized. We took 18 measurements of the volume under each condition (anesthetized and nonanesthetized), totaling 90 measurements. The addition of water volumes (50-700 ml) produced a regression equation with a slope of 1.0006 +/- 0.0017, intercept of 0.75 +/- 0.81 (R(2) = 0.99999, standard error of estimate = 0.58 ml), and bias of approximately 1 ml. The differences between cylinders of known volumes and volumes calculated by the system were <0.4 ml. Mean volume errors were 0.01-0.07%. Among the live models, the difference between the volumes obtained for anesthetized and nonanesthetized rats was 0.31 +/- 2.34 (SD) ml (n = 90). These data showed that animal movement does not interfere with the volume measured by the proposed apparatus, and neither anesthesia nor fur shaving is needed for this procedure. Nevertheless, some effort should be taken to eliminate air bubbles trapped in the apparatus or the fur. The proposed apparatus for measuring rat body volume is inexpensive and may be useful for a range of scientific purposes.

Animals↗

Why do in utero stem cell transplants sometimes fail?

In utero stem cell transplantation promises a novel therapeutic approach to those genetic disorders that can be diagnosed in early pregnancy and that could lead to either severe disability or death. Available scientific evidence suggests that such procedures could achieve clinically relevant levels of engraftment with donor cells and that the resulting sustained chimerism is potentially long lived. However, the relatively few cases performed so far have not borne out initial hopes, and both the source and the type of cell preparation to be transplanted and the choice of disorders to target with this therapy remain controversial.

Animals↗

[Is brain surgery on primates in basic research morally acceptable?]

In the contemporary controversy about the legitimacy of vivisection a few basic assumptions are shared by nearly all participants of the discussion. (I) Pure Research in the service of medicine is of great value for humankind. It contributes to prolonging human life and the alleviation and prevention of human suffering. (II) Brain surgery for the sole purpose of pure research is morally unacceptable in the case of any human being. (III) Primates are sensitive beings which lead a rich social life and are endowed with remarkable intellectual capacities. (IV) Primates have a moral standing, possibly to a lesser degree compared with human beings, certain acts are therefore an injustice toward them. The controversy then is about the question whether premise (I) outweighs (IV), i.e. whether the benefit of the pure research is from a moral point of view more important than the suffering of innocent primates. I shall present four arguments against such a conclusion. 1) According to premise (I) brain surgery on human beings for the sole purpose of pure research is morally unacceptable. Since this prohibition is meant to include all human beings it cannot rest on the exclusive human possession of reason because e.g. some mentally handicapped human beings lack this faculty. All other properties which may be named as basis for the ascription of a moral status which forbids brain surgery for pure research, are possessed also by some animals, especially primates; therefore it is impossible to deny them the same moral status. 2) Brain surgery on primates is confronted with an insoluble dilemma: If the characteristics of the primate brain are very similar to that of human beings, the scientific benefit is obvious, but the procedure appears to be morally unacceptable exactly because of this similarity. If, on the other hand, the characteristics differ significantly, brain surgery may seem legitimate but the scientific benefit becomes doubtful at best. 3) We could quite easily save hundreds of human lives if e.g. speed limits would be reduced (say) by half. Most of us, however, are unwilling to accept such a loss of quality of life in order to save a certain number of human lives. Since we are no prepared to pay this comparatively modest price, we have, in my eyes, no moral right to impose considerable pain and suffering on a primate to save human lives. 4) Pure research in the service of human medicine is from a moral point of view of great importance. Since most of the work in this area is done or financed by private corporations and not by state institutions, from a economical point of view the aim consists in making profit. Since the latter aspect has gained more and more weight in the last years the moral worth of pure research cannot rule out any other moral concern.

Journal Article↗

Transfusion via the carotid artery in the hamster.

Public health policy makers need quantitative scientific data to assess the risk to the blood supply posed by transmissible spongiform encephalopathy (TSE) diseases. To this end, our laboratory has developed a model of blood-borne TSE infectivity in hamsters infected with the 263K strain of scrapie, an experimental model of choice for quantitative studies of TSE infectivity. We report here a microsurgical method for cannulation of the carotid artery in the hamster that allows transfusion of a large fraction of the blood volume of the hamster, with virtually no blood loss to the surgical site or exposure to nervous tissue. Animals are minimally affected by the surgery, recover quickly and completely, and survive for their natural lives (as long as 3 years). This procedure has been used to obtain quantitative data on the transmissibility of the TSEs by transfusion, and these findings have informed public health policy on blood donation and blood use.

Animals↗

Analysis of survival data on aging rat cohorts: pitfalls and some practical considerations.

Experimental aging research is very dependent on the determination of the survival characteristics of the animal species or strain under study. Such data are generally inferred from mortality curves of cohorts of animals that are set aside at an early age for aging studies. Rectangular survival curves and the presence of multiple pathological lesions are a prerequisite for aging studies so as to resemble the situation in man. From 1977 onwards, many rat cohorts have been formed in the Institute for Experimental Gerontology (IVEG) for the study of aging processes. Data from these have been analysed for a period of 5 years up to and including 1982. (Males and females of the WAG/Rij and BN/BiRij strains were used.) The 50% survival and the maximum survival of cohorts varied considerably, but showed no consistent trend over the years. The median (50%) survival between the cohorts differed by as much as 7.9-10.7 months for the strains and sexes studied. Maximum survival between the cohorts varied from 3.7 to 9.9 months. Median and maximal survival were greater for the females. Maximum survival and 50% survival correlated significantly, the relation between the two being approximately linear. The effect of removing animals from cohorts on the estimation of 50% survival was only minor, whereas maximum survival was clearly diminished by this procedure. The wide variation in survival characteristics, even between successive cohorts, cautions against too simple a measure of the animals survival in only one number for median or maximal survival in months. An indication of the variance of 50% survival and of maximum survival should therefore be included in scientific publications. Moreover, the 50% survival is the parameter of choice to define cohorts, not only because this can be most reliably estimated with good confidence limits, but also because this measure is the least sensitive to removing animals from the cohorts. As this will often be the case in many research institutions, it might be of practical importance to order old animals from different cohorts since this diminishes the chance of using an extremely short or long lived cohort. Finally, the analysis revealed that combining intact or incomplete cohorts into larger survival curves resulted in nearly identical graphs. An attempt was made to calculate the minimum cohort size which yields survival curves with constant 95% confidence limits.

Aging↗

Training, credentialling, and granting of clinical privileges for laparoscopic general surgery.

Despite the lack of scientific data comparing it with traditional open operations, laparoscopic surgery has gained rapid acceptance and implementation by general surgeons. Individual hospitals, which have the responsibility for developing their own privileging criteria, are searching for guidance as to the amount and type of additional training required to grant clinical privileges in laparoscopic general surgery. Laparoscopic surgery involves techniques different from those learned during general surgery residency training. Therefore, until such techniques are regularly included in general surgery residency programs, additional training for and granting of separate privileges in laparoscopic surgery are appropriate. Adequate training for surgeons already experienced in abdominal and biliary tract surgery can be acquired through a preceptorship in diagnostic laparoscopy, attending a course in laparoscopic surgery that includes both didactic instruction and live animal experience, assisting with the procedures in humans, and being proctored and certified as competent by an experienced general surgeon.

Animals↗

Colonization capability of lactobacilli and pathogens in the respiratory tract of mice: microbiological, cytological, structural, and ultrastructural studies.

Respiratory tract infections are among the bacterial infections that affect humans with higher frequency. Those produced by Streptococcus pneumoniae are reported to have the highest incidence in the world, affecting both children and old people. As a 2001 report from the World Health Organization expressed it, the basic fight of children under 5 yr old is to survive. Five different conditions (acute respiratory infections, diarrhea, measles, palludism, and undernutrition) directly produce more than 50% of the deaths in this age group. Respiratory tract infections in the developing countries in the Americas are among the first three causes of death in children under 1 yr and between the first and second cause in children between 1 and 4 yr old. Pneumonia is responsible for 85 and 90% of deaths in children under 5 yr old (approx 150,000 annually), 95% of them occurring in the developing countries in the Americas. There is an increased worldwide tendency to use preventive measures and to consume products that help to maintain the health status of the individual. Thus the use of probiotics has increased systematically during the last decade, and the scientific literature trying to demonstrate the positive effect of such preparations has also increased. The term probiotic has been applied to products that (1) contain live microorganisms, freeze-dried or included in fermented products or (2) improve the health status of humans and animals, exerting effects in the mouth or gastrointestinal tract (included in foods or capsules), in the respiratory tract (as aerosols), or in the urogenital tract (by local application)Having in mind the high incidence of respiratory tract infections, and looking for preventive measures as well as the possible applications of probiotics, the aim of this chapter was to use mice as experimental models to determine whether members of the genus Lactobacillus were able to colonize and give protection from infections after inoculation by the intranasal route. To this end, the following procedures were carried out: 1. Screening of the predominant bacterial species in respiratory organs. 2. Study of the kinetics of colonization of the different groups of microorganisms from 15 d up to adult (2 mo). 3. Screening of the probiotic characteristics of all the isolated strains.

Animals↗