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Dentin permeation: comparison of diffusion with filtration.

A comparison was made between the rate of iodide permeation of dentin by diffusion and by filtration. Before acid etching, filtration only doubled the rate of iodide permeation relative to that obtained by diffusion. After acid etching, filtration produced a 32-fold increase in permeation. Acid etching, by removing occluding debris, facilitates filtration more than diffusion.

Acid Etching, Dental

Dentin permeability: changes produced by iontophoresis.

Iontophoretic currents (0--1.0 mA) were applied to dentin disks prepared from human third molars. A negative electrode increased 125I-permeation 10X, while a positive electrode increased 14C-lidocaine 8X. Iontophoresis appears promising as a method to increase the permeation of dentin by ionized substances of therapeutic interest.

Carbon Radioisotopes

Dentin permeability: a comparison of functional versus anatomical tubular radii.

Two independent techniques (filtration and surface tension) were developed to measure the functional tubular radii of 11 dentin and 2 glass discs. Anatomic radii were determined using SEM for comparative purposes. The functional radii of the dentin discs ranged from 5 to 40% of the anatomic radii. This differences was due to the fact that SEM visualizes only the surface while the functional techniques measure the radii within dentin tubules. It is concluded that the functional methods are preferred whenever the dynamics of fluid flow through dentin are under consideration.

Acid Etching, Dental

Permeability of the hard tissue bridge formed after pulpotomy with calcium hydroxide: a histologic study.

Analysis of these results shows that complete hard tissue bridges, aside from occurring with great frequency, produce a satisfactory protection to the pulp. In a case in which the barrier is incomplete, some future clinical problems may result as an incomplete barrier does not offer adequate protection as shown by the chronic inflammatory reaction in the remaining pulp tissue.

Animals

A method for the study of internal spaces in hard tissue matrices by SEM, with special reference to dentine.

Infiltration of a porous hard tissue with methyl methacrylate, followed by dissolution of the tissue provides a three-dimensional methacrylate cast of spaces within the tissue. Examination of such a cast by scanning electron microscopy provides information on the nature and extent of the pore system to a degree that cannot readily be visualized by the direct examination of fractured surfaces. The findings described concern human dentine but the technique may have a wider application in studies on the internal structure of other hard tissues.

Dental Enamel Permeability

Rate of permeation of isotopes through human dentin, in vitro.

A simple in vitro method has been described for quantitatively measuring the rate at which isotopically labeled substances permeate through dentin in occlusal cavity preparations. The presence or absence of pulp tissue had only a minor effect on the kinetics of permeation. As the dentin was made thinner there was a resulting increase in the rate of iodide permeation. The relative rates of permeation were 3H2O greater than 131I greater than 99mTc greater than 14C-urea, a sequence that follows the molecular dimensions of these substances.

Carbon Radioisotopes