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Reproducibility of clinical caries diagnoses on coronal and root surfaces.

Intra- and interexaminer reproducibility of clinical caries diagnoses was studied using 3 experienced dentists, who independently examined 20 patients twice at an interval of 2-6 weeks. The lesions were classified as one of the following four diagnoses: (1) initial active caries, (2) initial inactive caries, (3) manifest active caries, and (4) manifest inactive caries. For the various diagnoses, signs of cavitation, surface structure and discolouration were used. The reproducibility at a patient level was evaluated using intraclass correlation coefficients and at a surface level using kappa statistics. At a patient level, for the total number of lesions, the intraclass coefficients of correlation varied from 0.55 to 0.77. For the individual diagnoses, total manifest caries showed the highest coefficients of correlation, ranging from 0.73 to 0.92. At a tooth surface level, the kappa values varied from 0.29 to 0.61. The most common shift was that from any type of caries diagnosis to a diagnosis of a sound surface. There was little difference between the results for coronal and root caries and between initial and manifest lesions. Nor was there any difference for buccal + lingual surfaces only compared to all surfaces. Judging from the pattern of change in diagnosis between the repeated examinations, the main source of error seemed to be due to the fact that the lesions observed at one examination were overlooked at the other examination.

Adult↗

Caries prevention by CO2 laser treatment: dependency on the number of pulses used.

The aim of this study was to assess the caries-preventive potential of various carbon dioxide laser conditions and to explore the effect of the number of laser pulses used. The authors irradiated unerupted human molars at two wavelengths--10.6 or 9.6 micrometers--and at 1, 5, 25 or 100 pulses. All teeth were then subjected to pH cycling simulating the conditions for caries progression. Pulsed CO2 laser-preventive treatment inhibited caries-like lesion progression by up to 87 percent. This effect was dependent on the number of pulses used, but there was no correlation between caries resistance and enamel surface morphological changes.

Acetates↗

Assessing the effect of fluoride varnish on early enamel carious lesions in the primary dentition.

BACKGROUND: The aim of this study was to evaluate the effect of fluoride varnish on enamel caries progression in the primary dentition. METHODS: One hundred forty-two children in Head Start schools (3 to 5 years old) were randomized into the varnish and control groups. Children in the varnish group received fluoride varnish (Duraphat, Colgate-Palmolive Co.) at baseline and after four months, and children in the control group received no professional fluoride applications. Two calibrated examiners performed the examinations at baseline and at nine months. RESULTS: At nine months, the authors found that in the control group, 37.8 percent of active enamel lesions on occlusal, buccal and lingual surfaces became inactive, 3.6 percent progressed and 36.9 percent did not change. In the varnish group, 81.2 percent became inactive, 2.4 percent progressed and 8.2 percent did not change. The difference between the groups was statistically significant (P < .0001). The mean decayed surfaces, or ds, value in the varnish group was significantly lower after nine months than it was at baseline (P < .0001). When enamel lesions were included in the data analysis (along with dentinal lesions), the decayed with initial enamel lesions, missing and filled surfaces, or dEmfs, values; decayed with initial enamel lesions, missing and filled teeth, or dEmft, values; and decayed surfaces with initial enamel lesions, or dEs, values were significantly lower in the varnish group after nine months than they were at baseline (P < .0001). CONCLUSIONS: These results suggest that fluoride varnish applications may be an effective measure in reversing active pit-and-fissure enamel lesions in the primary dentition. CLINICAL IMPLICATIONS: Fluoride varnishes are safe, easy to apply and well-accepted by patients. This study shows that fluoride varnish may offer an efficient, nonsurgical alternative for the treatment of decay in children.

Child, Preschool↗

In vitro evaluation of secondary caries development in enamel and root dentin around luted metallic restoration.

Materials that release fluoride have been recommended for patients with high caries risk, but there is no conclusive evidence of their effect on fixed prostheses abutments. This study evaluated the influence of a resin-modified glass-ionomer cement on in vitro caries development in enamel and root dentin around metallic restoration. The cervical portion of 12 human third molars were sectioned in four blocks that were randomly divided into four Groups (n = 12; 1 block from each tooth/group): ZP (restoration + zinc phosphate cement), GI (restoration + resin-modified glass-ionomer cement), C1 (no restoration and no pH cycling), and C2 (no restoration and pH cycling). In ZP and GI, metallic restorations were luted at the crown-root junction. ZP, GI and C2 were submitted to a pH-cycling model. All blocks were sectioned, embedded and polished. Enamel and root dentin demineralization were evaluated through cross-sectional microhardness Knoop measures at 20 to 80 microns of depth (from the outer surface) and at 20 to 220 microns of distance from the cavity margins. Mineral loss values were calculated from Knoop hardness numbers. Data were analyzed by ANOVA, Tukey's test and regression analysis. Groups ZP, GI and C2 showed demineralization in relation to C1, which was more superficial in enamel than in root dentin. In root dentin, mineral loss in ZP and GI was smaller than in C2. Distance from the cavity margins was not significant. There was no difference between resin-modified glass-ionomer cement and zinc phosphate cement regarding artificial secondary caries development in both dental substrates.

Analysis of Variance↗

Histopathology of the pulp of primary molars with active and arrested dentinal caries.

The purpose of this study was to compare the histological appearance of the pulp of human primary molars with active and arrested lesions. The sample consisted of 36 primary molars (18 with active lesions and 18 with arrested lesions) extracted from 35 children between 5 to 9 years of age. The histological diagnosis was classified in normal pulp, transitional stage, partial pulpitis, total pulpitis and total necrosis, and then subdivided in three subgroups: treatable, untreatable and questionable. Results showed that normal pulp or transitional stage (treatable category) was diagnosed in 50% of teeth with arrested lesions, compared to 11.1% of teeth with active lesions. Partial pulpitis (questionable category) was present in 38.8% with arrested lesions compared to 22.2% with active lesions. Total pulpitis and total necrosis (untreatable category) was diagnosed in 11.2% with arrested lesions compared to 66.7% with active lesions. The observed frequencies of histological categories between both groups were statistically significant (P < 0.05). Histologically, pulp reaction under active and arrested lesions in primary molars revealed the formation of a basophilic calcio-traumatic line at the junction of the primary and reparative dentin, formation of reparative dentin and a regular odontoblastic layer in 60% of the cases. Results indicated that the type of lesion (active or arrested) is a good indicator of the histological status of the pulp.

Basophils↗

A model for clinical evaluation of the effect of antimicrobial agents on carious dentin.

PURPOSE: To evaluate, longitudinally, the effect of a chlorhexidine varnish on the proteolytic activity of dentin caries in vivo. MATERIALS AND METHODS: 20 permanent molars and 8 primary molars with carious lesions in dentin were studied in subjects 18-35 yrs old (n=20), and 5-6 yrs old (n=8) respectively. These lesions were clinically evaluated according to texture and color. Carious dentin specimens were obtained by means of biopsies performed with a #4 carbide bur at the initial visit (TO) before application of a 10% chlorhexidine varnish and 2, 4, 8, and 12 wks thereafter. The dentin biopsies were immersed in Sorensen's buffer, vortexed for 30 s, and mixed with a 1.67 mM solution of n-benzoyl-DL-arginine-naphthylamide (BANA), a substrate for proteolytic enzymes. Samples were incubated overnight at 37 degrees C and color was developed with 0.1% fast garnet. The optical density (OD) of reaction mixtures was recorded photometrically. All teeth were grouped for analysis, as Mann-Whitney tests revealed no statistically significant differences between median values for OD for both age groups. ANOVA was used to compare progressive inhibition of proteolytic activity in dentin caries samples over time. RESULTS: The average proteolytic activity at the dentin substrates (OD) at TO and 2, 4, 8 and 12 wks thereafter were 0.794+/-0.089, 0.741+/-0.071, 0.676+/-0.087, 0.600+/-0.094, and 0.508+/-0.108 respectively. The chlorhexidine varnish mediated a significant inhibition of the proteolytic activity present in dentin caries after 12 wks (P<0.0001). At T0, 100% of the carious lesions examined were characterized as soft upon exploration. After 12 wks, 54% (15/28) of the lesions were partially hardened and 46% (13/28) hardened/nonprogressing. The dentin color was yellow/light brown in 100% of the lesions at baseline, and dark brown/black in 86% (24/28) after 12 wks. CLINICAL SIGNIFICANCE: This study demonstrated that chlorhexidine varnishes arrested active caries in vivo and inhibited the proteolytic activity present in these lesions. These findings strengthen the rationale for including chlorhexidine in the overall treatment strategy for patients with high caries activity.

Adolescent↗

"Cariogenic potential of homemade weaning diet v/s commercial formula feeds" an in-vitro study.

The universal phenomenon of introducing weaning diet to the growing child sets its onset by the age of 4-6 months. This preliminary diet of the child governs his or her dental health in the long run. The main objective of this study is to investigate the histopathological and clinical invasion of deciduous teeth exposed to both the homemade weaning diet and commercial formula feeds. The study also aims at motivating the consumption of the most appropriate diet for the weaning baby. Extracted deciduous teeth were incubated with the test solutions of homemade diet and commercial formula feeds for the period of 6 weeks. Fewer carious invasion both clinically and histopathologically were seen in homemade feeds. Thus, homemade diet can be a strong candidate for the status of 'THE IDEAL WEANING FOOD'.

Animals↗

In vitro model for evaluating the effect of child formula toothpastes on artificial caries in primary dentition enamel.

PURPOSE: To determine if the in vitro 10-day pH-cycling model used for permanent teeth could be utilized to evaluate de/remineralization effects, on the enamel of primary teeth, of child formula toothpastes. METHODS: Sound extracted primary anterior teeth were coated with nail varnish, leaving a 1 mm-wide window prior to being placed in a demineralizing solution to produce artificial carious lesions in enamel. The teeth were subsequently cut longitudinally into 100 microm thick sections. The 72 specimens were randomly assigned to six groups. In Set 1 contained the specimens in Group a, which were treated with a pea-sized (0.32 g) quantity of non-fluoride toothpaste (First Teeth), and Groups b and c which were treated with half and pea-sized (0.16 g) quantities of fluoridated toothpaste (Colgate), and cycled for 10 days. The specimens in Set 2 (Groups A, B and C) were similarly treated but subjected to 7-day pH-cycling. RESULTS: After Day 8 the lesions in Set 1 extended into dentin and so could not be evaluated. Polarized light microscopy and microradiography were used to evaluate the lesions subjected to 7-day pH-cycling. Lesions in Groups A and B increased in depth and area by approximately 50%, while those in Group C increased in depth and area by 20%; however, no statistically significant differences occurred between the groups. If this model pH cycling is to be used for primary teeth, a reduction from 10 to 7 days in length should be considered.

Cariostatic Agents↗

Effect of amine fluoride (AmF)/stannous fluoride (SnF2) toothpaste and mouthwashes on dental plaque accumulation, gingivitis and root-surface caries.

The aim of the study was to assess the effect of a toothpaste and mouthwashes containing amine fluoride (AmF) and stannous fluoride (SnF2) on dental plaque, gingivitis and root-surface caries. Forty-four adults participated in a five-month double-blind study. The following combinations of toothpastes and mouthwashes were used: (1) AmF/SnF2 toothpaste and AmF/SnF2 (Meridol) mouthwash (20 patients, mean age 45.7 years), (2) sodium fluoride (NaF) toothpaste and NaF mouthwash (24 patients, mean age 48.8 years). The mean values for dental plaque (Silness-Löe index) were clinically and statistically significantly reduced in both groups, the difference being greater in the AmF/SnF2 group. Sulcus-bleeding index values were clinically and statistically significantly decreased in both groups. There was no marked difference between the groups. The root caries index (RCl, Katz) had decreased by the end of the experiment by 10% in the NaF group and by 47.4% in the AmF/SnF2 group.

Amines↗

Fluoride and caries prevention: 2. Clinical rationale.

In order to achieve the greatest clinical benefit from the use of fluoride, we must understand its mechanism of action, so that fluoride can be given at the right time, by the best route, at the optimum dosage and in the most suitable form. The first article in this two-part series reviewed the scientific rationale behind the use of fluoride. Part 2 now illustrates fluoride therapy in practical terms, by examining nine clinical cases.

Adolescent↗

Enamel and dentin demineralization inhibition of fluoride-releasing materials.

Fluoride-releasing dental restorative materials may provide an additional benefit in preventive dentistry. Although not currently available in the United States, a fluoride-releasing amalgam has demonstrated recurrent caries inhibition at enamel and dentin restoration margins. Likewise, both chemical-cured and light-cured glass ionomer cements have demonstrated caries inhibition at enamel and dentin restoration margins. Fluoride-releasing resin composites have also consistently demonstrated recurrent caries inhibition at enamel margins, yet there are conflicting results whether caries inhibition occurs at dentin margins. Preliminary studies indicate that glass ionomer cement and fluoride-releasing resin composite have synergistic effects with fluoride rinses and fluoridated dentifrices, in the remineralization of incipient enamel caries. The materials may act as a fluoride delivery system. Upon exposure to additional external fluoride, the material surface undergoes an increase in fluoride. This fluoride is subsequently released and has demonstrated demineralization inhibition and even remineralization at adjacent tooth structure. Clinical research to evaluate these fluoride-releasing restorative materials may provide further information for clinical recommendations.

Composite Resins↗

Strengthening of tooth enamel by a remineralizing toothpaste after exposure to an acidic soft drink.

The effect of remineralizing and conventional toothpaste treatments on the hardness of intact and acid soft drink-etched enamel were assessed in a laboratory study. The remineralizing toothpaste (Enamelon Toothpaste) used in the study contains NaF, and simultaneously provides dissolved calcium, phosphate and fluoride ions. The conventional toothpaste contains NaF in a silica base. Sound extracted maxillary human incisors, mounted in epoxy resin with the facial incisal two-thirds exposed, were polished with 0.3 mu alumina. Groups of ten teeth were exposed to either twenty 5-minute treatments with an acid soft drink (pH 2.4), remineralizing or conventional fluoride toothpaste, or to twenty alternating cycles of a 5-minute protective treatment with either the remineralizing or conventional toothpastes, followed by 5-minute exposures to the acid soft drink, or to 20 five-minute exposures to the acid soft drink followed by 20 five-minute restorative treatments with the remineralizing or conventional toothpastes. Knoop Hardness measurements were made before and after treatment using a 500 g load and 15-second dwell time. Acid soft drink exposure produced a 15.4% drop in hardness compared with 4.9% and 1.6% hardness increases due to treatments of intact enamel with the remineralizing and conventional toothpastes, respectively. Protective treatments using the remineralizing and conventional toothpastes significantly reduced the drop in hardness due to acid soft drink exposure to 3.3% and 6.2%, respectively. Restorative treatments by the remineralizing and conventional toothpastes significantly increased the hardness of the acid soft drink-weakened enamel by 12.1% and 7.3%, respectively. Both toothpastes were effective in inhibiting damage due to acid soft drink exposure, but the remineralizing toothpaste was more effective in hardening intact and decalcified enamel than the conventional toothpaste (p < 0.05).

Analysis of Variance↗

Fluoride remineralization of demineralized bovine tooth enamel and hydroxyapatite pellets.

Both bovine enamel and hydroxyapatite pellets were remineralized in a fluoride-containing remineralization solution after prior demineralization for various lengths of time. In both the enamel and pellet systems, the degree of remineralization attainable was directly related to the extent of prior demineralization, although the demineralized material was never 100% recovered in remineralization. In some cases, fluoride levels up to several thousand parts per million were found at depths as great as 50 micrometers from the surface. The stoichiometry of the remineralized material and electron microprobe examination were consistent with the formation of fluoridated hydroxyapatite rather than calcium fluoride.

Animals↗

Reversal of primary root caries using a dentifrice with a high fluoride content.

Root caries is a widespread problem and can be quite serious in older populations. The restoration of root carious lesions is often difficult. Compared to enamel caries, there has been limited research into the pharmaceutical management of primary root caries lesions (PRCLs), and many of these studies have been carried out in vitro, with limited numbers of clinical trials. Fluoride is presently a cornerstone in dentifrice formulations for cost-effective and anticaries therapy. It is generally accepted that fluoride ions promote remineralization of tooth substances and reduce the rate of demineralization. The use of a dentifrice with a high fluoride content may be considered to reverse PRCLs, since more fluoride is required for the remineralization of roots than for enamel. This paper reviews the effects of dentifrices with high fluoride contents on the management of root caries.

Adult↗

Effect of intermittent delivery of fluoride to solution on root hard-tissue de- and remineralization measured by 125I absorptiometry.

The effect of intermittent fluoride levels on root hard-tissue de- and remineralization was studied once daily for 21 days in a pH-cycling caries model with simulated fluoride clearance curves. Four root hard-tissue blocks, from each of 12 human teeth, were cut out parallel to the cementum surface. During a daily 15-hour period, the blocks were subjected 12 times to pH changes similar to those which occur in plaque after a carbohydrate intake. The fluoride was delivered immediately before a daily nine-hour remineralization period. Four experiments were independently carried out: One block from each tooth was subjected to pH-cycling without and with fluoride delivery, simulating a rinse with 0.025, 0.2, and 1.0% sodium fluoride (NaF), respectively. The mineral change in the blocks was monitored by 125I absorptiometry and expressed as the change in transmission (delta T). The surface between the data points (delta T values) and the x axis (time points) was used as a summary measure, i.e., the area under the curve (AUC). When no fluoride was delivered, the delta T increased over 21 days, indicating loss of mineral. The AUC was, on average, 5.85 +/- 0.68 (mean +/- S.E.) %.day. In the 0.025% NaF-rinse experiment, there was a marked reduction in mineral loss, indicated by an average AUC of 1.66 +/- 0.59%.day. In both the 0.2 and 1.0% NaF-rinse experiments, a decrease in delta T, indicating gain of mineral, was observed from day 2. Negative delta T values occurred after 7 and 3 days, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Analysis of Variance↗

Monitoring the caries process. Optical methods for clinical diagnosis and quantification of enamel caries.

Early detection of a caries lesion would enable the dentist, by using effective prophylactic measures, to enhance remineralization and conservation of the tooth substance rather than restoration of the dentition. The presently-used diagnostic methods of detecting early lesions require visual observation. Reflected light is used to detect changes in color, texture, and translucency of the tooth substance. Attempts to improve the traditional methods or to develop new ones have been numerous. For clinical use, only 2 quantitative methods are presently available: (i) a method based on the scattering of light by enamel crystals in relation to their surrounding environment, and (ii) a method that uses visible laser light within the blue-green region as the light source to excite the autofluorescence of enamel. The possibilities and limitations of these methods are evaluated, and clinical applications are presented and discussed.

Color↗