PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Water Purification”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 37 records · Page 2Linked to original sources

Chemical resistance of the gram-negative bacteria to different sanitizers in a water purification system.

BACKGROUND: Purified water for pharmaceutical purposes must be free of microbial contamination and pyrogens. Even with the additional sanitary and disinfecting treatments applied to the system (sequential operational stages), Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were isolated and identified from a thirteen-stage purification system. To evaluate the efficacy of the chemical agents used in the disinfecting process along with those used to adjust chemical characteristics of the system, over the identified bacteria, the kinetic parameter of killing time (D-value) necessary to inactivate 90% of the initial bioburden (decimal reduction time) was experimentally determined. METHODS: Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas picketti, Flavobacterium aureum, Acinetobacter lowffi and Pseudomonas diminuta were called in house (wild) bacteria. Pseudomonas diminuta ATCC 11568, Pseudomonas alcaligenes INCQS , Pseudomonas aeruginosa ATCC 15442, Pseudomonas fluorescens ATCC 3178, Pseudomonas picketti ATCC 5031, Bacillus subtilis ATCC 937 and Escherichia coli ATCC 25922 were used as 'standard' bacteria to evaluate resistance at 25 degrees C against either 0.5% citric acid, 0.5% hydrochloric acid, 70% ethanol, 0.5% sodium bisulfite, 0.4% sodium hydroxide, 0.5% sodium hypochlorite, or a mixture of 2.2% hydrogen peroxide (H2O2) and 0.45% peracetic acid. RESULTS: The efficacy of the sanitizers varied with concentration and contact time to reduce decimal logarithmic (log10) population (n cycles). To kill 90% of the initial population (or one log10 cycle), the necessary time (D-value) was for P. aeruginosa into: (i) 0.5% citric acid, D = 3.8 min; (ii) 0.5% hydrochloric acid, D = 6.9 min; (iii) 70% ethanol, D = 9.7 min; (iv) 0.5% sodium bisulfite, D = 5.3 min; (v) 0.4% sodium hydroxide, D = 14.2 min; (vi) 0.5% sodium hypochlorite, D = 7.9 min; (vii) mixture of hydrogen peroxide (2.2%) plus peracetic acid (0.45%), D = 5.5 min. CONCLUSION: The contact time of 180 min of the system with the mixture of H2O2+ peracetic acid, a total theoretical reduction of 6 log10 cycles was attained in the water purified storage tank and distribution loop. The contact time between the water purification system (WPS) and the sanitary agents should be reviewed to reach sufficient bioburden reduction (over 6 log10).

Disinfectants↗

[Bacterial regrowth in drinking water. IV. Bacterial flora in fresh and stagnant water in drinking water purification and in the drinking water distribution system].

Six dead end water pipes were installed inside a Zurich drinking water plant and five others over a distance of 12 km along the distribution system and the water was left stagnating in there for 2 weeks. A total of 1508 bacteria from fresh and stagnating water were isolated and identified. Of these, 241 bacterial isolates from the distribution system were examined using the nutrient-tolerance test, i.e. testing the ability to grow in tap water and in media with low and very high nutrient content. In the fresh water of the treatment plant specific bacterial populations were obtained, these occurring particularly after the filters. According to different chlorine dosage and chlorine demand, they were finally washed into the distribution system in varying amounts and compositions. It was shown that in the fresh water of the distribution system the genera of Pseudomonas, Azotobacter and Actinobacteria were each present at a level of approximately 30%. After two weeks stagnation non-fluorescing pseudomonads were dominating in the treatment plant as well as in the fresh water of the distribution system. All isolated Actinobacteria and Azotobacter and almost half of the Pseudomonads proved to be oligotrophic oligocarbotolerants or oligocarbophilic organisms in the nutrient-tolerance test. The other half of the Pseudomonads plus the Flexibacter species were mesotrophic oligocarbotolerants, since they could grow in tap water and in culture media with very high nutrient content. Attention is drawn to the unrecognized danger of recontamination of mesotrophic bacteria growing rapidly in stagnating drinking water, which is used as rinsing water for cleaning food processing equipment.

Acinetobacter↗

Solid olive waste in environmental cleanup: oil recovery and carbon production for water purification.

A potentially-economic three-fold strategy, to use solid olive wastes in water purification, is presented. Firstly, oil remaining in solid waste (higher than 5% of waste) was recovered by the Soxhlet extraction technique, which can be useful for the soap industry. Secondly, the remaining solid was processed to yield relatively high-surface area active carbon (AC). Thirdly, the resulting carbon was employed to reversibly adsorb chromate ions from water, aiming to establish a water purification process with reusable AC. The technique used here enabled oil recovery together with the production of a clean solid, suitable for making AC. This process also has the advantage of low production cost.

Adsorption↗

Electrochemical alkaline Fe(VI) water purification and remediation.

Fe(VI) is an unusual and strongly oxidizing form of iron, which provides a potentially less hazardous water-purifying agent than chlorine. A novel on-line electrochemical Fe(VI) water purification methodology is introduced. Fe(VI) addition had been a barrier to its effective use in water remediation, because solid Fe(VI) salts require complex (costly) syntheses steps and solutions of Fe(VI) decompose. Online electrochemical Fe(VI) water purification avoids these limitations, in which Fe(VI) is directly prepared in solution from an iron anode as the FeO42- ion, and is added to the contaminant stream. Added FeO42- decomposes, by oxidizing a wide range of water contaminants including sulfides (demonstrated in this study) and other sulfur-containing compounds, cyanides (demonstrated in this study), arsenic (demonstrated in this study), ammonia and other nitrogen-containing compounds (previously demonstrated), a wide range of organics (phenol demonstrated in this study), algae, and viruses (each previously demonstrated).

Electrochemistry↗

An evaluation of a water purification system for use in animal facilities.

A commercially available water purification system was evaluated for its ability to minimize chemical and microbial contaminants. The reduction or removal of these impurities from the drinking water of experimental animals would reduce experimental variability. 3 strains of bacteria were collected from the processed water. An increase in the total number of bacteria was observed the longer the filters remained in use. Determinations of heavy metals in water samples before and after processing were made for lead, zinc, copper, nickel, manganese, iron, arsenic and mercury. Calcium and magnesium levels were also determined. The concentrations of these inorganic chemicals were reduced by the purification process except at 2 time points in which desorption of the chemical could have occurred. Bacterial colonization and desorption of these chemicals were controlled by installing new filter cartridges. Volatile halocarbon concentrations were determined for water samples before and after purification. All volatile halocarbons analyzed were less than 10 ppb before and after purification at all time points. Other organic chemicals were greatly reduced by the purification process. In a study of contaminants associated with installation of the unit, it was found that flushing the unit for 8 days reduced lead and methyl ethyl ketone concentrations to insignificant levels. The purification system was found to be effective in providing high quality drinking water as verified by a microbial and chemical testing program.

Animals↗

Community structure of microbial biofilms associated with membrane-based water purification processes as revealed using a polyphasic approach.

The microbial communities of membrane biofilms occurring in two full-scale water purification processes employing microfiltration (MF) and reverse osmosis (RO) membranes were characterized using a polyphasic approach that employed bacterial cultivation, 16S rDNA clone library and fluorescence in situ hybridization techniques. All methods showed that the alpha-Proteobacteria was the largest microbial fraction in the samples, followed by the gamma-Proteobacteria. This suggested that members of these two groups could be responsible for the biofouling on the membranes studied. Furthermore, the microbial community structures between the MF and RO samples were considerably different in composition of the most predominant 16S rDNA clones and bacterial isolates from the alpha-Proteobacteria and only shared two common groups ( Bradyrhizobium, Bosea) out of more than 17 different bacterial groups observed. The MF and RO samples further contained Planctomycetes and Fibroacter/ Acidobacteria as the second predominant bacterial clones, respectively, and differed in minor bacterial clones and isolates. The community structure differences were mainly attributed to differences in feed water, process configurations and operating environments, such as the pressure and hydrodynamic conditions present in the water purification systems.

Alphaproteobacteria↗

Water purification system by using the biofilter for long-term experiment equipment with aquatic animals for the space station.

We have developed a water purification system that enables long-term experiment with aquatic animals for 90 days or more on the space station. We designed the system that combined a biofilter for ammonia removal (nitrification) with another for nitrate removal (denitrification). The experiment with goldfish was for 90 days with an aquatic animals' examination device. The equipment consists of a fish tank, a filter module, pumps, and an artificial lung for gas exchange. The goldfish were kept in the tank without any water replacement throughout the experiment. When a filter module consists of adsorbents without bacteria, the concentration of the nitrite and ammonia begin to increase so that the goldfish die. On the contrary, neither ammonia nor nitrite accumulated throughout the experiment, and the concentration of T-N also maintained 30 ppm or less when the combined biofilter was used. Moreover, no fish died throughout the period. The water purification system with biofilter enabled us to examine the long-term life support testing. We also report a new denitrification (correction of dentrification) method for the life support system.

Animals↗

[Immobilized microorganisms and water purification].

Advantages and disadvantages of cells of aerobic microorganisms immobilized by the type of adhesion and incorporation into the gel beads, the amount of retained biomass, limitations of diffusion of oxygen and nutrients, viability, morphology, biochemical properties are described. Immobilized biocatalysts are discussed in the aspect of their use in purification of sewage waters.

Bacteria, Aerobic↗

Aquatic ecosystem as a bioreactor: water purification and some other functions.

A fundamental concept is proposed of aquatic ecosystem as a bioreactor that carries out the function of water purification in natural water bodies and streams. The ecosystem as a bioreactor has the following characteristic attributes: (1) it is a large-scale (large-volume) bioreactor; (2) it is a diversified (in terms of the number of taxa and the scope of functional activities) bioreactor; (3) it possesses a broad range of biocatalytic (chemical-transforming and degrading) capabilities. New experimental data on xenobiotics-induced inhibition of the functioning of the molluscs Unio tu- midus, U. pictorum, M. galloprovincialis and Limnaea stagnalis emphasize the potential ecological hazard from sublethal concentrations of pollutants (including those exemplified by synthetic surfactants and detergents).

Animals↗

An iodine load from water-purification tablets alters thyroid function in humans.

Tablets containing tetraglycine hydroperiodide are used to purify small quantities of water for drinking. Because short-term administration of stable iodide can alter thyroid function in normal adults, we evaluated the effects of these tablets on thyroid function during a military field training exercise. Fourteen normal volunteers participated in the study. After an adjustment period of 2 weeks to the field environment, half of the subjects received four dissolved water-purification tablets (32 mg free iodine) daily for 7 consecutive days while the other half served as controls. At the end of the study the treatment group showed reductions in the mean serum levels of thyroxine (14%) and total triiodothyronine (15%) and significant increments in basal thyrotropin (TSH) (122%) and the TSH response to thyrotropin-releasing hormone (49%) compared to baseline values. The basal TSH level rose above the normal range in two subjects. No significant changes in any parameter of thyroid function were observed in the control subjects. One week of daily exposure to the iodine load from four tetraglycine hydroperiodide water-purification tablets causes mild impairment of thyroid function in humans.

Adolescent↗

Do iodine water purification tablets provide an effective barrier against Cryptosporidium parvum?

U.S. Army Iodine Water Purification Tablets were tested to determine their efficacy against Cryptosporidium parvum, a protozoan resistant to chemical disinfection. Purified oocysts in phosphate-buffered water were treated with varying concentrations of iodine or with iodine tablets as per U.S. Army protocol. Neonatal mouse pups were then each inoculated with 10,000 treated oocysts, and 1 week later scored as infected or uninfected. Using this methodology, iodine tablets were found to be inadequate against C. parvum because the Army doctrinal dose of 560 mg min/L, calculated as 16 mg of I2/L and 35 minutes of contact time, showed less than 1 log inactivation. A dose of 29 mg of I2/L at the same contact time was required to achieve a 2 log inactivation.

Animals↗

Occurrence of Cryptosporidium oocysts and Giardia cysts in a conventional water purification plant.

A one-year monitoring of Cryptosporidium oocysts and Giardia cysts was conducted at a water purification plant. A total of thirteen 50 L samples of river source water and twenty-six 2,000 L samples of filtered water (treated by coagulation-flocculation, sedimentation and rapid filtration) were concentrated using a hollow fibre ultrafiltration membrane module at a purification plant. Cryptosporidium oocysts were detected in all raw water samples with a geometric mean concentration of 400 oocysts/m3 (range 160-1,500 oocysts/m3). Giardia cysts were detected in 12/13 raw waters (92%) with a geometric mean concentration of 170 cysts/m3 (range 40-580 oocysts/m3). Probability distributions of both Cryptosporidium oocysts and Giardia cyst concentration in raw water were nearly log-normal. In filtered water samples, Cryptosporidium oocysts were detected in 9/26 samples (35%) with a geometric mean concentration of 1.2 oocysts/m3 (range 0.5-8 oocysts/m3) and Giardia cysts in three samples (12%) with 0.8 cysts/m3 (range 0.5-2 oocysts/m3). The estimated removal of Cryptosporidium oocysts and Giardia cysts was, respectively, 2.54 log 10 and 2.53 log10 on the basis of geometric means, 3.20 and 3.57 log10 on the basis of 50% observation level and 2.70 and 2.90 log10 on the basis of 90% observation level.

Animals↗

Elimination of organophosphate ester flame retardants and plasticizers in drinking water purification.

Organophosphate ester flame retardants and plasticizers like tris-(2-chloro-, 1-methyl-ethyl)phosphate (TCPP), tris-(2-chloro-, 1-chloromethyl-ethyl)phosphate (TDCP), tris-(2-chloroethyl)phosphate (TCEP), tributylphosphates, triphenylphosphate (TPP), ethylhexyldiphenylphosphate (EHDPP) and tris-(butoxyethyl)phosphate (TBEP) have been studied in diverse processes for drinking water purification. The elimination efficiency of these different treatment processes, e.g., biological active slow underground passage, soil passage and technical treatment processes such as ozonization or multilayer and activated carbon filtration have been studied in three waterworks in the catchment area of the river Ruhr. In the untreated surface water the concentrations of the chlorinated organophosphates ranged 50-150 ng L(-1) TCPP, 10-130 ng L(-1) TCEP and 10-40 ng L(-1) TDCP. The amounts of the non-chlorinated alkylphosphates were in the same order of magnitude (40 ng L(-1) of the tributylphosphates, 170 ng L(-1) of TBEP and 10 ng L(-1) TPP) depending on weather and water flow. EHDPP was detected in the range of 1 ng L(-1). After the drinking water purification process in all waterworks in this study, the concentrations of the selected substances were below the respective limit of quantification (0.3-3 ng L(-1)). While activated carbon filtration as well as extended passage through soil (10-15 days residence time) were effective in eliminating all selected compounds, ozonization and multilayer filtration did not contribute to the elimination of the chlorinated compounds. The elimination effect of slow underground passage combined with soil passage concerning the halogenated compounds seemed to depend on the hydraulic residence time.

Filtration↗

[Combined use of active chlorine and coagulants for drinking water purification and disinfection].

The authors made an experimental study of the efficiency of water purification procedures based on the combined use of active chlorine and coagulants and hygienically evaluated the procedures. The study included the evaluation of water disinfection with various coagulants and active chlorine; the investigation of the processes of production of deleterious organic chlorine compounds; the assessment of the quality of water after its treatment. The coagulants representing aluminum polyoxychloride: RAX-10 (AQUA-AURATE 10) and RAX-18 (AQUA-AURATE 18), and aluminum sulfate, technically pure grade were tested. The treatment of river water with the coagulants RAX-10 and RAX-18, followed by precipitation, filtration, and chlorination under laboratory conditions, was shown to result in water disinfection to the levels complying with the requirements described in SanPiN 2.1.4.1074-01. RAX-18 showed the best disinfecting activity against total and heat-tolerant coliform bacteria, but also to the highly chlorine-resistant microrganisms--the spores of sulfite-reducing Clostridia, phages, and viruses. Since the coagulants have an increased sorptive capacity relative to humus and other organic substances, substitution of primary chlorination for coagulant treatment may induce a reduction in the risk of formation of oncogenically and mutagenically hazardous chlorinated hydrocarbons.

Chlorine↗

Limited effectiveness of home drinking water purification efforts in Karachi, Pakistan.

OBJECTIVE: In many developing-country urban areas, municipally supplied water is not microbiologically safe. This study evaluated drinking water quality and effect of home water purification efforts in Karachi, Pakistan. METHODS: Members of 300 households, including 100 households who used the Aga Khan University Hospital Laboratory and 200 of their neighbors were interviewed. In 293 consenting households, structured observations were performed and drinking water was analyzed for the presence of coliforms, using the multiple tube fermentation technique. RESULTS: Although 193 of the 293 households (66%) reported using some method to purify their drinking water, including 169 (58%) who boiled their water, only 48 (16%) of the drinking water samples were free of coliforms. Although a combination of boiling and filtering was the most effective method of purification, only 38% of samples that had been boiled and filtered were free of coliforms. CONCLUSIONS: Further refinements and evaluations of home-based efforts to purify and store water are needed.

Developing Countries↗

Reduction of fecal contamination of street-vended beverages in Guatemala by a simple system for water purification and storage, handwashing, and beverage storage.

Street-vended foods and beverages, an integral part of urban economies in the developing world, have been implicated in cholera transmission in Latin America. To improve the microbiologic quality of market-vended beverages in Guatemala, we tested a simple system consisting of dilute bleach (4.95% free available chlorine) for water purification, narrow-mouth plastic vessels with spigots for disinfecting and storing water and for preparing and storing beverages, handwashing soap, and education in using the system. We conducted a randomized controlled intervention trial among 41 vendors who received the intervention and 42 control vendors, comparing total and fecal coliform bacteria and Escherichia coli contamination of market-vended beverages, stored water, and vendors' hands. Samples were obtained at baseline and at each of six weekly follow-up visits. At baseline, fecal coliform bacteria were found in 40 (48%) market-vended beverages and E. coli in 14 (17%). When compared with samples from control vendors, a significant decrease in total coliform (P < 0.001) and fecal coliform (P < 0.001) bacteria in samples of stored water and beverages sold by intervention vendors was observed over the course of the study. The vessel system was well accepted by vendors. This simple inexpensive system consisting of hypochlorite disinfectant, plastic vessels, soap, and education can significantly reduce fecal contamination of market-vended beverages.

Adolescent↗

Identification of bacteria in drinking and purified water during the monitoring of a typical water purification system.

BACKGROUND: A typical purification system that provides purified water which meets ionic and organic chemical standards, must be protected from microbial proliferation to minimize cross-contamination for use in cleaning and preparations in pharmaceutical industries and in health environments. METHODOLOGY: Samples of water were taken directly from the public distribution water tank at twelve different stages of a typical purification system were analyzed for the identification of isolated bacteria. Two miniature kits were used: (i) identification system (api 20 NE, Bio-Mérieux) for non-enteric and non-fermenting gram-negative rods; and (ii) identification system (BBL crystal, Becton and Dickson) for enteric and non-fermenting gram-negative rods. The efficiency of the chemical sanitizers used in the stages of the system, over the isolated and identified bacteria in the sampling water, was evaluated by the minimum inhibitory concentration (MIC) method. RESULTS: The 78 isolated colonies were identified as the following bacteria genera: Pseudomonas, Flavobacterium and Acinetobacter. According to the miniature kits used in the identification, there was a prevalence of isolation of P. aeruginosa 32.05%, P. picketti (Ralstonia picketti) 23.08%, P. vesiculares 12.82%,P. diminuta 11.54%, F. aureum 6.42%, P. fluorescens 5.13%, A. lwoffi 2.56%, P. putida 2.56%, P. alcaligenes 1.28%, P. paucimobilis 1.28%, and F. multivorum 1.28%. CONCLUSIONS: We found that research was required for the identification of gram-negative non-fermenting bacteria, which were isolated from drinking water and water purification systems, since Pseudomonas genera represents opportunistic pathogens which disperse and adhere easily to surfaces, forming a biofilm which interferes with the cleaning and disinfection procedures in hospital and industrial environments.

Acinetobacter↗