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Do brain oscillations of different frequencies indicate interaction between cortical areas in humans?

Oscillatory cortical activity in the beta frequency range (14-32 Hz) was investigated in a voluntary movement task using closely-spaced electroencephalogram recordings over the sensorimotor cortex. According to recent studies, short-lasting beta oscillations were observed within the first second after termination of a self-paced finger movement. In this study we show, moreover, that finger movement creates beta bursts not only overlying the hand representation area, but also, at a higher frequency, over neighboring cortical areas representing the foot. The results indicate that, related to the same event, distinct cortical areas can display increased synchronized activity at different frequencies, providing evidence for interaction between the corresponding neuronal networks.

Action Potentials↗

MuLV packaging systems as models for estimating/measuring retrovirus recombination frequency.

Interaction of retrovirus vectors and endogenous retroviruses present in packaging cell lines and target cells may result in the formation of recombinant viruses. Using sensitive RT-PCR assays, we have investigated human and murine gene therapy packaging cell lines for the incorporation of endogenous retrovirus transcripts into murine leukaemia virus (MLV) vector particles and whether vector genomes are incorporated into human endogenous retrovirus (HERV) particles. VL30 endogenous retrovirus sequences were packaged in particles produced by the murine AM12 packaging system. For every seven MLV-derived -galactosidase beta-Gal vector genomes present in the particles, one copy of VL30 was also packaged. Although human FLY packaging cells expressed HERV transcripts (HERV-K, HuRT, type C, and RTVL-H), none was detectable in the MLV vector particles released from the cells. Non-specific packaging of the MLV gag-pol expression vector transcripts was detected in the FLY virions at a low level (one in 17,000 sequences). In other experiments, gag proteins produced by HERV-K particles present in human teratocarcinoma cells did not appear to package MLV-based vectors that expressed Gal transcripts. These findings indicate that retrovirus vectors interact with human packaging cells to produce retrovirus particles that are far less contaminated by endogenous viral sequences or other types of extraneous particles than murine packaging cells.

Animals↗

Use of perches and nestboxes by laying hens in relation to social status, based on examination of consistency of ranking orders and frequency of interaction.

Four groups of 15-19 adult ISA Brown hens were studied in pens to assess the relationship between social status and use of perches and nestboxes. This was to test the hypothesis that subordinate hens use these resources more by day, for avoiding dominants, but that dominants use perches more at night, for roosting. The experiment consisted of a 5-week pre-treatment period, when no perches were present, and a 4-week treatment period, when each group was tested with different perch treatments (No, Low, Medium, High). All groups were observed systematically in each week, when all interactions of three types (aggressive peck, non-aggressive peck, approach/avoidance) in a group were recorded by noting the instigator and recipient (from numbered wing tags) onto a matrix. Proportions of time that each bird spent using perches and nestboxes, by day and at night, were also recorded. The results indicate that social status of individual laying hens is relatively stable across time and can be based reliably on counts of either aggressive pecks or approach/avoidances, but not non-aggressive pecks. Aggressive pecks were the most frequent type of interaction observed, and were reduced by the presence of perches. Use of nestboxes, but not perches, was greater at night than by day. There were weak tendencies for perches, and to a lesser extent nestboxes, to be used more by lower ranking birds by day, but not at night. There was some evidence of increased use of these resources by higher ranking birds at night. It is concluded that provision of perches reduces bird density on the floor (where nearly all interactions occurred), allows subordinates a means of avoiding dominants by day, reduces frequency of agonistic interactions, and should thus benefit laying hen welfare.

Journal Article↗

[Familiarity, favorability, and cognitive complexity: integration of frequency of interaction hypothesis and vigilance hypothesis].

Previous studies using Bieri's 'cognitive complexity' score had supported 'vigilance hypothesis' which assumed that impressions of unfavorable persons were more complex than favorable persons. Thus, Bieri's measure seemed to be invalid because the findings were completely contrary to 'frequency of interaction hypothesis', presented in the theoretical framework of 'cognitive complexity', which assumed that impressions of familiar persons were more complex than unfamiliar persons, since people could be supposed to have more intimate acquaintance with favorable persons. The purpose of present study was to indicate the invalidity was caused by the research design where familiarity was dependent on favorability and to show that even Bieri's score could support 'interaction hypothesis', if one variables could be statistically orthogonized to the other. In each of two surveys reported, about two hundred female undergraduates completed a Rep test where they rated favorable and unfavorable persons on the basis of some dimensions which included favorability and familiarity. The results obtained through various regression analyses supported the above predictions. Moreover, it was revealed that, with favorability controlled, Bieri's score showed almost linear increases as familiarity increased, though the score of extremely unfamiliar persons was rather higher than the score the linear function could predict.

Adult↗

Widely separated spatial frequencies: mechanism interactions.

The question of independence between spatially tuned mechanisms that respond to very different frequencies is addressed by a theoretical reconstruction of their responses to single and compound grating stimuli presented at near-threshold levels. Parameters of sensory response distributions are estimated in a two-response rating experiment, and tested against those predicted by a Signal Detection model when independent processing is assumed. Small but consistent deviations from predicted values were observed and are interpreted in terms of frequency-specific patterns of inhibition and stochastic correlation. It is further demonstrated that these interactions may in part account for the superiority of discrimination over detection found with such widely separated frequencies.

Discrimination, Psychological↗

Naming cAsE aLtErNaTeD words.

A dual-route approach was used as an initial framework to examine the relation between presentation format and lexical processing in a naming task. In Experiments 1 and 3, words were presented in lowercase versus case-alternated format. Presentation format interacted with word frequency and regularity: For irregular words (e.g., pint), case alternation was additive with frequency, whereas for regular words (e.g., mint), case alternation and frequency interacted. Experiment 2 dissociated the locus of case-alternation effects from those of stimulus intensity. Stimulus intensity was additive with frequency and regularity, suggesting that whereas stimulus intensity affects encoding, case alternation affects lexical processing at a postencoding stage in the word recognition system. It was concluded that a dual-route approach provides a suggestive but incomplete account of how case alternation influences lexical processing. As an alternative to a dual-route approach, we show that the present results can be addressed and successfully simulated using an implemented version of Norris's (1994) multilevel model.

Female↗

Levels of Ly-49 receptor expression are determined by the frequency of interactions with MHC ligands: evidence against receptor calibration to a "useful" level.

Ly-49 receptor expression was studied in NK cells that developed in fully MHC-mismatched mixed bone marrow chimeras, in which host and donor MHC ligands were expressed solely on various proportions of hemopoietic cells or on both hemopoietic and nonhemopoietic cells. When hemopoietic cells were the only source of MHC ligand, a strong correlation between the level of down-regulation of Ly-49A, Ly-49C, and Ly-49G2 and the number of hemopoietic cells expressing their MHC ligands was observed on both donor and host NK cells. In some animals with low levels of donor hemopoietic chimerism, NK cells of donor origin expressed Ly-49 receptors at higher levels than was observed in normal mice of the same strain. This unexpected observation is inconsistent with the receptor calibration theory, which states that expression of Ly-49 inhibitory receptors is calibrated to an optimal level to maintain an NK cell repertoire that is sensitive to perturbations in normal class I ligand expression. Our data suggest a model in which Ly-49 receptors down-modulate in accordance with the frequency of their interactions with ligand-bearing cells, rather than a model in which these receptors calibrate to a specific "useful" level in response to ligands present in their environment.

Animals↗

Measuring specialization in species interaction networks.

BACKGROUND: Network analyses of plant-animal interactions hold valuable biological information. They are often used to quantify the degree of specialization between partners, but usually based on qualitative indices such as 'connectance' or number of links. These measures ignore interaction frequencies or sampling intensity, and strongly depend on network size. RESULTS: Here we introduce two quantitative indices using interaction frequencies to describe the degree of specialization, based on information theory. The first measure (d') describes the degree of interaction specialization at the species level, while the second measure (H2') characterizes the degree of specialization or partitioning among two parties in the entire network. Both indices are mathematically related and derived from Shannon entropy. The species-level index d' can be used to analyze variation within networks, while H2' as a network-level index is useful for comparisons across different interaction webs. Analyses of two published pollinator networks identified differences and features that have not been detected with previous approaches. For instance, plants and pollinators within a network differed in their average degree of specialization (weighted mean d'), and the correlation between specialization of pollinators and their relative abundance also differed between the webs. Rarefied sampling effort in both networks and null model simulations suggest that H2' is not affected by network size or sampling intensity. CONCLUSION: Quantitative analyses reflect properties of interaction networks more appropriately than previous qualitative attempts, and are robust against variation in sampling intensity, network size and symmetry. These measures will improve our understanding of patterns of specialization within and across networks from a broad spectrum of biological interactions.

Adaptation, Physiological↗

Neighborhood effects on nonword visual processing in a language with shallow orthography.

Neighborhood size and neighborhood frequency were orthogonally varied in two experiments on Italian nonwords. In Experiment 1, an inhibitory effect of neighborhood frequency on visual lexical decision was found: The presence of one high-frequency neighbor increased response latencies and error rates to nonwords. By contrast, no effect of neighborhood size and no neighborhood size x neighborhood frequency interaction were found. In Experiment 2, a facilitatory effect of neighborhood size on nonword naming was shown: Naming latencies were faster when nonwords had a large neighborhood. In the naming experiment, there was no effect of neighbors' frequency and no neighborhood size x neighborhood frequency interaction. An additional role for bigram frequency was found whereas syllable frequency did not give any independent contribution. These results further corroborate the view that, in a language with transparent orthography like Italian, despite a substantial contribution of sublexical print-to-sound mapping due to the language's high regularity/consistency, reading aloud of nonlexical material may benefit from the contribution of the lexical component.

Humans↗

Frequencies and interactions of regulatory T cells. I. The balance between help and suppression regulates the primary immune response to keyhole limpet hemocyanin in vitro.

Frequencies and efficiencies of regulatory T cells from non-immunized mice were estimated in several assay systems differing from each other in cellular composition and antigen dose (NIP-KLH). The NIP-specific and total IgM responses were quantified. Using 10(4) syngeneic B cells and 50 micrograms/ml NIP-KLH, helper (Th) cells from 5 day immune donors were detected in frequencies of 1:3000-1:4000 in lymphnode and spleen T cells, with an efficiency of 70-90 ng IgM/Th cell in C57B1/6 mice. In non-immune spleen T cells, Th cells were observed in frequencies of 1:16,000-1:38,000, with comparable efficiency, but these Th cells appeared suppressed at increased T cell doses. Polyclonal activation led to the appearance of multiple independently regulated populations of Th cells with similar efficiencies. In the presence of 10(5) syngeneic spleen cells, treated once with anti-Thy-1 antibody and complement and 50 micrograms/ml NIP-KLH, suppressor activity was observed in the same T cell population. Similar to help, suppression fluctuated with increasing T cell numbers. Using 1 x 10(6) spleen cells and 50 micrograms/ml NIP-KLH as assay system, T cells enhanced the responses. Again, several independently regulated populations were observed, with efficiencies slightly higher than those of the above-described Th cells. By maintaining the cellular components of the assay system constant (10(4) B cells) and titrating the antigen, Th cell frequencies showed little variation up to 100 micrograms/ml NIP-KLH and were always suppressed at higher T cell numbers. At 200 micrograms/ml NIP-KLH, the frequency was increased to approximately 1:2000, and not suppressed, i.e., was identical to the frequency observed in mice immunized 5 days previously. Efficiencies increased with increasing doses of antigen. The results strongly indicate that regulatory T cell function shows "plasticity", in the sense that the appearance and the frequencies of helping and suppressing T cell populations highly depend on the micro-environment present in culture.

Animals↗

Spatial frequency specific interaction of dot patterns and gratings.

Adaptation to patterns of paired random dots produces loss of contrast sensitivity to sinusoidal luminance gratings oriented perpendicularly to the dot-pair direction. This adaptation loss is spatial frequency- and orientation-specific and varies with dot-pair separation in a manner predictable from the Fourier spectra of the stimuli and observed characteristics of the visual system. These results support the idea that the visual system acts as a periodicity analyzer with known restrictions and cannot be accounted for by a feature-detector model. When the bars of the test gratings are aligned in the dot-pair direction, there is no adaptational loss at any frequency despite the fact that the adaptation pattern contains significant spectral power at all frequencies in this orientation. This lack of adaptation may be due to inhibitory interactions among channels or to nonlinear effects within local receptive fields.

Adaptation, Physiological↗

Atrial inotropic responses to brief vagal stimuli: frequency-force interactions.

The effect of brief vagal stimulus burst on atrial contractile force was assessed in paced- and unpaced-heart preparations by recording the changes in pressure (AP) generated in a balloon in the right atrial appendage or by a miniature strain gauge on the atrium. One and 3 stimuli per vagal burst depressed AP up to 62 and 90%, respectively. The response varied with the time in the cardiac cycle at which the stimulus was given. Identical stimuli produced a greater inotropic depression at longer cardiac cycle durations. The differences between the magnitudes of the AP responses at two different mean heart rates were relatively constant when the stimulus intensity was increased from 1 to 99 stimuli per burst. A low-level stimulus caused a greater depression in contractile force when the heart rate was allowed to change than with the identical stimulus when the heart was paced, although this difference in paced- and unpaced-heart responses tended to disappear when the stimulus intensity (burst width) was increased. The composite frequency-force relationship was relatively flat over normal heart periods, but fell off by 15-20% at the extremes. The present results show an unexpected interaction between the vagal inotropic effect and the frequency-force relationship such that a given vagal stimulus causes a greater depression of atrial contractile force at lower heart rates.

Animals↗

Statistical analysis of predominantly transient protein-protein interfaces.

A non-redundant set of 170 protein-protein interfaces of known structure was statistically analyzed for residue and secondary-structure compositions, pairing preferences and side-chain-backbone interaction frequencies. By focussing mainly on transient protein-protein interfaces, the results underline previous findings for protein-protein interfaces but also show some new interesting aspects of transient interfaces. The residue compositions at interfaces found in this study correlate well with the results of other studies. On average, contacts between pairs of hydrophobic and polar residues were unfavorable, and the charged residues tended to pair subject to charge complementarity. Secondary structure composition analysis shows that neither helices nor beta-sheets are dominantly populated at interfaces. Analyzing the pairing preferences of the secondary structure elements revealed a higher affinity within the same elements and alludes to tight packings. In addition, the results for the side-chain and backbone interaction frequencies, which were measured under more stringent conditions, showed a high occurrence of side-chain-backbone interactions. Taking a closer look at the helix and beta-sheet binding frequencies for a given side-chain and backbone interaction underlined the relevance of tight packings. The polarity of interfaces increased with decreasing interface size. These types of information may be useful for scoring complexes in protein-protein docking studies or for prediction of protein-protein interfaces from the sequences alone.

Amino Acids↗

Circadian and hypothermia-induced effects on visual and auditory evoked potentials in multiple sclerosis.

OBJECTIVES: Body cooling has been proposed as a symptomatic treatment for multiple sclerosis. This study aimed to assess the effects of body cooling and of circadian variations on clinical parameters and on visual and auditory evoked potential measures in multiple sclerosis patients. METHODS: Clinical status was assessed and VEPs, BAEPs and MLAEPs (all with two stimulus frequencies) were recorded a total of 4 times on two separate days (two times per day at 08:30 and 15:00 h each day) in 10 multiple sclerosis patients and 10 controls. On one of these days, the subjects were submitted to body cooling before the afternoon session. RESULTS: Tympanic temperature was significantly higher in the afternoon. Cooling lowered the temperature by 1.4 degrees C. No clinical effects were observed. Circadian effects were detected on VEP amplitude, which increased both in controls and in patients at low stimulus frequency (P<0.01), and increased in controls and decreased in patients at high stimulus frequency (interaction: P<0.01). Cooling determined an increase in BAEP I-V peak-to-peak time in controls, and a reduction in patients at high stimulus frequency (interaction: P<0.01). In patients, cooling also determined a great increase in MLAEP amplitude (interaction: P<0.001). We did not find cooling effects on VEP measures. CONCLUSIONS: Visual and auditory evoked potentials showed differences in circadian and cooling effects between controls and multiple sclerosis patients. These differences are consistent with the hypothesis of temperature-dependent conduction blocks in demyelinated fibers. Cooling may have a clinical effect in selected patients only.

Acoustic Stimulation↗

Differential effects of potassium channel blockers on neurohypophysial release of oxytocin and vasopressin. Evidence for frequency-dependent interaction with the endogenous opioid inhibition of oxytocin release.

Isolated rat neurohypophyses were fixed by their stalks to a platinum wire electrode and superfused with Krebs-HEPES solution. Vasopressin and oxytocin released into the medium were determined by specific radioimmunoassays. Hormone secretion was increased by electrical stimulation of the pituitary stalk at different frequencies. The effects of several potassium channel blockers, tetraethyl-ammonium (TEA) ions, 4-aminopyridine (4-AP) and 3,4-diaminopyridine (3,4-DAP) were tested. The release of vasopressin and oxytocin evoked by electrical stimulation with 900 pulses at 15 Hz (about 900 and 1,000 microU, respectively) was about 10 times higher than that evoked by 900 pulses at 3 Hz. Both 10 and 30 mmol/l TEA enhanced the release of vasopressin evoked by stimulation at 3 and 15 Hz, by 25- and 2-fold, respectively, to attain a maximum release of about 1,800 microU per stimulation. The stimulated release of oxytocin attained a maximum of about 9,000 microU at 15 Hz in the presence of 10 mmol/l TEA or at 3 Hz with 30 mmol/l TEA. Thus, in the presence of maximally effective concentrations of TEA both stimulation frequencies (3 and 15 Hz) were equieffective in evoking release of vasopressin and oxytocin. 4-AP or 3,4-DAP enhanced the release of vasopressin evoked by 15 Hz stimulation maximally to about 1,600 microU. In the presence of 4-AP or 3,4-DAP the release of oxytocin evoked by stimulation at 15 Hz increased maximally to about 8,000 microU and that evoked by stimulation at 3 Hz to about 1,500 microU.(ABSTRACT TRUNCATED AT 250 WORDS)

4-Aminopyridine↗

Estimation of peptide hormone noncovalent binding site functionality from frequencies of interaction of amino acid side chains in proteins.

A method is described for estimating the amino acid side chain functional groups most likely to occur at a receptor site (or other protein-type binding site) given a particular peptide hormone sequence. The predictions are based on mutual affinities of amino acid side chains, as manifested in the frequencies of side chain contacts occurring in proteins. The relative merits of various measures of these affinities are discussed, and a new set of contact frequency data based on 36 proteins (coordinate data taken from the Brookhaven Protein Data Bank) is presented. Evaluation of predictions was carried out using subunit-subunit interfaces as models for peptide-receptor association; accuracy of guesses was found to be 3-4 times better than random, according to this measure. Computer programs for carrying out all requisite data manipulations are described.

Amino Acids↗

Frequency-dependent interactions of mexiletine and quinidine on depolarization and repolarization in canine Purkinje fibers.

The use of the antiarrhythmic agents mexiletine and quinidine in combination can be clinically beneficial when single agent therapy is ineffective in ventricular tachyarrhythmias. We therefore compared the effects of mexiletine (10 microM), quinidine (10 microM) and a series of their combinations (total concentration 10 microM) in canine cardiac Purkinje fibers driven at a wide range of stimulation frequencies. All treatments depressed Vmax in tissues driven rapidly (cycle length 300 msec), but only with greater than or equal to 5 microM quinidine was Vmax depressed at longer cycle lengths. Moreover, the time constants for development of and recovery from frequency-dependent block were different: short (200-500 msec) for mexiletine, long (3-5 sec) for quinidine and intermediate or biexponential for the combination. Although both drugs depressed Vmax (albeit with different time dependencies), action potential duration at all cycle lengths from 300 to 8000 msec was shortened by mexiletine, lengthened by quinidine and largely unaltered by combinations. Fibers treated with quinidine in low Ko and driven at slow rates developed marked action potential prolongation and abnormal automaticity in the form of early afterdepolarizations; the addition of mexiletine was sufficient to reverse this effect. In summary, mexiletine and quinidine produced different and frequency-dependent effects on depolarization and repolarization. The effects of their combinations reflected these actions, with opposing actions on repolarization at any stimulation rate but additive frequency-dependent depression of Vmax. We conclude that the clinically beneficial effects of this combination may reflect additive frequency-dependent effects on cardiac sodium channels in the face of unaltered repolarization time.

Action Potentials↗

Complex frequency-dependent interaction of class-I antiarrhythmic drugs as they affect intraventricular conduction.

We investigated the interaction of class-I antiarrhythmic drugs as they affect intraventricular conduction of human hearts in vivo. QRS duration in signal-averaged electrocardiograms and standard electrocardiograms were measured as an index of intraventricular conduction time in 17 patients with implanted pacemakers at various pacing rates (100-180 ppm, VVI mode). Single intravenous administration of lidocaine, disopyramide or aprindine prolonged the QRS of signal-averaged electrocardiograms in a frequency-dependent manner. Lidocaine (n = 17) produced significant QRS prolongation from pre-drug control at rates > or = 120 ppm (6.2 +/- 1.4% at 180 ppm), whereas disopyramide (n = 17) and aprindine (n = 17) did so from the lowest rate (8.9 +/- 1.8% to 12.3 +/- 2.9% at 100-180 ppm with disopyramide; 14.7 +/- 1.3% to 19.3 +/- 2.2% at 100-180 ppm with aprindine). Addition of lidocaine to disopyramide (n = 17) showed an additive effect; QRS prolongation was enhanced significantly by 1.4-2.8% at rates > or = 150 ppm. In contrast, addition of lidocaine to aprindine (n = 17) showed a subtractive effect; the QRS prolongation was attenuated significantly by 1.6-2.4% at rates < 150 ppm. Combined intravenous administration of class-I antiarrhythmic drugs causes not only additive but also subtractive effects on the intraventricular conduction of the human heart, probably through their interaction on the sodium channel receptor.

Aged↗