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An immunohistochemical study of the behavior of cells expressing interleukin-1 alpha and interleukin-1 beta within experimentally induced periapical lesions in rats.

Cells expressing interleukin-1 (IL-1) were examined in rat periapical lesions using immunohistochemical methods. During the acute phase, both IL-1 alpha- and IL-1 beta-expressing cells were observed adjacent to many osteoclasts that contained large cytoplasms, and consequently the periapical lesions tended to expand. However, the number of IL-1 beta-expressing cells was about twice that of IL-1 alpha-expressing cells during the same period. On the other hand, there were significantly less IL-1 beta-expressing cells during the chronic phase and consequently a significant expansion of the periapical lesions was not seen. Furthermore, IL-1 alpha- and IL-1 beta-expressing cells were located far from the bone surfaces, whereas a fibroblastic cell layer lay between the IL-1-expressing cells and the bone surfaces. Both IL-1 alpha and IL-1 beta were mainly expressed by macrophages. In addition, after the percolated application of formocresol to experimentally induced periapical lesions, numerous macrophages expressing IL-1 beta became conspicuous within the vicinity of the extensive bone resorption sites. These results suggest that macrophages expressing IL-1 beta may play an important role in the activation and recrudescence of osteoclastic bone resorption, and this may be due to their localization close to the bone resorption sites noted within experimental periapical lesions.

Alveolar Bone Loss↗

Acute care of severe tooth fractures.

Teeth that are severely fractured and demonstrate vital tissue at the fracture line may be treated acutely by extirpating the pulp. This technique leaves the patient free of pain until secondary care is available.

Anesthesia, Dental↗

Ultrasonic root-end preparation: clinical and radiographic evaluation of results.

PURPOSE: This study evaluated the results of periradicular surgery performed using ultrasonic root-end preparation. PATIENTS AND METHODS: The course of healing during observation periods ranging from 6 months to 3 years was examined in 157 teeth. RESULTS: One hundred forty-five teeth were successfully treated, for a success rate of 92.4%. CONCLUSION: It was concluded that the ultrasonic root-end preparation technique for periradicular surgery is a simple procedure that provides excellent results.

Adolescent↗

The early effects of segmental surgery on the human pulp.

The teeth of two patients requiring preprosthetic segmental surgery were removed between 4 and 10 weeks postoperatively. The pulps of these teeth were examined histologically using sound teeth from patients of the same age as controls and it was generally found that the teeth from the repositioned segment retained their vitality; there was progressive degeneration of nerves except for the nonmyelinated nerves of the autonomac nervous system, which remained intact.

Adolescent↗

Histologic analysis of pulpal revascularization of autotransplanted immature teeth after removal of the original pulp tissue.

The survival rate of replanted and autotransplanted teeth is mainly affected by the reaction of the pulp. Pulpal necrosis can cause periapical inflammation and inflammatory root resorption. The purpose of this study is to learn more about the pulpal changes in autotransplanted immature teeth whose pulp tissue was removed before transplantation. The experimental material consisted of 16 single-rooted teeth with open apices, from a beagle dog (3 months of age). At day 0, 4 teeth were extracted, the pulpal tissues were removed, and the teeth were then transplanted to their contralateral side. The same procedure was carried out on days 9, 16, and 23, each time for 4 single-rooted teeth. Longitudinal paraffin sections were made for histologic investigation. The results showed that, after 7 days, 2 of the 4 teeth had an ingrowth of new tissue over one fourth of their length. After 14 days, all 4 teeth had ingrowth (> or =one fourth of the pulp chamber). At the 21-day observation, more than half of the pulp chambers of all teeth were filled, and, after 30 days, there was total ingrowth in 3 of the 4 teeth. This new tissue consisted of well-organized and well-vascularized connective tissue.

Animals↗