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In vitro evaluation of the reliability of the Root ZX electronic apex locator.

AIM: An experimental comparison of the Root ZX apex locator with the real measurement of the root canal length was carried out using a saline gel to simulate the periodontium. METHODOLOGY: Lengths were taken when the needle reached the 0.5 mark and the Apex mark on the meter. These measurements were then compared with a calculated reference length representing the real length of the root canal. Reliability was assessed using Student's t-test. Precision was assessed using the mean and standard deviation of differences. RESULTS: The Root ZX gave measurements within a range of 0.5 mm in 84.72% of the cases. The intra-operator and inter-operator variabilities were not statistically significant (P > 0.05). CONCLUSIONS: The Root ZX is not capable of detecting the '0.5 mm from the foramen' position and thus, should only be used to detect the foramen (major diameter).

Dental Instruments↗

Root-canal preparation with FlexMaster: canal shapes analysed by micro-computed tomography.

AIM: To evaluate the relative performance of FlexMaster nickel-titanium instruments shaping maxillary molar root canals in vitro. METHODOLOGY: Extracted human maxillary molars were scanned, before and after root-canal shaping, with FlexMaster, employing micro-computed tomography (microCT) at a resolution of 36 microm. Canals were three-dimensionally reconstructed and evaluated for volume, surface area, 'thickness' (diameter), canal transportation and prepared surface. Based on median canal volume, the canals were divided into 'wide' and 'constricted' groups. Comparisons were made between mesiobuccal (mb), distobuccal (db) and palatal (p), as well as 'wide' and 'constricted' canals, using anova and Scheffé post-hoc tests. RESULTS: Volume and surface area increased significantly and similarly in mb, db and p canals, and no gross preparation errors were found. Mean root-canal diameters, 5 mm coronal to the apex, increased from 0.45 to 0.65 mm, from 0.41 to 0.56 mm and from 0.79 to 0.85 mm for mb, db and p canals, respectively. Apical canal transportation ranged from 0.01 to 0.29 mm and was independent of canal type; 'wide' canals had a significantly higher (P < 0.05) proportion of unprepared surfaces than 'constricted' canals had. CONCLUSIONS: FlexMaster instruments shaped curved and narrow root canals in maxillary molars to sizes 40 and 45 without significant shaping errors. Canal anatomy had an insignificant impact on preparation, indicating that FlexMaster instruments were able to shape 'constricted' canals as well as 'wide' ones.

Analysis of Variance↗

The effects of file size, sodium hypochlorite and blood on the accuracy of Root ZX apex locator in enlarged root canals: an in vitro study.

BACKGROUND: The initial electronic apex locator (EAL) length measurement is generally established with a small-sized file. It is not known whether file size would be interfering with the reading accuracy of the EAL. This study aimed to evaluate the effect of file size on the accuracy of Root ZX apex locator using an agar model when sodium hypochlorite solution or blood was present during electronic measurements in enlarged root canals. METHODS: A total of 36 extracted lower premolars were used. In stage 1, the canals were instrumented using size 10-40 K-files with a size 40 K-file as the master apical file (MAF). The teeth were then divided randomly into two groups of 18 teeth each. In group A, the teeth were mounted in one per cent agar and irrigated with six per cent sodium hypochlorite solution (NaOCl), while in group B the teeth were mounted in agar and irrigated with human blood. In stage 2, the canals were enlarged using a size 60 K-file as the MAF. In stages 1 and 2, the apical portions of the canals were instrumented using the step-back sequence (up to a size 80 K-file). In stage 3, the canals were enlarged using a size 80 K-file as the MAF. In each stage, the length was measured with a Root ZX until the meter value reached 'APEX' using small and large size files. RESULTS: Three-way ANOVA and Bonferroni test showed that file size, stage of preparation and type of irrigant all had a significant influence on the measurement error (P < 0.0001), with all the interactions between these three factors being significant (P < 0.0001). CONCLUSIONS: As the diameter of the root canal increased, the measured length with the smaller size files became shorter. A file of a size close to the prepared canal diameter should be used for root length measurement in the presence of blood. In the presence of NaOCl, the Root ZX was highly accurate even when the file was much smaller than the diameter of the canal. The agar model was effective and suitable for testing EALs in vitro.

Analysis of Variance↗

Three phosphor plate systems and film compared for imaging root canals.

AIM: The aim of this study was to compare three phosphor plate intraoral imaging systems (Digora (DA), Digident (DT), Denoptix (DX)) and E-speed conventional film (CF) for the imaging of root canals. METHODOLOGY: Sixty extracted permanent teeth were exposed using CF, DA, DT and DX. The length of root canal visible from its most apical extent to a line drawn at the level of the cementoenamel junction was measured and this was expressed as a percentage of the distance between the radiographic apex and the cementoenamel line. All images were examined concurrently by two examiners. Films were viewed under standardized conditions and DA, DT and DX images were viewed directly from the monitor screen. The images on the monitor were enhanced to give the subjectively clearest image. RESULTS: The mean percentage of canal visible on CF was 90%, DA 78%, DT 81% and DX 83%. The difference was significant for CF-DA (P < 0.001) and CF-DT (P = 0.001). There was no statistically significant difference for CF-DX or between any of the three phosphor plate imaging systems. CONCLUSIONS: It is concluded that a greater length of root canal was visible on conventional film than on three phosphor plate imaging systems and that this may be of clinical significance.

Analysis of Variance↗

The location of the cementodentinal junction in single-rooted mandibular first premolars from Egyptian and Saudi patients: a histological study.

AIM: To determine the location of the cementodentinal junction (CDJ) in mandibular first premolars from Egyptian and Saudi patients. METHODOLOGY: One hundred and twenty-two extracted single-rooted mandibular first premolars (68 Egyptian; 54 Saudi) were examined histologically. Half of the teeth were fixed, decalcified, embedded in paraffin, mesio-distally sectioned and stained with H&E, whilst the other half were ground with a stone prior to microscopic evaluation. Measurements of the location of the CDJ were made parallel to the long axis of the teeth from the external surface of the root apex on the mesial and distal sides of the canal. Measurements were statistically analysed using two-way anova of repeated-measure design. RESULTS: The CDJ in Egyptian sections ranged from 0 to 2.0 mm (mean +/- SD = 0.9 +/- 0.4 mm) on the mesial side of the canal and from 0.2 to 2.5 mm (mean +/- SD = 1.0 +/- 0.5 mm) on the distal aspect. In the Saudi sample, the CDJ ranged from 0.5 to 1.2 mm (mean +/- SD = 0.8 +/- 0.3 mm) on the mesial side and from 0.6 to 0.9 mm (mean +/- SD = 0.7 +/- 0.4 mm) on the distal side. The mesial and distal differences between Egyptian and Saudi premolars on the mesial and distal surface were statistically significant (P < 0.001). CONCLUSIONS: The location of the CDJ varied between the Egyptian and Saudi mandibular first premolars, indicating that this junction is not a fixed point in populations of different countries.

Adolescent↗

A comparison of dentine removal using safety or conventional Hedstrom files.

Mandibular molars (n = 20) were divided and the mesial roots mounted in an acrylic mould which allowed them to be removed, sectioned horizontally and then reassembled. The furcal root canal wall thickness 7 mm from the apex was measured prior to instrumentation. The roots were allocated to two groups (n = 10). One group was instrumented using the step back technique with safety Hedstroms in one canal and conventional Hedstrom files in the other canal. The second group was instrumented using a crown down approach (using files and Gates Glidden drills to provide early radicular access). The furcal root canal wall thickness was then remeasured and the amount of dentine removed calculated. The results showed that the safety Hedstrom file removed less dentine from the furcal region of the canal compared to the conventional Hedstrom file. This however was not statistically significant (Paired t test, n = 10, p > 0.05). The amount of dentine removed using the crown down technique was significantly greater than that using the step back technique (Two sample t, n = 20, p < 0.01). In conclusion, less dentine was removed from the furcal region with safety Hedstrom files compared to conventional Hedstrom files; however, Gates Glidden drills removed more dentine than either type of hard file.

Dental Instruments↗

An in vitro study of apical and coronal microleakage of laterally condensed gutta percha with Ketac-Endo and AH-26.

The purpose of this in vitro study was to compare both apical and coronal dye penetration when Ketac-Endo and AH-26 sealers were used with laterally condensed gutta percha. Crowns were removed from 28 teeth and the root canals were biomechanically prepared. The teeth were divided into two groups of 12-teeth each and a control group of 4 teeth. Root canals in the two experimental groups were filled with laterally condensed gutta percha and either Ketac-Endo or AH-26 sealer. The Ketac-Endo group had the coronal 3 mm of gutta percha and sealer removed and the resultant cavity was filled with Ketac-Endo alone. After the sealers had set, the root surfaces were coated with nail varnish except at the apex and at the coronal end. Positive controls had no root fillings and were coated with nail varnish in the same manner while the negative controls were sealed apically and coronally with Cavit prior to sealing the entire external root surface with nail varnish. Specimens were placed in 2% methylene blue dye in a vacuum of 660 mm of mercury for five minutes and then left immersed for a further two days. The roots were vertically sectioned to determine the following mean levels of dye penetration: Ketac-Endo, 1.08 mm apically and 6.29 mm coronally; AH-26, 0.75 mm apically and 6.67 mm coronally. Positive controls had total leakage and negative controls had no leakage. This study demonstrated that the apical and coronal seals obtained with Ketac-Endo and AH-26 were not significantly different although the apical seal obtained with each material was significantly better than the corresponding coronal seal.

Bismuth↗

Accuracy of the Justy II Apex locator in determining working length in simulated horizontal and vertical fractures.

AIM: To study the effectiveness of an electronic apex locator (EAL; Justy II; Yoshida Dentcraft, Tokyo, Japan) in locating simulated horizontal and vertical fractures in single roots. METHODOLOGY: An EAL was used to measure the distance within the canal of horizontal (n = 31) and vertical (n = 31) fractures, created with a disk in single-rooted teeth. Accuracy of the EAL was evaluated by comparing the measurements with those made using a size 10 file. Data were analysed with the non-parametric Passing and Bablok method. RESULTS: For simulated horizontal fractures, the EAL measured exactly the same length as a size 10 file, without constant or proportional errors. In vertical simulated fractures, the EAL measured (on average) with a constant error of 7.5 mm shorter than the size 10 file; the difference had a wide confidence interval (-72.3 to 2.6 mm). CONCLUSIONS: In this laboratory study, the Justy II EAL was able to determine accurately the position of simulated horizontal fractures, but was unreliable when measuring simulated vertical fractures.

Dental Instruments↗

A comparison of thermocouple and infrared thermographic analysis of temperature rise on the root surface during the continuous wave of condensation technique.

AIM: This study was designed to use two methods of temperature measurement to analyse and quantify the in vitro root surface temperature changes during the initial stage of the continuous wave technique of obturation of 17 single-rooted premolar teeth with standard canal preparations. METHODOLOGY: A model was designed to allow simultaneous temperature measurement with both thermocouples and an infrared thermal imaging system. Two thermocouples were placed on the root surface, one coronally and the other near the root apex. A series of thermal images were recorded by an infrared thermal imaging camera during the downpack procedure. RESULTS: The mean temperature rises on the root surface, as measured by the two thermocouples, averaged 13.9 degrees C over the period of study, whilst the infrared thermal imaging system measured an average rise of 28.4 degrees C at the same sites. Temperatures at the more apical point were higher than those measured coronally. After the first wave of condensation, the second activation of the plugger in the canal prior to its removal always resulted in a secondary rise in temperature. The thermal imaging system detected areas of greater temperature change distant from the two selected thermocouple sites. CONCLUSIONS: The continuous wave technique of obturation may result in high temperatures on the external root surface. Infrared thermography is a useful device for mapping patterns of temperature change over a large area.

Adult↗

Microbial leakage of Cavit, IRM, and Temp Bond in post-prepared root canals using two methods of gutta-percha removal: an in vitro study.

The aim of this study was to evaluate the integrity of the coronal seal of Temp-Bond and compare it to Cavit and IRM after post space preparation using S. faecalis as a microbial tracer. In addition, the affect of two methods of gutta percha removal on the apical seal of root canal fillings was also evaluated. Forty extracted human single rooted teeth were prepared chemomechanically and obturated with gutta percha and AH26 sealer cement using the lateral cold condensation technique to a standardized working length of 15 mm. About 10 mm of the coronal gutta-percha was removed with either Peeso-reamer or a hot plugger. The roots were divided into three experimental groups of 10 roots and a control group. Each experimental group was subdivided equally into two groups of 15 each according to the method of post space preparation. Cavit, IRM, and Temp-Bond were used to seal the access opening. Each root was fixed in a cuvette containing Tryptic Soya Broth which, covered 2 mm of the root apex. Bacterial suspension was introduced through pipette. Fresh bacterial suspension was added every week, and the system was monitored daily for the growth of microorganisms for a period of one month. The results showed there was no significant difference in terms of coronal leakage between the three coronal materials used (P=0.478), but the methods of gutta-percha removal did have an impact on the apical leakage (P=0.047). The mean value showed the Peeso-reamer provided less leakage compared to using a hot plugger during the 30-day experimental time period. It was concluded the temporary type of coronal seal of endodontically treated teeth will not prevent coronal leakage if left for a long period of time. In addition, permanent cementation of the post with the coronal restoration should be carried out as soon as possible to prevent recontamination of the root canal.

Analysis of Variance↗

Clinical accuracy of a new apex locator with an automatic compensation circuit.

A new circuit was designed to automatically compensate for measurement errors of an electronic apex locator in various electrolytes. Thirty-one root canals were clinically tested for accuracy. A file was inserted into the canal until the apex signal was obtained, at which point the file was immobilized with glass-ionomer cement. After extraction, the apical area was exposed and the position of the file tip was examined under an operating microscope. Distances from the major foramen and cemento dentinal junction (CDJ) were recorded. The average distance from the major foramen was -0.13 mm with a range of -1.28 mm and +0.46 mm. The average distance in 26 detectable CDJ samples was +0.18 mm with a range of -0.98 mm and +0.65 mm. The measurements, which were within +/- 0.5 mm, were 94% (29/31) from the major foramen and 92% (24/26) from the CDJ. Measurement consistencies within one SD were 81% for the major foramen and 65% for the CDJ, respectively. Measurements within two SD were 97% for the major foramen and 92% for the CDJ. There were no differences between the smaller (< # 25) and larger apical foramens (< or = 25) or vital and nonvital pulps.

Adult↗

Electronic length measurement using small and large files in enlarged canals.

Electronic apex locators are frequently used attached to a small size endodontic file; however, the effect on the measurement of the relative diameters of the file and the root canal has not been clarified. In this study, the length of enlarged canals was measured using small size files and files matching the canal diameter to observe a possible discrepancy. The accuracy of electronic length control during canal preparation with rotary files was also assessed. The root canals in 21 extracted, single rooted teeth were accessed, and their actual length (AL) established by passing a size 10 file just through the minor apical foramen. The teeth were then embedded in an alginate mold. The initial canal length (IL) was measured with the Root ZX apex locator by negotiating a size 10 file to the apical constriction. The canal was enlarged to size 60 with rotary files while the length was continuously controlled with the apex locator. Then, the final length measurements were obtained with a size 10 file and a size 60 file (FL-10 and FL-60, respectively). The average values of IL, FL-10 and FL-60 were calculated and compared using Repeated Measures Analysis of Variance followed by Tukey's Studentized Range test (P < 0.05). Using composite resin, the size 60 files were secured at the FL-60 length, the teeth were removed from the alginate mold, stained with Picroformal DI Buoin stain and the position of the file tip was observed histomorphometrically after the apical 4 mm of the canal was exposed by grinding the buccal aspect of the root. The IL was 0.45 mm shorter than AL (P < 0.05). The differences between FL-10, FL-60 and IL were not statistically significant. Histomorphometrically, the apical constriction was absent in all the teeth, but the file tips were confined within the root. This study concluded that the Root ZX indicated the location of an apical constriction even when the anatomic constriction was eliminated. In the enlarged canals, length measurements obtained with small and large size files were comparable.

Analysis of Variance↗

Evaluation of ultrasonically placed MTA and fracture resistance with intracanal composite resin in a model of apexification.

The purpose of this study was to evaluate whether intracoronal delivery of an apical barrier of mineral trioxide aggregate (MTA) placed ultrasonically, non-ultrasonically, or ultrasonically with the addition of an intracanal composite resin provided a better seal against bacterial leakage. A second purpose was to determine whether intracanal composite resin or gutta-percha and sealer placed against an apical barrier of MTA provided greater resistance to root fracture. In a standardized in vitro open apex model, MTA was placed as an apical barrier at a thickness of 4 mm, with and without ultrasonic vibration. The barriers were challenged with bacteria exposure within a leakage model, and fracture resistance was assessed with increasing forces applied via an Instron machine. After 45 days, the addition of ultrasonics significantly improved the MTA seal, compared with the non-ultrasonics treatment (Kruskal Wallis nonparametric ANOVA with Dunn multiple comparison test p < 0.05). Bacterial leakage occurred in 6 (33%) of 18 in the non-ultrasonic MTA group, 2 (11%) of 18 in the ultrasonic MTA group, and 1 (6%) of 18 in the ultrasonic MTA-composite group. There were no significant differences at 90 days. A 4-mm thickness of MTA followed with an intracanal composite resin demonstrated a significantly greater resistance to root fracture than MTA followed with gutta-percha and sealer (one-way ANOVA with Newman-Keuls multiple comparison test, p < 0.01). The MTA-gutta-percha group was not significantly different than the MTA unrestored positive control.

Aluminum Compounds↗

Residual dentin thickness in mesial roots of mandibular molars prepared with Lightspeed rotary instruments and Gates-Glidden reamers.

OBJECTIVE: We sought to measure the residual dentin thickness (RDT) in the mesial roots of mandibular molars after instrumentation with Lightspeed and Gates-Glidden rotary instruments. Study design Thirty extracted, untreated human mesial roots of mandibular molars were separated from the distal roots and embedded in clear polyester resin. The roots were cut horizontally at 1, 4, and 7 mm short of the anatomic apex. The diameter of each mesiobuccal canal was measured by using a stereo measuring microscope at each level in the buccolingual and mesiodistal directions. The dentin thickness was measured in each level in the mesial, distal, buccal, and lingual directions. Sections were reassembled with a muffle. The canals were enlarged to the working length with Lightspeed rotary instruments, of which the average size used was a No. 50 file. The coronal third was flared with No. 2 Gates-Glidden reamers. Slices were separated again, and the RDT and canal diameters were measured. RESULTS: The minimal measured RDT after instrumentation at the 1-, 4-, and 7-mm levels was 0.70 +/- 0.28 mm, 1.04 +/- 0.18 mm, and 1.09 +/- 0.19 mm, respectively. The average diameter of the canals after instrumentation at the 1-, 4-, and 7-mm levels was 0.50 +/- 0.04 mm, 0.52 +/- 0.05 mm, and 0.74 +/- 0.08 mm, respectively. The canal diameter did not exceed one third of the root diameter at all levels. CONCLUSIONS: Root canal preparation of mandibular mesial roots with Lightspeed instruments to No. 50 in the apical third and Gates-Glidden reamers to No. 2 in the coronal third does not significantly decrease the RDT.

Dental Pulp Cavity↗

Prognostic implications of nasal cavity and cleft morphology in secondary bone grafting.

OBJECTIVE: To examine the prognostic significance of the skeletal morphology around the nasal cavity and the alveolar cleft in secondary bone grafting (SBG). DESIGN AND SETTING: Fifty-one alveolar clefts in 41 patients (10 bilateral and 31 unilateral cleft lips and palates) registered in the Tokushima University Dental Hospital were examined in this study. METHOD: Evaluation of the bony bridge after SBG using dental radiographs at 1 year after surgery. The clefts were divided into two groups: group I (54.9%) in which the upper border of the bony bridge was preferably maintained on or above the horizontal reference line (RL) constructed at the level of the root apex of the upper central incisor adjacent to the cleft, and group II (45.1%) in which the bone level was lower than the RL. Presurgical cleft width was determined by the dental radiographs. The cleft/nasal cavity ratio; the value of the cleft width divided by the nasal cavity width on the cleft side, which was analyzed by frontal cephalograms before the SBG; and the cleft/apertura piriformis ratio, the value analyzed by computed tomography, were used. RESULTS AND CONCLUSION: The age, sex, and eruptive stage of the canine teeth at the time of the SBG showed no significant difference between groups. The presurgical cleft width also showed no significant difference between group I (6.6 +/- 3.1 mm) and group II (7.9 +/- 3.3 mm). The cleft/nasal cavity ratio showed a significant difference between groups I and II (0.42 +/- 0.14, 0.75 +/- 0.25; p < .05). Furthermore, the cleft/apertura piriformis ratio also showed a significant difference between groups I and II (0.32 +/- 0.12, 0.65 +/- 0.26; p < .05). These results suggested that measurements of the skeletal morphology around the nasal cavity and alveolar cleft might aid in predicting the stability of the bony bridge after SBG.

Adolescent↗

Influence of cervical preflaring on determination of apical file size in maxillary premolars: SEM analysis.

The purpose of this study was to investigate the influence of cervical preflaring on the determination of the first file that binds at working length (WL) in buccal roots of maxillary premolars. Five groups (n=10) were formed at random and, after standard access cavities, the WL was determined 1 mm short from the apex. In group 1, the initial apical file was inserted without preflaring of cervical and middle thirds of the root canals. In groups 2 to 5, the cervical and middle thirds were enlarged with sizes 90 and 110 Gates-Glidden drills, K(3) Orifice Opener instruments, ProTaper instruments and LA Axxess burs, respectively. Canals were sized manually with K-files, starting with No. 08 K-files inserted passively up to the WL. File sizes were increased until a binding sensation was felt at the WL and the size of the instrument was recorded. Transversal sections of the WL regions were examined under scanning electron microscopy and the discrepancies between the canal diameter and first file to bind at the WL were assessed. Significant differences (p<0.001) were found between the groups. The major discrepancy was found without preflaring (mean 157.8 microm). LA Axxess burs produced the smallest discrepancy (mean 0.8 microm). Gates-Glidden drills and K(3) Orifice Opener instruments showed no significant differences (p>0.05) between their results (83.2 microm and 73.6 microm, respectively). The discrepancy for ProTaper instruments was 35.4 microm on average. In conclusion, the instrument binding technique for determination of the anatomical diameter at the WL was not precise. Preflaring of the cervical and middle thirds improved the determination of the anatomical diameter at the WL, and the type of instrument played a major role. Canals preflared with LA Axxess burs showed a more accurate binding of the files to anatomical diameter.

Analysis of Variance↗

Standing oral extraction of cheek teeth in 100 horses (1998--2003).

REASONS FOR PERFORMING STUDY: Extraction of cheek teeth (CT) by the conventional repulsion technique requires general anaesthesia and carries a high rate of post operative complications. Consequently, an alternative method of extraction, i.e. orally in standing horses, was evaluated. HYPOTHESIS: The need for and risks of general anaesthesia could be avoided and post extraction sequelae reduced by performing extractions orally in standing horses. METHODS: One hundred mainly younger horses (median age 8, range 2-18 years) with firmly attached CT that required extraction because of apical infections, displacements, diastemata, idiopathic fractures and the presence of supernumerary CT had the affected teeth (n = 111) extracted orally under standing sedation. Follow-up information was obtained for all cases, a median of 16 months later. RESULTS: Oral extraction was successful in 89 horses and unsuccessful in 11 due to damage to the CT clinical crown (n = 9) during extraction, for behavioural reasons (n = 1) and because the apex of a partly extracted CT fell back into the alveolus following sectioning (n = 1). Predispositions to extraction-related CT fractures were present in 5 of the 9 cases, i.e. advanced dental caries (n = 2) and pre-existing 'idiopathic' fractures (n = 3). The iatrogenically fractured CT were later repulsed under standing sedation (n = 3) and under general anaesthesia (n = 6). Eighty-one of the remaining 89 horses had successful oral CT extraction with no or minimal intra- or post operative complications occurring. Post operative complications in the other 8 cases included post extraction alveolar sequestration (n = 3), alveolar sequestration and localised osteomyelitis (n = 1), localised osteomyelitis (n = 1), incorporation of alveolar packing material into alveolar granulation tissue (n = 1), and nasal discharge due to continued intranasal presence of purulent food material (n = 1) and to ongoing sinusitis (n = 1). The above sequelae were treated successfully in all cases, with general anaesthesia required in just one case. Following oral extraction, significantly (P<0.001) fewer post operative problems developed in 54 horses with apically infected CT in comparison with 71 previous cases that had repulsion of apically infected CT at our clinic. CONCLUSIONS AND POTENTIAL RELEVANCE: Oral extraction of cheek teeth is a successful technique in the majority of younger horses with firmly attached CT and greatly reduces the post operative sequelae, compared with CT repulsion. Additionally, the costs and risks of general anaesthesia are avoided. Further experience and refinement in the described protocol could potentially increase the success of this procedure and also reduce the incidence of post operative sequelae.

Analgesics, Opioid↗

The effect of mineral trioxide aggregate on the apexification and periapical healing of teeth with incomplete root formation.

AIM: To evaluate the influence of mineral trioxide aggregate (MTA) on apexification and periapical healing of teeth in dogs with incomplete root formation and previously contaminated canals and to verify the necessity of employing calcium hydroxide paste before using MTA. METHODOLOGY: Twenty premolars from two 6-month old dogs were used. After access to the root canals and complete removal of the pulp, the canal systems remained exposed to the oral environment for 2 weeks. Canal preparation was then carried out using Hedström files, under irrigation with 1% sodium hypochlorite, 1 mm short of the radiographic apex. After drying, the canals of two premolars in each dog were left empty (control group). The other eight teeth in each animal were divided into two experimental groups. The apical thirds of the canals of group 1 were filled with MTA. In the teeth of group 2, the canals were dressed with a calcium hydroxide-propylene glycol paste. After 1 week, the paste was removed and the apical third was filled with MTA. All teeth were restored with reinforced zinc oxide cement (IRM) and amalgam. The animals were killed 5 months later, and blocks of the teeth and surrounding tissues were submitted to histological processing. The sections were studied to evaluate seven parameters: formation of an apical calcified tissue barrier, level of barrier formation, inflammatory reaction, bone and root resorption, MTA extrusion, and microorganisms. Results of experimental groups were analysed by Wilcoxon's nonparametric tests and by the test of proportions. The critical value of statistical significance was 5%. RESULTS: Significant differences (P < 0.05) were found in relation to the position of barrier formation and MTA extrusion. The barrier was formed in the interior of the canal in 69.2% of roots from MTA group only. In group 2, it was formed beyond the limits of the canal walls in 75% of the roots. MTA extrusion occurred mainly in roots from group 2. There was similarity between the groups for the other parameters. CONCLUSIONS: Mineral trioxide aggregate used after root canal preparation favoured the occurrence of the apexification and periapical healing. The initial use of calcium hydroxide paste was not necessary for apexification to occur, and has shown to be strongly related to the extrusion of MTA and formation of barriers beyond the limits of the root canal walls.

Aluminum Compounds↗