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PubMed1979

A study on the diagnostic methods for Trichomonas vaginalis infection.

A comparison of the sensitivity of the wet smear, Papanicolaou's stained smear, and culture in Cystein-Peptone-Liver-Maltose (CPLM) medium for the diagnosis of T. vaginalis infection was undertaken in 1, 197 female subjects, both with and without gynaecological disorders. The wet smear method gave 15.5% positives, culture method, 16.5% positives and Papanicolaou's stained smear 6.7% positives. All positive cases by Papanicolaou's method or wet smear method were positive by the culture method. These findings indicate that the wet smear and culture in CPLM medium were better than the Papanicolaou's method.

Female↗

PubMed1982

Epidemiology of sexually transmitted diseases in the Scandinavian countries.

In the Scandinavian countries, laws require reporting of the traditional venereal diseases, i.e., gonorrhea, syphilis, lymphogranuloma venereum (LGV), and ulcus molle, or chancroid. Incidence figures for these diseases, therefore, are fairly reliable. In the Scandinavian countries, LGV and chancroid are rare diseases, and the syphilis incidence has remained low. These three diseases are typically male afflictions. Gonorrhea outnumbers the other diseases mentioned and at present has a nearly 1:1 male: female ratio. In Scandinavia, as in other parts of the world after World War II, gonorrhea has shown great epidemiological fluctuations. A heavy increase in its incidence was evident in Sweden and Denmark during the 1960s. During the 1970s, however, the incidence in Sweden dropped significantly, with the 1980 figures corresponding to those seen in 1960. Sweden now has the lowest incidence of gonorrhea among the Scandinavian countries. During the past three years, the incidence of infections with penicillinase-producing gonococci (PPNG) has increased. Most of the strains isolated have originated from the Far East. As documented in several recent investigations, genital chlamydial infections are at least as common as gonorrhea in the Scandinavian countries. Acute salpingitis has been shown to be a common complication of such infections. Among the other sexually transmitted diseases (STD), Trichomonas infections are still common. The prevalence of genital warts and of Herpes Simplex Virus (type 2) infections is increasing and within the next few years these probably can be expected to become an even greater problem than are the traditional venereal diseases, because no specific treatment is yet available.

Adolescent↗

PubMed1997

A rapid diagnostic test for Trichomonas vaginalis infection.

The applicability of the quantitative buffy coat tube test (QBC) for diagnosis of T. vaginalis was studied. The results of the QBC tube test was compared with that of the wet mount examination in 60 women having genital infection. The cases were selected from outpatient clinic of El-Shatby University Hospital T. vaginalis was identified by QBC tube test in 24 cases out of 60 while the wet mount smear, it was detected in 21 cases. The sensitivity of the QBC tube test was 100% and the specificity was 92.3%. It was found that the QBC tube test seems to have a place in the routine diagnosis of T. vaginalis.

Adult↗

PubMed1977

Single dose of two grams of metronidazole for Trichomonas vaginalis infection.

In this study, 243 women with symptomatic trichomonal vaginitis were treated with eight 250 mg. tablets of metronidazole for themselves, and eight tablets were given to each of their male consorts. They were given instructions to ingest "all eight in a row" and "avoid alcohol for 48 hours." Ninety-five per cent of 203 women that returned for re-examination were cured. This single-dose treatment is effective, well tolerated, and less expensive than current recommended dosages.

Administration, Oral↗

PubMed1990

[Several years of observation of the incidence of Trichomonas vaginalis infections among female workers in a single industrial plant].

During 5 years cycle a prophylactic gynaecological, cytological and parasitological examinations of 1918 women were performed. 12.8% of women were infected with T. vaginalis. Decreasing percentage of infections was observed: from 9.92% in 1984 to 2.2% in 1988. In the I. of Papanicolaou cervical smears T. vaginalis has been found in 1.60% of cases, in the II.--in 5.2%, in the III.--in 14.29%, and in the IV.--in 22.22% of cases. In women with normal cervix uteri T. vaginalis have been found in 5.01% of cases, and in women with hypertrophied cervix and partially epidermized ectopy with ovula nabothi in 15.28% of cases.

Engineering↗

PubMed1990

[Incidence of Trichomonas vaginalis infection among girls from the Lódź population in the last 25-year period].

19,024 girls (aged: from 0 to 18) attending the Pediatric Gynaecology Outpatient Department and the Center for Treatment of Parasitic and Mycotic Diseases were examined. The diagnosis of infection with T. vaginalis was based on preparations using various methods and cultures on specific media, from vaginal content and urine. It was found that the frequency of T. vaginalis invasions in girls had lessened 2-6 times in different groups from 1960 till 1985 and the higher percent of T. vaginalis in girls over 10 years old had been observed than in younger ones. There exists the statistically significant correlation (P less than less than 0.02) between T. vaginalis invasions in girls and other persons from their environment (families).

Adolescent↗

PubMed2001

In-pouch TV culture system in diagnosis of Trichomonas vaginalis infection.

A newly simplified culture method, the In-Pouch TV culture system, the wet mount (WM) examination and the acridine orange stain were compared with the Oxoid culture as a standard technique in the diagnosis of trichomoniasis. Out of 70 symptomatic cases enrolled in this study, 28 specimens were positive by all methods. Among these positive specimens, 21 were positive by the Oxoid culture (75%) and 24 (85.7%) with sensitivity of 85.7% by the In-Pouch system. Both wet mount preparation and acridine orange stain had less sensitivity than the In-Pouch system (61.9%) and detected 15 (53.6%) and 16 (57.1%) of the cases respectively. The In-Pouch system has been proved to be easier in the transport and culture technique than the ordinary culture method. It alleviates the need to enter the culture, thus prevents contamination. Its cost is comparable to the ordinary culture tube. Therefore, it is recommended to use the In-Pouch culture system as a method of diagnosing trichomoniasis.

Acridine Orange↗

PubMed1999

Diagnosis of symptomatic and asymptomatic Trichomonas vaginalis infection by applying one tube nested PCR to vaginal discharge.

One tube nested-PCR targeting a species-specific Tv-E650 repeat family of T. vaginalis genome, was applied to vaginal discharge specimen to diagnose symptomatic and asymptomatic trichomoniasis. The test was compared with axenic culture examination, wet mount preparation and Papanicolaou stained smears. Four-hundred and fifty cases symptomatic and symptomatic were collected over two years. Out of 290 symptomatic women with cervicovaginitis and 160 asymptomatic women, 35 were culture positive for trichomoniasis. All culture positive specimens were PCR-positive giving a single product at 290 bp by agarose gel electrophoresis and recording 100% sensitivity, similar to culture examination. Among the 35 culture positive specimens, 12 were positive by wet mount and 21 were positive by Pap smear giving a 34.2% and 60% sensitivity, respectively. The standard and boiling DNA extraction methods were equally reliable but the latter was more simple, rapid and cheap. No specimens negative by PCR for trichomoniasis were positive by culture, wet mount or Pap smear. Moreover, specimens from cases with cervicovaginitis of non-trichomonal origin were negative by PCR. All samples of extracted DNA of T. vaginalis from positive culture tubes, used as positive controls, were also PCR positive at the expected product in gel, while no PCR product was detected with the negative DNA control including Chylamidia trachomatis and human DNA. It was found that the one-tube nested-PCR targeting the Tv-E650 repeat family of T. vaginalis is a simple, rapid sensitive and specific accurate method for diagnosis of symptomatic and asymptomatic vaginal trichomoniasis when applied to vaginal discharge.

Adolescent↗

PubMed1965