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Dark-cycle video surveillance of sexual performances of normal and diabetic rats.

Clinically, a 59% prevalence of impotence was reported among diabetic male patients. Neurological, vascular, endocrinologic and psychological factors are probably involved. Previous reports with the rat model found deterioration of sexual behavior and reproductive function caused by streptozotocin (STZ)-induced diabetes. The purpose of the present study was to develop the dark-cycle video recording methodology for the observation of rat sexual activities and to study the effect of STZ-induced diabetes on sexual performances of the rat. Adult male Wistar rats were rendered diabetic with intraperitoneal injection of STZ (60 mg/kg body weight). In the 4th week a diabetic rat or a control rat was caged with an adult ovariectomized female rat during the dark cycle. The female had been brought into behavioral estrus with intramuscular injection of 0.1 mg estradiol benzoate 3 days before and 1.0 mg progesterone 3 h before testing. Infrared-light-illuminated video recording was performed to evaluate the sexual performances. The mounting latency and frequency, intromission latency and frequency, the hit rate as well as the post-ejaculatory period of the diabetic rats were significantly deteriorated when compared with the controls (p < 0.05). However, the ejaculatory latency showed no significant difference between the two groups (p > 0.05). In conclusion, it has been demonstrated that with this methodology, behavioral studies on nocturnal animals like the rat can be carried out conveniently. It was shown that the sexual arousal mechanism and copulation-ejaculatory mechanism were both depressed in STZ-induced diabetic rats. The same study model can be used for further pathophysiological and pharmacological researches on the sexual behaviors of diabetic rats.

Animals↗

Readaptation of fish to 1g after long-term microgravity: behavioural results from the STS 89 mission.

The swimming behaviour of adult and neonate swordtail fish Xiphophorus helleri was qualitatively analysed from video recordings taken throughout the STS 89 spaceshuttle mission from launch to landing and thereafter. After the flight, the swimming behaviour of neonate samples was quantitatively assessed in the course of the readaptation to 1g earth gravity at days 0, 1 and 4 after recovery. Regarding the swimming behaviour during the mission, the adult fish swam thigmotactically (i.e., responding to tactile stimuli) along the walls of their aquarium, but like the neonates, they did not show any aberrant behavioural patterns. This indicates that they could easily adapt themselves to microgravity. On mission day 9, however, looping responses (most probably initiated by mechanical disturbances) occurred indicating a continuously performed "C-start" escape response (the respective body bend looks like the letter "C"). Immediately after landing (observed in videos recorded onboard the space shuttle), the adults performed a head-up swimming beating heavily with the caudal and pectoral fins; this aberrant behaviour gradually decreased during the first hours after recovery.

Adaptation, Physiological↗

Automated morphometry of corneal endothelial cell: use of video camera and video tape recorder.

We developed an apparatus for automated morphometry of the corneal endothelium, which was photographed through a specular microscope connected to a video camera, and the images were stored on a video tape. The clearest stationary image was input into an image analyser to determine automatically the cell boundaries. Although human interaction is generally necessary, the mean time required to complete this procedure was about 13 minutes, based on the results of the 30 normal eyes, and the time needed for manual correction was about 4 minutes. The mean cell area obtained by this method correlated well (r = 0.9335) with that obtained by tracing the same images. This apparatus is clinically useful for immediately obtaining the mean cell area of corneal endothelium and will extend the application of specular microscopy to the routine clinical setting.

Adult↗

Acquisition of a limited lifespan by differentiating cells derived from PC13 embryonal carcinoma cells.

Retinoic acid (RA) has previously been shown to induce the differentiation of mouse embryonal carcinoma (EC) cells to endoderm-like cells that have a slower rate of proliferation and are nontumorigenic. These cells also acquire the ability to respond to a range of exogenous growth factors. We have analysed the change in growth phenotype for PC13 EC cells using video recordings and autoradiography. We have shown that the endoderm-like cells have a longer cell cycle time than their undifferentiated counterparts (five cell divisions after exposure to RA the differentiated cells had a median cell cycle time of 1800 min compared to 800 min for control cells). The endoderm-like cells also have a progressively decreasing probability of dividing again and this indicates that the differentiation process is accompanied by the acquisition of a limited life-span. The characteristics of mortal cells are well documented, and the endoderm-like cells demonstrate the properties of such cells. In addition, we have confirmed the observation that epidermal growth factor (EGF) can stimulate the proliferation of the endoderm-like cells and have shown, using autoradiography, that 92% of these cells express EGF receptors. Using video recordings, we have demonstrated that the effect of EGF is to shorten the cell cycle of the differentiating cells. We have also shown that EGF can enhance the survival of the endoderm-like cells and thereby prolong their life-span. It is known that EGF and other growth factors can prolong the life-span of mortal cells derived from normal tissues, but we have demonstrated that EGF can have this effect on the differentiated derivatives of a tumour cell.

Animals↗

[Possible errors in diagnosis of left atrial thrombosis in transesophageal echocardiography].

The detection of thrombi in the left atrium and left atrial appendage by transesophageal echocardiography has significant diagnostic and therapeutic value. However, it requires high method accuracy in routine evaluation. In order to record the percentage of false positive findings, a reevaluation was performed by two experienced examiners using the video recordings of 51 patients, in whom a thrombus has been diagnosed during the routine evaluation. From 1988 to 1994, these thrombi were diagnosed in 726 patients in whom we looked for a source of possible cardiac embolism. In the reevaluation of the 43 cases with sufficient video recordings, in 26 (60%) the diagnosis was classified as false-positive and in 5 patients (12%) as questionable. Thrombus diagnosis was confirmed in only 12 (28%) cases. The incidence of false-positive findings decreased over the course of years with 8.3% (1988-1990), 2.9% (1991-1992) and 0.4% (1993-1994), p = 0.0001. The agreement in the reevaluation between the two examiners was high (interobserver variability 7%), whereby completely divergent evaluations did not occur. Typical diagnostic pitfalls included a prominent trabecular structure in the left atrial appendage (n = 10), the membranous structure between the left atrial appendage and the upper left pulmonary vein (n = 7), transition of the lower left atrium/left atrial appendage (n = 3) and branches within the apex of the left atrial appendage (n = 2). When searching for thrombi in the left atrium and left atrial appendage, there is a high risk of false-positive findings, especially for the unexperienced investigator. Attention must be paid to the typical causes of diagnostic pitfalls in the case of complex and variable anatomy.

Adolescent↗

Ictal technetium-99m ethyl cysteinate dimer single-photon emission tomographic findings and propagation of epileptic seizure activity in patients with extratemporal epilepsies.

Although ictal single-photon emission tomography (SPET) with technetium-99m ethyl cysteinate dimer (ECD) has a well-established role in the diagnostic evaluation of patients with temporal lobe epilepsy who are being considered for epilepsy surgery, its use in cases of extratemporal epilepsy is still limited. We investigated the influence of the propagation of extratemporal epileptic seizure activity on the regional increase in cerebral blood flow, which is usually associated with epileptic seizure activity. Forty-two consecutive patients with extratemporal epilepsies were prospectively evaluated. All patients underwent ictal SPET studies with simultaneous electroencephalography (EEG) and video recordings of habitual seizures and imaging studies including cranial magnetic resonance imaging and positron emission tomography with 2-[18F]-fluoro-2 deoxy-d-glucose. Propagation of epilptic seizure activity (PESA) was defined as the absence of hyperperfusion on ictal ECD SPET in the lobe of seizure onset, but its presence in another ipsilateral or contralateral lobe. Observers analysing the SPET images were not informed of the other results. PESA was observed in 8 of the 42 patients (19%) and was ipsilateral to the seizure onset in five (63%) of these eight patients. The time between clinical seizure onset and injection of the ECD tracer ranged from 14 to 61 s (mean 34 s). Seven patients (88%) with PESA had parieto-occipital epilepsy and one patient had a frontal epilepsy. PESA was statistically more frequent in patients with parieto-occipital lobe epilepsies (58%) than in the remaining extratemporal epilepsy syndromes (3%) (P<0.0002). These findings indicate that ictal SPET studies require simultaneous EEG-video recordings in patients with extratemporal epilepsies. PESA should be considered when interpreting ictal SPET studies in these patients. Patients with PESA are more likely to have parieto-occipital lobe epilepsy than seizure onset in other extratemporal regions.

Adolescent↗

High-speed video analysis of wing-snapping in two manakin clades (Pipridae: Aves).

Basic kinematic and detailed physical mechanisms of avian, non-vocal sound production are both unknown. Here, for the first time, field-generated high-speed video recordings and acoustic analyses are used to test numerous competing hypotheses of the kinematics underlying sonations, or non-vocal communicative sounds, produced by two genera of Pipridae, Manacus and Pipra (Aves). Eleven behaviorally and acoustically distinct sonations are characterized, five of which fall into a specific acoustic class of relatively loud, brief, broad-frequency sound pulses, or snaps. The hypothesis that one kinematic mechanism of snap production is used within and between birds in general, and manakins specifically, is rejected. Instead, it is verified that three of four competing hypotheses of the kinematic mechanisms used for producing snaps, namely: (1). above-the-back wing-against-wing claps, (2). wing-against-body claps and (3). wing-into-air flicks, are employed between these two clades, and a fourth mechanism, (4). wing-against-tail feather interactions, is discovered. The kinematic mechanisms used to produce snaps are invariable within each identified sonation, despite the fact that a diversity of kinematic mechanisms are used among sonations. The other six sonations described are produced by kinematic mechanisms distinct from those used to create snaps, but are difficult to distinguish from each other and from the kinematics of flight. These results provide the first detailed kinematic information on mechanisms of sonation in birds in general, and the Pipridae specifically. Further, these results provide the first evidence that acoustically similar avian sonations, such as brief, broad frequency snaps, can be produced by diverse kinematic means, both among and within species. The use of high-speed video recordings in the field in a comparative manner documents the diversity of kinematic mechanisms used to sonate, and uncovers a hidden, sexually selected radiation of behavioral and communicative diversity in the Pipridae.

Animal Communication↗

Upper airway dysfunction associated with collapse of the apex of the corniculate process of the left arytenoid cartilage during exercise in 15 horses.

OBJECTIVE: To report dynamic collapse of the apex of the left corniculate process under the right corniculate process into the airway at the dorsal apposition of the paired arytenoid cartilages during exercise as a cause of upper airway dysfunction in horses. DESIGN: Retrospective study. ANIMALS: Fifteen horses with a history of poor performance and/or upper respiratory tract noise during exercise. METHODS: Video recordings of all horses referred for upper airway evaluation using high-speed treadmill videoendoscopy (HSTV) between January 1998 and December 2003 were reviewed. Records of horses that developed dynamic collapse of the apex of the left corniculate process into the airway were included. Clinical history, age, gender, breed, and use of the horse were retrieved. RESULTS: Of 309 horses referred for examination for poor performance and/or upper respiratory tract noise during exercise, 15 (4.9%) had collapse of the apex of the left corniculate process under the right and into the airway at the dorsal apposition between the paired arytenoid cartilages during HSTV. There were 3 females and 13 males, aged from 2 to 5 years. Five horses had previous surgery for left recurrent laryngeal neuropathy (RLN): 2 had nerve muscle pedicle graft and 3 had laryngeal prosthesis. During HSTV, all 15 horses had progressive collapse of the apex of the left corniculate process under the right at the dorsal apposition of the 2 arytenoid cartilages, and into the dorsal aspect of the rima glottidis. Review of video recordings revealed that collapse of the apex of the corniculate process was followed by progressive collapse of the left aryepiglottic fold and left vocal fold. The ventral aspect of the left corniculate cartilage maintained abduction in all horses. Two horses also had progressive collapse of the right vocal fold, 1 had rostral displacement of the palatopharyngeal arch, and another had dorsal displacement of the soft palate. CONCLUSIONS: Dynamic collapse of the apex of the left corniculate process of the arytenoid cartilage under the right is an uncommon cause of upper airway dysfunction in horses and the pathogenesis is unclear. We speculate that the left arytenoideus transversus muscle is unable to support the dorsal apposition between the arytenoid cartilages. This loss of support allows the elastic cartilage of the left corniculate process to collapse under the right and into the airway, as inspiratory pressure increases during exercise. This condition may be associated with an unusually advanced neuropathy of the adductor components of the left recurrent laryngeal nerve and may be an unusual manifestation of RLN; however, this is speculative and further investigation is required to determine its cause. CLINICAL RELEVANCE: Dynamic collapse of the apex of the left corniculate process and into the airway at the dorsal apposition between the paired arytenoid cartilages can only be diagnosed during HSTV. It is an uncommon cause of upper airway dysfunction but may affect the athletic potential of racing Thoroughbreds and Standardbreds.

Animals↗

Arthroscopy -- a potential "gold standard" for the diagnosis of the chondropathy of early osteoarthritis.

OBJECTIVES: The aims of this study were to: 1. Evaluate the performance of arthroscopy for the diagnosis of chondropathy and to compare it to that of direct non-arthroscopic assessments; 2. Determine intra-observer reliability of arthroscopic assessments; 3. Evaluate the effects of the arthroscopic video quality and probing upon diagnostic performance. DESIGN: The ovine medial meniscectomy (MMx) model of early osteoarthritis (OA) was used assuming that pre-MMx articular cartilage (AC) was "normal" and post-MMx AC "chondropathic". Video recordings of arthroscopic assessments of each stifle compartment were evaluated. Scores were given for the quality of the video and the amount of probing. The diagnostic performances of dynamic shear modulus (G), light microscopic assessment and superficial zone collagen birefringence assessments were evaluated and compared to that of arthroscopy. Intra-observer reliability of arthroscopic assessments was also evaluated. RESULTS: Arthroscopic assessments had high sensitivity (91-100%), specificity (62-88%) and accuracy (75-93%) for the diagnosis of chondropathy 16 weeks after MMx. Arthroscopy compared favourably with the direct non-arthroscopic assessments in the lateral compartment and was found to have extremely high intra-observer reliability (kappa 0.78-1.00). The quality of arthroscopic video recordings and the amount of probing did not significantly influence accuracy or reliability. CONCLUSIONS: Arthroscopy performs as well as direct non-arthroscopic assessments of AC for diagnosis of early OA. These results suggest that arthroscopy can be used as a "gold standard" for the validation of non-invasive assessments like magnetic resonance imaging and that arthroscopic diagnosis can be based on small amounts of video footage without AC probing.

Animals↗

Efficacy of body mechanics education on posture while computing in middle school children.

OBJECTIVE: The purposes of this pilot study were to examine the working position of middle school children during their media/technology class, test student's knowledge of healthy computing habits, and evaluate the efficacy of two educational interventions compared to a control group. METHOD: A multi-group pre-test/post-test design was used. 19 students (mean age 11.6) participated in the study during their media/technology class. Students' postures while computing were evaluated on-site. Additionally five students were video recorded while they used the classroom computers so that on-site observations could be compared to video recorded data. All participants were given a written pre-test prior to educational interventions. The 12-minute educational intervention session focused on healthy computing habits, the importance of stretching, and group performance of specific upper extremity stretches. Additional education regarding overall postural health including good body mechanics was provided to one intervention group. RESULTS: The results of this study indicate that a twelve-minute educational intervention significantly improved participants' knowledge of healthy computing habits as measured by written pre-tests and post-tests. Participants who received additional education on overall postural health and body mechanics demonstrated the largest rate of improvement in positioning while computing. No statistical difference between intervention groups was noted on post-test scores, which attempted to measure long-term learning of healthy computing habits. CONCLUSION: Although more research in this area is warranted, it appears that education regarding correct positioning at computer workstations combined with instructing students on the importance of overall healthy posture may foster correct positioning while students use computers at school.

Adolescent↗

Computerized knowledge bases in primary health care: a curse or a blessing for health promotion, prevention and patient quality?.

This paper presents a future scenario analysis of how the introduction of computerized knowledge bases (KBs) can come to affect primary care practice. For the collection and analysis of data, a two-level video method was applied. First, four consultations where a computerized KB was used were video-recorded. A search workshop was then carried out by letting a multi-disciplinary panel comment on the video recordings. The comments were categorized with regard to content and perspective. Analyses of the comments showed a concern for a disregard of patients' health beliefs and for difficulties in portioning out the acquired medical knowledge to the patient during the consultation. Furthermore, the computerized KB was found to easily break the natural flow of the consultation and be perceived as a third party. The conclusion is that the most critical aspects for using computerized KBs in a reformed primary health care concern the integration of the systems into the consultation process. Health promotion, prevention, and patient quality are central here, and the introduction of KB technology must not lead the consultation away from these issues.

Artificial Intelligence↗

Quantification of neutrophil adhesion to skeletal muscle venules following ischemia-reperfusion.

Ischemia-reperfusion is encountered in a wide variety of surgical situations. The damage resulting from ischemia-reperfusion may be due, in part, to the infiltration and activation of neutrophils into the reperfused tissue. The purpose of the study was to examine ischemia-reperfusion induced neutrophil activation in skeletal muscle. In control muscles, ischemia in the hamster right cremaster muscle was produced for 5 min after an initial 2 hr 55 min perfusion period. In ischemic muscles, ischemia was produced for 3 hr prior to reperfusion. After the clamps were removed, a video recording of the cremaster microvasculature was made using intravital fluorescence microscopy. Acridine orange was infused intravenously 10 min prior to video recording in order to selectively label and enhance the contrast of neutrophils. The number of neutrophils rolling along the endothelium of 40 to 60 microns-diameter venules in a 1-min period increased from 9.0 in control animal cremaster venules to 24.1 following ischemia-reperfusion (p less than .05; n = 11). The ischemia-reperfusion model developed in this study allows for the direct quantification of neutrophil adhesion in skeletal muscle and can be further used to assess pharmacologic minimization of neutrophil-mediated damage in skeletal muscle.

Animals↗

[Effects of the training in integrity-promoting care on the interactions in a long-term ward].

A training programme in integrity-promoting care was given the staff of a long-term ward and practised during a three months intervention period. The effects were analysed in different parameters and compared with a control ward. This report describes analysis of video-recorded interactions (12 hs) during social activities between demented patients and caregivers and changes induced by the programme. The video-recorded episodes, containing mainly music and coffee sessions were interpreted from a model on interaction with demented patients suggested by Athlin and Norberg. The results indicate that the training programme and the guidance during the intervention resulted in increased understanding of demented patients' situation among the staff. They became more sensitive, adapted their cues and made the environment easier to interpret for the patients. This in turn led to the fact that the patients appeared more sensitive and clear in their cues. A positive circle developed.

Empathy↗

A study of patient facial expressivity in relation to orthodontic/surgical treatment.

A dynamic analysis of the faces of patients seeking an aesthetic restoration of facial aberrations with orthognathic treatment requires (besides the routine static study, such as records, study models, photographs, and cephalometric tracings) the study of their facial expressions. To determine a classification method for the units of expressive facial behavior, the mobility of the face is studied with the aid of the facial action coding system (FACS) created by Ekman and Friesen. With video recordings of faces and photographic images taken from the video recordings, the authors have modified a technique of facial analysis structured on the visual observation of the anatomic basis of movement. The technique, itself, is based on the defining of individual facial expressions and then codifying such expressions through the use of minimal, anatomic action units. These action units actually combine to form facial expressions. With the help of FACS, the facial expressions of 18 patients before and after orthognathic surgery, and six control subjects without dentofacial deformation have been studied. I was able to register 6278 facial expressions and then further define 18,844 action units, from the 6278 facial expressions. A classification of the facial expressions made by subject groups and repeated in quantified time frames has allowed establishment of "rules" or "norms" relating to expression, thus further enabling the making of comparisons of facial expressiveness between patients and control subjects. This study indicates that the facial expressions of the patients were more similar to the facial expressions of the controls after orthognathic surgery. It was possible to distinguish changes in facial expressivity in patients after dentofacial surgery, the type and degree of change depended on the facial structure before surgery. Changes noted tended toward a functioning that is identical to that of subjects who do not suffer from dysmorphosis and toward greater lip competence, particularly the function of the orbicular muscle of the lips, with reduced compensatory activity of the lower lip and the chin. The results of our study are supported by the clinical observations and suggest that the FACS technique should be able to provide a coding for the study of facial expression.

Adolescent↗

Collective responsibility for freeway rear-ending accidents? An application of probabilistic casual models.

Determining whether or not an event was a cause of a road accident often involves determining the truth of a counterfactual conditional, where what happened is compared to what would have happened had the supposed cause been absent. Using structural causal models, Pearl and his associates have recently developed a rigorous method for posing and answering causal questions, and this approach is especially well suited to the reconstruction and analysis of road accidents. Here, we applied these methods to three freeway rear-end collisions. Starting with video recordings of the accidents, trajectory information for a platoon of vehicles involved in and preceding the collision was extracted from the video record, and this information was used to estimate each driver's initial speed, following distance, reaction time, and braking rate. Using Brill's model of rear-end accidents, it was then possible to simulate what would have happened, other things being equal, had certain driver actions been other than they were. In each of the three accidents we found evidence that: (1) short following headways by the colliding drivers were probable causal factors for the collisions, (2) for each collision, at least one driver ahead of the colliding vehicles probably had a reaction time that was longer than his or her following headway, and (3) had that driver's reaction time been equal to his or her following headway, the rear-end collision probably would not have happened.

Accidents, Traffic↗

Understanding between care providers and patients with stroke and aphasia: a phenomenological hermeneutic inquiry.

Understanding between care providers and patients with stroke and aphasia: a phenomenological hermeneutic inquiry The present study illuminates the understanding in communication between formal care providers and patients with stroke and aphasia. Five care providers and three such patients participated in the study. Video recordings were made during conversations about pictures (n = 15), and the care providers were also interviewed (n = 15) after the video-recorded conversations. A phenomenological hermeneutic method of interpretation of the interview text was used. The findings showed that a range of conditions for 'understanding and being understood' in the communication on the part of the care providers exists. These different conditions are: lacking both knowledge and understanding; having knowledge but not necessarily accompanied by understanding; and being in understanding. Within the condition 'being in understanding', the care providers create a feeling of at-homeness in a relaxed atmosphere and thus have the opportunity to be in 'understanding and being understood' together with the patient. The condition 'being in understanding' appears in connection with the care providers' creating of a 'calm liturgy of caring' by mediating humility and calm vitality affects to the patients, and further, when needed, being present on the level of mystery, i.e. caring communion.

Adult↗

[Efficacy of a training technique concerning clinical interview skills associated with psychodiagnostic ability of the physician].

OBJECTIVE: To evaluate the efficacy of a training technique to improve clinical interview skills connected with Primary Care doctors' psycho-diagnostic ability: visual contact, posture, verbal and non-verbal ways of advancing the interview, verbal interruptions and questions with a psychological content. DESIGN: The doctors were randomly divided into two groups. A before-and-after design with blind evaluations was used. SETTING: University postgraduate Internal Medicine Course. MATERIAL: 20 doctors. INTERVENTIONS: Step-by-step training with feedback, using role-play and video-recording (experimental) was compared with a self-administered teaching programme (self-training). MEASUREMENTS AND RESULTS: Skills were measured by video-recordings of a patient interview one month before and one month after the intervention. The Observation Scale of Doctor's Skills was used. In the second evaluation, the experimental group differed from the self-training group in visual contact, posture and non-verbal ways of advancing the interview. CONCLUSIONS: The efficacy of a training technique in improving non-verbal active listening skills was shown. It was valuable as a support to the psycho-diagnostic ability of the doctor.

Adult↗