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PubMed1981

Histological and histochemical studies on the fundic mucosa of the camel's stomach (Camelus dromedarius).

The one-humped camel, like our domestic ruminants, has a capacious compartmentalized stomach and is a ruminating animal. In our studies wer followed the currently predominant opinion that the camel stomach consists of three compartments (Vallenas, cummings, Munnel 1971). The third one of it is considered as the tubular portion following the second compartment or reticulum and extends till to the pylorus. The Tunica mucosa in the terminal dilated part (distal one-fifth) of this third compartment bears at its greater curvature very thick folds enclosing flat furrows which divide the mucosa into nearly regular areas (gastric areas). This portion of the third compartment contains the proper gastric glands (fundic glands) which differ somewhat from those in the advanced ruminants, especially in the arrangement of the parietal cells and the behaviour of the chief cells which can store mucosubstance during fastening or diminishing of the food in the camel.

Animals↗

PubMed1982

The submucosal glands and the orientation of the musculature in the oesophagus of the camel.

The histological structure of the oesophagus of the camel in different parts of its course was investigated with tissues from seven camels, and with haematoxylin and eosin and Masson's trichrome stain. Gross dissections confirmed the orientation of the muscle fibres in the tunica muscularis. The epithelium showed heavy cornification. Many submucosal mucous glands were found throughout the length and all round the wall of the oesophagus. This was contrary to what has been reported generally for ruminants. The lamina muscularis mucosae was in the form of small scattered strands of smooth muscle (more readily identifiable with trichrome stain) and only in the caudal oesophagus. It did not form a continuous layer here. The tunica muscularis was of striated type throughout which agreed with what has been found in the other ruminants. Its fibres, however, were differently oriented. The outer tunica was mainly circular (especially in the mid-thoracic part) and the inner one was mainly longitudinal in direction. Mixed orientation of the muscle fibres in parts of the same section was also noticed. These findings have not been reported previously in the camel.

Animals↗

PubMedDate not supplied

[Effectiveness of peptic ulcer diet therapy using rations containing whole mare's and camel's milk].

Diets enriched with whole mare and camel's milk were used for the management of peptic ulcer patients. A total of 164 patients were examined. Of these, 59 received mare's milk, 40 camel and 65 cow's milk. On the basis of studying the time course of the clinical picture, secretory and motor functions of the stomach, as well as of the endoscopic appearance of the gastric and duodenal mucosa it was ascertained that apart from the improved clinical course of the disease, secretory and motor functions of the stomach there was a complete wound healing and remarkable decline of its size in 93, 90, and 70% of patients given mare, camel and cow's milk, respectively. Also, radiotelemetry was used to study the antacid properties of the milk types in question. The first two milk types have demonstrated more pronounced antacid properties.

Adult↗

PubMed1995

Seasonal prevalence of Onchocerca-like microfilaria in camels in Kuwait.

Infection of the camel (Camelus dromedarius) with Onchocerca-like microfilaria is recorded for the first time in Kuwait. Throughout a period of 14 months, the blood of 284 camels were examined, of these 32 (11%) were found infected with the microfilaria. Infections were recorded in all months except during summer, July to September, and in February. The highest monthly incidence (27%) was detected in June at the end of spring. It is suggested that harsh environmental conditions in Kuwait during summer either arrest development of microfilariae or influence their diurnal distribution in tissues of the camel.

Animals↗

PubMed1994

First isolation of Trichophyton verrucosum as the aetiology of ringworm in the Sudanese camels (Camelus dromedarius).

A survey was conducted to study camel ringworm in Eastern Sudan. Ringworm was diagnosed in 217 out of 498 young camel calves under two years old examined during a whole year (43.5%). The peak incidence of the disease was found to be in Autumn and Winter. The disease was observed more frequently among young growing calves (1-2 years) than older animals but the prevalence among male and female animals was found to be similar. Lesions were observed mainly on the head, neck and shoulder with frequent extension to the flanks and limbs. Trichophyton verrucosum was isolated in pure culture for the first time from camel ringworm in the Sudan. Histopathological findings of the natural disease are described. Epidemiology in Eastern Sudan is discussed.

Animals↗

PubMed1994

[Efficacy and residual effect of amitraz (Taktic) on adult Hyalomma dromedarii in camels: preliminary trial].

Amitraz (12.5% emulsifiable concentrate) was tested against Hyalomma dromedarii of the one-humped camel in a dairy camel herd in Nouakchott (Mauritania), at a concentration of 0.025% of the active component. The efficacy on adult ticks and the rapid rate of tick detachment are clearly shown: 95% reduction within eight hours; however, the nymphs seem to be more resistant: only 50% reduction after eight hours. The residual activity of amitraz against Hyalomma dromedarii in camels is very short, less than 24 hours; furthermore, the amitraz treatment has no effect on survival, oviposition and the rate of egg hatching of females which attach in the first days following treatment. A discussion of likely causes of this phenomenon is presented.

Animals↗

PubMed1994

[Evaluation of a direct serologic card agglutination test for the diagnosis of camel trypanosomiasis caused by Trypanosoma evansi].

The results of a novel direct serological card agglutination test for the diagnosis of camel trypanosomosis due to Trypanosoma evansi (CATT/T. evansi) were compared with those obtained by direct detection of parasites in a study using 1,093 sera from camels raised in northern Mali. A good correlation was revealed between the percentage of positive results obtained by CATT and the presence of trypanosomes (89%), as well as a good coincidence between the percentage of positive results obtained by CATT and low haematocrit values (packed cell volume). CATT revealed a global serological prevalence of 30.6%, whereas trypanosomes were found in only 5.85% of the corresponding animals. CATT/T. evansi is a quick and easy-to-read test, which merits further evaluation in camel-rearing countries.

Age Factors↗

PubMed1994

[Camel trypanosomiasis caused by Trypanosoma evansi (Steel 1885), Balbiani 1888, in Islamic Republic of Mauritania: results of surveys in the Trarza region].

A first survey concerning camel trypanosomiasis due to Trypanosoma evansi was carried out in the Trarza region (south-western Mauritania). Blood smears were made from 218 animals and 160 were submitted to an indirect immunofluorescence test. Young calves below one year old seem to be free of T. evansi infection, while in dairy females, average rates of infection of 7.3% (blood smears) and 24.5% (serological results) were found. The type of herd management seems to be an important risk factor: the herds that frequent, even temporarily, the wooded areas of the Senegal river valley or permanent water collections (R'kiz lake) show the highest infection rates. Recommendations are presented to camel rearing owners, especially those with dairy camel herds in the South of the country.

Animals↗

PubMed1993

Presence of ketones in the serum of Trypanosoma evansi infected camels (Camelus dromedarius) in the Sudan.

One hundred serum and two urine samples collected from camels (Camelus dromedarius) were analysed at the Eastern State Veterinary Research Laboratory, Kassala, Sudan, for the presence of ketones. All fifty sera from trypanosome infected camels gave positive results. Fourty five out of the fifty serum samples of trypanosome negative camels showed negative results. The five positive samples were also positive with the mercuric chloride test.

Animals↗

PubMed1993

An ultrastructural study of the erythrocytes associated with haematochemical data of the camel Camelus dromedarius.

The aims of the present study were to characterize by electron microscopy the morphological features of the erythrocytes of normal adult and young camels Camelus dromedarius in a renal blood vessel and in a smear. These cells are thin and elliptical, with a slight central elevation. They are slightly larger, but thinner than those reported by other authors. It is found that the renal blood vessels in adults have a thicker basal lamina with the presence of electron dense membranous bodies. These two features were described in different parts of the uriniferous tubules of the camel in previous studies. The numerical data of the haematochemical parameters that are reported here are in general similar for both adult and young camels, while they differ from those of other studies, this may have resulted from their unknown provenance and their previous dietary regime. Moreover, these parameters probably relate to the arid environment of the animal.

Adaptation, Physiological↗

PubMed1995

Relations between udder infection and somatic cells in camel (camelus dromedarius) milk.

Quarter milk samples (n = 391) from 101 camels were examined to study the occurrence and causes of mastitis in traditionally managed camels in eastern Sudan and to evaluate the value of the California Mastitis Test (CMT), somatic cell count (SCC) and adenosine triphosphate (ATP) in the detection of subclinical mastitis in the camel. One hundred and seventy (43.5%) of the quarter milk samples yielded pathogenic bacteria. Streptococcus agalactiae, other Streptococcus spp., Staphylococcus aureus, coagulase-negative staphylococci, and Escherichia coli were isolated from milk. Thirty-two (8.2%) quarter milk samples yielded mixed cultures, and 189 (48.3%) yielded no growth. Mean values for CMT, SCC and ATP were higher for quarters infected with major pathogens. However, a significant number of quarter milk samples had elevated values in these tests but were from quarters from which no bacteria were isolated. The ability of the tests to predict a positive bacteriology increased slightly when 2 or 3 tests were combined.

Animals↗

PubMed1998

Diagnosis of trypanosomiasis in experimental mice and field-infected camels by detection of antibody to trypanosome tyrosine aminotransferase.

Sera from animals with acute and chronic Trypansoma evansi infections were examined directly for trypanosome tyrosine aminotransferase activity and indirectly for their ability to inhibit tyrosine aminotransferase activity. It was shown that sera from acutely infected mice and camels with high parasitemias contained significant levels of trypanosome tyrosine aminotransferase activity. In contrast, the sera from chronically infected mice and camels did not contain significant tyrosine aminotransferase activity, but they were able to neutralize the enzyme activity in trypanosome homogenates. The sera from camels with other pathological conditions did not neutralize this enzyme activity. It is suggested that the inhibitory factor in the chronic sera is antibody. The potential use of the direct enzyme assay and the indirect neutralization assay as diagnostic tools are discussed. Finally, the use of these assays to distinguish between early (acute) and late (chronic) infections are also suggested.

Animals↗

PubMed1998

Camel single-domain antibody inhibits enzyme by mimicking carbohydrate substrate.

Whereas antibodies have demonstrated the ability to mimic various compounds, classic heavy/light-chain antibodies may be limited in their applications. First, they tend not to bind enzyme active site clefts. Second, their size and complexity present problems in identifying key elements for binding and in using these elements to produce clinically valuable compounds. We have previously shown how cAb-Lys3, a single variable domain fragment derived from a lysozyme-specific camel antibody naturally lacking light chains, overcomes the first limitation to become the first antibody structure observed penetrating an enzyme active site. We now demonstrate how cAb-Lys3 mimics the oligosaccharide substrate functionally (inhibition constant for lysozyme, 50 nM) and structurally (lysozyme buried surface areas, hydrogen bond partners, and hydrophobic contacts are similar to those seen in sugar-complexed structures). Most striking is the mimicry by the antibody complementary determining region 3 (CDR3) loop, especially Ala104, which mimics the subsite C sugar 2-acetamido group; this group has previously been identified as a key feature in binding lysozyme. Comparative simplicity, high affinity and specificity, potential to reach and interact with active sites, and ability to mimic substrate suggest that camel heavy-chain antibodies present advantages over classic antibodies in the design, production, and application of clinically valuable compounds.

Animals↗

PubMed1997

Immunohistochemical identification of pancreatic hormones, neuropeptides and cytoskeletal proteins in pancreas of the camel (Camelus dromedarius).

The patterns of distribution of insulin (INS), glucagon (GLU), atrial natriuretic peptide (ANP), neuropeptide-Y (NPY), cholecystokinin-octapeptide (CCK-8), neurofilament-200 protein (NF), S-100 protein (S-100), and vimentin (VIM) in the pancreas of the one-humped camel (Camelus dromedarius) were investigated using immunohistochemical techniques. INS-immunoreactive cells were observed in the central and peripheral parts of the islets of Langerhans, but some solitary INS-positive cells were found outside the islets. INS-positive cells constituted 44.26-90.91% [mean +/- standard deviation (std): 67.34 +/- 14.20] of the total number of islet cells. GLU-immunopositive cells were located mainly in the peripheral region of the islets, and they constituted 11.43-44.44% [mean +/- std: 23.54 +/- 8.27] of the total number of islet cells. ANP and CCK-8 immunoreactivity was observed in neurons and perivascular nerves fibers. NPY was identified in pancreatic neurons and in some peripheral and central cells of the islets of Langerhans. VIM immunoreactivity was observed in the endothelial cells of blood vessels and the nerves located in the perivascular, interlobular and periacinar regions. VIM was also detected immunohistochemically in the periductal nerves of the pancreas. NF occurred only in nerves. S-100 was discerned mainly in the nerves of the interlobular connective tissue and in nerves lying close to blood vessels and acinar tissue. It is concluded that INS, GLU, ANP, NPY, CCK-8, NF, S-100, and VIM are well distributed in the pancreas of the camel.

Animals↗

PubMedDate not supplied

Unique single-domain antigen binding fragments derived from naturally occurring camel heavy-chain antibodies.

The humoral immune response of camels, dromedaries and llamas includes functional antibodies formed by two heavy chains and no light chains. The amino acid sequence of the variable domain of the naturally occurring heavy-chain antibodies reveals the necessary adaptations to compensate for the absence of the light chain. In contrast to the conventional antibodies, a large proportion of the heavy-chain antibodies acts as competitive enzyme inhibitors. Studies on the dromedary immunoglobulin genes start to shed light on the ontogeny of these heavy-chain antibodies. The presence of the heavy-chain antibodies and the possibility of immunizing a dromedary allows for the production of antigen binders consisting of a single domain only. These minimal antigen-binding fragments are well expressed in bacteria, bind the antigen with affinity in the nM range and are very stable. We expect that such camelid single domain antibodies will find their way into a number of biotechnological or medical applications. The structure of the camelid single domain is homologous to the human VH, however, the antigen-binding loop structures deviate fundamentally from the canonical structures described for human or mouse VHs. This has two additional advantages: (1) the camel or llama derived single domain antibodies might be an ideal scaffold for anti-idiotypic vaccinations; and (2) the development of smaller peptides or peptide mimetic drugs derived from of the antigen binding loops might be facilitated due to their less complex antigen binding site.

Animals↗

PubMed2000

Anatomical subdivisions of the stomach of the Bactrian camel (Camelus bactrianus).

Twenty stomachs of Bactrian camels (Camelus bactrianus) were studied by gross dissection. Based on the configuration of the stomach and the structure of the mucous membrane, the stomach was divided into three ventricles that differ from the arrangement described for ox and sheep. The first and second ventricles of the proventriculus of camel form one stomach rather than two different stomachs. These ventricles of the proventriculus do not correspond to the rumen and reticulum of ox and sheep. The third ventricle appears to be the abomasum. One part of the abomasum has reticular mucosal folds that indicate it is not the reticulum. A second part of the abomasum has longitudinal mucosal folds suggesting it is not the omasum. Three glandular sac areas associated with the preventriculus and abomasum are also described.

Abomasum↗

PubMed2000

Investigation of total, free, peptide-bound, protein-bound, soluble and insoluble collagen hydroxyproline content in tissues from the Arabian camel (Camelus dromedarius).

This study was conducted to determine the concentration of total, free, peptide-bound, protein-bound, soluble and insoluble collagen hydroxyproline (Hyp) in tissues from the Arabian camel (Camelus dromedarius). Results indicated that there were significant differences in the concentration of total, free, peptide-bound, protein-bound, soluble and insoluble collagen Hyp in various tissues (P < 0.01). Camel kidney showed a significantly high concentration of total, free, peptide-bound and protein-bound Hyp and collagen content as compared to other tissues examined (P < 0.01). Kidney also showed a significantly high concentration of soluble collagen Hyp as compared to other tissues examined (P < 0.01). However, the concentration of insoluble collagen Hyp was significantly high in liver when compared to other tissues (P < 0.01). These variations may result from differences in the collagen structure and/or composition in this species.

Animals↗

PubMed2006

A synthetic camel anti-lysozyme peptide antibody (peptibody) with flexible loop structure identified by high-resolution affinity mass spectrometry.

We describe the synthesis and characterisation of the fully functional molecular recognition structure of a 26-amino acid residue peptide antibody, referred to as peptibody, designed from a monoclonal single-domain antibody fragment derived from a camel heavy-chain antibody. The CDR3 region (CDR = complementarity determining region) of the cAbLys3 camel antibody fragment, which binds to the active site of hen eggwhite lysozyme (HEL) and acts as a potent enzyme inhibitor by mimicking an oligosaccharide substrate, was prepared by solid-phase peptide synthesis. To obtain a closed loop-like structure resembling that in the crystal structure, N- and C-terminal cysteine residues were added to the linear peptide and oxidised to a cyclic disulfide-bridged peptide by using dimethylsulfoxide. A further, internal cysteine-12 residue was acetamidomethyl-protected to prevent possible oxidative byproducts. Affinity separation on a lysozyme microcolumn combined with MALDI-TOF mass spectrometry revealed that the peptide resumed high affinity to lysozyme only after deprotection of Cys-12, suggesting the importance of this paratope sequence for epitope recognition. The complex of lysozyme and active peptibody was characterised directly by conducting high-resolution ESI-FTICR mass spectrometry, which provided a molecular comparison of affinities for linear and cyclic peptibodies.

Amino Acid Sequence↗