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At least 397 records · Page 22Linked to original sources
PubMed1999

Confocal microscopy of dentinal tubules in human tooth stained with alizarin red.

The present study was designed to analyze the structures of dentinal tubules by confocal microscopy. Undecalcified ground sections of human teeth were stained with alizarin red in 0.1% KOH aqueous solution, and examined by confocal microscopy. Alizarin red stained dentinal tubules, interglobular dentine, granular layer of Tomes, and the surface of dentine. Interglobular dentine was seen between the outer and middle layers of coronal dentine. At the outer layer of coronal dentine, the dentinal tubules were thin and showed numerous branches. At the middle layer of coronal dentine, dentinal tubules displayed two types. The type I tubules are the dentinal tubules that do not show any nodular structures and the type II tubules are the dentinal tubules that appear bamboo-like with many nodules. In the cross section through the type II tubules, the nodules appeared as fine circular tubules surrounding the dentinal tubules. The circular tubules of nodules adhered to one side of the dentinal tubules. When the fluorescence images were compared with the images taken by transmission light mode, the fluorescence of dentinal tubules was seen at the inner surface of dentinal tubules, and the fluorescence of nodules was seen at interface between peritubular and intertubular dentine. Most of the dentinal tubules were of the type II tubules in the teeth from older individuals, whereas the type II tubules were scarce in the teeth from younger individuals. At the inner layer of coronal dentine, the dentinal tubules have no nodules and branches were scarce. The dentinal tubules of radicular dentine were different from those of coronal dentine. Most of the dentinal tubules were the type I tubules. Numerous fine branches were seen at the outer and middle layers of radicular dentine. No interglobular dentine was seen in the root except at the cervical part, and the granular layer of Tomes was also positive with alizarin red. At the cervical part of the root, interglobular dentine was present and the dentinal tubules displayed types I and II.

Adult↗
PubMed2001

Demineralization of caries-affected transparent dentin by citric acid: an atomic force microscopy study.

OBJECTIVES: This study determined recession rates of peritubular dentin and intertubular dentin in citric acid solution (0.018M, pH=2.5) for caries-affected transparent dentin, one of the major components of dentin substrate as altered by caries, with comparisons made with non-carious dentin. METHODS: Transparent dentin was identified by sagittally sectioning five obviously carious teeth. Sections were then cut through the transparent dentin area perpendicular to the course of the dentinal tubules. Polished dentin samples of the transparent dentin and non-carious dentin were prepared with an internal reference layer and studied at specific intervals for citric acid etching in an atomic force microscope (AFM). RESULTS: At baseline, transparent dentin was identified by dentinal tubules that were largely occluded with mineral deposits that on etching proved to be acid resistant. Peritubular dentin etched rapidly and linearly over time until it could no longer be measured, yielding etching rates for transparent dentin that could not be distinguished from normal dentin. The normal and transparent intertubular dentin surfaces began etching at nearly the same rate, but then surface recession stabilized after less than 1 microm depth change for both dentin types. SIGNIFICANCE: Most previous studies have focused on demineralization and bonding to normal dentin, although many bonding procedures involve altered dentin substrates, such as those modified by caries. In this study, peritubular and intertubular dentin from normal and caries-affected dentin exhibited similar behavior. The major difference was the presence of acid resistant mineral in most of tubule lumens in the transparent dentin.

Acid Etching, Dental↗
PubMed2001

Qualitative and quantitative assessment of intratubular dentin formation in human natural carious lesions.

It was the aim of this study to investigate the scanning electron microscopy (SEM) features and the element content of calcium (Ca) phosphorus (P), magnesium (Mg), oxygen (O), and carbon (C) of sound dentin, demineralized dentin, secondary dentin, intratubular and peritubular de novo dentin formation due to caries progression and to compare the element content with chemically pure hydroxyapatite. Eighteen extracted teeth with deep dentin carious lesions were embedded in Technovit 9100 (Kulzer), and serial sections of 80 microm thickness were made. These sections were then investigated with polarized light microscopy to identify the lesions. Two sections of each lesion were then coated with carbon and studied with a scanning electron microscope. Of the 18 teeth, 8 showed intratubular dentin formation. The element content was measured using energy dispersive X-ray analysis (EDX). About 75% of all involved dentin tubules showed intratubular de novo dentin formation. The Ca/P ratio in sound dentin, demineralized dentin, peritubular dentin, and secondary dentin was within the range of hydroxyapatite, whereas in intratubular dentin the Ca/P ratio was different than that of hydroxyapatite. The element content for Ca was statistically highly significantly different (P < 0.01) among sound dentin/intratubular dentin, sound dentin/peritubular dentin, and sound dentin/ secondary dentin, but not between sound dentin/ demineralized dentin and sound dentin/hydroxyapatite. For the other measured elements varying statistical differences were found. Our results indicate that intratubular dentin does not occlude the dentin tubules completely and mineralizes different than that of sound dentin and may therefore not be true hydroxyapatite.

Dental Caries↗
PubMedDate not supplied

The effect on shear bond strength of rewetting dry dentin with two desensitizers.

The difficulty related to achieving a balance between wet and dry dentin makes the dentin bonding technique extremely sensitive. This study evaluated the effect of rewetting dried dentin with two commercial desensitizing agents (Protect and HurriSeal) on the dentin shear bond strength of three total-etch dentin bonding agents (Syntac Single-Component, OptiBond Solo Plus and Prime & Bond NT) and compared both to applying these same bonding agents to moist dentin and dry dentin. Each bonding agent was paired with an appropriate resin composite from the same manufacturer (Table 1). Recently extracted, formalin-treated caries-free human molars were used. The occlusal surface of each tooth was ground to create a flat dentin surface. Then, each tooth was mounted in acrylic. Twelve groups (n = 15) were prepared: 1) Syntac Single-Component with Heliomolar resin composite (SSC/H) to moist dentin; 2) SSC/H to dry dentin; 3) SSC/H to dried dentin rewet with Protect; 4) SSC/H to dried dentin rewet with HurriSeal; 5) OptiBond Solo Plus with Point 4 resin composite (OBS+/P4) to moist dentin; 6) OBS+/P4 to dry dentin; 7) OBS+/P4 to dried dentin rewet with Protect; 8) OBS+/P4 to dried dentin rewet with HurriSeal; 9) Prime & Bond NT with TPH Spectrum resin composite (PBNT/TPH) to moist dentin; 10) PBNT/TPH to dry dentin; 11) PBNT/THP to dried dentin rewet with Protect and 12) PBNT/TPH to dried dentin rewet with HurriSeal. Groups 1, 5 and 9 were placed according to manufacturers' instructions (moist dentin) as control groups. All the other groups received a 15-second air blast after etching and prior to applying the one bottle adhesive or desensitizer and one bottle adhesive. Resin composite cylinders [4 mm in diameter and 2 mm in height] were then placed. The specimens were stored in distilled water at 37 degrees C for 24 hours prior to thermocycling 2,500 times (at 8 degrees C and 48 degrees C). Shear bond strengths (SBSs) were measured one week after fabrication by using a circular knife-edge and crosshead speeds of 0.5 mm/minute. Failure modes of debonded specimens were determined under a stereomicroscope (30x). Failed specimens with the low and high shear bond strengths in each group were evaluated under a low vacuum Scanning Electron Microscope (SEM-LV). One-way ANOVA and Tukey's tests were used to compare the different conditions for each bonding system. In the Syntac Single-Component bonding agent groups, there was no significant difference in shear bond strength between the control (15.73 MPa), dry (18.11 MPa) and HurriSeal (16.18 MPa) specimens. Protect specimens showed significantly lower shear bond strength (6.39 MPa). In the OptiBond Solo Plus bonding agent groups, there was no significant difference between the control (20.79 MPa) and the HurriSeal (21.29 MPa) groups, and both groups had significantly greater bonds than the dry (14.13MPa) and Protect (9.57 MPa) groups. In the Prime & Bond NT bonding agent groups, the shear bond strength of the HurriSeal group (20.73 MPa) was significantly higher than the other groups: control (8.05 MPa), dry (5.73 MPa) and Protect (5.45 MPa).

Acrylic Resins↗
PubMedDate not supplied

Dentin bonding: effect of degree of mineralization and acid etching time.

This in vitro study verifies whether there are differences between bonding to hypermineralized dentin and normal dentin and if longer acid etching can improve the bond strength to this modified substrate without damaging the bond to normal dentin. Forty-two extracted human molars with chronic occlusal caries were transversally cut with a diamond saw under refrigeration. The occlusal surfaces were ground until the carious lesion was removed, exposing the sclerotic dentin in the center and polished to 600/grid. A 35% phosphoric acid (3M) was applied for 15 seconds in 15 specimens. SingleBond (3M) adhesive system was applied and a hybrid resin composite (Filtek Z250, 3M) was inserted in four 1-mm increments and light-cured. The remaining 15 molars were prepared in the same manner, but with an acid etching time of 30 seconds. After 24 hours in water, the specimens were cut in two perpendicular directions to obtain a cross section of approximately 0.7 mm2 (n=25). A visual examination was conducted to select sticks between the two groups: sclerotic dentin (G15S or G30S) and normal dentin (G15N or G30N). Sticks without 100% sclerotic dentin (translucent area) or those with normal areas were not tested. Two-way ANOVA computed the mu-TBS data taking into consideration dentin type and acid etching time. The dentin Knoop hardness number (KHN) of the sticks was verified. A t-test compared the KHN data between sclerotic and normal dentin. Twelve additional molars (n=6) were prepared to observe the interface under a SEM. The mean (+/- SD) microtensile bond strengths (mu-TBS) were: G15S=56.4(+/- 14.9), G15N=69.7(+/- 17.2), G30S=63.2(+/- 15.6) and G30N=67.7(+/- 13.3). Two-way ANOVA showed higher mu-TBS to normal dentin than sclerotic dentin. Duncan's Post Hoc showed G15N had higher mean mu-TBS than G15S. Other comparisons were not significantly different. The t-test showed statistically higher microhardness in sclerotic dentin than in normal dentin (p<0.0001). The hybrid layer (HL) formation was observed in all specimens without gap formation in any region. In sclerotic dentin (G15S), the HL was very thin, with minimal resin tags in the dentinal tubules and, when present, they were shorter. Doubling the etching time (G30S) resulted in more resin tags with an HL formation on peritubular dentin. The HL on normal dentin was thicker when it was acid etched for 30 seconds (G30N). Numerous resin tags were present with both etching times. The results suggest that the higher mineral amount in sclerotic dentin makes it difficult to bond to this substrate, resulting in a lower mu-TBS. However, doubling the etching time resulted in mu-TBS similar to normal dentin.

Acid Etching, Dental↗
PubMed1997

A light microscopic and ultrastructural examination of calcified dental tissues of horses: 3. Dentine.

Ultrastructural examinations of equine dentine found that dentinal tubules extended from the amelodentinal junction towards the pulp forming primary curvatures. The number of dentinal tubules/unit area and their diameters increased significantly from the amelodentinal junction towards the pulp cavities, particularly in regular secondary dentine, but irregular secondary (tertiary) dentine contained no dentinal tubules. Dentinal tubules contained odontoblast processes that appeared to extend as far as the amelodentinal junction, but due to iatrogenic loss during specimen preparation, odontoblasts were seldom found in regular secondary dentine. In primary dentine, the dentinal tubules were surrounded by large amounts of peritubular dentine that increased in diameter from the amelodentinal junction towards the junction of primary and secondary dentine. The site of the dentinal tubule within the peritubular dentine varied at different dentinal sites. Peritubular dentine was present in primary dentine only and was surrounded by a thin layer of intertubular dentine. When acid etched, peritubular dentine gave dentine a honeycomb appearance adjacent to the junction of primary and secondary dentine.

Animals↗
PubMed2000

Citric acid etching of cervical sclerotic dentin lesions: an AFM study.

Atomic force microscopy (AFM) has been used to determine microstructural changes, etching rates of peritubular dentin, and intertubular dentin recession during demineralization in dilute acidic solutions. These studies have not included many forms of altered dentin, including noncarious sclerotic root dentin associated with Cl V (abfraction) lesions. We sought to determine microstructural changes and recession rates during demineralization in citric acid (pH 2.5, 0.018M) for the transparent/sclerotic zone. Highly polished dentin disks were prepared from teeth with noncarious C1 V lesions (n = 3) and compared with normal root dentin (n = 3). Samples were etched at 5-s intervals for 1 min and at longer intervals up to 30 min. The depth changes in various portions of the dentin with respect to the reference layer were measured and changes in microstructure observed in solution in the wet cell of the AFM. In sclerotic dentin, most tubule lumens were occluded with crystalline deposits that etched more slowly than the other dentin components, but etching rates could not be determined due to their irregular geometry. The intertubular dentin recession quickly reached a plateau after a depth change of <1 microm for all dentin types, in agreement with prior work. Mixed linear regression models indicated an important difference between the etching of sclerotic intertubular dentin and that of non-sclerotic root dentin that became apparent after 600 s (p = 0.037). The sclerotic intertubular dentin underwent less depth change at the plateau (558 nm) compared to normal root dentin (744 nm). In addition, normal root dentin underwent significantly greater recession than coronal dentin (p = 0.002). The results of this study indicate that intertubular sclerotic dentin from Cl V lesions etches differently than normal root dentin, and this may help explain the difficulties in restoring such lesions with current bonding procedures.

Acid Etching, Dental↗
PubMedDate not supplied

A comparison of three resin bonding agents to primary tooth dentin.

This study determined the shear bond strength of resin composites to primary dentin using three dentin adhesives and the presence or absence of a hybrid zone. The buccal and lingual surfaces of 40 recently extracted noncarious primary teeth were ground flat with SiC paper ending with the 600 grit. The teeth were divided at randomly into eight groups of five teeth (10 surfaces) each: 1) Unetched dentin, dry dentin, All-Bond 2/Bis-Fil P; 2) Unetched dentin, moist dentin, All-Bond 2/Bis-Fil P; 3) Dentin etched for 15 sec with 10% phosphoric acid, dry dentin, All-Bond 2/Bis-Fil P; 4) Dentin etched for 15 sec with 10% phosphoric acid, moist dentin, All-Bond 2/Bis-Fil P; 5) Dentin etched with 10% maleic acid for 15 sec, dry dentin, Scotchbond Multi-Purpose/Z100; 6) Dentin etched with 10% maleic acid for 15 sec, moist dentin, Scotchbond Multi-Purpose/Z100; 7) Dentin etched with 10 citric acid/3% ferric chloride, dry dentin, Amalgambond Plus/Z100; 8) Dentin etched with 10 citric acid/3% ferric chloride, moist dentin, Amalgambond Plus/Z100. All teeth were thermocycled 1000x (5 and 55 degrees C, 30-sec dwell time), and shear bond strength testing was conducted using an Instron (crosshead speed 0.5 mm/min). Failure sites after debonding were examined with the SEM. For each group, one additional tooth was used to prepare two class V cavities (one facial and one lingual) restored according to the specification in each group, sectioned buccolingually and examined with the SEM. The results, in MPa, were: 1) 12.55 +/- 5.97; 2) 10.41 +/- 6.16; 3) 9.94 +/- 7.26; 4) 12.25 +/- 4.70; 5) 13.02 +/- 8.01; 6) 16.51 +/- 8.62; 7) 12.51 +/- 8.95; 8) 17.93 +/- 6.44. ANOVA and Student-Newman-Keuls tests showed no statistically significant differences. SEM evaluation showed that the smear layer was removed in all groups exposing primary dentin tubules infiltrated by resin. A resin-reinforced hybrid layer was readily seen in all specimens.

Acid Etching, Dental↗
PubMed1992

A scanning electron microscopic study on the distribution of peritubular dentine in cheek teeth of Cervidae and Suidae (Mammalia, Artiodactyla).

Distribution of peritubular dentine was studied in cheek teeth of fallow deer (Dama dama), roe deer (Capreolus capreolus) and wild boar (Sus scrofa). In the two cervid species, especially intense peritubular dentine formation was found in the outer regions of mid and cuspal coronal dentine. In these areas a marked asymmetry occurred, peritubular dentine being predominantly secreted onto the side of the dentinal tubule walls nearest to the dentinoenamel junction. Intensity and asymmetry of peritubular dentine formation decreased cervically. In root dentine, the walls of the dentinal tubules were covered with only a thin peritubular dentine layer of even thickness. Here, in contrast to peripheral coronal dentine, the volume of intertubular dentine far exceeded that of peritubular dentine. In porcine coronal dentine, PTD asymmetry, being of lesser extent than in cervids, was observed only in peripheral areas of cuspal and flank regions of the cheek teeth. Because peritubular is more highly mineralized than intertubular dentine, the relative volume of dentine made up from the two components has an important influence on dentinal wear resistance. The significance of variations in volume and distribution of peritubular dentine between different dentinal regions for achieving and maintaining a functional occlusal surface is shown for cervid cheek teeth. Our results suggest that dentinal structure (in addition to enamel structure) should be taken more into consideration when discussing occlusal surface morphology in herbi- and omnivores from a functional point of view.

Animals↗
PubMed2004

Efficacy of different whitening modalities on bovine enamel and dentin.

Previous studies have shown that bleaching treatment may be efficient in both enamel and dentin, but it is still unknown how much the subsurface dentin contributes to the color change of teeth. This in vitro study evaluated the whitening effect of different external bleaching agents on enamel-dentin slabs and subsurface dentin. Ninety bovine teeth were distributed among six groups (A, Opalescence 10%; B, Opalescence PF 15%; C, Opalescence Quick; D, Opalescence Extra Boost; E, Rapid White; F, Whitestrips). Two enamel-dentin specimens were prepared from the labial surface of each teeth. In one of the specimens enamel was removed, resulting in a dentin (CD) disc of 1 mm high. The labial and the pulpal sides of the second specimen were ground until the remaining enamel and dentin layers of the enamel-dentin sample (ED) were 1 mm each. Whitening treatment of the ED specimens was performed according to manufacturers' instructions. Pre- and posttreatment Lab values of ED samples were analyzed using CIE-Lab. Baseline Lab values of dentin were analyzed by evaluation of the CD specimen. Finally, enamel of the ED specimens was removed and color change of the exposed dentin (D) was recorded. For all treatment agents significant color changes (DeltaE) were observed for enamel-dentin samples and subsurface dentin specimens compared to controls. In groups A-D DeltaE was significantly higher in dentin than enamel-dentin. Furthermore, L and b values of bleached enamel-dentin and subsurface dentin samples differed significantly from baseline. Treatment with the tested external whitening bleaching agents resulted in color change of both enamel-dentin and subsurface dentin samples. The results indicate that color change of treated teeth might be highly influenced by color change of the subsurface dentin.

Animals↗