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Transmission ratio distortion in the human body louse, Pediculus humanus (Insecta: Phthiraptera).

We studied inheritance at three microsatellite loci in eight F, and two F2 families of the body (clothes) louse of humans, Pediculus humanus. The alleles of heterozygous female-parents were always inherited in a Mendelian fashion in these families. Alleles from heterozygous male-parents, however, were inherited in two different ways: (i) in a Mendelian fashion and (ii) in a non-Mendelian fashion, where males passed to their offspring only one of their two alleles, that is, 100% nonrandom transmission. In male body lice, where there was non-Mendelian inheritance, the paternally inherited set of alleles was eliminated. We interpret this pattern of inheritance as evidence for extreme transmission ratio distortion of paternal alleles in this species.

Alleles↗

Meiotic pairing of sex chromosome fragments and its relation to atypical transmission of a sex-linked marker in Ephestia kuehniella (Insecta: Lepidoptera).

The physical basis of non-Mendelian segregation of a sex-linked marker was studied in sex- chromosome mutant females of eight ASF ('abnormal segregating females') lines in the flour moth, Ephestia kuehniella. Electron microscopical analysis of microspread synaptonemal complexes revealed that in one line, the Z chromosome segment that contained the dz+ allele was translocated onto an autosome. The resulting quadrivalent visible in early female meiosis was 'corrected' into two bivalents in later stages. This explains autosomal inheritance of the sex chromosome marker in this strain. In the other seven ASF lines, the type of meiotic pairing of an additional fragment (Zdz+) of the Z chromosome was responsible for abnormal segregation of the marker gene. In several of these lines, Zdz+ contained a piece of the W chromosome in addition to the Z segment, as was confirmed by comparative genomic hybridization (CGH). Zdz+ formed three alternative pairing configurations with the original sex chromosomes: (i) a WZZdz+ trivalent, (ii) a WZ bivalent and a Zdz+ univalent or (iii) a ZZdz+ bivalent and a W univalent. In the most frequent WZZdz+ configuration, Zdz+ synapsed with Z and, consequently, segregated with W, simulating W linkage. This explains the predominant occurrence of the parental phenotypes in the progeny. Zdz+ univalents or W univalents, on the other hand, segregated randomly, resulting in both parental and nonparental phenotypes. In two of these lines, the Zdz+ was transmitted only to females. The results suggest that the W chromosome segment in Zdz+ of these lines contains a male-killing factor which makes it incompatible with male development. Our data provide direct evidence for the regular transmission of radiation-induced fragments from lepidopteran chromosomes through more than 50 generations. This is facilitated by the holokinetic nature of lepidopteran chromosomes. We conclude that Zdz+ fragments may persist as long as they possess active kinetochore elements.

Animals↗

Louse (Insecta: Phthiraptera) mitochondrial 12S rRNA secondary structure is highly variable.

Lice are ectoparasitic insects hosted by birds and mammals. Mitochondrial 12S rRNA sequences obtained from lice show considerable length variation and are very difficult to align. We show that the louse 12S rRNA domain III secondary structure displays considerable variation compared to other insects, in both the shape and number of stems and loops. Phylogenetic trees constructed from tree edit distances between louse 12S rRNA structures do not closely resemble trees constructed from sequence data, suggesting that at least some of this structural variation has arisen independently in different louse lineages. Taken together with previous work on mitochondrial gene order and elevated rates of substitution in louse mitochondrial sequences, the structural variation in louse 12S rRNA confirms the highly distinctive nature of molecular evolution in these insects.

Animals↗

[Parasitism of Ips sexdentatus (Insecta: Scolytidae) by Parasitorhabditis ipsophila (Nematoda: Rhabditidae)].

The study of parasitism percentages and contaminations intensity in Ips sexdentatus parasitized by P. ipsophila as well as the examination of the wormholes in the galleries of the bark beetle gave better insight into certain features of nematode biology. Larvae of I. sexdentatus could be infected, whereas pupae could not. Adults were contaminated from initial stages of maturation and throughout preswarming maturation. Following swarming and installation on a new tree, insects were rapidly decontaminated, but recontamination could occur by the end of oviposition. P. ipsophila larvae were found in the mesenteron before they penetrated into the hind gut. Seemingly, the parasite underwent no evolution within its host. All developmental stages of the nematode could be observed in the galleries of the bark beetle as long as the latter was present. No apparent relation exists between parasitism of the digestive tract by P. ipsophila and parasitism of the body or fat body by Parasitaphelenchus or Contortylenchus diplogaster. P. ipsophila exerts very limited effects on I. sexdentatus populations. A slight delay in swarming and initiation of oviposition, and a very low decrease in density of notches of oviposition and of eggs was observed, but the features of the gallery of oviposition (total length, length before the first notch) showed no alteration. No mortality was detected.

Animals↗

[Fleas from the Neuguén Province (Argentina); description of 4 new taxa (Insecta, Siphonaptera)].

The collect of 973 Fleas on various small terrestrial mammals, from December 1987 till May 1988, in the Neuquén Province (Argentina), has given 20 taxa. Hectopsylla pascuali n. sp., Plocopsylla consobrina n. sp., Agastopsylla boxi gibbosa n. ssp., Chiliopsylla allophyla tonnii n. ssp. are described. Plocopsylla diana Beaucournu, Gallardo and Launay, 1986, Ctenoparia inopinata Rothschild, 1909, Neotyphloceras crassispina chilensis Jordan, 1936, Tetrapsyllus satyrus Beaucournu and Torrés-Mura, 1986, Ectinorus martini Lewis, 1976, are new from Argentina.

Animals↗

[The plague in Vietnam: history and inventory of collected fleas (insecta, Siphonaptera) in the inhabited zones].

Ten flea species are reported in anthropic zones of Vietnam. Xenopsylla vexabilis is also here included because of it has been involved in others plague's countries. Lentistivalius klossi is the only selvatic flea known as parasite of synanthropic rats. L. klossi bispiniformis (Li and Wang, 1958), first describe from Chinese specimens, is here synonymized (syn. nov.) with the nominal subspecies.

Animals↗

First report of Siphonaptera infesting Microtus (Microtus) cabrerae (Rodentia-Muridae-Arvicolinae) in Cuenca, Spain and notes about the morphologic variability of Ctenophthalmus (Ctenophthalmus) apertus personatus (Insecta-Siphonaptera-Ctenophthalmidae).

The fleas infesting Microtus (Microtus) cabrerae from three different areas of Cuenca province (Spain) have been studied. It is the first time that on ectoparasitological study of this badly known rodent has been done. Four Siphonaptera species have been detected: Rhadinopsylla (Actenophthalmus) pentacantha, Peromyscopsylla spectabilis spectabilis, Nosopsyllus fasciatus and Ctenophthalmus (Ctenophthalmus) apertus personatus, which was the most abundant species (26 males and 31 females of a total of 28 males and 35 females). Considering the great morphologic variability within the male processus basimerus ventralis (p.b.v.) of segment IX of C. personatus subspecies, three morphotypes have been recognised. The male polymorphism detected, would be the result of both host confinement and genetic selection acting on the parasite. It should be pointed out that C. (C.) apertus personatus is not narrowly host-specific, therefore further studies are required to clarify this taxonomic situation.

Animals↗

Differential diagnosis between Tunga penetrans (L., 1758) and T. trimamillata Pampiglione et al., 2002 (Insecta, Siphonaptera), the two species of the genus Tunga parasitic in man.

Of the ten currently known species of sand fleas, only two, Tunga penetrans and Tunga trimamillata, are known to be parasites of man, besides other warm blooded animals, most of which are peridomestic. The hosts of the other eight are limited to a few genera of wild mammals. T. trimamillata was only recently identified and differentiated from T. penetrans by features of the gravid female phase. In the present paper the different morphological characters of both for non-gravid females and males of the two species are described. In the non-gravid-females the distinguishing characters are: a) differences in the length of the first two segments of the maxillary palps (the first is the longest in T. trimamillata, whereas the second is the longest in T. penetrans), this feature is associated with another character i.e. the presence of short, thick spines in addition to the bristles on the surface of the segments only in T. trimamillata; b) the presence, only in T. trimamillata, of a row of spines on the antero-medial surface of the libia of the 3rd pair of legs; c) the last abdominal spiracle protrudes in T. trimamillata but not in T. penetrans; d) the hood of the hilla in spermatheca is surrounded by a papilla only in T. penetrans. The following morphological characters differentiate males of T. trimamillata and T. penetrans: a) T. trimamillata has a row of spines on the antero-medial surface of the tibia of the 3rd pair of legs; b) the diameter of the abdominal spiracles of T. trimamillata is smaller than that of T. penetrans and the edges of the spiracles are more regular; c) the claspers and aedeagus of T. trimamillata are shorter than those of T. penetrans; d) the profile of the oedeagal apodeme differs between the two species.

Animals↗

Leptopsyllines from Madagascar (Insecta: Siphonaptera: Ceratophyllidae): description of five new species of Paractenopsyllus Wagner, 1938.

Five new species of the malagasy endemic genus Paractenopsyllus Wagner, 1938, are described. Paractenopsyllus rouxi, P. ratovonjatoi, P. duplantieri, P. juliamarinus and P. gemelli have been collected on endemic small mammals and the black rat. Biotopes are mid-elevation rain-forests of the central highlands or the northern mountains of Madagascar. Morphological affinities between these new species and those already known provide taxonomic links within the genus. Differences in the spatial and altitudinal distributions and host ranges allow discussion of the potential factors important in the species distribution of this genus.

Altitude↗

[Two new species of fleas from Chile and Argentina(Insecta-Siphonaptera: Stephanocircidae and Rhopalopsyllidae) and raising of a new sub-genus of Plocopsylla Jordan, 1931].

A new subgenus of Plocopsylla is raised, Schrammapsylla Beaucournu and Ménier from the entire group B of Schramm & Lewis, 1988 and two new fleas from Chile and Argentina are described: Plocopsylla (S.) muruai n. sp. Beaucournu and Gallardo and Ectinorus (E.) mimacydis n. sp. Beaucournu and Gallardo. These two taxa are only known by the male sex.

Animals↗

[Three new species of the genus Medwayella Traub, 1972 (Insecta: Siphonaptera: Pygiopsyllidae) from Sabah (eastern Malaysia, Borneo)].

Medwayella traubiana n. sp., M. pfeifferi n. sp. and M. sabahae n. sp. (Pygiopsyllidae) are described from Sabah (north of Borneo island), the first two on Tupaia tana (Scandentia), the last on Sundasciurus lowii (Rodentia). Sex male is only identified, because these fleas have been collected in sympatry, or even in syntopy. Their determination is based on segment IX and aedeagus. If M. traubiana and M. pfeifferi are related to some known species, M. sabahae is clearly distinct from other Medwayella.

Animals↗

[Bird fleas (Insecta: Siphonaptera): taxonomic diversity, biogeographical distribution].

If Mammals are the primary hosts of Siphonaptera, 6% of them have changed their trophic appetency for Birds. What are the reasons, what are the adaptations to be adopted by Fleas, what are the families or species groups of fleas concerned, and at last what are the host-families? As to this last question, it is clear that deviation was ecological but not phyletical.

Adaptation, Physiological↗

Molecular characterization of Tunga trimamillata and T. penetrans (Insecta, Siphonaptera, Tungidae): taxonomy and genetic variability.

A new species of the genus Tungo, T. trimamillata has recently been described on the basis of several morphological traits. To explore the taxonomic status of this flea with respect to T. penetrans, we undertook a molecular analysis of cytochrome oxydase II and 16S rDNA mitochondrial genes and of the internal transcribed spacer 2 nuclear marker on samples of both species. Maximum Parsimony evaluations of the three data set indicate a differentiation compatible with a specific rank between the two fleas with very high levels of divergence. Both mitochondrial and nuclear data are in line with a recent bottleneck in the Malagasy population of T. penetrans, possibly due to the recent colonisation of Africa via human transportation. Further, significantly lower mitochondrial variability in the Ecuadorian populations of T. penetrans with respect to the T. trimamillata ones is also evidenced.

Amino Acid Sequence↗

[Macrostylophora kinabaluae n. sp. (Insecta: Siphonaptera: Ceratophyllidae), a new flea from Sabah (Borneo, Eastern Malaysia)].

This species, known only by a single male, is described from Mount Kinabalu and thus is recorded from the same area as Macrostylophora borneensis (Jordan, 1926), teste Traub (1972). It is distinguished from its congeneric and characterized by the absence of eriged setae on the thorax and first abdominal tergits, as well as by the shape of terminal segments and the phallosom. Macrostylophora kinabaluae was found to parasitize the Sciurid rodent Callosciurus prevostii that is widespread throughout most areas in the Malaysian subregion.

Animals↗

Anatomy of Tunga trimamillata Pampiglione et al., 2002 (Insecta, Siphonaptera, Tungidae) and developmental phases of the gravid female.

This paper deals with some internal anatomical features observed in histological sections and freshly dissected mounts of Tunga trimamillata, a Siphonaptera recently discovered in Andean regions of Ecuador from several mammals, including man. It was possible to study in males and also non-gravid and gravid females, the location and anatomy of several organs not previously described for this species: the testes, epididymis, ganglia, Malpighian tubules, eyes, rectal ampulla with one of its pads and structures which could be interpreted as midgut diverticula, whose presence has not been recorded in the Siphonaptera. The process of neosomy in the female during pregnancy is illustrated by photographs of the consecutive developmental phases, taken at the stereomicroscope. Furthermore, some details of the exoskeleton, spermatheca during different phases of pregnancy of the gravid female and the presence of a foreign body (parasite?) within the haemocoel have been displayed in specimens cleared with Hoyer's medium.

Animals↗

Secretion of brochosomes during the ontogenesis of a leafhopper, Oncometopia orbona (F.) (Insecta, Homoptera, Cicadellidae).

Secretion of brochosomes, intricately structured symmetrical protein-lipid particles produced in the specialized Malpighian tubules of the Cicadellidae, was studied during the ontogenesis of Oncometopia orbona (F.). Unlike most other cicadellids, O. orbona displays sexual dimorphism in the production of brochosomes. The Malpighian tubule secretory cells of nymphs, males and young females produce spherical brochosomes of 0.3-1.4 microm in diameter, extruded after molts through the hindgut and spread across the integument as a coating. In females with developing eggs the same cells switch to producing rod-like brochosomes of 3.5-11.0 microm in length, which are used to powder the oviposition sites. The transition involves a dramatic change in morphogenesis of the secretory particles. Both types of brochosomes derive from homogenous condensing Golgi granules and acquire their definitive structure as a result of growth, differentiation of the contents into a wall and a core, and development of a lattice of surface invaginations. Final stages of this process take place in Golgi-derived vacuoles. In the development of rod-like brochosomes the condensing granules coalesce into masses several times larger than nascent spherical type. These giant granules flatten, then become doughnut-shaped and finally break open into a pair of linear particles. Whether the chemical composition differs between the two types of brochosomes remains unknown.

Animals↗

The serosa of Manduca sexta (Insecta, Lepidoptera): ontogeny, secretory activity, structural changes, and functional considerations.

In Manduca sexta, the blastoderm forms successively and becomes immediately cellularized as the cleavage energids reach the surface of the oocyte. Presumptive serosal cells are large and contain 2 or 4 large polyploid nuclei; presumptive embryonic cells are small and mononuclear. All parts of the blastoderm participate in the uptake and digestion of yolk material. About 10 h post-oviposition, the blastoderm breaks at the amnioserosal fold and the extraembryonic part closes above the germ band and constitutes the serosa (12 h post-oviposition, i.e. 10% development completed). At once, the serosa starts to secrete a cuticle consisting of an epi- and a lamellated endocuticle. Detachment of the serosal cuticle, 22h post-oviposition, is reminiscent of apolysis of larval cuticle. Thereafter, the serosa deposits a membranous structure, the serosal membrane. The sercretory process lasts from 23h to 44h post-oviposition. At first a fine granular layer, then an amorphous, spongy-like, fibrillar layer is secreted via microvilli. This persisting membrane is tough, rubbery and very elastic. It may serve to bolster the serosa during katatrepsis (48h post-oviposition) and later embryonic movements. After detachment of the serosal membrane, 44h post-oviposition, a distinct subcellular reorganization of the serosa takes place. The nuclei become still larger and more irregular. Uptake of yolk granules, but not of lipid droplets, ceases, although interaction of serosa and yolk cells are intense. Serosal cells include many mitochondria, large areas of rER, besides some sER, increasing amounts of lysosomal bodies and prominent Golgi complexes. Most conspicuous is the assembly of spindle-shaped, electron-lucent vesicles below the apical surface. These vesicles may contain metabolic products which are released into the peripheral space. The studies show that the serosa assumes changing functions during embryogenesis: digestion of yolk substances, synthesis of a serosal cuticle and a serosal membrane, which may have a protective function, and excretion.

Animals↗