PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Morphological Characterization”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 397 records · Page 22Linked to original sources
PubMedDate not supplied

Tissue culture of normal rat glomeruli. Isolation and morphological characterization of two homogeneous cell lines.

Glomeruli were isolated from rat kidneys using enzymatic digestion, sieving and differential centrifugation. They were cultured in RPMI medium containing 15 per cent decomplemented fetal bovine serum. Using 3H-thymidine, two peaks of cell division occurred: the first was observed after 2 days in culture and reached its maximum on day 6; the second peak, starting on day 12, reached its maximum on day 22. Two cell types were identified by phase contrast, immunofluorescent and transmission electron microscopy. The first peak contained epithelial cells until day 6, while the second contained smooth muscle-like (mesangial) cells on day 30. The difference in the growth capacity of epithelial and smooth muscle-like cells allows their separate isolation by performing subcultures on day 6 for the former cells and on day 30 for the latter.

Animals↗
PubMed1977

[Position and problems is morphological characterizing of aged cells and organs (author's transl)].

Cellular changes in the process of aging are non-specific. A combination of nuclear inclusions, invaginations of the nuclear membrane, a reduction of the number of mitochondria with a simultaneous increase in size to the point of development of megamitochondria, a disorganization of the endoplasmic reticulum and a decrease of its surface density, an increase of the lysosomal volume share and a lipofucin accumulation indicates the existance of an aged cell. On the macroscopical and microscopical levels a diagnosis on the age of an organ is impossible.

Adult↗
PubMed1984

A morphological characterization of melanoma tumor cell interaction with a basement membrane model in the absence and presence of an anticancer agent.

In order to study the invasive characteristics of a patient-derived malignant melanoma cell line (C8146A), interactions of the tumor cells with a basement membrane model were studied in vitro with transmission and scanning electron microscopy. Tumor cells were cultured on amniotic basement membranes for 1, 2, and 3 day intervals and exposed to the anticancer agent Actinomycin D (Act D). Localization of the subcellular sites of drug-cell interaction was performed with protein A sepharose-purified rabbit anti-Act D IgG and immunofluorescence microscopy. The action of the rRNA inhibitor prevented a significant number of C8146A cells from invading the membrane as compared with the untreated cells. These data support the utilization of Act D as an anticancer agent, but pose several considerations regarding the mechanism of anti-inhibitory activity.

Antineoplastic Agents↗
PubMed1980

Morphological characterization of the erythrocyte membrane related to myxovirus receptors.

The receptor of myxoviral hemagglutinins is defined by terminal sialic acid residues of the major sialoglycoprotein (glycophorin) of the human erythrocyte membrane. A number of lectins and antibodies are suitable to specifically label this molecule. The proposed association of glycophorin with the structures revealed as intramembrane particles (IMP) on freeze-fractured membranes rests only on indirect evidence; IMP are not composed of glycophorin alone but glycophorin may be a component of them in association with other integral membrane proteins and/or lipids. Mapping experiments employing limulin, a lectin specific for sialic acids, and anti-glycophorin are described. These experiments demonstrate a lack of association of glycophorin and the IMP.

Erythrocyte Membrane↗
PubMed1983

Physicochemical and morphological characterization of a new allotransplantable mammary carcinoma ascites subline, TA3-St/ticol/-A.

The TA3-St/ticol ascites cell (I), immunoselected from the strain-specific TA3-St mammary carcinoma ascites cell of the strain A mouse for decreased H-2a antibody-binding capacity, underwent a spontaneous transition in vivo to a new cell line, TA3-St/ticol/-A (II). Line II was more allotransplantable than was the parental line (line I), and its absorptive capacity for anti-H-2a antibody was manyfold less than line I. Disruption of line II cells by lyophilization did not increase the absorptive capacity, in contrast to its marked enhancement in the TA3-Ha ascites cell under similar conditions. An explanation for the enhanced allotransplantability of line II may be related to either a loss of H-2a antigens or altered macromolecular structures at the cell surface: sialic acid, consisting of 93% N-glycolylneuraminic acid for line II and 9% for line I; altered chemical structures of cell surface glycoproteins, particularly a high-molecular-weight glycoprotein present in much greater proportion in line II than in line I; a macromolecular complex released by a protease from line I, but not line II; and I being agglutinable by concanavalin A, but not line II. Electron microscopy showed line II to be more pleomorphic and less rounded than was line I. Under high-resolution electron microscopy, the cell surfaces of both allotransplantable cells, lines II and I, exhibited thin filamentous material, material not observed at the surface of the nonallotransplantable TA3-St ascites cell.

Animals↗