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PubMed2005

Temperature-responsive cross-linked poly(epsilon-caprolactone) membrane that functions near body temperature.

The objective of this study is to develop a sensitive temperature-responsive material that would function near body temperature. To achieve this purpose, we compounded 2-branched and 4-branched poly(epsilon-caprolactone) macromonomers to modulate the transition temperatures of the resulting cross-linked materials. The temperature-responsive properties were studied using differential scanning calorimetry and X-ray diffraction measurements. As a result, the mixing ratios of each macromonomer or the total macromonomer concentrations were very dominant in modulating the transition temperatures. The materials could successfully control the permeation of the model drug, prednisolone, near body temperature.

Anti-Inflammatory Agents↗

PubMed2000

Ion exchange on resins with temperature-responsive selectivity III. Influence of complex formation stoichiometry on temperature dependence of resin selectivity.

The influence of temperature (293 308 K 1:1 IDA:Ni2+ complex is dominating. The Cu2+-Ni2+ exchange equilibrium from sulfate medium is characterized by the formation of nickel complexes of both stoichiometries within the whole temperature range studied. The dependence of alpha on T in Zn2+-Co2+ exchange system has been shown to be weaker than that in the Cu2+-Ni2+ system. This result is in a good agreement with the predictions made in the first communication of this series. The results of thermostripping experiments carried out for Cu2+-Co2+ exchange have shown that the efficiency of the thermostripping process depends on both the interval of working temperatures (deltaT) and its position on the temperature scale. The efficiency of thermostripping rises with an absolute deltaT value and also increases following the shift of temperature interval to the lower temperature range.

Hydrogen-Ion Concentration↗

PubMed2002

Temperature dependence of Henry's law constant in an extended temperature range.

The Henry's law constants H for chloroform, 1,1-dichloroethane, 1,2-dichloropropane, trichloroethene, chlorobenzene, benzene and toluene were determined by the EPICS-SPME technique (equilibrium partitioning in closed systems--solid phase microextraction) in the temperature range 275-343 K. The curvature observed in the ln H vs. 1/T plot was due to the temperature dependence of the change in enthalpy delta H0 during the transfer of 1 mol solute from the aqueous solution to the gas phase. The nonlinearity of the plot was explained by means of a thermodynamic model which involves the temperature dependence of delta H0 of the compounds and the thermal expansion of water in the three-parameter equation ln (H rho TT) = A2/T + BTB + C2, where rho T is the density of water at temperature T, TB = ln(T/298) + (298-T)/T, A2 = -delta H298(0)/R, delta H298(0) is the delta H0 value at 298 K, B = delta Cp0/R, and C2 is a constant. delta Cp0 is the molar heat capacity change in volatilization from the aqueous solution. A statistical comparison of the two models demonstrates the superiority of the three-parameter equation over the two-parameter one ln H vs. 1/T). The new, three-parameter equation allows a more accurate description of the temperature dependence of H, and of the solubility of volatile organic compounds in water at higher temperatures.

Organic Chemicals↗

PubMed2003

Temperature dependency and temperature compensation in a model of yeast glycolytic oscillations.

Temperature sensitivities and conditions for temperature compensation have been investigated in a model for yeast glycolytic oscillations. The model can quantitatively simulate the experimental observation that the period length of glycolytic oscillations decreases with increasing temperature. Temperature compensation is studied by using control coefficients describing the effect of rate constants on oscillatory frequencies. Temperature compensation of the oscillatory period is observed when the positive contributions to the sum of products between control coefficients and activation energies balance the corresponding sum of the negative contributions. The calculations suggest that by changing the activation energies for one or several of the processes, i.e. by mutations, it could be possible to obtain temperature compensation in the yeast glycolytic oscillator.

Biological Clocks↗

PubMed1998

Superoxide anion radical generation as a temperature stress response in the gills of freshwater catfish Heteropneustes fossilis: role in mucus exudation under elevated temperature.

Temperature induced superoxide anion radical (O2-) generation in vivo has been demonstrated in the gills of Heteropneustes fossilis by electron spin resonance (ESR) spin trapping. Temperature exposures from 25 degrees C to 37 degrees C for various times (1-4 hr) caused generation of O2- in the gill. The acid mucopolysaccharide test was conducted in gill sections during elevated temperatures. The results showed an increased activity of mucopolysaccharide in gills which indicate an increased mucus secretion in gills during elevated temperatures. The detectable stable levels of O2- in the gill at 32 and 37 degrees C temperature exposures point towards a probable role for this radical in the exudation of mucus under elevated temperature.

Animals↗

PubMed1995

Temperature-controlled high-performance liquid chromatography using a uniformly sized temperature-responsive polymer-based packing material.

Uniformly sized polymer-based packing materials modified with a temperature-responsive polymeric selector, poly(N-isopropylacrylamide), on either the internal or the external surface were prepared by an in situ surface-selective modification method. These packing materials were utilized as stationary phases in reversed-phase high-performance liquid chromatography to evaluate their ability to thermally control chromatographic separation selectivity. A packing material having a modified internal surface showed temperature-dependent separation selectivity in drug separation. Drugs that could not be separated below the phase transition temperature of the poly(N-isopropylacrylamide) selector were separated faster and with much better resolution above the phase transition temperature, while the unmodified base packing material afforded worse resolution of the drugs above the phase transition temperature due to the peak overlapping resulting from faster elution of all the solutes employed. In addition, a packing material modified on the external surface showed a temperature-responsive shift of the elution region for a polypeptide such as BSA, which was thought to be useful in direct injection of a serum sample.

Acrylic Resins↗

PubMed2005

Temperature alternation by an on-chip microheater to reveal enzymatic activity of beta-galactosidase at high temperatures.

A method to measure enzymatic activity at high temperatures by rapid temperature alternation of a microreactor with a microheater is proposed. On-chip microreactor and microheater were integrated on a glass plate by MEMS technology; this microheater can control the temperature of the microreactor with a response speed of 34.2 and 31.5 K/s for temperature rise and fall, respectively, with an accuracy of 3 degrees C. The enzyme, beta-galactosidase, was revealed to survive short exposure (4-s pulses) to temperatures above that which would "normally" denature them. Its activity at 60 degrees C was revealed to be approximately 4 times greater than that at room temperature. This method not only gives new kinetic information in biochemistry but also enables application in highly sensitive biosensors.

Hot Temperature↗

PubMedDate not supplied

Temperature-responsive bioconjugates. 2. Molecular design for temperature-modulated bioseparations.

We have synthesized carboxyl semitelechelic oligo(N-isopropylacrylamide) (OIPAAm) using radical telomerization with 3-mercaptopropionic acid. This telomerization is also effective for the synthesis of carboxyl semitelechelic co-oligomers of IPAAm with butyl methacrylate (BMA) as hydrophobic or N,N-dimethylacrylamide (DMAAm) as hydrophilic comonomers. All co-oligomers are highly water-soluble at lower temperatures and exhibit phase separation with increasing temperature. Pure OIPAAm exhibits a lower critical solution temperature (LCST) at 32 degrees C, and the LCST for co-oligomers can be controlled to increase over 32 degrees C with increasing DMAAm composition and to decrease below 32 degrees C with increasing BMA composition. OIPAAm was grafted to bovine serum albumin (BSA) and bovine plasma fibrinogen (BPF) by activated ester-amine coupling. These OIPAAm-biomolecule conjugates maintain their temperature responses, are soluble in cold water, and precipitate over a range of temperatures related to oligomer content. Conjugates could be selectively precipitated and independently separated from conjugate solution mixtures with increasing temperature. In this case, the number of OIPAAm molecules attached to a conjugate affects the aggregate sizes of precipitated conjugates in mixtures. Both conjugate mixture ratios and solution concentrations influence the contamination of oligo(IPAAm-co-DMAAm)-BSA conjugates in precipitated oligo(IPAAm-co-BMA)-BPF conjugates. Furthermore, precipitated conjugates separated using centrifugation and filtration redissolve in water and maintain their biofunctionality, indicating the potential of strategy in reversible bioreactors and protein separations.

3-Mercaptopropionic Acid↗

PubMedDate not supplied

Temperature-responsive bioconjugates. 3. Antibody-poly (N-isopropylacrylamide) conjugates for temperature-modulated precipitations and affinity bioseparations.

Immunoglobulin G (IgG) has been modified by poly(N-isopropylacrylamide) (PIPAAm) to create a novel bioconjugate which exhibits reversible phase transition behavior at 32 degrees C in aqueous media. A terminal carboxyl group introduced into PIPAAm molecule by polymerization of IPAAm with 3-mercaptopropionic acid was used for conjugation to IgG via coupling reaction of activated ester with protein amino group. These conjugates exhibited rapid response to changes in solution temperature and significant phase separation above a critical solution temperature corresponding to that for the original PIPAAm. These conjugates bound to antigen quantitatively in aqueous system, and antigen-bound complex also demonstrated phase separation and precipitation above a critical temperature. Precipitate was reversibly redissolved in cold buffer. Though particular conjugate which includes 12 molecules of PIPAAm with 6,100 molecular weight suppressed more than 95% of Fc-dependent binding with protein A, it retained approximately 60% of original specific antigen binding activity. It was manifested that polymer content of conjugate was 20-30 wt% for the case of 6,100 molecular weight of PIPAAm to demonstrate specific antigen binding activity most effectively and to reduce Fc-dependent binding with protein A. IgG-PIPAAm conjugates were soluble in water and formed antigen-bound complex in homogeneous solution system below a critical temperature. These conjugates were separated from solution and other solutes corresponding to PIPAAm nature and scarcely bound to antigen above a critical temperature. It is revealed that temperature-responsive PIPAAm conjugated to biomolecule operated as a switching molecule. These phenomena are attractive for not only reversible bioreactors and protein separations but also carrier substrate to localize biomolecules such as drugs, peptides and hormones in a living body.

3-Mercaptopropionic Acid↗

PubMed1992

Effects of temperature on protein structure and dynamics: X-ray crystallographic studies of the protein ribonuclease-A at nine different temperatures from 98 to 320 K.

Structures using X-ray diffraction data collected to 1.5-A resolution have been determined for the protein ribonuclease-A at nine different temperatures ranging from 98 to 320 K. It is determined that the protein molecule expands slightly (0.4% per 100 K) with increasing temperature and that this expansion is linear. The expansion is due primarily to subtle repacking of the molecule, with exposed and mobile loop regions exhibiting the largest movements. Individual atomic Debye-Waller factors exhibit predominantly biphasic behavior, with a small positive slope at low temperatures and a larger positive slope at higher temperatures. The break in this curve occurs at a characteristic temperature of 180-200 K, perhaps indicative of fundamental changes in the dynamical structure of the surrounding protein solvent. The distribution of protein Debye-Waller factors is observed to broaden as well as shift to higher values as the temperature is increased.

Chemical Phenomena↗

PubMed2001

Usefulness of the Kohlrausch-Williams-Watts stretched exponential function to describe protein aggregation in lyophilized formulations and the temperature dependence near the glass transition temperature.

PURPOSE: We studied the feasibility of using the Kohlrausch-Williams-Watts stretched exponential function (KWW equation) to describe protein aggregation in lyophilized formulations during storage. Parameters representing "mean aggregation time" (taua) and stretched exponential constant (betaa) were calculated according to the KWW equation by assuming that the time required for protein molecules to aggregate (tau) varies because of the fact that protein aggregation occurs at a rate that depends on the degree of protein deformation resulting from stresses created during freeze-drying. The temperature dependence of the parameters near the glass transition temperature was examined to discuss the possibility of predicting protein aggregation by accelerated testing. METHODS: Protein aggregation in lyophilized bovine serum gamma-globulin (BGG) formulations containing dextran or methylcellulose, at temperatures ranging from 10 to 80 degrees C, was followed by size-exclusion chromatography. RESULTS: Non-exponential BGG aggregation in lyophilized formulations could be described by the KWW equation. The taua and betaa, parameters changed abruptly around the NMR relaxation-based critical mobility temperature for formulations containing dextran and methylcellulose. In the glassy state, in contrast, the taua parameter of these formulations exhibited continuous temperature dependence. The parameter taur, as calculated from taua, and betaa, reflected differences in tau values between the two excipients. CONCLUSIONS: The results indicate that the parameter betaa is reflective of physical changes wihtin lyophilized formulations. Within the temperature range, during which no abrupt changes in betaa were observed, knowledge regarding the taua and betaa parameters allows the rate of protein aggregation to be predicted. The parameter taur was found to be useful in comparing the protein aggregation behavior of formulations having different taua and betaa values.

Algorithms↗

PubMed1981

Rapid decrease in thymidine kinase activity of mouse cell temperature-sensitive mutants at a non-permissive temperature.

A rapid decrease in the incorporation of [3H]thymidine into DNA at a non-permissive temperature was observed in two temperature-sensitive mutants that were isolated from mouse FM3A cells. This change was not due to a decrease in the rate of DNA replication, but was closely associated with a decrease in thymidine kinase activity of these cells. Experiments to test thermolability of thymidine kinase in extracts showed that there are two components of the thymidine kinase, but there was no alteration in the sensitivity of the enzyme to high temperature. Also, the decrease in enzyme activity in the temperature-sensitive mutants at the non-permissive temperature occurred much faster than expected from the half-life of the enzyme in wild-type cells, which was measured in the presence of cycloheximide. These results suggested that the enzyme was somehow rapidly inactivated, or degraded, in the cells at the non-permissive temperature.

Animals↗

PubMed2003

Temperature-dependent, neurotrophic factor-elicited, neuronal differentiation in adrenal chromaffin cell line immortalized with temperature-sensitive SV40 T-antigen.

We established adrenal medullary cell lines from transgenic mice expressing an oncogene, the temperature-sensitive simian virus 40 large T-antigen, under the control of the tyrosine hydroxylase promoter. A clonal cell line, named tsAM5D, conditionally grew at a permissive temperature of 33 degrees C and exhibited the dopaminergic chromaffin cell phenotype as exemplified by the expression pattern of mRNA for catecholamine-synthesizing enzymes and secretory vesicle-associated proteins. tsAM5D cells proliferated at the permissive temperature in response to basic fibroblast growth factor (bFGF) and ciliary neurotrophic factor (CNTF). At a non-permissive temperature of 39 degrees C, bFGF and CNTF acted synergistically to differentiate tsAM5D cells into neuron-like cells. In addition, tsAM5D cells caused to differentiate by bFGF plus CNTF at 39 degrees C became dependent solely on nerve growth factor for their survival and showed markedly enhanced neurite outgrowth. In the presence of bFGF and CNTF, the morphological change induced by the temperature shift was associated with up-regulated expression of neuronal marker genes including neuron-specific enolase, growth-associated protein-43, microtubule-associated protein 2, neurofilament, and p75 neurotrophin receptor, indicating that the cells underwent neuronal differentiation. Thus, we demonstrated that tsAM5D cells could proliferate at permissive 33 degrees C, and also had the capacity to terminally differentiate into neuron-like cells in response to bFGF and CNTF when the oncogene was inactivated by shifting the temperature to non-permissive 39 degrees C. These results suggest that tsAM5D cells should be a good tool to allow a detailed study of mechanisms regulating neuronal differentiation.

Adrenal Gland Neoplasms↗

PubMed1973

A differential effect of heavy water on temperature-dependent and temperature-compensated aspects of circadian system of Drosophila pseudoobscura.

D(2)O is the only "chemical" agent that consistently affects the frequency of circadian oscillations: its effect is now known to be so widespread and predictable that its action merits closer study as a potential clue to the currently obscure concrete nature of circadian oscillators. The great diversity of D(2)O effects on biological systems in general is briefly reviewed and the need for rejectable hypotheses concerning the action of D(2)O on circadian clocks is stressed because current speculation on its action yields "predictions" expected from almost any hypothesis. We consider the hypothesis that it "diminishes the apparent temperature" of the cell and proceed to test this by examining the effect of D(2)O on temperature-dependent and temperature-compensated aspects of the circadian system in Drosophila. We find these components respond as differentially to D(2)O as they do to temperature; we conclude, however, with a warning that this result may be equivocal if, as we now suspect, the frequency of circadian oscillations is generally homeostatically conserved-not only in the face of temperature change, but change in any variable to which it is sensitive. More crucial tests of the temperature-equivalence hypothesis for D(2)O action are defined.

Animals↗

PubMed1996

Temperature dependence of the mitochondrial inner membrane anion channel. The relationship between temperature and inhibition by protons.

In this paper, we investigate the temperature and pH dependence of the mitochondrial inner membrane anion channel (IMAC) that is believed to be involved in mitochondrial volume homeostasis. At pH 7. 4, the flux of malonate is highly temperature-dependent with rates increasing from 1 nmol/min mg at 5 degrees C to 1900 nmol/min mg at 45 degrees C. The Arrhenius plot is nonlinear with the activation energy increasing from 21 kJ/mol (Q10 = 1.3) to 193 kJ/mol (Q10 = 13) as the temperature is decreased. This temperature dependence is unusual and not seen with solutes that are transported through the bilayer such as NH4OAc, malonamide, and KSCN (plus valinomycin) or even for cytochrome c oxidase-dependent uptake of potassium (plus valinomycin). The temperature dependence of IMAC is closely related to the inhibition of IMAC by protons. Thus, we find that the pIC50 for protons decreases from 9.3 (Hill coefficient = 1.0) at 5 degrees C to 7.1 (Hill coefficient = 2.5) at 45 degrees C. This behavior is explained on the basis of a new kinetic model for IMAC in which the net open probability is not only modulated by the binding of three protons but also by temperature via effects on the open probability of the unprotonated channel and the pK of one of the inhibitory protonation sites.

Animals↗

PubMed1996

Influence of temperature during growth on responses of hens to high or low temperatures during lay.

1. Commercial pullets were grown at cool (10 degrees -20 degrees C) or hot (25 degrees -35 degrees C) temperatures to similar bodyweights at 18 weeks of age. Between 18 and 50 weeks the birds were either kept at the same temperatures as during growth or transferred to the alternate temperature. 2. Birds kept at the cool temperatures throughout life ate most food and gave the best production during lay. Minimum food intake and poorest performance were obtained with birds kept at the hot temperatures throughout life. 3. Performance in the hot environment during lay was improved by rearing birds in the cool environment, the response being related to an increased food intake. Food intake in the cool environment during lay was reduced, with only minor effects on performance, in birds which had been reared in the hot environment. 4. The results of the present study show that production responses during lay are affected by the temperatures experienced by hens during both growth and lay.

Animal Feed↗

PubMedDate not supplied

Chill-coma temperature in Drosophila: effects of developmental temperature, latitude, and phylogeny.

We modify and apply a nonlethal technique for rapidly quantifying the cold tolerance of large numbers of Drosophila and other small insects. Flies are transferred to individual vials, cooled in groups in progressive 0.5 degrees C steps, and checked for loss of righting response (chill-coma temperature [T(cc)]). Flies recover quickly when transferred to ambient temperature, and thus this technique potentially can be used in selection experiments. We applied this technique in several experiments. First, we examined the sensitivity of T(cc) to developmental temperature. Drosophila melanogaster (Congo, France), Drosophila subobscura (Spain, Denmark), and Drosophila ananassae (India) were reared from egg to adult at 15 degrees, 18 degrees, 25 degrees, or 29 degrees C, transferred to 15 degrees C for several days, and then progressively chilled: T(cc) was positively related to developmental temperature, inversely related to latitude of the population, but independent of sex. The sensitivity of T(cc) to developmental temperature (acclimation flexibility) was marked: T(cc) shifted on average 1 degrees for each 4 degrees C shift in developmental temperature. Among 15 species of the obscura group of Drosophila, T(cc) varied from -0.1 degrees to 4.5 degrees C; T(cc) was inversely related to latitude in both nonphylogenetic and phylogenetically based ANCOVA (standardized independent contrasts) and was unrelated to body size.

Acclimatization↗

PubMed1981

Prediction of oesophageal temperatures from core temperatures measured at other sites in man.

Transient changes in core temperature were induced in seven subjects by immersion in a hot, then a cold, bath and by light intermittent exercise. Measurements of core temperature were made at four sites: the external auditory canal, the oesophagus, the rectum and the gastrointestinal tract. A mathematical model was derived to enable prediction of oesophageal temperature from measurements made at any one of the other sites. The equations for auditory canal and rectal temperatures were very similar to those previously derived in the Institute of Aviation Medicine; use of an equation to predict oesophageal temperatures from gastrointestinal tract temperatures measured in the field using a radio-pill would therefore appear to be reasonable.

Aerospace Medicine↗