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PubMed1979

[Studies of bovine Mycoplasma mastitis. 3. Testing of biochemical properties of Mycoplasma strains isolated from 3 stocks].

Thirty-two isolates of altered milk samples taken from cows with mastitis on three industrialised dairy cattle units were biochemically tested following five passes through no-inhibitor media, assessment of filtratability through 450-nm-membrane filter, and clone assay. Something between five and ten clones of each of the strains involved were tested for their growth properties both at 22 degrees C and in no-serum media, sensitivity to digitonin and capability of aesculin hydrolysis. TTC reduction (triphenyltetrazolium-chloride), as well as for decomposition of glucose, arginine, and urea. The properties found were characteristic of M. bovis, A. laidlawii, and A. axanthum, and of the family of mycoplasmataceaea in the strains of one of the stocks.

Animals↗
PubMed1979

[Studies of bovine Mycoplasma mastitis. 4. Serological classification of Mycoplasma strains isolated from 3 stocks].

Thirty-two isolates from altered milk samples taken from cows with mastitis of two herds were serologically tested by the WHT against rabbit hyperimmune sera of 16 reference and type strains. The strains tested were associated and grouped in the following way: 16 M. bovis, nine A. laidlawii, and seven A. axanthum. The classification of on M. bovis strain was confirmed by SHT. Five isolates from another stock, with biochemical properties related to the family of mycoplasmataceae, were not serologically identified.

Animals↗
PubMed1979

[Studies of Mycoplasma mastitis in cattle. 5. Studies of udder pathogenicity of Mycoplasma and Acholeplasma strains of different origins].

Isolated acholeplasma laidlawii strains exhibited highly differentiated behaviours regarding their udder pathogenicity. Twelve of 16 tested strains were pathogenic to udder. Symptoms of acute udder inflammation were caused by all ten A. laidlawii strains isolated from differentiated material of calf, but by only two of six strains isolated from differentiated material of cattle. Intracisternal instillation of both strains from milk and one strain each from udder skin or cervical mucus caused merely temporary disorders of secretion. Ultrasonic extracts of A. laidlawii strains, some of them additionally heated, were intracisternally applied, as well. Udder irritation was caused only by those acholeplasma strains which were udder-patha was assumed to be attributable to a toxin of the polysaccharide type. Pathogenicity to udder was recorded also from one M. alkalescens strain isolated from a nose swab taken of cattle as well as from two A. granularum strains isolated from calf lungs.

Acholeplasma laidlawii↗