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Changes in bone mineral density of lumbar vertebrae after parturition in African green monkeys (Cercopithecus aethiops).

We examined the effect of lactation on the maternal bone components in laboratory-bred African green monkeys. Bone mineral density (BMD) with bone mineral content (BMC), bone area and mean width of lumbar vertebrae (L3-L5) were measured by dual-energy X-ray absorptiometry (DXA). Measurements were conducted at two-week intervals from parturition up to 40 weeks after parturition. The BMC and BMD values gradually decreased during the lactation period and then gradually increased after weaning, but the BMC and BMD of the maternal lumbar vertebrae did not recover completely to the values at parturition even at 40 weeks after parturition.

Absorptiometry, Photon↗

Changes in normal vaginal flora of African green monkeys (Cercopithecus aethiops) during the menstrual cycle.

Changes in normal vaginal flora of African green monkeys associated with the estrous cycle were examined by the swab method. Bacteroidaceae, corynebacteria, and streptococci were predominant throughout the whole estrous cycle, although individual differences were great. It was also clear that all bacterial species tended to decrease in the menstrual phase. Lactobacilli, the most predominant bacteria in the normal vagina of humans, were detected only in very low numbers in the African green monkey, suggesting that the normal vagina of the African green monkey has a different ecosystem from that of normal human vaginal flora.

Animals↗

[Age-related changes in bone mineral density, mean width and area of the lumbar vertebrae in male African green monkeys (Cercopithecus aethiops)].

We were able to measure bone mineral density (BMD), mean bone width and the area of the lumbar vertebrae in male African green monkeys with a dual-energy X-ray absorptiometry (DPX-alpha, Lunar, USA). For analysis of spinal bone mass, mean width and area of the bone, we scanned lumbar vertebrae L3-L5 using a pediatric analysis program. We used restraining bed made of formed polystyrol to support the animal in a supine position. The precision of the measuring technique was calculated by averaging the coefficients of variance of the 5 scans obtained in 5 animals aged from 5 to 7 years. The precision values for BMD, bone width and area were 1.9%, 0.9% and 2.6%, respectively. Furthermore, age-related changes in bone measurements were demonstrated using laboratory-bred males (n = 45) and, additionally, wild-originated males (n = 7, estimated ages over 15 years old). The values of B M D, bone width and area at 6 months old were about 0.3 g/cm2, 1.2cm and 4.5 cm2 respectively. These values increased with aging to about 0.7 g/cm2, 1.5 cm and 11cm2 at 5 years old, respectively, and were judged to reach plateau. However, animals over 10 years old and wild-originated animals which had been kept over 10 years in our breeding colony showed rather smaller BMD and area values than the animals aged 5 to 10 years. The animals aged between 5 and 10 years seemed to have retained a peak bone mass in this primate species.

Absorptiometry, Photon↗

[Relationship between serum biochemical measurements and spinal bone mineral density in African green monkeys (Cercopithecus aethiops)].

Spinal bone mineral density (BMD) of African green monkeys were measured by a dual-energy X-ray absorptiometry (DEXA). The value determined in male was rather higher (0.674 +/- 0.068g/cm2, Mean +/- S. D.) than that in female (0.611 +/- 0.105g/cm2), while the coefficient of variance was larger in female (17%) than in male (10%). Females were divided into two groups according to their diversity of BMD values. Serum biochemical values were compared among three groups, one male group (n = 37) and two groups of female (A: n = 31, B: n = 22), using a canonical discriminant analysis. As the result of the canonical discriminant analysis, closed relationship between serum biochemical properties and BMD was demonstrated. Lower BMD, lower serum protein concentration and higher serum inorganic phosphorus may be the reflection of malnutrition in this primate species. In conclusion, animals showing osteopenia were included in our breeding colony of the African green monkey judging by the value of bone measurements and serum biochemical measurements.

Absorptiometry, Photon↗

[Changes in bone mineral density of lumbar vertebrae during a six-month interval in African green monkeys (Cercopithecus aethiops)].

Changes in measurements of lumbar vertebrae (L3-L5) during a six-month interval in African green monkeys were demonstrated using dual-energy X-ray absorptiometry (DEXA, DPX-alpha, Lunar, USA). Area, mean width and bone mineral content (BMC) of L3-L5 were measured, and bone mineral density (BMD) was calculated using 62 laboratory-bred and 23 wild animals. Laboratory-bred animals were divided into 4 groups according to their gender and age, and in addition, two groups of wild animals were constructed according to gender. Although no remarkable change in BMD of laboratory-bred animals aged less than two years was observed at a six-month interval, significant decreases in BMD were detected in laboratory-bred animals aged over 6 years as well as in wild animals. Thus, DEXA seems to be useful for investigating the kinetics of BMD in aged animals during a relatively short period such as six-months.

Absorptiometry, Photon↗

A preliminary investigation on the gastrointestinal helminths of the Barbados green monkey, Cercopithecus aethiops sabaeus.

Faecal samples were collected from fifty three freshly captured monkeys which were kept at the Barbados Primate Research Centre and Wildlife Reserve (BPRCWR). Examination of these samples for gastrointestinal helminths using the zinc sulphate floatation method revealed an overall infection rate of 88.7%. The parasites observed included Strongyloides (62.4%), Physaloptera (58.5%), Trichuris (52.8%), Hookworm (34.0%), Oesophagostomum (30.2%), Trichostrongylus (3.8%) and Ascaris (5.7%). No significant differences in overall prevalence were observed according to sex or age. Polyparasitism appeared to be common as it was observed in 92.5% of all monkeys examined. It is concluded that these monkeys could act as reservoirs of some of the parasites which can infect man.

Animals↗

Operant conditioning of autogrooming in vervet monkeys: Cercopithecus aethiops.

Vervet monkeys received food reinforcement contingent on autogrooming. Experiment 1 reinforced grooming on a schedule of increasing intermittency and grooming increased in frequency and duration; with only pauses reinforced, grooming decreased in frequency and duration. Experiment 2 demonstrated differentiation of operant autogrooming; in each session a different single form of grooming was reinforced (for example, grooming the tail only), and that form increased in frequency while other forms became less frequent. In Experiment 3 scratching was succesfully conditioned with a method that selectively reinforced variety in behavior; reinforcement was contingent on a shift in scratching form. In Experiment 4, with no contingencies on grooming, a prefood stimulus did not increase autogrooming whether or not grooming had previously resulted in contingent reinforcement. The form of conditioned autogrooming resembled the form of unconditioned autogrooming. The discussion suggests how reinforcement principles can account for changes in the topography of operant behavior.

Journal Article↗

Changes in WGA-lectin binding sites on sperm during epididymal transit in the Chacma baboon (Papio ursinus) and the vervet monkey (Cercopithecus aethiops).

Epididymal maturation of sperm entails molecular changes to the sperm plasma membrane. These changes are reflected by changes in lectin binding. The wheat germ agglutinin (WGA) lectin was used, since it binds to acetylglucosamine and sialic acid, which are involved in sperm function. The objective was to study changes in WGA-lectin binding during epididymal passage and in the ejaculate of the Chacma baboon and vervet monkey. The intensity and location of lectin binding on sperm from the ejaculate and the caput, corpus, and cauda epididymidis of 5 adult males from each species were studied. In both baboon and monkey sperm, the acrosomal region had the highest intensity stain and the equatorial region had the lowest intensity. Significant differences in staining of the different regions of sperm were found for the different epididymal sites and the ejaculate of both species. It was concluded that changes in the molecular composition of the sperm membrane, taking place during epididymal maturation, can at least in part be monitored by studying WGA-lectin binding on sperm. The changes noted are probably important for the organization of a membrane of the correct molecular conformation and mandatory for complete sperm function.

Animals↗

Experimental louse-borne relapsing fever in the grivet monkey, Cercopithecus aethiops. III. Crisis following therapy.

Eight grivet monkeys infected with Borrelia recurrentis received tetracycline (12.5mg/kg body weight) on the 3rd or 4th day of spirochetemia. Leukopenia, fever, hyperpnea, and tachycardia developed within 2 hours as spirochete counts fell to undetectable levels. These events closely simulated the crisis in human louse-borne relapsing fever in both timing and extent.

Animals↗

Peripheral blood lymphocyte immunocompetence in wild African green monkeys (Cercopithecus aethiops) and the effects of capture and confinement.

Capture and prolonged confinement of wild African green monkeys (AGM, C. aethiops) in single housing have been shown to result in high morbidity and mortality. The present study was designed to analyse immune modulation associated with these procedures in AGMs. Four wild resting AGMs were stunned with a rifle shot as controls (group I). Seven newly captured monkeys (group II) and seven laboratory conditioned monkeys (group III) housed singly were anaesthetised with ketamine hydrochloride on day 18 and 45, and 180 and 210 post-capture, respectively. Heparinized blood (10 ml) was drawn from each animal and lymphocytes were cultured in six wells per sample. A triplicate subset was stimulated with 10 microliters Concanavalin A for 48 hours. The cells were pulsed with tritiated thymidine and harvested on filter discs 18 hours later. Lymphocyte stimulation indices (stimulated cell count-unstimulated cell count) were 3598 in group I. 3843 and 4395 in group II and 2809 and 2196 in group III, respectively. This indicates that AGMs confined to single housing for a prolonged period exhibit immune suppression. The results confirm our previous findings that the AGM is more susceptible to stress associated with immune suppression and subsequent high frequency of infectious diseases than other East African nonhuman primates confined to laboratory housing.

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Evaluation of immune associated cells in lesions of L. major infected vervet monkeys (Cercopithecus aethiops).

Vervet monkeys were used to characterize immune associated cell types recruited into lesion sites as a result of experimental primary and secondary infections with Leishmania major. A heavy cellular infiltration consisting primarily of CD8+ (cytotoxic/suppressor) T cells were observed in the lesions. A small number of B lymphocytes and NK cells were also stained. Changes in cell type populations observed in the lesions were similarly reflected in the draining lymph nodes. Studies from control sites in all the animals revealed the presence of CD8+ T cells both in the epidermis and dermal layers of the normal skin. B cells, CD16 (NK cells) and CD4 (helper T cells) positive cells were virtually absent in the normal skin. It was concluded that CD8+ T cells were the predominant cells in the lesions. It also appeared that similar cell types were restricting the parasites at the lesion site both in primary and secondary L. major infections in vervet monkeys.

Journal Article↗