Effect of rifampicin and free amino acids on replication of DNA in Lactobacillus acidophilus.
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Abdominal pain is common in the general population and, in patients with irritable bowel syndrome, is attributed to visceral hypersensitivity. We found that oral administration of specific Lactobacillus strains induced the expression of mu-opioid and cannabinoid receptors in intestinal epithelial cells, and mediated analgesic functions in the gut-similar to the effects of morphine. These results suggest that the microbiology of the intestinal tract influences our visceral perception, and suggest new approaches for the treatment of abdominal pain and irritable bowel syndrome.
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Withdrawal and subsequent restoration of deoxyriboside to a deoxyriboside-requiring mutant leads to synchronous cell division. There is a time lag between restoration and the first synchronous division; during this time the amount of deoxyribonucleic acid (DNA) per cell increases in stepwise fashion, and tnass per cell increases linearly with time. The onset of cell division is heralded by cessation of DNA synthesis and an abrupt increase in rate of mass synthesis.
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