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At least 433 records · Page 24Linked to original sources
PubMed1978

[Adaptation of the deoxyglucose method to the cellular level: histologic preparation of the central nervous system for high resolution radioautography].

Vascular perfusion of all products required for primary fixation, postfixation, dehydration and embedding of nervous tissue in Epon permits radio-autographic detection of radioactivity accumulated in the central nervous system after intravenous injection of [3H]deoxyglucose. This histological technique should allow application of the deoxyglucose method at cellular if not subcellular level, since a high proportion of the tracer appears to be retained in situ in specimens adequately preserved for light and electron microscope radio-autography.

Animals↗
PubMedDate not supplied

[Discovery of internal sources of the neural retinal regeneration after its detachment in Pleurodeles. II. The radioautography study].

We continued to study internal sources of neural retina regeneration in adult lower vertebrates. A radioautographic study carried out was aimed at the visualization of proliferating cells in different areas of the retina and at different times after its detachment in the newts Pleurodeles waltl. Analysis of serial standards and semithin sections has shown several types of cells capable of incorporating a labeled DNA synthesis precursor. The marker of proliferating cells was incorporated in the cells of Mullerian glia, pigment epithelium, and growth zone and in small neural cells of the vitreal part of the inner nuclear layer. A source of formation of neuroepithelial "insertions" responsible for restoration of the complete cell composition of the retina damaged as a result of detachment should be searched among these cell types. Neither bipolar cells with Landoldt's club in the inner nuclear layer, nor bipolar-like cells in the outer nuclear layer, which are a source of formation of additional photoreceptors, were found among the 3H-thymidine- labeled cells. A conclusion was drawn that the bipolar-like cells of P. waltl are postmitotic cells, that did not reach terminal differentiation.

Animals↗
PubMed1995

Morphometry in anatomy: image analysis on fine structure and histochemical reactions with special reference to radioautography.

Anatomy now has a step to transfer from qualitative science to quantitative science. The methods of anatomical investigation employ optical instruments. Anatomy initially described qualitative features of the structure of the body, but now it turns to transfer to new fields measuring the number, size, length, area and volume of not only, structure but also the histochemical components of the structure with quantitative analysis. In order to quantify histochemical reactions in various organs, light and electron microscopic radioautograms were prepared from the liver, pancreas, intestines and kidney which were labeled with 3H-thymidine, 3H-uridine, 3H-leucine, 3H-glucosamine and 3H-glycerol, fixed in glutaraldehyde and osmium tetroxide, embedded in Epoxy resin, sectioned either thick or thin, coated with either Konica NR-M2 or -H2 radioautographic emulsions, exposed, developed, stained for either light or electron microscopic radioautographs. The radioautographs were observed with Olympus Vanox AHB-LB or Nikon Optiphot-2 light microscopes or Hitachi H-700, JEOL JEM-4000EX electron microscopes at 200 and 400 kV. The radioautograms were analysed with various image and analysed such as PPA-250, MOP, Digigramer-G, IBAS-II, Quadra 900, Luzex 300 to count the number of silver grains and to measure the size and number of structures. Some electron microscopic radioautographs were analysed in analytical electron microscopes, combined with Hitachi H-700 Horiba EMAX-1800E, JEOL JEM-200CX Kevex 7000-77, and JEOL JEM-4000EX Tracor-Northern TN-5400 to quantify silver grain contents. The results demonstrated that correct data can be obtained by controlling both full-automatic and semi-automatic image analysers by operating them manually.

Anatomy↗