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Capsular injuries of the proximal interphalangeal joint.

Although capsular injuries of the PIP joints are common, their management is frequently complicated. Successful treatment must begin with a detailed history because reviewing the mechanism of injury may provide information relevant to the pathomechanics of the capsular disruption and facilitate making an accurate diagnosis. Grades I and II volar plate and collateral ligament sprains represent the vast majority of PIP joint injuries. They are best treated with a short period of dorsal splinting followed by supervised mobilization. Although splinting is also applicable for grade II sprains associated with instability and most grade III sprains, the initial period of immobilization should be longer. The prognosis for recovery is generally good, although some residual tenderness or joint stiffness are common complications. Dorsal capsular injuries, if unrecognized, result in deformity rather than instability. The majority of these injuries can also be treated by closed means, but they require more prolonged immobilization and more commonly result in reduced mobility than volar plate and collateral ligament injuries. Capsular injuries that are compound, irreducible, or associated with a large intraarticular fracture can result in serious problems. Frequently, these injuries require primary surgical treatment, particularly in the case of the irreducible dislocation, which always requires surgery. An exception to the generally poor prognosis of these injuries is the irreducible volar dislocation because the central tendon remains intact permitting early postoperative joint mobilization. A chronic dislocation or late instability are fortunately not common sequela of capsular injuries; however, when they do occur, surgery is required.

Finger Injuries↗

Stacking in capillary zone electrophoresis.

Due to the short light path of the capillaries, the CE detection limit based on concentration, is far less than that of HPLC and not sufficient for many practical applications. Several methods, based on different electrophoretic maneuvers, can concentrate the sample (stack) easily on the capillary before the separation step of capillary zone electrophoresis (CZE). These methods incorporate different types of discontinuous buffers as the means for invoking different velocities to the same analyte molecules to produce a sharpening of the band (stacking). In CZE, these buffers can be often very simple such as sample dilution or adding to the sample a high concentration of a fast mobility ion. However, in other applications these buffers can be as complicated as those required for isotachophoresis. Stacking can often yield a concentration factor of 5-30-fold, which can improve greatly in CZE the detection limits bringing them very close to those of HPLC. Different methods of stacking, the importance of discontinuous buffers and the different mechanism for concentration on the capillary are reviewed here. As there is a need for more practical applications, there will be more methods devised for stacking in CZE.

Acetonitriles↗

Comparison of ethylammonium formate to methanol as a mobile-phase modifier for reversed-phase liquid chromatography.

Ethylammonium formate (EAF), (C2H5NH3+HCO2-), is a room-temperature ionic liquid that has a polarity similar to that of methanol (MeOH) or acetonitrile. The separation at 1 mL/min of a test mixture of vitamins or phenols on a polystyrene-divinylbenzene column using either an EAF- or MeOH-water mobile phase is similar in terms of both resolution and analysis time. Because the viscosity of EAF is higher than that of MeOH, the plate count for phenol at room temperature is lower by about a factor of 1.1-1.4 depending on the flow rate. However, van Deemter plots show that this loss in plate count at 1 mL/min can be recovered and improved from 1500 to 2400 plates by working at a slightly elevated temperature of 55 degrees C. A slower flow rate such as 0.8 mL/min can also substantially improve the plate count as compared to 1-1.5 mL/min. Log P (octanol partition coefficient) versus log k' data for a variety of neutral test solutes are again similar whether EAF or MeOH is used as the organic modifier. Resolution of certain peak pairs such as 2,4-dinitrophenol/2,4,6-trinitrophenol and p-aminobenzoate/benzoate is enhanced using EAF as compared to MeOH. One advantage of EAF is that control of retention of solutes such as water-soluble vitamins under totally aqueous mobile phase conditions is environmentally preferable for quality control applications. In addition, EAF seems to be a milder mobile-phase modifier than MeOH for certain proteins such as lysozyme.

Journal Article↗

Fluorescence recovery after photobleaching: application to nuclear proteins.

Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological research. The method was developed in the 1970s, when its biological application mainly focused on the mobility of fluorescently labelled constituents of the cell membrane. The development of confocal scanning microscopy in the 1980s facilitated accurate investigation of the behaviour of molecules in the inside of cells without specialised equipment. However, FRAP did not yet become as popular as it is today, probably because of the dedicated and time-consuming methodology required to purify and label proteins or other compounds and, moreover, to inject them into cells. The revolution created by the development of GFP-technology finally lead to a tremendous boost of FRAP applications in studying the behaviour of proteins in the living cells. Finally, the ongoing increase of speed and memory of personal computers allows computer modelling of FRAP experiments for analysis of complex 3-D FRAP data, and for the development of new FRAP assays. Here we discuss several variants of FRAP on the basis of its application to the investigation of the behaviour of proteins in the living cell nucleus.

Active Transport, Cell Nucleus↗

[Prostaglandin E2--an effective alternative for the induction of labor].

The authors aimed at studying the local application of PgE2 as a method of labor induction. 50 pregnant women divided into 3 groups were studied: I with Prostin E2 - vaginal tablets of 3 mg. Dinoprostone. II with Prepidil gel - 0.5 mg. Dinfprostone, applied intracervically and III with additional stimulation with Oxytocin - 5 E as an i.v. infusion. The criteria used were: parity, gestational age, Bishop score indications for induction. Success is considered as normal delivery by the 24th hour. Indications for PgE2 application are prematurity praeeclampsia, fetal malformations, fetal death, grave obstetrics history, RH incompatibility. It was discovered that with Prostin E2, the active phase of labor is reduced thus leading to reduction of labor. The frequency of operative deliveries also reduced and there were better fetal outcome. Two important advantages were discovered--simple application and physiologic advantages--the woman in labor is mobile and thus not traumatised. The authors stress that PgE2 application can be considered an effective method for labor induction.

Administration, Intravaginal↗

Effects of dinucleoside polyphosphates on trabecular meshwork cells and aqueous humor outflow facility.

The most important risk factor for the development of glaucoma is elevated intraocular pressure (IOP). Hypotensive drugs decrease IOP, preventing optic nerve damage and further vision loss. The balance between aqueous humor (AH) production and drainage determines IOP, and problems in AH outflow pathways are associated with open-angle glaucoma development. Previous studies have shown the presence of diadenosine tetraphosphate (Ap(4)A) and pentaphosphate (Ap(5)A) in the AH. Topic application of Ap(4)A to the cornea decreased IOP, whereas Ap(5)A increased it. Because dinucleoside polyphosphates stimulate P2Y purinergic receptors, we studied their presence in trabecular meshwork (TM) cells. Additionally, the effects of diadenosine polyphosphates (Ap(n)As; n = 3-5) and Up(4)U (P(1),P(4)-(diuridine 5')-tetraphosphate; INS365) in outflow facility were tested. P2Y(1), P2Y(2), and P2Y(4) receptors were detected in TM cells by Western blot and immunocytochemistry. In TM cells, Ap(3)A, Ap(4)A, and Ap(5)A induced discrete intracellular calcium concentration ([Ca(2+)](i)) mobilizations compared with higher and more sustained [Ca(2+)](i) mobilizations after Up(4)U application. In bovine ocular anterior segments perfused at constant pressure, 1 microM Ap(3)A or Ap(4)A increased outflow facility, whereas Up(4)U or Ap(5)A did not modify it. 2-MeSADP, a selective P2Y(1) agonist, induced outflow facility increases similar to those obtained after Ap(3)A and Ap(4)A, and these were prevented by addition of the selective P2Y(1) receptor antagonist MRS-2179 (2'-deoxy-N(6)-methyladenosine-3',5'-diphosphate). Our results demonstrate that the hypotensive effect of Ap(4)A and other dinucleotides is mediated, at least in part, by increasing trabecular outflow facility through activation of P2Y(1) receptors. The latter would seem to be an interesting target in the development of antiglaucomatous drugs to selectively increase AH outflow.

Animals↗

Wearable systems for health care applications.

OBJECTIVES: Wearable systems can be broadly defined as mobile electronic devices that can be unobtrusively embedded in the user's outfit as part of the clothing or an accessory. In particular, unlike conventional mobile systems, they can be operational and accessed without or with very little hindrance to user activity. To this end they are able to model and recognize user activity, state, and the surrounding situation: a property, referred to as context sensitivity. Wearable systems range from micro sensors seamlessly integrated in textiles through consumer electronics embedded in fashionable clothes and computerized watches to belt worn PCs with a head mounted display. The wearable computing concept is part of a broader framework of ubiquitous computing that aims at invisibly enhancing our environment with smart electronic devices. The goal of the paper is to provide a broad overview of wearable technology and its implications for health related applications. METHODS: We begin by summarizing the vision behind wearable computing. We then describe a framework for wearable computing architecture and the main technological aspects. Finally we show how specific properties of wearable systems can be used in different health related application domains. RESULTS: Wearable computing is an emerging concept building upon the success of today's mobile computing and communication devices. Due to rapid technological progress it is currently making a transition from a pure research stage to practical applications. Many of those applications are in health related domains, in particular, health monitoring, mobile treatment and nursing. CONCLUSIONS: Within the next couple of years wearable systems and more general ubiquitous computing will introduce profound changes and new application types to health related systems. In particular they will prove useful in improving the quality and reducing the cost of caring for the aging population.

Clothing↗

Increase of intracellular calcium induced by oxytocin in hypothalamic cultured astrocytes.

A recent study demonstrated oxytocin (OT) receptors on hypothalamic cultured astrocytes (Di Scala-Guenot and Strosser, 1992). The attempt in the present paper was to determine a possible intracellular calcium mobilization induced by OT receptor activation in these cells. Using the microspectrofluorimetric technique with fura-2, as calcium indicator, brief applications of OT on single astrocytes induced a transient and reversible dose-dependent increase of intracellular calcium concentration ([Ca2+]i) in most of the cells tested. In a few cells, OT application triggered intracellular calcium oscillations. Repetitive applications of OT generally produced a decreasing calcium signal, suggesting a desensitization of the receptor. OT-induced calcium release was prevented by a prior or simultaneous application of an OT antagonist. The origin of the calcium mobilization was assessed during conditions where no extracellular calcium was available. Neither removal of extracellular calcium nor addition of a calcium channel blocker, cadmium 100 microM, in the bathing solution, did affect the calcium response to OT, demonstrating that release of intracellular calcium is solely involved in the OT-induced [Ca2+]i increase. The OT-induced calcium mobilization was abolished after thapsigargin application (100 nM). This indicates that the calcium response to OT application was principally associated with activation of the IP3-sensitive calcium stores. Taken together these results demonstrate that OT receptors previously detected on hypothalamic cultured astrocytes are functional receptors which transduction pathways involve calcium mobilization from IP3-sensitive stores.

Animals↗

Study of the suitability of HNO3 and HCl as extracting agents of mercury species in soils from cinnabar mines.

The aim of this study was to compare the influence and feasibility of two common extracting agents (50% v/v HCl and 50% v/v HNO3) on the leaching of Hg from soils. The solubility of a number of Hg species in each acid solution was evaluated under selected conditions. Most species were quantitatively dissolved in both acids with the exception of HgS. The application of both acid solutions to a soil sample from the Almaden mining area provided different recoveries of Hg: about 5% in 50% v/v HNO3 and 50% in 50% v/v HCl. The following experiments were designed and developed in order to evaluate the matrix influence on HgS solubility and leaching: (1) study of the solubility of HgS in the presence of different potential interfering compounds such as FeCl3, KCl, KI, Fe2O3, CuSO4, FeSO4, MnO2 and NaNO3; (2) study of the recovery of HgS spiked in soil samples; (3) study of the extraction process in soil samples spiked with the critical interfering compounds. Results showed the existence of a greater matrix influence with the HCl solution, since much higher Hg recoveries were obtained with this reagent. In addition, the presence of nitrates and Mn oxides drastically promotes the solubility of HgS in an HCl solution. On the other hand, halide compounds drastically enhanced the extractability of Hg in the HNO3 and they must be considered as potential interfering compounds when this acid solution is used as extracting agent. In summary, neither acid is totally free of matrix effects from common soil constituents; conclusions about mercury mobility resulting from the general application of these extraction procedures must therefore be made with caution.

Journal Article↗

Mobile access to biological databases on the Internet.

We have developed a new way of accessing biological databases and bioinformatics applications on the Internet. This new service, bioinformatics wireless application protocol (BioWAP) service, which is accessible by mobile devices makes it possible to access bioinformatics services, where normal PC or personal digital assistant (PDA) connections are not feasible. The BioWAP service includes major biological databases and applications demonstrating a simple method of implementing WAP interfaces to uncompliant applications, i.e. the applications that are not WAP or Internet based. The BioWAP service can be browsed with any WAP terminal.

Computational Biology↗

Glycogen is mobilized during the disposal of peroxides by cultured astroglial cells from rat brain.

Regeneration of reduced nicotinamide adenine dinucleotide phosphate (NADPH) is essential for the activity of glutathione redox cycling during cellular peroxide detoxification. In order to test for a function of astroglial glycogen to serve as endogenous precursor for glucose-6-phosphate, the substrate for the regeneration of NADPH by the pentose phosphate pathway, the content of glycogen in astroglia-rich primary cultures derived from the brains of newborn rats was determined after application of peroxides. In the presence of hydrogen peroxide or cumene hydroperoxide in concentrations of 200 microM glycogen was mobilized with a half-life of 16 min in incubation medium containing 20 mM glucose, whereas in the absence of peroxides the glycogen content decreased more slowly with a half-life of 42 min. After 30 min of incubation with or without peroxides 30 and 73%, respectively, of the initial glycogen content was found. The degree of glycogen mobilization was reduced by lowering the initial concentration of the peroxides. These results demonstrate that in astroglial cells (i) glucosyl residues of glycogen are mobilized after application of peroxides despite the presence of exogenous glucose, and (ii) that the demand for glucose-6-phosphate as substrate for NADPH regeneration via the pentose phosphate pathway can, at least partially, be met by mobilization of glycogen.

Animals↗

Recent innovation in capillary electrokinetic chromatography with replaceable charged pseudostationary phases or additives.

Recent developments of separation of neutral analytes in capillary systems with the mobile phase driven by the electroosmotic flow (EOF) and charged additives acting as a pseudostationary phase are reviewed. As pseudostationary phases a number of additives are used. Soluble polymers, either anionic or cationic, were applied as alternatives to micelles. Monomeric charged additives are also intended to form associates with the analytes, leading to selective retention and separation in a similar way as the polymeric pseudostationary phases. Dendrimers, spherical macromolecules with highly branched chains and charged terminal groups, are successfully applied for the separation of lipophilic analytes. Polymers with covalently stabilized structures are introduced in the form of permanent micelles and are therefore insensitive to the mobile phase composition, enlarging the applicability of micellar electrokinetic capillary chromatography (MEKC).

Chemistry, Organic↗

A functional analysis of the P-element gene-transfer vector in insects.

A P-element mobility excision assay was used to determine if non-drosophilid insects could support P gene vector function. Present studies included the testing of Muscids, Sphaerocerids, and Phorids, none of which were able to support P mobility. A new excision indicator plasmid was developed allowing the detection and recovery of virtually all P-element excision products. The frequency and sequence analysis of excision products from Drosophila melanogaster and another drosophilid, Chymomyza procnemis, indicated both quantitative and qualitative differences in the activity of transposase. The quantitative relationships observed in the original assay were maintained, and qualitative differences in transposase activity were reflected in the sequence of the empty donor sites. The results suggest that host factors are involved in cutting and ligating P-element DNA during excision, with transposase facilitating these processes. Possible limitations on P mobility by abnormal transposase transcript processing were tested in Anastrepha suspensa using transposase-encoding plasmids having deleted intron sequences. A transposase cDNA supported normal P excision in D. melanogaster, and a low level of mobility in A. suspensa. Possible applications of gene transfer in insects are presented, in particular methods to genetically sterilize and sex insects for the sterile-insect technique.

Animals↗

In pursuit of the whole hypha.

We know organisms first of all by their forms. Rabbit and carrot, Neurospora, and Paramecium represent particular shapes and structures, patterns in space and time. Each pattern integrates innumerable molecules into a coherent whole, reproduces itself from one generation to the next, and may persist in this manner for millions of years. In this lecture, I shall discuss efforts to render a dynamic and causal account of biological morphogenesis, using fungal hyphae as a concrete exemplar. Molecular structures and interactions are necessary but not sufficient to specify patterns on a scale three to five orders of magnitude larger. The search for alternatives leads to the importation of the concept of dynamic fields, exemplified by the proposal of Bartnicki-Garcia and Gierz that apical growth and morphogenesis report the operation of a mobile vesicle-supply center. Application of field theories to biological morphogenesis is still at an early stage, but is necessary in order to resolve the paradoxical relationship between genes and form.

Cell Wall↗

High-performance gel permeation chromatography of proteins and peptides on columns of TSK-G2000-SW and TSK-G3000-SW: a volatile solvent giving separation based on charge and size of polypeptides.

Trifluoroacetic acid (0.1% w/v) is an excellent solvent for polypeptides, is volatile, and has a low absorbance of ultraviolet light of low wavelength. Polypeptides subjected to chromatography on columns of TSK-G2000-SW or TSK-G3000-SW in this solvent were eluted as sharp peaks. Retention volume was dependent not only on molecular weight but also on the number of formal charges per molecule. For polypeptides with a similar molecular weight, that with the highest proportion of basic amino acid residues was eluted earliest. For the TSK-G2000-SW column, the degree of deviation from a linear relationship between elution volume and logarithm of molecular weight was directly proportional to the molecular weight to the power 2/3 divided by the number of positive charges per molecule. Inclusion of 0.25 M sodium chloride in the solvent increased both the upper and lower limits of the molecular weight range over which separation occurred. The use of 0.1% trifluoroacetic acid as the mobile phase is thus particularly applicable to the separation of peptides of low molecular weight.

Chromatography, Gel↗

P transposition in Drosophila provides a new tool for analyzing postreplication repair and double-strand break repair.

A genetic screen has been developed in Drosophila for identifying host-repair genes responsible for processing DNA lesions formed during mobilization of P transposable elements. Application of that approach to repair deficient mutants has revealed that the mei-41 and mus302 genes are necessary for recovery of P-bearing chromosomes undergoing transposition. Both of these genes are required for normal postreplication repair. Mutants deficient in excision repair, on the other hand, have no detected effect on the repair of transposition-induced lesions. These observations suggest that P element-induced lesions are repaired by a postreplication pathway of DNA repair. The data further support recent studies implicating double-strand DNA breaks as intermediates in P transposition, because the mei-41 gene has been genetically and cytologically associated with the repair of interrupted chromosomes. Analysis of this system has also revealed a striking stimulation of site-specific gene conversion and recombination by P transposition. This result strongly suggests that postreplication repair in this model eukaryote operates through a conversion/recombination mechanism. Our results also support a recently developed model for a conversion-like mechanism of P transposition (Engels et al., 1990). Involvement of the mei-41 and mus302 genes in the repair of P element-induced double-strand breaks and postreplication repair points to a commonality in the mechanisms of these processes.

Animals↗

The effect of walking speed on lower-extremity joint powers among elderly adults who exhibit low physical performance.

OBJECTIVES: To compare peak joint powers and joint angles between comfortable and fast walking speeds among a group of elderly adults who exhibit low physical performance, and to test the primary hypothesis that peak ankle powers would not change when walking speed was increased, but that peak hip power output would increase significantly with speed. DESIGN: Three-dimensional analysis of joint kinematics and kinetics during comfortable and fast walking by both healthy and low-performing elderly adults (age, >70y). SETTING: Gait laboratory. PARTICIPANTS: Twenty-four healthy elderly adults and 27 elders who exhibited low performance on a standard battery of walking, standing balance, and chair-rise tasks that places them at risk of mobility-related disability. INTERVENTIONS: Not applicable. MAIN OUTCOME MEASURES: Peak lower-extremity joint powers and joint angles. RESULTS: Low-performing elders increased both ankle and hip power outputs to increase walking speed. However, peak ankle power remained significantly below that of the healthy elderly adults even when the low-performing elders walked at a faster gait speed. Joint-power changes in the low-performing elderly were accompanied by a reduction in hip extension and ankle dorsiflexion, and an increase in transverse pelvic rotation. CONCLUSIONS: Compared with healthy elderly, the low-performing elderly adults showed speed-independent differences in ankle and hip mechanics that may reflect underlying neuromuscular impairments. In particular, an understanding of the interdependent contributions of hip flexibility and ankle power limitations seem important to inform interventions to maintain gait into advanced age.

Aged↗

GEA3162, a nitric oxide-releasing agent, activates non-store-operated Ca2+ entry and inhibits store-operated Ca2+ entry pathways in neutrophils through thiol oxidation.

We demonstrated that 5-amino-3-(3,4-dichlorophenyl)1,2,3,4-oxatriazolium (GEA3162), a nitric oxide (NO)-releasing agent, stimulated [Ca2+]i rise in rat neutrophils. This Ca2+ response was prevented by the thiol reducing agents, 2-mercaptoethanol, N-acetyl-L-cysteine, dithiothreitol, 2,3-dimercaptopropane-1-sulfonic acid (DMPS) and tris-(2-carboxyethyl)phosphine (TCEP), but slightly reduced by the antioxidant, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). GEA3162 also increased the formation of cellular reactive oxygen intermediates and decreased the cellular content of low molecular thiols. These responses were greatly reduced by Trolox, dithiothreitol and N-acetyl-L-cysteine. GEA3162 stimulated the protein tyrosine phosphorylation in neutrophils. The [Ca2+]i rise caused by formyl-Met-Leu-Phe (fMLP) and cyclopiazonic acid (CPA) was suppressed by GEA3162. TCEP prevented the inhibition of fMLP-induced [Ca2+]i rise by GEA3162. In the absence of external Ca2+, GEA3162 inhibited the CPA-induced [Ca2+]i rise, whereas it only slightly affected the fMLP-induced mobilization of the Ca2+ store. Application of GEA3162 after the stimulation with fMLP or CPA suppressed the robust Ca2+ entry followed by the readdition of Ca2+ into medium. Moreover, the Ca2+ entry was more susceptible to inhibition by treatment with GEA3162 prior to than after the fMLP stimulation. GEA3162 had no effect on the mitochondrial membrane potential. GEA3162 induced actin reorganization and condensed filament network at the cell periphery. These results indicate that GEA3162 exerted both the stimulation of Ca2+ entry and the inhibition of the store-operated Ca2+ entry in rat neutrophils. The dual effects of GEA3162 on the regulation of the external Ca2+ entry are mainly through the thiol modification of target protein(s) residing on the outside of the plasma membrane.

Acetylcysteine↗