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PubMed1964

ASSOCIATION OF RAPIDLY METABOLIZED DNA AND RNA.

An association of rapidly metabolized DNA and RNA labeled with P(32) has been found in peanut cotyledons by incorporation of either uridine-C(14) or thymidine-C(14) by peanut cotyledon slices. Chromatography of the extracted nucleic acids on methylated- albumin columns shows that the rapidly metabolized DNA and RNA are contained only in the first half of the DNA peak where RNA and DNA occur in equal amount. One-fourth of the radioactivity is recovered in ribonuc'eotides. Density-gradient centrifugation in cesium chloride indicates that the material containing the P(32) has a higher density than most of the DNA.

Carbon Isotopes↗
PubMed1965

COUPLING OF PHOSPHORYLATION AND CARBON DIOXIDE FIXATION IN EXTRACTS OF THIOBACILLUS THIOPARUS.

Johnson, Emmett J. (University of Mississippi Medical Center, Jackson), and Harry D. Peck, Jr. Coupling of phosphorylation and carbon dioxide fixation in extracts of Thiobacillus thioparus. J. Bacteriol. 89:1041-1050. 1965.-A cell-free system from Thiobacillus thioparus which fixes large quantities of C(14)O(2) in the presence of ribose-5-phosphate, adenosine triphosphate (ATP), and Mg(++) has been described. The specific activity (0.041 mumole of ribulose-1,5-diphosphate min(-1) mg(-1) protein) of the CO(2)-fixing system approaches that of green plants, and is further evidence for the importance of the role of carboxydismutase in the thiobacilli. In addition to ATP, adenosine diphosphate (ADP) and other nucleoside triphosphates served with varying degrees of effectiveness for the fixation of C(14)O(2). The ATP requirement for CO(2) fixation was partially replaced under aerobic conditions by a combination of SO(3) (=), PO(4) ( identical with), and adenosine monophosphate (AMP). Phosphorylation and CO(2) fixation were separated in time by first incubating SO(3) (=) and AMP aerobically, and then anaerobically introducing C(14)O(3) (=) and ribose-5-phosphate into the reaction mixture. During the first incubation, P(32)O(4) ( identical with) was esterified into nucleotides, mainly ADP, and in the second incubation C(14)O(2) was fixed, with the concomitant utilization of almost equal amounts of the esterified phosphate. These data provide the first in vitro evidence for the mechanism of the coupling of CO(2) fixation and phosphorylation in T. thioparus. The fixation of C(14)O(2) was shown to be almost completely inhibited by AMP. This inhibition was not due to the conversion of ATP to ADP by adenylic kinase, or to the binding of magnesium by the nucleotide. The inhibition was specific for AMP, since other mononucleotides, adenosine, and adenine did not inhibit. The AMP regulation of CO(2) fixation may represent a basic control mechanism in autotrophic metabolism.

Adenine Nucleotides↗