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PubMed2006

1-Iodo-2methylundecane, a putative estrus-specific urinary chemo-signal of female mouse (Mus musculus).

The present study was designed to identify the urinary volatiles across various reproductive stages of female mice in order to detect estrus-specific chemical signal. The urine of adult female mice were extracted with dichloromethane (1:1 ratio, v/v) and analyzed by gas chromatography linked mass-spectrometry (GC-MS). Numerous compounds were identified during estrus cycle of female mice urine. Among these, the compounds, namely, isocroctylhydrazine, 4-methyl-2-heptanone and auzulene were specific to proestrus stage and the compounds, 1-H-cyclopop-e.auzulene, caryophyllene, copanene were specific to estrus stage. However, the compound, 1-iodo-2methyl undecane (1I2MU) was observed both in proestrus and estrus phases and was absent in all other phases. The volatile signal produced at the end of proestrus and the beginning of estrus phase appears to be behaviourally important in the attraction of males. Moreover, the behaviour assay revealed that the compound, 1I2MU, is involved in attracting the male mice. This result concludes that the 1-iodo-2methyl undecane is considered as a putative estrus-specific chemo-signal.

Animals↗
PubMed2002

Lactational estrus induction in the Mexican hairless sow.

The purpose of this study was to determine the effects on the reproductive performance of the Mexican hairless pig (MHP) while inducing fertile lactational estrus. Lactational estrus was induced on day 8, postpartum, in 20 primiparous sows during two consecutive reproductive cycles. Boar presence and temporary litter withdrawal were used as stimuli. Group I did not receive stimuli (control, C); group II had a 15 min boar stimulus (B); group III had litter withdrawal for 4 h (LW), and group IV received both stimuli (B + LW). Fertile estrus was detected by sow behavior, exfoliative vaginal cytology, and 17 beta-estradiol and progesterone samples. Individual stimuli did not induce fertile lactational estrus; nevertheless, when used together, both stimuli induced 100% of females into estrus, and 80% of them got pregnant while lactating. The partial withdrawal of the litter had a significant and positive effect on the number of live born piglets. Sows pregnant during lactation had shorter reproductive cycles, by not having non-productive days and litter size was greater. Pregnancy induction during lactation allowed the Mexican hairless sow to reduce the period between parturition's without diminishing the lactation length and litter performance.

Animals↗
PubMed1998

Induced and synchronized estrus in cattle: dose titration of estradiol benzoate in peripubertal heifers and postpartum cows after treatment with an intravaginal progesterone-releasing insert and prostaglandin F2alpha.

Peripubertal beef heifers (n = 57) and postpartum multiparous cows (n = 52) were used to determine the optimal dose of estradiol benzoate (EB) to induce and synchronize estrus after treatment with intravaginal progesterone inserts (IVP4, EAZI-BREED CIDR). All females received an IVP4 for 7 d (d 0 = insertion day) with a 25-mg injection of PGF2alpha (Lutalyse) on d 6. At 24 to 30 h after IVP4 removal, females were randomly assigned to be injected subcutaneously with EB at the following doses: heifers 0, .2, .38, or .75 mg and cows 0, .25, .5, or 1 mg. Furthermore, seven heifers and seven cows from each dose group were bled every 4 h for 76 h starting at EB injection. Serum was collected and assayed for LH and estradiol-17beta (E2). Observations for signs of estrus were made twice daily for 21 d after removal of IVP4, and females were artificially inseminated 8 to 20 h after detection of estrus. The percentage of females showing estrous behavior was increased by EB (P < .04); the greatest response was at .38 mg in heifers (86%) and 1 mg in cows (100%). Dose x time interaction affected (P < .01) E2 concentrations in heifers and cows; the animals that received the higher doses of EB had greater E2 concentrations in a shorter time than those that received the smaller doses. The percentage of cows and heifers with an acute preovulatory LH release (peak LH) was affected by dose, with a linear (P < .01) and a quadratic (P < .01) response. Highest concentrations of LH during peak LH were affected by dose with a linear (P < .01) response in heifers and linear (P < .01) and quadratic (P < .08) responses in cows. Heifers receiving .38 mg and cows receiving .5 and 1 mg of EB had the highest peak LH. Time to LH peak had a linear (P < .03) response in heifers and had linear (P < .04) and quadratic (P < .05) responses in cows. Pregnancy rate was affected (P < .02) in heifers by whether or not they were anestrous before IVP4 treatment (those with estrous cycles = 52% vs those that were anestrous = 22%) and in cows by dose of EB (P < .01; 8, 23, 21, and 67% for 0, .25, .5, and 1 mg, respectively). In conclusion, in females treated with IVP4 and PGF2alpha to induce and synchronize estrus, an injection of EB increased concentrations of E2 and LH and increased number of animals showing estrus. Also, EB increased pregnancy rates in cows. Optimal responses were at .38 mg EB for heifers and at 1 mg EB for cows.

Administration, Intravaginal↗
PubMed1984

Inability of progestogen pretreatment to prevent premature luteolysis of induced corpora lutea in the anestrous bitch.

Fourteen mature anestrous bitches were used to determine the effectiveness of pretreatment with an orally active progestogen to prevent premature luteolysis of induced corpora lutea (CL) in the anestrous bitch. In Group 1, seven bitches were treated orally with megestrol acetate (Ovaban((R))) at the rate of 2.2 mg/kg body weight for eight days. Three days later, the bitches were treated daily with pregnant mare's serum gonadotropin (PMSG) (44 IU/kg body weight) administered intramuscularly for nine consecutive days, and each bitch was given 500 IU human chorionic gonadotropin (HCG) on day 10, or on the first day of induced estrus if the bitches exhibited estrus while being treated with PMSG. A control group (Group 2) of seven bitches was not treated with Ovaban((R)) but was similarly given PMSG and HCG. Estrus was detected twice daily using a vasectomized male dog and verified by vaginal cytology. Blood samples were obtained on the first day of induced estrus (day 0) and every other day until day 90 post-estrus. Plasma progesterone (P(4)) concentrations were determined by a non-extraction solid phase radioimmunoassay (RIA), and data were analyzed by Student's t-test. There was no significant difference between the progesterone profiles of both groups of bitches. In addition, P(4) values were less than 1 ng/ml by day 50 post-estrus. Results of this study suggested that pretreatment with an orally active progestogen was not effective in preventing premature luteolysis of induced CL in the anestrous bitch.

Journal Article↗
PubMed1993

Effects of diets containing gossypol on ovarian histology, function and fertility in prepubertal beef heifers.

Forty-five crossbred beef heifers (weight = 268.3 +/- 5.7 kg) were used to determine the effects of dietary gossypol on ovarian morphology, erythrocyte fragility and fertility. Heifers were randomly assigned to 1 of 3 isonitrogenous dietary treatments. The diet consisted of rice mill feed and milo supplemented with soybean meal (n = 13; control), cottonseed meal (n = 16; low supplementation) which supplied 6.1 g free gossypol animal(-1) day(-1), or whole cottonseed (n = 16; high supplementation) which supplied 13.7 g free gossypol animal(-1) day(-1). The heifers were group-fed each diet for 64 days and were maintained on similar but separate fescue pastures overseeded with wheat. After 64 days, 4 heifers from each diet were confined and fed their respective diets. On Day 10 following estrus, each animal was unilaterally ovariectomized, and the ovary containing the corpus luteum was removed. The remaining ovary was removed 6 to 12 hours after detection of estrus in the next cycle. Erythrocyte fragility increased (P < 0.02) in heifers receiving gossypol compared with that of the controls. Cyclicity in the heifers was 81.3, 68.8 and 38.4% for high, low and control diets, respectively, at the end of the 64-day treatment period. First service conception rate, as determined by palpation per rectum, was similar among treatments (58.3, 33.3, 33.3% for high, low and control groups, respectively). Weight gain increased (P < 0.03) in control heifers compared with that of heifers receiving gossypol. Gross ovarian morphology and histology were similar for all heifers. Although gossypol produced mild toxicosis in heifers, no adverse reproductive effects could be detected from gossypol intake.

Journal Article↗
PubMed1988

Effect of estrous cycle stage at Syncro-Mate B treatment on conception and time to estrus in cattle.

Two trials involving 85 heifers and 67 cows were conducted to determine the effect of estrous cycle stage at the time of Syncro-Mate-B((R)) (SMB) treatment on interval to estrus following implant removal and on conception rate at the synchronized estrus. In Trial 1, 57 beef and 28 dairy heifers were treated with SMB on each representative day of a 22-d estrous cycle (estrus = Day 0). Beef heifers were artificially inseminated approximately 48 h after implant removal, whereas dairy heifers were inseminated 0 to 12 h after detection of estrus. Inseminations were scored by the inseminator according to their difficulty. Interval to the onset of estrus was not different between heifers treated early (</= Day 11) in the estrous cycle (31.3 +/- 7.4 h) and heifers treated late (> Day 11) in the cycle (35.2 +/- 7.2 h). Conception rate at the synchronized estrus was slightly higher in early-cycle heifers (22 47 = 47% ) compared to late-cycle heifers (14 38 = 37% , P = 0.2). Heifers that were difficult to inseminate had lower (P < 0.01) conception rates (2 11 = 18% ) at the synchronized estrus than heifers considered normal (21 51 = 41% ) or easier than normal to inseminate (13 23 = 57% ). In Trial 2, of the 131 beef cows synchronized, 67 that were estimated to be either early or late in the estrous cycle by progesterone analysis were utilized. Cows were treated with SMB and inseminated without regard to estrus 48-h after implant removal. Inseminations were scored as in Trial 1. Calves were separated from cows from the time of implant removal to insemination. Conception rate was higher (P < 0.05) in cows treated with SMB early (</= Day 11) in the estrous cycle (20 32 = 62.5% ) compared to cows treated late (> Day 11, 16 35 = 46% ). Cows that were difficult to inseminate had a lower (P < 0.01) conception rate (0 8 = 0% ) than cows that were normal (43 94 = 46% ) or easier than normal to inseminate (13 29 = 45% ).

Journal Article↗
PubMedDate not supplied

Oestrus in the British white goat: timing of plasma luteinizing hormone surge and changes in behavioural and vaginal traits in relationship to onset of oestrus.

Length of oestrus and timing of the LH surge was measured in six British White does, housed with a vasectomized buck (experiment 1). The following breeding season, pulsatile LH release during the cycle was measured in eight does and the pattern of behavioural and vaginal traits in relation to onset of oestrus (time 0) determined (experiment 2). In experiment 1, the interval to first oestrus after introduction of the buck on 1st October was 10.3 +/- 3.0 days (n = 6) but in experiment 2, when the buck was put in on 1st September, first oestrus occurred after 39.3 +/- 3.4 days in 7/8 does and 7 days in 1/8 does indicating that adequate exposure to short days is needed before the buck can initiate ovarian activity. LH pulse frequency increased from 0-1 pulses/8 h to 3 pulses/8 h after luteolysis, with no change in pulse amplitude, suggesting that progesterone regulates LH pulse frequency. Mean LH values rose from basal to 102.1 +/- 7.8 ng/ml, 12 +/- 1.5 h after the onset of oestrus, which was 16.3 +/- 1.7 h in length. Does sought out the buck and displayed tail wagging, bleating and restlessness from -60 h to +36 h relative to the onset of oestrus (time 0). The incidence of these activities rose at -12 h and peaked at 0 h. Tail wagging, but not bleating or restlessness, also increased in intensity at 0 h, as did the intensity with which the doe actively sought out the buck. Vulval redness and swelling and onset of a clear thin vaginal discharge were first observed 1-2 days before oestrus, becoming maximal on day 0. It was concluded that onset of frequent tail wagging was the most useful trait for detecting onset of oestrus.

Animals↗
PubMed1983

Evaluation of tail paste as an oestrus detection aid using serial progesterone analysis.

Serial progesterone assay of 127 commercial dairy cows over several months identified 363 possible oestrous events. Of these, tail paste correctly identified 81.5 per cent, while the stockmen unaided recorded only 70.2 per cent (national average 55 to 60 per cent). The significance of such a degree of improvement, the factors restricting oestrus detection efficiency and the difficulties in creating the appropriate mental attitude to oestrus detection improvements are discussed.

Animals↗
PubMed1991

Oestrous cycle of the North American bison (Bison bison) characterized by urinary pregnanediol-3-glucuronide.

An enzyme immunoassay for urinary pregnanediol-3 alpha-glucuronide (PdG) was evaluated for the indirect measurement of progesterone metabolites during the oestrous cycle and early pregnancy of uncaptured North American bison. Comparisons between plasma progesterone and urinary PdG, dose-response parallelism between the standard curve and diluted urine samples and high-performance liquid cochromatography revealed that PdG was a primary immunoreactive urinary metabolite of progesterone in bison. Urine samples were collected directly from the soil from 29 bison cows during the August rutting season and analysed for PdG. Eight bison cows demonstrated complete oestrous cycles ranging from 19 to 26 days (mean cycle length = 23.12 +/- 0.76 days) and behavioural oestrus among four of these cows correlated with PdG nadirs. Mean PdG nadirs were 63.62 +/- 21.61 ng/mg urinary creatinine (Cr) and mean peak midluteal values were 546.01 +/- 130.73 ng/mg Cr. Seven of eight became pregnant, indicating that bison exhibit a second seasonal oestrus. Eighteen other bison cows were pregnant prior to the beginning of the study and demonstrated non-cyclic increased PdG concentrations (greater than 200 ng/mg Cr) during the 30-day course of collection. Three cows ovulated and became pregnant during the 30-day collection period and then exhibited increasing urinary PdG concentrations. This report demonstrates that ovarian function in uncaptured bison can be monitored by means of urinary PdG and that both ovulatory cycles and early pregnancy can be detected.

Animals↗
PubMed1994

Protein intake and development of ovarian follicles and embryos of superovulated nonlactating dairy cows.

Nonlactating Holstein cows (n = 12) were assigned randomly to one of two diets (approximately 30% concentrate) in a crossover design. Urea and soybean meal were used to increase CP content of diet from 12.3 to 27.4%. Mean concentrations of plasma urea were 9.8 and 21.3 mg/dl for respective diets. After 35 d on diets, cows were brought to a synchronized estrus (progestin implant plus PGF2 alpha injection). From d 10.5 to 14.5 following estrus, cows received i.m. injections of decreasing doses of FSH to initiate superovulation. On d 3 of FSH treatment, PGF2 alpha was injected with FSH. Cows were bred twice by AI when detected in estrus. Embryos were collected nonsurgically 6.5 d after breeding, and quality was assessed using visual, microscopic, and staining techniques. Development of follicles throughout this period was monitored using ultrasonography. Using ultrasonography, no differences were detected in the number or percentages of preovulatory, anovulatory, and ovulatory follicles induced during superovulation. Number of recovered embryos averaged eight per cow. Numbers and percentages of normal embryos, abnormal or retarded embryos, and unfertilized ova were similar between diets, as were the numbers and percentages of transferable and nontransferable embryos. Excess intake of CP failed to affect the growth or number of ovarian follicles and health and number of embryos of energy adequate, nonlactating dairy cows.

Ammonia↗
PubMed1985

Mounting behavior as affected by stage of estrous cycle in Holstein heifers.

Nine Holstein heifers whose estrous cycles were synchronized were observed during three subsequent consecutive cycles. Observations of heifers during each periestrus period began when the first heifer reached day 15 of an estrous cycle and ended 36 h after onset of estrus for the ninth heifer. There were 118 1-h observations at 6-h intervals during the three periestrus periods. Estrous cycles were divided into postestrual, luteal, preestrual, and estrual stages by blood progesterone profiles and estrus. Heifers of these four stages accounted for 27.5, 48.3, 15.4, and 8.8% of heifers available to mount during the 118 observations, and heifers of each stage made 9.0, 5.2, 18.9, and 66.9% of attempted mounts. When number of heifers in estrus simultaneously increased from one to five, total attempted mounts and stands per hour increased from 10.1 to 63.5 and from 4.5 to 48.5. Stands per heifer per hour increased significantly as number of heifers in estrus increased from one to two and tended to increase as number in estrus increased further. Presence of one preestrual heifer in a group containing one estrual heifer increased total stands per hour from 2.3 to 5.0. Efficiency of detection of estrus may depend on proportion of animals within estrous cycle stages that have highest mounting activity.

Animals↗
PubMed1997

Ovulatory responses and plasma luteinizing hormone concentrations in dairy heifers after treatment with exogenous progesterone and estradiol benzoate.

The objectives of this experiment were to determine the effects of 0.5 mg estradiol benzoate, administered intramuscularly 24 h after removal of CIDR-B progesterone containing intravaginal devices, on the time to estrus, ovulation and peak LH concentration in dairy heifers. Ovulatory responses and plasma LH concentrations were examined using 14 Friesian dairy heifers in 2 separate treatment periods. All heifers received a CIDR-B progesterone-containing intravaginal device with an attached 10-mg estradiol benzoate capsule for 12 d. Within each period, 24 h after CIDR-B removal, 7 heifers received an intramuscular injection of 0.5 mg estradiol benzoate while the remaining 7 heifers received an intramuscular injection of a placebo. Blood samples for LH assay were collected at 0, 6 and 12 h, and then every 4 h for 60 h after estradiol injection. Detection of estrus was conducted at 4-h intervals, and ultrasonographical examination to detect ovulation was conducted every 8 h for 88 h after removal of the CIDR-B device. Treatment with estradiol benzoate tended to reduce the time from device removal to the LH peak in Period 1 (median time to LH peak 40.1 vs 63.9 h; P = 6.07). In Period 2, treatment with estradiol had no significant effect on the time to the LH peak, standing estrus or ovulation. We hypothesize that the period effect was due to the stage of cycle at the time of treatment. For heifers treated in Period 1, the stage of cycle was random. However, because of the prior synchronization of estrus, which was implicit in the experimental design, heifers in Period 2 tended to be in late diestrus. The administration of estradiol benzoate after treatment with exogenous progesterone appears to overcome the variability in timing of LH peaks typically occurring in a herd of synchronized heifers due to different stages of follicular development.

Journal Article↗
PubMed1996

Synchronization of estrus in yearling beef heifers with the melengestrol acetate/prostaglandin F(2alpha) system: efficiency of timed insemination 72 hours after prostaglandin treatment.

Two experiments were conducted to determine the conception rates of heifers time-inseminated following melengestrol acetate/prostaglandin F(2alpha) (MGA/PG) estrous synchronization treatment. In Experiment 1, timed insemination of heifers at 72 h after the PG injection, without regard for behavioral estrus, tended to improve (P < 0.15) the percentage of heifers pregnant to artificial insemination (AI) compared with that of synchronized heifers bred 12 h after they were first detected in estrus. In the timed-insemination treatment, heifers exhibiting behavioral estrus 48 to 72 h after PG tended to have an increased (P < 0.15) conception rate to AI compared with heifers exhibiting estrus within 48 h of PG administration. In Experiment 2, the number of heifers conceiving to AI following the MGA/PG estrous synchronization regimen was increased by mass insemination of all heifers not exhibiting estrus by 72 h after PG. The pregnancy rate to AI was higher in heifers with serum progesterone (P(4)) concentrations higher than 1 ng/ml compared with that of heifers with concentrations lower than 1 ng/ml. Of heifers with serum P(4) greater than 1 ng/ml, the pregnancy rate to AI tended to be higher when concentrations exceeded 2 ng/ml than when concentrations were 1 to 2 ng/ml. In cyclic heifers, timed insemination can increase the percentage of heifers pregnant after being synchronized with MGA/PG.

Journal Article↗
PubMed1995

[Continuous examination of sows with cystic, degenerating ovaries using sonography and serum hormone determination].

Ten sows with cystic ovaries have been examined periodically by transrectal sonography (2 days intervals) and analysis of serum-progesterone and estradiol (7 days intervals). Estrus occurred in nine of the sows after a time up to 82 days. Estrus reappeared after an almost physiological interval of 22 to 24 days. The cysts on the ovaries regressed completely (4 sows) or diminished in size (5 sows) before estrus. The regression was preceded by increasing echoes from trabeculae-like structures in the cysts and increase of serum progesterone concentration. In six sows cysts were again detectable after estrus. These increased in size and regressed again in the same manner as before. All nine sows were served by a boar during second estrus and seven became pregnant according to sonographic findings after 27 days. These results show that sows with cysts on there ovaries may maintain an ovarian cycle and even conceive. The regression of ovarian cysts is probably preceded by the formation of luteal tissue. Our results don't allow general conclusions concerning the role of ovarian cysts in sows as a cause of infertility due to the small number and the unknown anamnesis of our patients. However in only one of the ten sows, the cysts persistent beyond the observation time of 100 days.

Animals↗
PubMed2006

Effects of Zeranol upon luteal maintenance and fetal development in peripubertal gilts.

Eighty gilts were utilized to determine whether zeranol implants could maintain hCG-induced corpora lutea (CL) in peripubertal gilts and to examine the effects of a Zeranol implant on fetal development. Crossbred gilts (171+/-0.3 days of age, 109.1+1.4 kg) were blocked by weight and ancestry to control (n=40) or treatment (n=40) groups. To induce ovulation and CL maintenance, treated gilts received 500 IU of hCG i.m. and a Zeranol ear implant (Ralgro, 36 mg; day 0). All gilts were checked once daily for estrus with a mature boar from days 3-58 of the experiment. On day 42, treated gilts received two 10 mg injections of Lutalyse (PGF(2)alpha) spaced 6 h apart. Treated gilts not displaying estrus within 7 days of PGF(2)alpha received two additional 10 mg of PGF(2)alpha spaced 6 h apart on day 49. On days 44-58, gilts detected in estrus were inseminated twice, 24 h apart with pooled semen via AI. Blood samples were obtained on days 0, 7, 18 and 42 and analyzed for serum progesterone (P(4)). Bred gilts were slaughtered on days 58-62 of gestation. Ovulation, as determined by serum concentrations of P(4) on day 7 of the experiment, was induced by hCG in 79.5% of treated gilts. Zeranol implants, however, failed to increase (P>0.05) the proportion of gilts available for breeding (treated, 21/39; control, 18/40). Of gilts inseminated on days 44-58, 16/21 treated gilts and 16/18 control gilts were pregnant at slaughter on days 58-62 of gestation. Number of fetuses (7.5 versus 12), fetal weight (83 versus 121 g), fetal length (117 versus 132 mm) and fetal survival (45% versus 78%) were reduced (P<0.001) by Zeranol implants. These data indicate that treatment of peripubertal gilts with a 36 mg Zeranol implant did not increase the proportion of gilts available for breeding while causing deleterious effects upon the fetuses.

Animals↗