PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Sanger sequencing”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 469 records · Page 26Linked to original sources
PubMed2026

The genome sequence of a nematode, Thelazia callipaeda Railliet & Henry, 1910 (Rhabditida: Thelaziidae).

We present a genome assembly of Thelazia callipaeda (nematode; Nematoda; Chromadorea; Rhabditida; Thelaziidae). The genome sequence has a total length of 117.59 megabases. Most of the assembly (75.82%) is scaffolded into 4 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has been assembled, with a length of 13.66 kilobases.

Thelazia callipaeda; nematode; genome sequence; ch↗
PubMed1987

[Molecular cloning of genes coding for tumor necrosis factor. Complete nucleotide sequence of the genome copy of TNF-alpha in mice].

Using Sanger's technique and synthetic oligonucleotides derived from the known structure of cDNA, complete nucleotide sequence of the genomic copy of the mouse TNF-alpha gene has been determined. The length of the gene from transcription initiation site down to polyadenylation signal is about 2620 bp. Comparison with the previously described cDNA sequence revealed existence of 4 exons and 3 introns in the positions homologous to those of human TNF gene. The fourth exon codes for 88% amino acids of mature TNF-alpha.

Animals↗
PubMed2023

The genome sequence of a solitary sea squirt, Ascidia mentula (Müller, 1776).

We present a genome assembly from an individual Ascidia mentula (the (a solitary sea squirt); Chordata; Ascidiacea; Phlebobranchia; Ascidiidae). The genome sequence is 197.0 megabases in span. Most of the assembly is scaffolded into 9 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 19.46 kilobases in length.

(a solitary sea squirt)↗
PubMed2024

The genome sequence of the black-footed limpet, Patella depressa (Pennant, 1777).

We present a genome assembly from an individual Patella depressa (the black-footed limpet; Mollusca; Gastropoda; Patellogastropoda; Patellidae). The genome sequence is 683.7 megabases in span. Most of the assembly is scaffolded into 9 chromosomal pseudomolecules. Gene annotation of this assembly on Ensembl identified 20,502 protein coding genes.

Patella depressa↗
PubMed2006

Occupancy modeling of coverage distribution for whole genome shotgun DNA sequencing.

Expected-value models have long provided a rudimentary theoretical foundation for random DNA sequencing. Here, we are interested in improving characterization of genome coverage in terms of its underlying probability distributions. We find that the mathematical notion of occupancy serves as a good model for evolution of the coverage distribution function and reveals new insights related to sequence redundancy. Established concepts, such as "full shotgun depth," have been assumed invariant, but actually depend on project size and decrease over time. For most microbial projects, the full shotgun milestone should be revised downward by about 30%. Accordingly, many already-completed genomes appear to have been over-sequenced. Results also suggest that read lengths for emerging high-throughput sequencing methods must be increased substantially before they can be considered as possible successors to the standard Sanger method. In particular, gains in throughput and sequence depth cannot be made to compensate for diminished read length. Limits are well approximated by a simple logarithmic equation, which should be useful in estimating maximum coverage-based redundancy for future projects.

Algorithms↗
PubMed2025

The genome sequence of the Common Marble, Celypha lacunana (Denis & Schiffermüller, 1775) (Lepidoptera: Tortricidae).

We present a genome assembly from a male specimen of Celypha lacunana (Common Marble; Arthropoda; Insecta; Lepidoptera; Tortricidae). The genome sequence has a total length of 591.83 megabases. Most of the assembly (99.68%) is scaffolded into 27 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 17.21 kilobases.

Celypha lacunana↗
PubMed2005

Entamoeba histolytica: construction and applications of subgenomic databases.

Knowledge about the influence of environmental stress such as the action of chemotherapeutic agents on gene expression in Entamoeba histolytica is limited. We plan to use oligonucleotide microarray hybridization to approach these questions. As the basis for our array, sequence data from the genome project carried out by the Institute for Genomic Research (TIGR) and the Sanger Institute were used to annotate parts of the parasite genome. Three subgenomic databases containing enzymes, cytoskeleton genes, and stress genes were compiled with the help of the ExPASy proteomics website and the BLAST servers at the two genome project sites. The known sequences from reference species, mostly human and Escherichia coli, were searched against TIGR and Sanger E. histolytica sequence contigs and the homologs were copied into a Microsoft Access database. In a similar way, two additional databases of cytoskeletal genes and stress genes were generated. Metabolic pathways could be assembled from our enzyme database, but sometimes they were incomplete as is the case for the sterol biosynthesis pathway. The raw databases contained a significant number of duplicate entries which were merged to obtain curated non-redundant databases. This procedure revealed that some E. histolytica genes may have several putative functions. Representative examples such as the case of the delta-aminolevulinate synthase/serine palmitoyltransferase are discussed.

5-Aminolevulinate Synthetase↗
PubMed2024

The genome sequence of the English holly, Ilex aquifolium L. (Aquifoliaceae).

We present a genome assembly from an individual Ilex aquifolium (the English holly; Eudicot; Magnoliopsida; Aquifoliales; Aquifoliaceae). The genome sequence is 800.0 megabases in span. Most of the assembly is scaffolded into 20 chromosomal pseudomolecules. The assembled mitochondrial and plastid genomes have lengths of 538.43 kilobases and 157.52 kilobases in length, respectively.

Aquifoliales↗
PubMed2005

A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCR.

In a previous study, a molecular capsular type (MCT) prediction system for 51 Streptococcus pneumoniae serotypes was developed based on a combination of partial cpsA-cpsB sequencing and serotype(s)/group(s)-specific PCR. In this study, another 169 S. pneumoniae isolates were added to the existing database of 427 isolates, including representatives of all 39 serotypes not previously studied. In addition to the authors' own limited sequence data for all 90 serotypes, cpsA-cpsB sequence data published by the S. pneumoniae capsular loci-sequencing group (http://www.sanger.ac.uk/Projects/S_pneumoniae/CPS/) at the Sanger Institute or available from GenBank were incorporated into the database. All serotypes, except 25A, were represented by at least two isolates. The number of sequence types identified was 138, of which 110 corresponded to single conventional serotypes (CSs); of these, 57 were represented by two or more isolates. Twenty-six sequence types were shared by between two and four CSs. To resolve these shared cpsA-cpsB sequence types and increase the discriminatory power of our system, the genes encoding the capsular polysaccharide flippase (wzx) and polymerase (wzy) were annotated and 24 new serotype(s)/group(s)-specific PCRs targeting wzy and two targeting wzx were designed. Using both cpsA-cpsB sequencing and wzx/wzy PCR, MCT correctly predicted the CSs of 516 (73 %) and the serogroup of an additional 155 (22 %) of the 708 isolates evaluated. For 5 % of isolates, MCT could not distinguish between members of five serotype pairs (37 isolates) containing members of different serogroups. Although further study of the relationship between MCT and CS is needed, this system now allows serotype or serogroup identification of 95 % of S. pneumoniae isolates.

Bacterial Capsules↗
PubMed2025

The genome sequence of the solitary wasp, Cerceris ruficornis (Fabricius, 1793).

We present a genome assembly from a female specimen of Cerceris ruficornis (solitary wasp; Arthropoda; Insecta; Hymenoptera; Crabronidae). The genome sequence has a total length of 566.08 megabases, of which 65.35% is scaffolded into 14 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 18.07 kilobases. Gene annotation of this assembly on Ensembl identified 11,093 protein-coding genes.

Cerceris ruficornis↗
PubMed2025

The genome sequence of the Common Sheetweb Spider Linyphia triangularis (Clerck, 1757).

We present a genome assembly from a male Linyphia triangularis (Common Sheetweb Spider Arthropoda; Arachnida; Araneae; Linyphiidae). The genome sequence has a total length of 1,349.10 megabases. Most of the assembly (95.36%) is scaffolded into 13 chromosomal pseudomolecules, including the X 1 and X 2 sex chromosomes. The mitochondrial genome has also been assembled and is 15.31 kilobases in length.

Araneae↗
PubMed1997

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.

The complete DNA sequence of the yeast Saccharomyces cerevisiae chromosome IV has been determined. Apart from chromosome XII, which contains the 1-2 Mb rDNA cluster, chromosome IV is the longest S. cerevisiae chromosome. It was split into three parts, which were sequenced by a consortium from the European Community, the Sanger Centre, and groups from St Louis and Stanford in the United States. The sequence of 1,531,974 base pairs contains 796 predicted or known genes, 318 (39.9%) of which have been previously identified. Of the 478 new genes, 225 (28.3%) are homologous to previously identified genes and 253 (32%) have unknown functions or correspond to spurious open reading frames (ORFs). On average there is one gene approximately every two kilobases. Superimposed on alternating regional variations in G+C composition, there is a large central domain with a lower G+C content that contains all the yeast transposon (Ty) elements and most of the tRNA genes. Chromosome IV shares with chromosomes II, V, XII, XIII and XV some long clustered duplications which partly explain its origin.

Base Sequence↗
PubMed1981

A method for sequencing single-stranded cloned DNA in both directions.

A DNA sequencing method has been developed whereby DNA that has been cloned in a single-stranded bacteriophage vector can be sequenced from both ends. The method involves first making a minus-strand sense template from a single-stranded insert in the vector M13mp2 using a flanking primer, and then sequencing the synthesized template using the dideoxynucleotide termination method (Sanger et al., 1977, 1980) with a second primer. Special conditions are described under which the first primer is easily removed after making the template, and sequencing in the opposite direction can be done in the normal way (Sanger et al., 1980) without separating the double strands. This method renders it possible to read up to twice the amount of sequence data from a long insert and also to check short inserts by producing complementary sequence patterns.

Bacteriophage lambda↗
PubMed2024

The genome sequence of the Hoary Footman, Eilema caniola (Hübner, 1808).

We present a genome assembly from one female Eilema caniola (the Hoary Footman; Arthropoda; Insecta; Lepidoptera; Erebidae). The genome sequence is 781.7 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the W and Z sex chromosomes. The mitochondrial genome has also been assembled and is 15.42 kilobases in length. Gene annotation of this assembly on Ensembl identified 22,953 protein coding genes.

Eilema caniola↗
PubMed2025

The genome sequence of the Sandy Carpet moth, Perizoma flavofasciatum (Thunberg, 1792).

We present a genome assembly from a male specimen of Perizoma flavofasciatum (Sandy Carpet; Arthropoda; Insecta; Lepidoptera; Geometridae). The genome sequence has a total length of 369.30 megabases. Most of the assembly (99.88%) is scaffolded into 30 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 16.61 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,915 protein-coding genes.

Lepidoptera↗
PubMed2026

The genome sequence of Carex sylvatica Huds., 1762 (Poales: Cyperaceae).

We present a genome assembly of Carex sylvatica (Wood-sedge; Streptophyta; Magnoliopsida; Poales; Cyperaceae). The genome sequence has a total length of 363.24 megabases. Most of the assembly (99.79%) is scaffolded into 29 chromosomal pseudomolecules. The mitochondrial sequences have lengths of 1715.64, 74.78 and 77.37 kilobases and the plastid genome assembly has a length of 215.14 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.

Carex sylvatica↗