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The india-ink immunoreaction: a method for the rapid diagnosis of encephalitozoonosis.

Sera from 37 rabbits were assayed for antibodies against Encephalitozoon cuniculi (Nosema cuniculi) by the india-ink immunoreaction and the indirect fluorescent antibody tests: all animals seropositive to the former were also positive to the latter test. 27 of the rabbits were also tested for skin hypersensitivity and then autopsied. Animals positive to the skin test were also positive to the serological tests. At autopsy 18 of 22 rabbits positive in the immunological tests showed lesions typical of encephalitozoonosis. Sera from 200 rabbits originating from 6 institutes were assayed by the india-ink test: seropositive rabbits were found from all institutes (9.1 to 81.9% incidence), with serum titres ranging from 1:125 to 1:5000. The india-ink test appears to be a rapid and convenient method for diagnosis of encephalitozoonosis in rabbits.

Animals↗

Ultrastructural features of spironucleosis (hexamitiasis) in x-irradiated rat small intestine.

An alteration in the host response to the intestinal protozoan Spironucleus (Hexamita) muris was noted in x-irradiated rat small intestine. When few were present in the crypt lumina, the intestinal microvillar border was normal. However, when larger numbers were present, the microvilli were greatly reduced in numbers or entirely absent in some regions. The organism was identified in the base of the crypt lumen, in apical portions of mucous cells, and near the basement membrane. Spironucleus enclosed by digestive vacuoles occasionally appeared to be in the process of being extruded through the basement membrane. Not all intracellular protozoa were surrounded by such digestive vacuoles. Spironucleus was never seen in association with the digestive vacuoles of Paneth cells. Since it has been suggested that Paneth cells have a role as a fixed phagocyte, ingesting protozoa and other microorganisms, the results presented here suggest that this function may have been impaired in irradiated animals.

Animals↗

Haematology and pathology of captive southern grasshopper mice (Onychomys torridus).

A colony of Onychomys torridus was evaluated for the prevalence of infectious, parasitic and congenital diseases. Multiple limb defects including brachydactyly, syndactyly, and hemimelia were observed in offspring from the colony. The colony was parasitized by Ornithonyssus bacoti (tropical rat mites) on 2 occasions. No infectious diseases were detected. Normal haematologic values were determined in healthy adult animals.

Animals↗

Ciliate protozoa in the forestomach of the dromedary camel, (Camelus dromedarius), in Egypt, with description of a new species.

The composition of ciliates obtained from the forestomachs of eleven dromedary (one-humped) camels in Egypt was examined. As a result, eight genera containing 24 species with 11 forms were identified. Of them, one species was concluded to be new, then described as Dasytricha kabanii n. sp. This new species was clearly distinguished from D. ruminantium, the other species of the genus, by its lack of somatic cilia on the posterior one-fifth of the body surface. Entodinium nanellum and Epidinium ecaudatum f. caudatum were found in all camels examined. Although the percentage composition of respective species varied with the individual camel, the rate of Entodinium spp. was high in general. Total ciliate density in forestomach fluid was 1.9 x 10(5)/ml on average. Ciliate composition in Egyptian camels was similar to that in Bactrian camels, Camelus bactrianus, in China reported previously. However, more Entodinium species were detected from Egyptian camels than from Bactrian camels.

Animals↗

Identification of endotrypanum species from a sloth, a squirrel and Lutzomyia sandflies in ecuador by PCR amplification and sequencing of the mini-exon gene.

PCR amplification and nucleotide sequencing of the mini-exon gene revealed that four strains isolated from a sloth (Choloepus hoffmanni), a squirrel (Sciurus granatensis) and two sandflies (Lutzomyia hartmanni) in Ecuador were indistinguishable from Endotrypanum monterogeii. Another strain isolated from Lu. hartmanni showed the high sequence similarity to E. schaudinni. Since three of these strains have been previously identified as Leishmania (Viannia) equatorensis, the results demonstrate that L. (V.) equatorensis is genetically closely related to the genus Endotrypanum. The present study also indicates that Endotrypanum species are distributed in arboreal animals and sandflies in Ecuador, and that mini-exon gene amplification is useful for epidemiological studies of Leishmania and Endotrypanum in the New World.

Animals↗