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At least 487 records · Page 27Linked to original sources

PubMed2004

Use of self-etching adhesives to seal resected apices.

The purpose of this study was to evaluate the sealing ability of four self-etching adhesives placed over blood-contaminated/uncontaminated resected root apices without root-end preparation. Extracted maxillary incisors and canines were randomly divided into four groups of 10 teeth each. After canal preparation and resection of the apex, four self-etching adhesives (Clearfil SE Bond, One-Up Bond F, Unifil Bond, and ABF) were applied over the control and contaminated surfaces. The roots were then subjected to 15 cm of water pressure to simulate periapical microleakage stress. Data were analyzed using a two-way repeated measures, ANOVA. Positive and negative controls responded as expected. Statistical analysis indicated that there were no significant differences in the sealing effectiveness among the adhesives applied to contaminated or uncontaminated groups. All contaminated groups had significantly higher leakage (p < 0.003) than their uncontaminated pairs.

Analysis of Variance↗

PubMed2000

Correlation between remaining length of root canal fillings after immediate post space preparation and coronal leakage.

The seal provided by root canal fillings after post space preparation was studied using a pressure-driven radioactive tracer assay. The coronal part of root canal fillings was immediately removed, using a hot plugger, to a remaining length of either 3, 5, 7, or 9 mm. Intact root canal fillings of 14 mm served as control. Application of air pressure of 130 mm Hg to the tracer solution drove it through the fillings and into phosphate-buffered saline surrounding the apex. Leakage gradually increased for 28 days, and differences in the leakage through 3 to 9 mm fillings were demonstrated. In a passive system by which an additional group of teeth were tested none of these differences could be detected. It was concluded that: (a) root canal fillings of 3, 5, and 7 mm have an inferior seal, compared with that of an intact filling; (b) the sealing is proportional to the length of the remaining filling; and (c) a passive system is unable to detect these differences, even when conducted for as long as 28 days.

Analysis of Variance↗

PubMed2004

The accuracy of electronic working length determination.

AIM: To determine in vivo the accuracy of two impedance quotient apex locators under clinical conditions. METHODOLOGY: Electronic working length determination was carried out before extraction in 79 human teeth with 93 root canals. In 51 root canals, the determination was performed using the apex locator Justy II(R) (Hager & Werken, Duisburg, Germany); in 42 canals, the apex locator Endy 5000(R) (Loser, Leverkusen, Germany) was used. A root canal instrument was fixed at working length with composite material prior to extraction followed by the exposure of a radiograph. After histological preparation of the apical region, the teeth were examined under a light microscope. The distance of the file tips to the target intervals 'minor foramen - major foramen' and 'apical canal constriction' was determined. These values were compared with the calculated working lengths, determined by radiographic assessment. The data were statistically analysed by a paired t-test. RESULTS: For both apex locators and both target intervals, no significant differences between the electronic and radiographical assessments were recorded. The probability of determining the area between minor and major foramen was 82.4% for Justy II and 81% for Endy 5000. However, accurate determination of the apical constriction was only successful in 51% (Justy II) and 64.3% (Endy 5000) of canals. Variation of the inaccurate measurements was higher for Endy 5000 than for Justy II. CONCLUSIONS: Under clinical conditions, it is possible to determine the region between the minor and major apical foramen with electronic length measuring devices (ELD). However, use of these devices does not result in precise determination of the apical constriction.

Adult↗

PubMed2002

The accuracy of the Neosono Ultima EZ apex locator using files of different alloys: an in vitro study.

The purpose of this study was to compare the precision of one of the new generation of root canal measuring devices, Neosono Ultima EZ, while using files manufactured of different alloys. Fifty-four root canals of extracted teeth were chosen. They were placed in special tubes with roots immersed in 2 percent agar with phosphate buffered saline. The device was used to locate the apex of each canal in wet conditions at the zero digital reading, first using a stainless steel file and then using a nickel-titanium file. These values were compared to the actual lengths obtained by measuring the distance of the coronal reference point to the apical opening with a size 10 file minus 0.5 mm. The accuracy of the device was 94 percent with nickel-titanium files and 91 percent with stainless steel. No significant difference was noted between the results for either file. The accuracy of the Neosono Ultima EZ in wet conditions exceeded 90 percent regardless of the alloy used.

Dental Alloys↗

PubMed2006

Anatomical redesign for the treatment of dens invaginatus type III with open apexes: a literature review and case presentation.

BACKGROUND: Dens invaginatus is a rare dental anomaly that may give rise to many complex anatomical forms. The complexity of the internal anatomy may create challenges for the complete removal of the diseased pulpal tissue and the subsequent sealing of the canal system. CASE DESCRIPTION: The authors discuss the modification of the internal anatomy under the operating microscope, allowing the clinician better access to treat predictably the canal system with conventional or alternative techniques. CONCLUSION AND CLINICAL IMPLICATIONS: Considering the anatomical variations and the challenges that a dens invaginatus may present, a practitioner may consider a modification of the internal anatomy of the canal system to gain better access for proper instrumentation, disinfection and sealing of the root canal system using conventional or contemporary techniques.

Adolescent↗

PubMed2005

The effect of the renewal of calcium hydroxide paste on the apexification and periapical healing of teeth with incomplete root formation.

AIM: To evaluate the influence of renewing calcium hydroxide paste on apexification and periapical healing of teeth in dogs with incomplete root formation and previously contaminated canals. METHODOLOGY: Forty premolars from four 6-month-old dogs were used. After access to the root canals and complete removal of the pulp, the canal systems remained exposed to the oral environment for 2 weeks. Canal preparation was then carried out using Hedströem files, under irrigation with 1% sodium hypochlorite, 1 mm short of the radiographic apex. After drying, the canals of one premolar in each dog were left empty (group 4-control), and those of the other nine teeth in each animal were filled with a calcium hydroxide-propylene glycol paste. All teeth were restored with reinforced zinc oxide cement (IRM) or IRM and amalgam (group 4). The paste was renewed and the teeth restored again 1 week later. Then, the nine teeth in each animal were divided into three experimental groups: group 1 - paste not changed; group 2 - paste renewed every 4 weeks for 5 months; and group 3 - paste renewed after 3 months had elapsed. The teeth were restored with IRM and amalgam (groups 1 and 3) or IRM (group 2). The animals were killed 5 months later, and blocks of the teeth and surrounding tissues were submitted to histological processing. The sections were studied to evaluate six parameters: apical calcified tissue barrier, inflammatory reaction, bone and root resorption, paste extrusion and microorganisms. Results of experimental groups were analysed by Kruskal-Wallis nonparametric tests and by the test of proportions. The critical value of statistical significance was 5%. RESULTS: Significant differences (P < 0.05) were found in relation to the presence of bone resorption and paste in the periradicular area, the formation of a calcified tissue barrier at the apex, and the intensity of the apical inflammatory reaction. Bone resorption was more evident in group 1 (medicament not changed), and the presence of paste in the periodontal tissues was more common in groups 2 and 3. Renewal of the paste reduced the intensity of the inflammatory reaction (groups 2 and 3), but the formation of apical calcified tissue was more noticeable in the teeth where the paste had not been renewed. CONCLUSIONS: Replacement of calcium hydroxide paste was not necessary for apexification to occur, however, it did reduce significantly the intensity of the inflammatory process. Monthly renewal of calcium hydroxide paste reduced significantly the occurrence of apexification.

Animals↗

PubMed2001

In vitro apical leakage of system B compared with other filling techniques.

A fluid filtration system was used to compare the apical microleakage of roots filled with the system B (Analytic Technology), single-cone technique, lateral condensation, vertical condensation, and Thermafil (Caulk-Dentsply). After preparation of the canal system with a Profile (Caulk-Dentsply) five groups of 10 single-rooted teeth were randomly filled according to 1 of the 5 techniques. Phosphate-buffered saline was forced under a 15 cm H2O pressure, through the apex, toward the coronal part of the root. The filtration rate in L s(-1) Kpa(-1) was recorded 24 h after filling and after 1-month storage in phosphate-buffered saline. The 24 h results showed that the single cone technique provided the highest leakage (p = 0.001). At 1-month system B, Thermafil and vertical condensation had less leakage than the two other techniques (p = 0.0001): lateral condensation produced moderate apical leakage, whereas the single cone technique showed the highest leakage. Regardless of the technique the apical leakage increased after 1-month storage (p = 0.001 or p = 0.0001, depending on the technique).

Analysis of Variance↗

PubMed1997

Warm vertical compaction sequences in relation to gutta-percha temperature.

The aim of this study was to analyze warm vertical compaction sequences in relation to the temperature variations of gutta-percha. Twenty-four maxillary incisors were obturated according to two different instrumentation techniques: Compaction and heating were performed with either the monomanual classical or bimanual method. To evaluate gutta-percha temperature, thermocouples (TC) were introduced into channels bored through the dentin into the root canal system or fixed on the root surface at 8 (TC8), 4 (TC4), 2 (TC2), and 0 mm (TC0) from the apex. Maximal temperature was 118 degrees C for TC8, 52 degrees C for TC4, and 44 degrees C for TC2 and TC0. At TC0, the temperature remained above 42.9 degrees C for 10 +/- 5 s. These results show the importance of heating down to a distance of 7 mm to ensure a significant increase in apical gutta-percha temperature. They further indicate that the last compaction should last more than 15 s to avoid detrimental cooling and dimensional variations. The principal differences between the monomanual and bimanual methods were the compaction time (5.07 +/- 0.2 min vs 4.02 +/- 0.3 min) and the number of compactions (8 +/- 1 vs 12 +/- 2).

Analysis of Variance↗

PubMed2004

Apical microleakage of root-end cavities prepared by CO2 laser.

This in vitro study in extracted human teeth was designed to compare the degree of dye penetration of root-end cavities prepared by CO2 laser, ultrasonic retrotip, or rotary instrument. Eighty-two human anterior teeth were prepared and obturated with laterally condensed gutta-percha and sealer. Root-end resection was performed at 90 degrees, 3 mm from apex. Root-end cavities were prepared by CO2 laser beam, ultrasonic retrotips, or bur and were filled with amalgam. The teeth were isolated and immersed in basic fuchsin at 36 degrees C for 48 hours. The roots were split longitudinally and examined by a stereomicroscope. Leakage was quantified by linear measurement of dye penetration with the aid of image-analyzing software. Apical leakage in the CO2 laser group was significantly less than in the ultrasonic retrotip and bur groups. The difference between the ultrasonic and bur groups was insignificant. Based on the results of this in vitro study, application of CO2 laser for root-end cavity preparation appears to decrease the apical leakage.

Dental Cavity Preparation↗

PubMed2002

EM evaluation of bacterial biofilm and microorganisms on the apical external root surface of human teeth.

The aim of this study was to evaluate the presence of bacterial biofilm on the external surface of the root apex in teeth with pulp necrosis, with and without radiographically visible periapical lesions, and in teeth with a vital pulp. Twenty-one teeth were extracted, eight with pulp necrosis and periapical lesions, eight with pulp necrosis without radiographically visible periapical lesions, and five with a vital pulp. The roots were sectioned, and the root apexes (+/- 3 mm) were processed for scanning electron microscope evaluation. The surface of the apical root was evaluated for the presence of microorganisms, root resorption, and biofilm. There were no microorganisms on the apical root surface of either teeth with pulp vitality or with pulp necrosis with no radiographically visible periapical lesions. Microorganisms were always present in teeth with pulp necrosis and radiographically visible periapical lesions. These included cocci, bacilli, and filaments and the presence of an apical biofilm. Apical biofilm is clinically important because microbial biofilms are inherently resistant to antimicrobial agents and cannot be removed by biomechanical preparation alone. This may cause failure of endodontic treatment as a consequence of persistent infection.

Biofilms↗

PubMed1999

Changes in root canal morphology in simulated curved canals over-instrumented with a variety of stainless steel and nickel titanium files.

The purpose of this project was to observe the amount of apical and mid-curve transportation produced by a range of nickel titanium (NiTi), titanium alloy and stainless steel (SS) files. Tests were carried out in simulated curved root canals produced in epoxy resin blocks. Seven commercially available file types were tested using sizes 15 to 40. Instrumentation was carried out to 1 mm beyond the apex. Changes in canal dimensions were measured at 10x magnification under a shadowgraph. There were substantial differences in the amount and the pattern of apical and mid-curve transportation produced. The amount of transportation increased with each subsequent size of file. Under the same conditions, nickel titanium files produced significantly less transportation than stainless steel files. The least apical transportation was obtained with the NiTi Mity Turbo and the most by the SS K file and SS Hedstrom file. The least mid-curve transportation was produced by the NiTi Mity Turbo and the most by the SS Hedstrom file.

Alloys↗

PubMed2004

Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment.

OBJECTIVE: In this study, we aimed to investigate the occurrence of several microbial species in cases of failed endodontic therapy by means of the polymerase chain reaction (PCR). Study design Root canal samples were taken from 22 root-filled teeth with persistent periradicular lesions selected for re-treatment. DNA was extracted from the samples and analyzed for the presence of 19 microbial taxa by using the polymerase chain reaction. RESULTS: All samples were positive for at least 1 of the target microbial species. Enterococcus faecalis was the most prevalent species-detected in 77% of the cases. The other most prevalent species were Pseudoramibacter alactolyticus (52%), Propionibacterium propionicum (52%), Dialister pneumosintes (48%), and Filifactor alocis (48%). Candida albicans was found in 9% of the samples. The mean number of species in samples filled up to 2 mm short of the radiographic apex was 3 (range, 1-5), whereas cases in which the filling was greater than 2 mm from the apex yielded a mean of 5 species (range, 2-11). This difference was statistically significant (P <.05). CONCLUSIONS: Microorganisms occurred in all cases of root-filled teeth associated with periradicular lesions, which lends strong support to the assertion that treatment failures are rather of infectious etiology, caused by persistent or secondary intraradicular infections. E faecalis was the most prevalent species, followed by 4 other anaerobic species: P. alactolyticus, P. propionicum, D. pneumosintes, and F. alocis. All examined samples harbored at least 1 of the following gram-positive bacterial species: E. faecalis, P. alactolyticus, or P. propionicum.

Adult↗

PubMed2007

Periapical surgery and the maxillary sinus: radiographic parameters for clinical outcome.

OBJECTIVE: To find out whether conventional periapical radiographs can be used to determine the risk of creating an oroantral communication (OAC) while performing periapical surgery on maxillary premolars and molars. STUDY DESIGN: One hundred thirteen periapical radiographs of maxillary premolars and molars with periapical radiolucencies indicating chronic apical periodontitis were retrospectively analyzed and classified. The surgery reports were evaluated for occurrence of perforation of the maxillary sinus and postoperative complications. RESULTS: Perforation of the sinus membrane (also referred to as the Schneiderian membrane) occurred in 12 cases (9.6%). Exposure of the membrane without rupture occurred in 15 cases (12%). It was found that the distance between the apex or the periapical lesion and the sinus floor did not serve as a predictor of a possible sinus membrane rupture. On the other hand, if the radiograph showed a distinct distance between the lesion and the sinus floor, there was an 82.5% probability that OAC would not occur. Additionally, a blurred radiographic outline of the periapical lesion did not indicate an increased risk of sinus membrane rupture. CONCLUSION: Conventional periapical radiographs cannot be used as predictors for perforation of the maxillary sinus during periapical surgery. However, radiographs with a specific distance between the periapical lesion and the sinus floor point toward a very low risk of accidental sinus perforation during periapical surgery.

Adult↗

PubMed2002

The effects of sodium hypochlorite concentrations on the accuracy of an apex locating device.

This study aimed to evaluate Root ZX accuracy with different NaOCl concentrations and to observe effects of the alginate model when NaOCl was used in the canal during electronic measurements. Fifty extracted single-rooted, single-canal human teeth were used in five experimental groups. NaOCl concentrations of 5.25%, 2.65%, 1.00%, and 0.50%, respectively, were injected into the root canals of teeth in four groups and distilled water in control group 5. After measuring root lengths with Root ZX, the files were locked in place. There were no statistically significant differences among all the groups (p > 0.95) when evaluating the distance of the file tips and apical constriction or for the distance from file tip to apical foramen. Root ZX could be used in root canal length measurements with various NaOCl concentrations. Designation of apical constriction needs more care. The alginate model could be used for in vitro electronic measurements with various NaOCl concentrations.

Alginates↗

PubMed2006

The role of apical size determination and enlargement in the reduction of intracanal bacteria.

The master apical preparation size in root canal therapy is debatable despite considerable research. The present study compared file sizes that bind at the apex before and during crown-down preparation and assessed the relation between apical size and extent of intracanal bacterial load. There were 100 single-rooted teeth biomechanically prepared after inoculation with Enterococcus faecalis. Canals were preflared, and apical size was ascertained by the first file to bind (FAB) at the working length (WL). During crown-down preparation, the first crown-down file to reach the apex during instrumentation was noted (CDF). Teeth were then divided into three master apical file size groups of CDF + 1, CDF + 2, and CDF + 3. Positive controls were inoculated postinstrumentation, whereas negative controls were instrumented without inoculation (n = 5). The samples were then cultured for intracanal bacterial counts. Fifteen samples and four controls were analyzed under SEM. The data were analyzed using ANOVA, Student's t-test, and chi(2) tests. The CDF was demonstrated to be an average of four file sizes larger than the FAB (p < 0.05). There was a significant increase in the number of samples with negative cultures from CDF + 1 to CDF + 3. SEM observation revealed bacteria on dentinal walls and in tubules even in most negative canal cultures.

Analysis of Variance↗

PubMed2003

Displaced calcium hydroxide paste causing inferior alveolar nerve paraesthesia: report of a case.

A patient presented with an intraoral red, painful, and hard swelling in the lower right jaw. Radiographs showed a 2 x 1 cm area of radiopaque material surrounding the apex of the second premolar. The material, according to the patient's dentist, was calcium hydroxide paste used as a temporary dressing material in the root canal. The patient developed paraesthesia in her lower lip probably due to a neurotoxic effect caused by calcium hydroxide. The foreign material was surgically excavated from the spongious bone, directly adjacent to the nerve, and the patient later regained her sensation in the lip. A histopathological analysis revealed necrosis, deposits of foreign bodies, and inflammatory cells and foreign-body giant cells. This report illustrates the toxicity and adjacent clinical symptoms of calcium hydroxide paste when displaced into bone tissue close to the alveolar inferior nerve. It also demonstrates the benefits of removing such displaced material before symptoms progress.

Calcium Hydroxide↗

PubMed2001

Shaping ability of Hero 642 rotary nickel-titanium instruments and stainless steel hand K-Flexofiles in simulated curved root canals.

OBJECTIVE: The purpose of this study was to compare the shaping ability of Hero 642 rotary nickel-titanium instruments with that of stainless steel hand K-Flexofiles. STUDY DESIGN: Simulated 28 degrees and 35 degrees curved canals were prepared with Hero 642 instruments by using a crown-down preparation and with K-Flexofiles by using a reaming working motion (n = 24 canals in each case). All canals were sequentially prepared up to size 35. Preinstrumental and postinstrumental images were taken, and assessment of the canal shapes was completed with a computer image analysis program. Material removal was measured at 20 measuring points, beginning 1 mm away from the apex. In addition, preparation time, changes of working length, and instrument failures were recorded. RESULTS: In comparison with stainless steel K-Flexofiles, rotary Hero 642 instruments achieved better canal geometry and showed less canal transportation, both in canals with 28 degrees curves and in canals with 35 degrees curves. Three Hero instruments separated, whereas none of the K-Flexofiles separated. In 28 degrees curved canals, Hero instruments were significantly faster (P <.01) and maintained working distance significantly better (P <.05) than K-Flexofiles. These differences were insignificant in 35 degrees curved canals. CONCLUSION: Hero 642 instruments prepared curved canals rapidly, without change in working length and with only minimal transportation toward the outer aspect of the curve.

Dental Alloys↗

PubMed2000

Immunolocalization of macrophages and transforming growth factor-beta 1 in induced rat periapical lesions.

Apical periodontitis was induced in Wistar rats by exposing the pulp chamber of right mandibular first molars to the oral environment. Animals were killed 0, 5, 10, 15, 20, 30, 60, and 80 days after lesion induction. Microradiographic and automated image analysis showed that the lesions expanded significantly in a time-dependent manner from day 0 to day 20 (0.039 mm2/day, p < 0.05, active phase) and stabilized thereafter (chronic phase). A linear regression test revealed a positive correlation between the numbers of ED-1 positive macrophage per microscopic high power field and the periapical lesion size during the active phase (r = 0.98, p < 0.01). Immunohistochemical studies showed that transforming growth factor-beta 1 positive macrophages distributed around the root apex and areas showing bone resorption during active lesion phase, whereas TGF-beta 1-positive osteoblasts were detected during the chronic stage (days 30, 60, and 80 after pulp exposure). Histologically TGF-beta 1 positive osteoblasts possessed a large, round nucleus as well as an abundant cytoplasm and located in close vicinity to areas exhibiting reparative bone formation. These results suggest that macrophages may play important role(s) in the initiation and development of periapical lesions and TGF-beta 1 may play dual roles in both bone resorption and deposition in induced rat periapical lesions.

Acute Disease↗