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PubMed2001

[Fatal fulminant acute amebic colitis in metropolitan France].

BACKGROUND: Emergency care is required for patients presenting dysentery and fever. CASE REPORT: A 65-year old patient living in metropolitan France was hospitalized for watery diarrhea that had progressively worsened over the last month. Rectal bleeding was also noted. Serology tests at admission, together with parasitology examination of the fecal matter and colonoscopy led to the diagnosis of intestinal amebiasis a few hours before the patient's rapid death. Serology was positive for amebas and the diagnosis was confirmed by pathology examination of the colonoscopy biopsies. DISCUSSION: The diagnosis of amebiasis should be entertained even in Europe when there is no history of travel to endemic areas in patients who develop suggestive manifestations. Identification of amebas in the fecal matter and serology tests provide certain diagnosis. Clinicians should recall that rapidly fatal outcome is not uncommon in severe forms. Emergency care is mandatory.

Aged↗
PubMed1992

Interaction of various Entamoeba histolytica strains with human intestinal cell lines.

The interaction of four pathogenic and three nonpathogenic E. histolytica strains with two human intestinal cell lines (Caco-2 and HT-29) was examined. The adherence of pathogenic and nonpathogenic E. histolytica to these cells was similar, indicating that defective adherence to intestinal cells is not a common feature of nonpathogenic strains. The addition of different carbohydrates confirmed the importance of the galactose-binding lectin of E. histolytica in binding to these intestinal cells. On the other hand, only virulent E. histolytica strains damaged monolayers of intestinal cells. The results indicate that Caco-2 cells and differentiated HT-29 cells are useful models for research of intestinal amebiasis.

Animals↗
PubMed1992

Inhibition of adhesion process mediated by anti-Entamoeba histolytica specific monoclonal IgA antibodies.

Adhesion of trophozoites to target cells in the host intestine is the first of three consecutive steps (adherence, cytolytic effect and phagocytosis) involved in the invasion of colonic tissue by E. histolytica. To investigate the possible participation of the local secretory immune response in the interference with this early host-parasite relationship, we produced IgA monoclonal anti-E. histolytica antibodies to test their capacity for blocking the adhesion process in vitro (MDCK and HT-29 cell lines) and in situ (colonic mucosa from Balb/c mice and gerbils). Results demonstrate that the monoclonal antibodies tested inhibited trophozoite adherence both in vitro and in situ. This suggests that the local antibody response may play an important role in protection against the invasive process in intestinal amebiasis.

Adenocarcinoma↗
PubMed1992

[Pediatric AIDS: a report of the first fatal Cuban pediatric case].

The case of a Cuban child with AIDS acquired by perinatal transmission is reported. Thirteen days after birth, the child had chronic diarrhoeas affecting its pondostatural development. It was hospitalized many times due to recurrent respiratory processes, in one of which Pneumocystis carinii was detected. Oral candidiasis, cryptosporidiosis and intestinal amebiasis in faeces were also diagnosed. It died with generalized tonic-clonic convulsions and bradypnea. At autopsy, the direct death cause was endocranial hypertension due to unspecific sub-acute viral meningitis.

AIDS-Related Opportunistic Infections↗
PubMed1978

The development and standaridization of an ELISA method for the detection of Entamoeba histolytica antigens in fecal samples.

Recognizing the difficluties associated with the microscopic detection and identification of Entamoeba histolytica in fecal samples, an immunochemical method of detection of E. histolytica antigens in fe-es has been developed. For a number of reasons, e.g., the known fragility of E. histolytica trophozoites, it was felt that intestinal amebiasis would be associated with detectable levels of E. histolytica anti-ens in feces from infected patients. A technique for harvesting amebic antigens in fecal samples was developed. An enzyme labeled antibody technique was used to visualize the harvested antigens. The inherent problems of performing any immunochemical assay in fecal samples have been addressed. The enzyme labeled antibody method is simple and is designed to be more sensitive than microscopic stool examination methods. Preliminary results indicate that the method is specific and sensitive.

Animals↗
PubMed1990

[Cardiotoxicity of emetine].

Emetine is still an important agent for the treatment of invasive intestinal amebiasis and amebic liver abscess. Its most relevant side effect is its well documented cardiotoxicity. The authors review the hypotheses that have been suggested concerning the mechanism of this cardiotoxic action.

Animals↗
PubMed1979

[The diagnosis of amebic liver abscesses. A study of 107 cases (author's transl)].

We have investigated the serum antibodies of 107 patients with extra-intestinal amebiasis and 72 control subjects without demonstrable infection with E. histolytica. It was shown that by means of countercurrent immuno-electrophoresis and indirect hemagglutination scintigraphy and taking into consideration the clinical picture, the diagnosis of extraintestinal amebiasis can be made with the necessary high degree of probability.

Antibodies↗
PubMed1987

[Therapeutic effectiveness of CT-controlled drainage puncture in amebic abscesses of the liver].

Amebic liver abscesses are the most frequent clinical manifestations of the extra-intestinal amebiasis and have to be considered as a serious complication in the course of disease. The abscesses have by their risk of rupturing and bacterial superinfection quoad vitam an unfavourable prognosis. CT-guided relief punctures can, as it is demonstrated by two cases of critical amebic abscesses, effectively improve the therapy with systemic amebicides, prevent imminent rupture and contribute to the healing of the abscesses. Thus a surgical treatment of the abscess was not necessary.

Drainage↗
PubMed1979

[Indirect immunoperoxidase technic in the biological diagnosis of human parasitic disease].

Two serological techniques, immunoperoxidase (IP) and immunofluorescence (IFI), were used for the detection of specific antibodies in 60 sera from patients with schistosomiasis, in 44 sera from patients with hydatidosis, and 28 sera from patients with intestinal amebiasis or liver amebic abscess. The IP test correlated well with IFI. The advantage of IP technique compared to IFI are that it is more rapid, less laborious and is performed without technical equipment.

Dysentery, Amebic↗
PubMed1980

Antiparasitic drugs.

Antiprotozoan drugs of choice include: chloroquine for malaria; diiodohydroxyquin for asymptomatic intestinal amebiasis; metronidazole for acute amebic colitis, extraintestinal amebiasis and trichomoniasis; quinacrine for giardiasis; quinine-pyrimethamine-sulfadiazine for chloroquine-resistant falciparum malaria, and trimethoprim-sulfamethoxazole for pneumocystis pneumonia. Anthelmintic drugs of choice include: mebendazole for roundworm, pinworm, whipworm and hookworm infections; niclosamide for tapeworm infections, and thiabendazole for trichinosis.

Anthelmintics↗
PubMedDate not supplied

[Evaluation of the tolerance and efficiency of quinfamide, a new intraluminal amebicide, in man (one day treatment). Double blind study].

A new intraluminal amebicide (Quinfamide) was tested to assess its effectivity and tolerance for treatment of non-dysenteric intestinal amebiasis. The drug was administered to three groups of ten patients each, whom received 300, 600 and 1 200 mg. on a 24 hours schedule. Another group of ten patients received Teclozan as control drug. Diagnosis and results were judged by rectosigmoidoscopy before, 15 and 30 days after treatment. In addition, microscopic investigation of ameba was performed in freshly passed stools, before, and after 8, 15 and 30 days of treatment. Success after treatment with the three doses of Quinfamide was obtained in 89.2% of the cases. Side reactions were clinically non-significant. More experience is needed before the effectivity of the drug is stablished.

Acetamides↗
PubMed1994

[Clinical diagnosis of malaria and amoebiasis].

In all patients with fever after returning from tropical countries malaria must be excluded. Amebic liver abscess is an important differential diagnosis, especially in patients with pain in the right epigastrium. In all patients with diarrhea after returning from tropical countries amebic colitis is an important differential diagnosis. The diagnosis of malaria is based on microscopical demonstration of the parasite in blood slides. In patients with Plasmodium falciparum malaria the extent of organ complications has to be checked. The diagnosis of intestinal amebiasis rests on parasitological stool examination. Amebic liver abscess is diagnosed by demonstration of the liver abscess in imaging techniques and verification by finding specific antibodies.

Amebiasis↗