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PubMed2003

The J-domain of Hsp40 couples ATP hydrolysis to substrate capture in Hsp70.

The Escherichia coli Hsp40 DnaJ uses its J-domain to target substrate polypeptides for binding to the Hsp70 DnaK, but the mechanism of J-domain function has been obscured by a substrate-like interaction between DnaJ and DnaK. ATP hydrolysis in DnaK is associated with a conformational change that captures the substrate, and both DnaJ and substrate can stimulate ATP hydrolysis. However, substrates cannot trigger capture by DnaK in the presence of ATP, and substrates stimulate a DnaK conformational change that is uncoupled from ATP hydrolysis. The role of the J-domain was examined using the fluorescent derivative of a fusion protein composed of the J-domain and a DnaK-binding peptide. In the absence of ATP, DnaK-binding affinity of the fusion protein is similar to that of the unfused peptide. However, in the presence of ATP, the affinity of the fusion protein is dramatically increased, which is opposite to the decrease in DnaK affinity typically exhibited by peptides. Binding of a fusion protein that contains a defective J-domain is insensitive to ATP. According to results from isothermal titration calorimetry, the J-domain binds to the DnaK ATPase domain with weak affinity (K(D) = 23 microM at 20 degrees C). The interaction is characterized by a positive enthalpy, small heat capacity change (DeltaC(p)= -33 kcal mol(-1)), and increasing binding affinity for increasing temperatures in the physiological range. In conditions that support binding of the J-domain to the ATPase domain, the J-domain accelerates ATP hydrolysis and a simultaneous conformational change in DnaK that is associated with peptide capture. The defective J-domain is inactive, despite the fact that it binds to the DnaK ATPase domain with higher than wild-type affinity. The results are most consistent with an allosteric mechanism of J-domain action in which the J-domain couples ATP hydrolysis to peptide capture by accelerating ATP hydrolysis and delaying DnaK closure until ATP is hydrolyzed.

Adenosine Triphosphate↗

PubMed2001

Capture of gas-phase arsenic oxide by lime: kinetic and mechanistic studies.

Trace metal emission from coal combustion is a major concern for coal-burning utilities. Toxic compounds such as arsenic species are difficult to control because of their high volatility. Mineral sorbents such as lime and hydrated lime have been shown to be effective in capturing arsenic from the gas phase over a wide temperature range. In this study, the mechanism of interaction between arsenic oxide (As2O3) and lime (CaO) is studied over the range of 300-1000 degrees C. The interaction between these two components is found to depend on the temperature; tricalcium orthoarsenate (Ca3As2O8) is found to be the product of the reaction below 600 degrees C, whereas dicalcium pyroarsenate (Ca2As2O7) is found to be the reaction product in the range of 700-900 degrees C. Maximum capture of arsenic oxide is found to occur in the range of 500-600 degrees C. At 500 degrees C, a high reactivity calcium carbonate is found to capture arsenic oxide by a combination of physical and chemical adsorption. Intrinsic kinetics of the reaction between calcium oxide and arsenic oxide in the medium-temperature range of 300-500 degrees C is studied in a differential bed flow-through reactor. Using the shrinking core model, the order of reaction with respect to arsenic oxide concentration is found to be about 1, and the activation energy is calculated to be 5.1 kcal/mol. The effect of initial surface area of CaO sorbent is studied over a range of 2.7-45 m2/g using the grain model. The effect of other major acidic flue gas species (SO2 and HCl) on arsenic capture is found to be minimal under the conditions of the experiment.

Adsorption↗

PubMed2005

Fluidized bed combustion systems integrating CO2 capture with CaO.

Capturing CO2 from large-scale power generation combustion systems such as fluidized bed combustors (FBCs) may become important in a CO2-constrained world. Using previous experience in capturing pollutants such as SO2 in these systems, we discuss a range of options that incorporate capture of CO2 with CaO in FBC systems. Natural limestones emerge from this study as suitable high-temperature sorbents for these systems because of their low price and availability. This is despite their limited performance as regenerable sorbents. We have found a range of process options that allow the sorbent utilization to maintain a given level of CO2 separation efficiency, appropriate operating conditions, and sufficiently high power generation efficiencies. A set of reference case examples has been chosen to discuss the critical scientific and technical issues of sorbent performance and reactor design for these novel CO2 capture concepts.

Adsorption↗

PubMed2005

Electron attachment step in electron capture dissociation (ECD) and electron transfer dissociation (ETD).

We have made use of classical dynamics trajectory simultions and ab initio electronic structure calculations to estimate the cross sections with which electrons are attached (in electron capture dissociation (ECD)) or transferred (in electron transfer dissociation (ETD)) to a model system that contained both an S-S bond that is cleaved and a -NH(3)(+) positively charged site. We used a Landau-Zener-Stueckelberg curve-crossing approximation to estimate the ETD rates for electron transfer from a CH(3)(-) anion to the -NH(3)(+) Rydberg orbital or the S-S sigma* orbital. We draw conclusions about ECD from our ETD results and from known experimental electron-attachment cross sections for cations and sigma-bonds. We predict the cross section for ETD at the positive site of our model compound to be an order of magnitude larger than that for transfer to the Coulomb-stabilized S-S bond site. We also predict that, in ECD, the cross section for electron capture at the positive site will be up to 3 orders of magnitude larger than that for capture at the S-S bond site. These results seem to suggest that attachment to such positive sites should dominate in producing S-S bond cleavage in our compound. However, we also note that cleavage induced by capture at the positive site will be diminished by an amount that is related to the distance from the positive site to the S-S bond. This dimunition can render cleavage through Coulomb-assisted S-S sigma* attachment competitive for our model compound. Implications for ECD and ETD of peptides and proteins in which SS or N-C(alpha) bonds are cleaved are also discussed, and we explain that such events are most likely susceptible to Coulomb-assisted attachment, because the S-S sigma* and C=O pi* orbitals are the lowest-lying antibonding orbitals in most peptides and proteins.

Algorithms↗

PubMed1996

Cells involved in the capture of nanoparticles in hematopoietic organs.

The affinity of nanoparticles for hematopoietic organs could be valuable for the targeting of certain stimulating factors to those tissues, but this affinity should also be taken into account in the toxicological evaluation of those carriers, especially when they are loaded with antimitotic compounds such as doxorubicin. However, the cells responsible for the capture of the nanoparticles and their localization in these organs is an important point to know before trying to modulate the nanoparticle's tissue distribution. Thus, we have studied, in this paper, the capture, the localization, and the retention in the bone marrow and in the spleen of biodegradable poly(isohexyl cyanoacrylate) nanoparticles as well as of nonbiodegradable polystyrene nanoparticles. The histological localization of these nanoparticles has been completed by cytological localization with a method used in cytochemistry for the evaluation of intracellular accumulation of various substances, such as iron deposits in bone marrow sideroblasts. These data indicate that, in the bone marrow, after a quick passage through the endothelium, nanoparticles were dispersed throughout in the tissue and captured by all types of phagocytizing cells. In the spleen, nanoparticles were mainly localized in large angular capturing cells in the marginal zone of the lymphoid follicles.

Animals↗

PubMed2000

Adrenal cortex and stomach lesions associated with stress in wild male African green monkeys (Cercopithecus aethiops) in the post-capture period.

The objective of this study was to look for early pathological changes in stress target organs, adrenal glands, and stomachs in captured wild African green monkeys (AGMs). Three wild-caught male AGMs and seven singly housed wild AGMs were euthanized on day 1 and day 45 post-capture, respectively, and compared with four wild males euthanized with a rifle as controls. Morphometric analyses of the adrenal cortices and the cortical zones were done using an image analyzer. By day 45, the confined animals were clinically healthy, but had lost 47% mean body weight despite ad libitum feeding. The width of zona fasciculata in the controls was significantly smaller compared with that of 45-day monkeys (P < 0.05). Numerous acidophilic, hyperplastic and hypertrophic cells were present in the zona fasciculata of the 1-day confined AGMs. In the 45-day monkeys, there was glandular hyperplasia in the zona glomerulosa and the acini were distended and vacuous; yellow, granular pigmentation was distributed in the zona fasciculata. Acute stomach lesions represented by petechiation were seen in one monkey on day 1. Deep, circular, mucosal erosions, one to five in number and measuring from 0.5 to 1 mm in diameter, were present in three monkeys on day 45 post-capture. There were no adrenal cortex or stomach lesions in the rifle-shot monkeys. In conclusion, pathological lesions in the adrenal glands, and stomachs of the wild AGMs and weight loss occurred within the initial 45-day period following capture and confinement.

Adrenal Glands↗

PubMed1994

Stimulus-driven attentional capture: evidence from equiluminant visual objects.

Previous work has shown that abrupt visual onsets capture attention. Possible attention. Possible mechanisms for this phenomenon include (a) a luminance-change detection system and (b) a mechanism that detects the appearance of new perceptual objects. Experiments 1 and 2 revealed that attention is captured in visual search by the appearance of a new perceptual object even when the object is equiluminant with its background and thus exhibits no luminance change when it appears. Experiment 3 showed that a highly salient luminance increment alone is not sufficient to capture attention. These findings suggest that attentional capture is mediated by a mechanism that detects the appearance of new perceptual objects.

Attention↗

PubMed1994

The structure of attentional control: contingent attentional capture by apparent motion, abrupt onset, and color.

Five spatial cuing experiments tested 2 hypotheses regarding attentional capture: (a) Attentional capture is contingent on endogenous attentional control settings, and (b) attentional control settings are limited to the distinction between dynamic and static discontinuities (C. L. Folk, R. W. Remington, & J. C. Johnston, 1992). In Experiments 1 and 2, apparent-motion precues produced significant costs in performance for targets signaled by motion but not for targets signaled by color or abrupt onset. Experiment 3 established that this pattern is not due to differences in the difficulty of target discrimination. Experiments 4 and 5 revealed asymmetric capture effects between abrupt onset and apparent motion related to stimulus salience. The results support the hypotheses of Folk et al. (1992) and suggest that stimulus salience may also play a role in attentional capture.

Adolescent↗

PubMed1994

Stimulus-driven capture and attentional set: selective search for color and visual abrupt onsets.

Recent evidence suggests that attentional capture is contingent on the attentional control setting induced by the task demands (C. L. Folk, R. Remington, & J. C. Johnston, 1992). Because the experiments on which these conclusions are based can be criticized for several reasons, the contingent capture hypothesis was tested using 2 visual search tasks in which subjects searched multielement displays in which a color singleton and onset singleton were simultaneously present. Both experiments show that the contingent capture hypothesis does not hold: Irrespective of attentional set, attention was captured by the most salient singleton. The findings suggest a stimulus-driven model of performance in which selection is basically determined by the properties of the featural singletons present in the visual field.

Adolescent↗

PubMed2000

Contingent capture for onsets and offsets: attentional set for perceptual transients.

Four experiments were conducted to examine whether attentional set affects the ability of visual transients (onsets and offsets) to capture attention. In the experiments, visual search for an identity-defined target was conducted. In the first 3 experiments, the target display either onset entirely or was revealed by offsetting camouflaging line segments to reveal letters. Prior to the target display, there was a noninformative cue, either an onset or an offset, at one of the potential target locations. Cues that shared the same transient feature as the target display captured attention. The lack of predictable target transients led to attentional capture by all forms of transients. The final experiments with luminance changes without offsets or onsets showed attentional capture when the luminance changes were large. The results suggest that attentional set can be broadly or narrowly tuned to detect changes in luminance.

Adolescent↗

PubMed2003

When something old becomes something new: spatiotemporal object continuity and attentional capture.

This article examines the possibility that the visal system treats dynamic cues a instances of new perceptual objects undersome circumstances. Using the contingent capture paradigm (C. L. Folk, R. W. Remington, & J. C. Johnston, 1992), the author compared luminance change cues of different magnitude for their ability to capture attention when participants were set for new objects. Wheras small luminance changes failed to produce attentional capture, large luminance changs indeed captured attention, suggesting that they were treated as compatible with the participants' attentional set for new objects. It is argued that sufficiently large luminance transients led to a disurption of spatiotemporal object continuity and precipitated the emergence of a new perceptual object.

Adolescent↗

PubMed2005

Attentional capture by an unannounced color singleton depends on expectation discrepancy.

Eight experiments examined the conditions under which a color singleton that is presented for the 1st time without prior announcement captures attention. The main hypothesis is that an unannounced singleton captures attention to the extent that it deviates from expectations. This hypothesis was tested within a visual-search paradigm in which set-size effects were used to infer attentional capture. The results showed that attentional capture by an unannounced color singleton was due to a mismatch with expectations concerning the color of the object and not due to its being a singleton. Thus, the results imply that theories of attention have to consider expectation discrepancy as a determinant of attention shifts.

Adult↗

PubMed2003

Chaos-assisted capture of irregular moons.

It has been thought that the capture of irregular moons--with non-circular orbits--by giant planets occurs by a process in which they are first temporarily trapped by gravity inside the planet's Hill sphere (the region where planetary gravity dominates over solar tides). The capture of the moons is then made permanent by dissipative energy loss (for example, gas drag) or planetary growth. But the observed distributions of orbital inclinations, which now include numerous newly discovered moons, cannot be explained using current models. Here we show that irregular satellites are captured in a thin spatial region where orbits are chaotic, and that the resulting orbit is either prograde or retrograde depending on the initial energy. Dissipation then switches these long-lived chaotic orbits into nearby regular (non-chaotic) zones from which escape is impossible. The chaotic layer therefore dictates the final inclinations of the captured moons. We confirm this with three-dimensional Monte Carlo simulations that include nebular drag, and find good agreement with the observed inclination distributions of irregular moons at Jupiter and Saturn. In particular, Saturn has more prograde irregular moons than Jupiter, which we can explain as a result of the chaotic prograde progenitors being more efficiently swept away from Jupiter by its galilean moons.

Journal Article↗

PubMed2004

Mercury's capture into the 3/2 spin-orbit resonance as a result of its chaotic dynamics.

Mercury is locked into a 3/2 spin-orbit resonance where it rotates three times on its axis for every two orbits around the sun. The stability of this equilibrium state is well established, but our understanding of how this state initially arose remains unsatisfactory. Unless one uses an unrealistic tidal model with constant torques (which cannot account for the observed damping of the libration of the planet) the computed probability of capture into 3/2 resonance is very low (about 7 per cent). This led to the proposal that core-mantle friction may have increased the capture probability, but such a process requires very specific values of the core viscosity. Here we show that the chaotic evolution of Mercury's orbit can drive its eccentricity beyond 0.325 during the planet's history, which very efficiently leads to its capture into the 3/2 resonance. In our numerical integrations of 1,000 orbits of Mercury over 4 Gyr, capture into the 3/2 spin-orbit resonant state was the most probable final outcome of the planet's evolution, occurring 55.4 per cent of the time.

Journal Article↗

PubMed1997

Direct on-filter immunoassay of some beta-lactam antibiotics for rapid analysis of drug captured from the workplace atmosphere.

A simple competitive enzyme-linked immunoassay for the antibiotic ceftazidime and structurally similar beta-lactam antibiotics has been developed which can be performed directly on the surface of a cellulose nitrate filter used to capture the airborne drug during workplace monitoring for health and safety purposes. Post sampling analysis is performed on the filter retained within the air sampler. It involves two steps; the first a 10 min incubation in which the captured drug is dissolved and competes with drug immobilised within a protein conjugate on the surface of the filter for an enzyme-labelled antibody reagent, and the second, following washing under vacuum in situ, a 5 min incubation of substrate solution when colour develops on the spot at the location of the immobilised drug-protein conjugate. The intensity of the spot can be assessed visually within the sampler to ascertain the presence or absence of captured drug, or quantitative results can be obtained using an optical scanner. The intensity of the spots in linear from 10 ng to 1 microgram (r2 = 0.9996, n = 3) and the limit of detection is 1.9 ng of captured drug (10 ng for visual discrimination between this standard and the zero). The assay is precise with between-assay RSD values of < 4% over the linear range of the assay.

Air Pollutants, Occupational↗

PubMed2004

Capturing of acidic macromolecules from biological samples using a temperature-responsive polymer modified with poly-l-lysine.

We synthesized a temperature-responsive polymer, N-(isopropylacrylamide)-methacrylic acid copolymer, to which poly-l-lysine was introduced. The synthesized polymer as well as the parent polymer showed reversible soluble-insoluble changes in response to temperature changes across the lower critical solution temperature at 32 degree C in an aqueous solution. We found that the polymer efficiently captured acidic bio-macromolecules such as RNA, glycosaminoglycans and mucin-type glycoproteins in biological samples, and the captured molecules were recovered using aqueous NaCl solutions at high concentration. The target acidic molecules thus obtained will be employed for further studies such as structural analysis after brief desalting procedure. The proposed method does not require any chromatographic separations, but only needs a small volume of an aqueous salt solution for releasing captured molecules. Overall procedures are quite easy and simple, and are completed at least within 1 h. We show a few examples for capturing RNA and glycosaminoglycans from cultured cells using the polymer.

Acrylamides↗

PubMed2003

Large-scale capture and partial purification of plasmid DNA using anion-exchange membrane capsules.

The demand for larger quantities of high-purity plasmids continues to increase. Substantial quantities of plasmid DNA are needed to support viral and plasmid-based gene-therapy programmes, including drug delivery, preclinical and clinical studies and production of DNA vaccines. Reliance on fermentation, which generates large lysate volumes, for producing the needed quantities of plasmid DNA is becoming more widespread. Development of an efficient capture-unit operation for very large plasmid DNA molecules from these large lysate volumes can present a considerable challenge. Use of conventional chromatographic beaded media for plasmid capture is problematic, owing to their restrictive pores, which limits access of the large DNA molecules to only those binding sites on the beads' outer surface. Anion-exchange membranes, on the other hand, have large convective pores that allow the plasmid DNA to readily access all of the membrane's anionic binding sites, even at very high flow rates. The convenience of anion-exchange membranes, configured in ready-to-use capsules, can greatly simplify large-scale plasmid purification strategies. The effectiveness of membrane-based technology for the capture of a pCAT reporter plasmid from large volumes of clarified Escherichia coli lysate was demonstrated. The captured and eluted plasmid pool was found to have been concentrated 10-fold with a reduction in endotoxin of four orders of magnitude.

Adsorption↗

PubMed2003

Bachmann's bundle versus right atrial appendage capture.

The tissue in the high intraatrial septum in the region of Bachmann's Bundle (BB) exhibits electrophysiological properties that differ from the right atrial appendage (RAA). As BB pacing emerges as an alternative to RAA pacing, the feasibility of using automatic capture recognition technology in this location should be examined. At implant, active-fixation leads were consecutively placed in the RAA, then the BB in 18 patients (55.5% men, mean age 77.1 +/- 9.1). There was no significant difference between BB and RAA in the average capture threshold (1.12 vs 1.77 V, P = 0.09), sensing threshold (3.85 vs 3.69 mV, P = 0.84), impedance (508 vs 529 Ohms, P = 0.64), evoked response (1.78 vs 1.67 mV, P = 0.83), and polarization (0.41 vs 0.46 mV, P = 0.84) between. The difference in tissue characteristics was not associated with a different evoked response measured by the ventricular capture recognition algorithm. Based on the analogous evoked response and polarization values, capture recognition technology designed for the atrium will most likely be applicable at both pacing sites.

Aged↗