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Repellency of live potted plants against Anopheles gambiae from human baits in semi-field experimental huts.

The repellency of potted plants against the malaria vector Anopheles gambiae sensu stricto Giles was quantified in experimental huts under semi-field conditions inside a screen-walled greenhouse. Ocimum americanum Linnaeus (Labiatae), Lantana camara L. (Verbenaceae), and Lippia uckambensis Spreng (Verbenaceae) repelled at an average of 39.7% (95% confidence interval [CI] = 29.6-48.4%), 32.4% (95% CI = 19.7-43.1%), and 33.3% (95% CI = 21.5-43.3%) of the mosquitoes, respectively (P < 0.0001 for all treatments). This was determined by logistic regression, allowing for variations associated with different bait hosts, sampling huts, and replicate test nights. In contrast, Ocimum kilimandscharicum Guerke (Labiatae), Ocimum suave Willd. (Labiatae), Corymbia citriodora Hook (Myrtaceae), Azadirachta indica A. Juss (Meliaceae), Tagetes minuta L. (Asteraceae), and Hyptis suaveolens Poit. (Lamiaceae) did not significantly repel mosquitoes. The combination of O. americanum with either L. camara or L. uckambensis repelled 31.6% (95% CI = 19.7-41.7%) and 45.2% (95% CI = 34.7-54.0%) of the mosquitoes, respectively (P < 0.0001 for both treatments). This study is the first to show that live intact plants can reduce domestic exposure to malaria vector mosquitoes. As such, they may represent a new, sustainable and readily applicable malaria vector control tool for incorporation into integrated vector management programs.

Animals↗

Impact of cattle keeping on human biting rate of anopheline mosquitoes and malaria transmission around Ziway, Ethiopia.

OBJECTIVE: To assess the impact of livestock keeping on the human biting rate (HBR) of anopheline mosquitoes and malaria transmission around Ziway in the middle course of the Ethiopian Rift Valley. DESIGN: As a passive experiment, man landing captures were done in homesteads with mixed dwelling, separate cattle shed and without livestock; and as an active experiment, captures were in experimental tukuls (huts) of cattle, goats, and without livestock. Parasite and spleen rates of children were compared among those residents under variable living conditions mentioned for passive experiment. SUBJECTS: For entomological study, human-baits were used for man-landing captures of mosquitoes. Study subjects for parasitological and clinical studies were children below 10 years old. MAIN OUTCOME MEASURES: Human-biting rate (HBR) of anopheline mosquitoes; and the parasite and spleen rates of the study subjects in different living conditions. RESULTS: In the passive experiment, the mean HBR of Anopheles arabiensis in mixed dwelling, separate cattle shed and without livestock was 8.45, 4.64 and 5.97, respectively. Similarly, the HBR of An. pharoensis was 2.88, 1.79 and 1.61, respectively. In the active experiment, the mean HBR of An. arabiensis in tukuls with cattle, goats, and without livestock was 3.50, 3.38 and 1.43 respectively; while that of An. pharoensis was 0.37, 0.70 and 0.55 respectively. Parasitologically, mean parasite rates of 26.67%, 15.05% and 23.85% were, respectively, recorded from children living under the above conditions stated for passive experiment. Similarly, the mean spleen rates of 50.0%, 26.9%, and 47.37% were recorded, respectively. CONCLUSION: These observations in the present study indicate that the presence of cattle in homesteads tends to increase the man biting rate of An. arabiensis, although differences in the mean HBR of vector mosquitoes were not statistically significant for all groups. In contrast, cattle keeping in separate cattle sheds outside of the human dwellings tends to reduce the man biting rate of An. arabiensis and malaria transmission in the study area.

Adult↗

Preliminary studies on the biting activity and transmission of Onchocerca volvulus by Simulium neavei (diptera: simuliidae) in Kashoya-Kitomi focus, Western Uganda.

OBJECTIVE: To determine the biting activity and onchocerciasis transmission in Kashoya-Kitomi focus, Western Uganda. DESIGN: Cross-sectional survey in randomly selected sites. SETTING: Three districts in Western Uganda. METHOD: Crab trapping and examination for immature stages of Simulium neavei and full day human bait catches and dissections were conducted at three sites. RESULTS: A total of 338 crabs were caught and out of this 372 (95.9%) were positive with immature stages of S. neavei with mean S. neavei/crab of 6.9. High biting density of S. neavei was observed in the two sites in Kabarole and Mbarara districts whereas an extremely low density was observed at a site in Bushenyi. Diurnal biting pattern revealed two peaks in the morning (09:00-10:00 hours.) and in the late afternoon (14:00-15:00 hours.) with a mid-day lull. The infection/infective rates were 53.3/11.0%, 30.7% to 7.8% and 20.0/0% for sites in Mbarara, Kabarole and Bushenyi districts, respectively. Monthly biting rates/ Transmission potentials for Mbarara, Kabarole and Bushenyi districts were 6231/5437, 9244/2916 and 247/0. CONCLUSION: There was high transmission of onchocerciasis observed in Kashoya-Kitomi focus despite the ongoing ivermectin treatment. The need for improved chemotherapeutic coverage and instituting other control measures may be necessary.

Animals↗

Sampling techniques for adult Afrotropical malaria vectors and their reliability in the estimation of entomological inoculation rate.

Various entomological indicators and sampling techniques are used to monitor and evaluate the impact of many vector control interventions. A number of methods have been used in sampling mosquitoes for the purpose of estimating the entomological inoculation rate (EIR) and each is subject to some bias or shortcomings. It was the aim of this paper to critically evaluate the most common mosquito sampling techniques in relation to their reliability in the estimation of EIR. The techniques include man-landing, light trap, light trap/bednet combination and odour-baited traps. Although man-landing technique is the most reliable, it however, expose the catcher to mosquito-borne infections. On the other hand, light traps have been found to capture mosquitoes with higher sporozoite rates as compared to those from human bait catch thus leading to an overestimation of EIR. From an epidemiological point of view, the use of light-trap-bed net combination is an approach that is more meaningful than using light trap alone because, a light trap functions more efficiently when placed near the normal flight paths of mosquitoes such as inside huts or under the eaves. Unfortunately, it has been shown that estimates of EIR are influenced by trap position, hence affecting the number caught and the sporozoite rates. A variety of bednets have been used to sample mosquitoes attracted to man. Studies have shown that bednet traps normally catch fewer mosquitoes than do human baits outside them. Although the collections by indoor resting technique give a good estimate of the mean house density in a given area, they may not necessarily give a good estimate of EIR. Thus the development of improved sampling systems based on an improved understanding of host-oriented behaviour is needed. Moreover, there is need to standardise all the sampling techniques in use to enable us make valid comparisons between various studies done by different people and in different areas. In this article, the inherent limitations of conventional mosquito sampling techniques when used in estimating the EIR are discussed.

Africa↗

An outbreak of Plasmodium vivax malaria in Far North Queensland, 2002.

OBJECTIVE: To describe an outbreak of Plasmodium vivax malaria in Far North Queensland in 2002. DESIGN: Epidemiological and entomological investigations; molecular analyses of the infecting parasites. MAIN OUTCOME MEASURES: Case characteristics, adult and larval mosquito counts at the outbreak location, haplotyping of parasites in blood samples from different cases determined through sequencing of AMA1 and MSP1 genes. RESULTS: A man with imported P. vivax malaria stayed at a camping ground 95 km north of Cairns in late September 2002. This led to an outbreak of P. vivax malaria in 10 adults who stayed at the camping ground in October. Large numbers of Anopheles farauti sensu lato larvae were present in stagnant pools in a creek at the camping ground, and many adult mosquitoes were collected nearby. Not only had most of the infected patients been exposed to mosquitoes at night, they were also less likely than other campers to have used insect repellents appropriately (odds ratio, 0.01; P < 0.001). Two different haplotypes of P. vivax, only one of which was detected in the imported case, were involved in the outbreak. CONCLUSIONS: Although local transmission of malaria is rare in Far North Queensland, the risk is probably higher in the dry season (September to December). Campers need to be aware of the increased risk of mosquito-borne diseases. Sexual recombination of multiple gametocytes in mosquitoes infected by the imported case may have resulted in the two haplotypes of P. vivax involved in the outbreak.

Adult↗

Field-user acceptability of new camouflage face paint formulations in the Republic of Korea.

New formulations of camouflage face paint (CFP), one with 30% N,N-diethyl-3-methylbenzamide (DEET) and the other without DEET, were evaluated for soldier-user acceptability during a military field-training exercise in the Republic of Korea. Soldiers testing the CFP formulations were members of one of four U.S. Army infantry companies (A, B, C, or D). The formulations were evaluated while soldiers participated in simulated combat exercises for 5 days during hot, humid summer weather in Korea. Results showed that soldiers found both of the new formulations easier to apply (91.3% of respondents who used CFP without DEET and 87.9% of respondents who used CFP with DEET) and remove (82.6% without DEET and 81.2% with DEET) than the previous standard military-issue CFP. Soldier acceptability was higher for the new CFP formulation with 30% DEET (70.5%) than for the formulation without 30% DEET (52.9%). Soldiers recommended it more frequently (70.5%) than the formulation without 30% DEET (50.0%). The new CFP formulation with 30% DEET was rated more often (79.5%) as either good or excellent than the new formulation without 30% DEET (67.4%). Soldiers reported that the CFP formulation with 30% DEET more successfully camouflaged the face (92.7%) than the formulation without 30% DEET (80.0%).

Adolescent↗

Survey of repellent use by service members arriving in Kuwait for Operation Iraqi Freedom 2.

The 8th Medical Brigade, deployed to Kuwait in January 2004 in support of Operation Iraqi Freedom provided theater-wide health service support and medical command and control. An historic "surge" of troops and equipment occurred during our deployment as 140,000 service members (SMs) redeployed out of theater and 110,000 arrived to take their place. The brigade's preventive medicine personnel conducted a questionnaire-based survey at military camps in northwestern Kuwait in mid-March to determine whether SMs had been provided with supplies and information to protect them against endemic vector-borne diseases. Of 1,200 questionnaires distributed, 870 (73%) were returned. In addition to military affiliation information, the survey contained specific questions related to the provision of n-diethyl-m-toluamide repellents, permethrin and treated uniforms, and medical threat briefings. On average, less than one-half of the SMs received these resources before their arrival in Kuwait. Significant differences of resource provision existed among service branches, components, and mobilization or deployment stations.

Humans↗

Mosquitoes and mosquito repellents: a clinician's guide.

This paper is intended to provide the clinician with the detailed and scientific information needed to advise patients who seek safe and effective ways of preventing mosquito bites. For this review, clinical and analytical data were selected from peer-reviewed research studies and review articles, case reports, entomology texts and journals, and government and industry publications. Relevant information was identified through a search of the MEDLINE database, the World Wide Web, the Mosquito-L electronic mailing list, and the Extension Toxicology Network database; selected U.S. Army, U.S. Environmental Protection Agency, and U.S. Department of Agriculture publications were also reviewed. N,N-diethyl-3-methylbenzamide (DEET) is the most effective, and best studied, insect repellent currently on the market. This substance has a remarkable safety profile after 40 years of worldwide use, but toxic reactions can occur (usually when the product is misused). When DEET-based repellents are applied in combination with permethrin-treated clothing, protection against bites of nearly 100% can be achieved. Plant-based repellents are generally less effective than DEET-based products. Ultrasonic devices, outdoor bug "zappers," and bat houses are not effective against mosquitoes. Highly sensitive persons may want to take oral antihistamines to minimize cutaneous reactions to mosquito bites.

Animals↗

Bartonella spp. in deer keds, Lipoptena mazamae (Diptera: Hippoboscidae), from Georgia and South Carolina, USA.

Deer keds, Lipoptena mazamae (Diptera: Hippoboscidae), were collected from white-tailed deer (Odocoileus virginianus) and humans in Georgia and South Carolina, USA (1 October 2001-6 January 2005) and screened for the presence of DNA from Bartonella spp. Forty deer keds were screened for Bartonella spp. by polymerase chain reaction using primers specific to the riboflavin synthase gene (ribC) of Bartonella. Bartonella species closely related to Bartonella schoenbuchensis and to the etiologic agent of cat-scratch disease (Bartonella henselae) were detected in 10 keds and one ked, respectively.

Animals↗

[A comparative analysis of the potential epidemiological importance of mosquitoes in the genus Anopheles under the conditions of the anthropogenic landscapes in the Chu Valley, Kyrgyz Republic. 1. The activity of the attack of malarial mosquitoes on man].

Six species of the genus Anopheles (An. claviger were prevalent, An. messeae and An. hyrcanus were intermediate, and An. martinius, An. algeriensis and An. superpictus were rare) were recorded in the Chu [correction of Chuisk] Valley in the north of the Republic of Kirghizia. Due to the fact that the malaria situation was worsening, the potential epidemiological significance of Anopheles that predominate under the man-made districts of the region under study was comparatively analyzed. Mosquitoes were collected in the 1991-1992 seasons. This paper gives data on the seasonal attacking activity of the predominant Anopheles species in the foothills and lowlands of the Chu River valley. The maximum seasonal attacking activity of mosquitoes has been found to coincide with the time of their greatest numbers and to be in the hot summer period (July-August) that makes it favourable for the causative agents of malaria to develop.

Animals↗

Possibilities of long-term protection against blood-sucking insects and ticks.

Insecticidal and repellent properties and residual action of permethrin and deltamethrin and two commercial preparations Peripel 55 and Permanone were investigated under laboratory and field conditions. The tests were conducted on ticks, mosquitoes and houseflies. Permethrin in concentrations of 0.036-2.276 mg.m-2 caused 90-100% mortality and deltamethrin in concentrations of 0.012-0.75 mg.m-2 induced 78-100% mortality in ticks after 24 hrs exposure (Table 1). Good repellent properties of the preparation Peripel 55 were confirmed in experiments on mosquitoes Aedes aegypti (Table 2 and Fig. 1). Experiments confirmed good residual effects (Fig. 3 and 4) of the preparations tested. Peripel 55 retained 100% effectiveness up to 4 months and following two washings. The preparation Permanone exhibited 100% effectiveness for 1 month and a decrease in effectiveness to 52% after the first washing. In the period from June to September, the repellent properties of Permanone were observed in a group of 17 forestry women workers in a field trial. The index of repellency ranged from 57 to 80 (average 71) in June and July and from 40 to 100 (average 74) in August and September. The difference in the number of ticks that clung to the workers in the experimental group who had their clothes treated with the preparation tested was significant in comparison with the control group at p < 0.1, 0.05 and 0.01.

Aedes↗

Lyme disease: recognition, management, and prevention in the primary care setting.

UNLABELLED: This activity is designed for practitioners who see patients with tick bites, Lyme disease, or suspected Lyme disease in their practice, whether or not the practitioner is in an endemic area for Lyme disease. GOAL: To help primary care practitioners recognize and treat Lyme disease and provide preventive counseling. OBJECTIVES: 1. Be familiar with the terminology used for the causative agent of Lyme disease, its tick vector and reservoirs in nature, and where the disease is endemic. 2. Know the features of the common, characteristic clinical forms of Lyme disease. 3. Appreciate the uses and limitations of laboratory testing for this infection. 4. Understand early antibiotic treatment of Lyme disease, the management of a tick bite, and preventive measures.

Anti-Bacterial Agents↗

Malaria transmission in a region of savanna-forest mosaic, Haut-Ogooué, Gabon.

During the 2 years 1993 to 1995, an entomological survey was carried out in the savanna-forest area of Franceville, Gabon, investigating malaria transmission in one suburban district of Franceville (Akou) and in one rural village (Benguia). The biting rates of the Anopheles vectors were 10 times higher in the rural zone compared to the suburban zone. Anopheles funestus Giles was the predominant species in both zones followed by Anopheles gambiae s.l. Giles. The densities of Anopheles nili Theobald and Anopheles moucheti Evans were very low. In the suburban zone, transmission was maintained throughout the year by An. funestus and An. gambiae s.l., whereas in rural zones the secondary vectors An. nili and An. moucheti were also involved in transmission. Humans in a suburban setting received one infective bite per person every 4 days, whereas in the rural area the infective biting rate was 4 times higher. Considering each vector, the observed entomological inoculation rates (EIRs) were one infective bite per person every 6 and 17 days for An. funestus and An. gambiae s.l., respectively, at Akou. At Benguia, the EIRs were one infective bite per person every 2, 3, 6, and 19 days for the 4 An. funestus, An. gambiae s.l., An. nili, and An. moucheti, respectively. The predominance of An. funestus over An. gambiae s.l. and its high EIR make it the most important malaria vector in this region of Haut-Ogooué.

Aedes↗

[Anopheles mascarensis (De Meillon, 1947): main vector of malaria in the region of Fort-Dauphin (south-east of Madagascar)].

Anopheles funestus and Anopheles gambiae s.l. have been considered until now the major vectors of malaria everywhere in Madagascar. Anopheles mascarensis, a mosquito native to Madagascar, has been identified in Sainte-Marie island as a secondary vector only. In 1997, an entomological study was carried out to identify the malaria vectors in the area of Fort-Dauphin, South-East of Madagascar. Every month, mosquitoes were collected from landing catches on human volunteers (from 7:00 am to 5:00 pm inside dwellings and from 7:00 am to 0:00 pm outside) and from knockdown spray-collections indoors. An mascarensis was the most abundant mosquito, the average number of An. mascarensis bites per man/night was 7.6. The sporozoite index was 0.89%. Despite the presence of An. funestus and An. gambiae s.l., An. mascarensis was found to be responsible for 2/3 of the infectious bites (25 infectious bites per man/year). An. mascarensis is widely distributed ih Madagascar but only specimens from the east coast have been found to carry sporozoites of human malaria. Further arguments are thus advanced for the hypothesis according to which a sibling species of An. mascarensis is present in Madagascar.

Animals↗

[Impact of the use of permethrin pre-impregnated mosquito nets on malaria transmission in a hyperendemic village of Senegal].

The efficacy of permethrin-treated bednets was evaluated in Wassadou, a hyperendemic village located in the Sudanese grasslands of southeast Senegal. Pretreatment data were collected between 1992 and 1993. Bednets were distributed to the whole population in June 1995 and impact of their use on vector populations and malaria transmission was evaluated until November 1995. This period corresponds to the rainy season during which malaria transmission is highest. Data were compared with a control village in which bednets were not distributed. Findings showed that use of bednets led to a sharp decrease in the density of the vector population and malaria transmission. The number of bites by Anopheles gambiae s.1. decreased 69%. The density of blood-laden and pregnant females inside dwellings decreased 91% and 96% respectively. The sporozoite index of females captured on the skin decreased 76% and the daily rate of entomological inoculation decreased 88%. This impact was not great enough to eliminate the risk of infection. Prolonged study over a period of 4 to 5 years is needed to evaluate the impact of long-term use of insecticide-treated bednets on vector population and malaria transmission.

Animals↗

Studies on bionomics of Anopheles fluviatilis and its sibling species in Nainital district, U.P.

A study on the bionomics of Anopheles fluviatilis sensu lato was carried out in two physiographic regions, viz. Bhabar and Terai of District Nainital, Uttar Pradesh. In both areas, An. fluviatilis was found resting indoors predominantly in cattlesheds. Cytological examination of An. fluviatilis revealed that species T and U were sympatric in Bhabar and Terai villages with predominance of species T. These two sibling species appear to be poor vectors of malaria.

Animals↗