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PubMed2004

Qualitative human chorionicgonadotropin testing of cervicovaginal washings for the detection of preterm premature rupture of membranes.

OBJECTIVE: This study was performed to determine whether a qualitative human chorionicgonadotropin (hCG) test using cervicovaginal washings obtained from pregnant women is a useful predictor of recent preterm premature rupture of the membranes (PPROM). STUDY DESIGN: A prospective observational analysis of qualitative hCG testing on cervicovaginal washings in patients with either known PPROM or with intact membranes was performed. Patients with singleton gestations between 24 and 34 completed weeks were included. PPROM patients had documented confirmatory tests, including visualized pooling of amniotic fluid in the vaginal vault that was nitrazine-positive and demonstrated ferning. Those with vaginal bleeding or an anomalous fetus were excluded. Before collection, all patients were screened for bacterial vaginosis and trichomoniasis. Washings were then collected from the posterior vaginal fornix with the use of a 5-mL sterile saline irrigation and aspiration technique. Samples were then agitated manually and applied to the Quickvue Onestep qualitative pregnancy test (Quidell, San Diego, Calif) with a threshold of 25 mIU/mL. Data were analyzed with the t test, chi 2, and Fischer exact tests. Sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values were calculated. When applicable, all 2-tailed P <.05 were considered significant. RESULTS: Fifty-two patients were enrolled in the study with 24 in the PPROM group and 28 in the control group. The 2 groups were similar with respect to demographic characteristics and wet preparation results. The mean gestation age was 31.4 +/- 2.4 (X +/- SD) and 30.8 +/- 2.7 weeks in the PPROM and control groups, respectively. The hCG test was positive in 19 (79%) of the PPROM patients and in 1 (3.6%) of the controls (sensitivity 79%, specificity 96%, PPV 95%, NPV 84%). CONCLUSION: Qualitative hCG testing of cervicovaginal washings appears to be an useful predictor of PPROM.

Adult↗

PubMed2005

Soil remediation: humic acids as natural surfactants in the washings of highly contaminated soils.

The remediation of the highly contaminated site around the former chemical plant of ACNA (near Savona) in Northern Italy is a top priority in Italy. The aim of the present work was to contribute in finding innovative and environmental-friendly technology to remediate soils from the ACNA contaminated site. Two soils sampled from the ACNA site (A and B), differing in texture and amount and type of organic contaminants, were subjected to soil washings by comparing the removal efficiency of water, two synthetic surfactants, sodium dodecylsulphate (SDS) and Triton X-100 (TX100), and a solution of a natural surfactant, a humic acid (HA) at its critical micelle concentration (CMC). The extraction of pollutants by sonication and soxhlet was conducted before and after the soil washings. Soil A was richer in polycyclic aromatic hydrocarbons, whereas soil B had a larger content of thiophenes. Sonication resulted more analytically efficient in the fine-textured soil B. The coarse-textured soil A was extracted with a general equal efficiency also by soxhlet. Clean-up by water was unable to exhaustively remove contaminants from the two soils, whereas all the organic surfactants revealed very similar efficiencies (up to 90%) in the removal of the contaminants from the soils. Hence, the use of solutions of natural HAs appears as a better choice for soil washings of highly polluted soils due to their additional capacity to promote microbial activity, in contrast to synthetic surfactants, for a further natural attenuation in washed soils.

Chemical Industry↗

PubMed2006

Exposure data for personal care products: hairspray, spray perfume, liquid foundation, shampoo, body wash, and solid antiperspirant.

Reliable exposure information for cosmetic and other personal care products and ingredients is needed in order to conduct safety assessments. Essential information includes both the amount of product applied, and the frequency of use. To obtain current data, studies to assess consumer use practices were undertaken. Six widely used personal care product types were included in the studies. Five of the products were cosmetics (spray perfume, hairspray, liquid foundation, shampoo, body wash) and one product was a cosmetic/over-the-counter drug product (solid antiperspirant). Three hundred and sixty women, ages 19-65 years, who regularly use the products of interest, were recruited at 10 different geographical locations within the US. The number of recruits was chosen to ensure a minimum of three hundred completed responses per product type. Subjects were provided with a new container of the brand of product they normally use and kept diaries and recorded detailed daily usage information over a two week period. Products were weighed at the start and completion of the study in order to determine the total amount of product used. Statistical analyses of the data were conducted to derive summary distributions of use patterns. The geometric mean and median usage per application, respectively, for the six product types were: spray perfume, 0.33 g and 0.23 g; hairspray, 2.58 g and 1.83 g (aerosol); 3.64 g and 2.66 g (pump); liquid foundation, 0.54 g and 0.36 g; shampoo, 11.76 g and 9.56 g; body wash, 11.3g and 9.5 g; and solid antiperspirant, 0.61 g and 0.45 g. The mean and median usage per day for the six product types were: spray perfume, 0.53 g and 0.34 g; hairspray, 3.57 g and 2.71 g (aerosol); 5.18 g and 3.74 g (pump); liquid foundation, 0.67 g and 0.45 g; shampoo, 12.80 g and 10.75 g; body wash, 14.5 g and 12.9 g; and solid antiperspirant, 0.79 g and 0.59 g. The mean number of applications per day for spray perfume, hairspray, liquid foundation, shampoo, body wash, and solid antiperspirant was 1.67, 1.49 (aerosol) and 1.51 (pump), 1.24, 1.11, 1.37, and 1.3, respectively. This study provides current exposure information for commonly used products which will be useful for risk assessment purposes.

Administration, Topical↗

PubMed2005

Washing enhanced electrokinetic remediation for removal cadmium from real contaminated soil.

The main objective of this study is to evaluate the combination of electrokinetic remediation and soil washing technology in order to remove cadmium from contaminated soil. This paper presents the results of an experimental research undertaken to evaluate different washing and purging solutions to enhance the removal of cadmium from a real contaminated soil during electrokinetic remediation. Two different experimental modules were applied in the laboratory. Soil was saturated with tap water, while acetic and hydrochloric acids, as well as ethylenediaminetetraacetic acid (EDTA) were used as purging solutions in the first module. Results show that there was a decrease of cadmium concentration near anode, but a significant increase in the middle of the cell, due to the increasing pH. Citric, nitric and acetic acids were used for soil washing and purging solutions in the second module. In this case, an 85% reduction of cadmium concentration was achieved. Therefore, results indicate that soil pH and washing solutions are the most important factors in governing the dissolution and/or desorption of Cd in a soil system under electrical fields.

Acids↗

PubMed2005

Assessment of pilot-scale acid washing of soil contaminated with As, Zn and Ni using the BCR three-step sequential extraction.

This study performed pilot-scale washing of soil contaminated with both oxyanion and cations as a recalcitrant remediation case due to their different chemical behavior. The soil contaminated with As, Zn and Ni, partially recalcitrant due to their strong binding properties, was obtained near a closed iron/serpentine mining area. This study monitored the variation of chemical speciation of As, Zn and Ni for acid solutions and particle size fraction using the BCR sequential extraction and evaluated the optimal condition of physical separation of highly contaminated fine particles for enhanced washing. H(2)SO(4) and H(3)PO(4), including competitive oxyanions, enhanced removal of As with the simultaneous extraction of Zn and Ni. Less nickel from the residual fraction in coarse particles was extracted than As and Zn due to the recalcitrant serpentine. Fe/Mn oxide, organic/sulfides and residual fractions in fine particles were enriched with contaminants due to the high surface areas and recalcitrant minerals. The chemical extraction of As was also restricted in the fine particles, whereas the chemical extraction of Zn and Ni was determined by the residual form of various particle size fractions. Further extraction was limited in the exchangeable and residual fractions and retained a gradual extraction from Fe/Mn oxide and organic/sulfides fractions, which indicated an instant detachment from the easily bound fraction. Correspondingly, extraction from the acid-attackable fraction was related to the exchangeable Fe/Mn oxide and organic/sulfides fractions. Due to the limitation of chemical extraction, the physical separation of fine particles could enhance the effectiveness of acid washing. In addition, the chemical properties of the soil were affected by strong acid washing. The treated soil then needed to be regenerated.

Acids↗

PubMed2006

Long-term wash-resistant effects of brief interaction of xanomeline at the M1 muscarinic receptor.

Compared to other M(1) muscarinic acetylcholine receptor (M(1) mAChR) agonists, xanomeline demonstrates both reversible and persistent modes of binding to the receptor. In our study, we investigated the long-term consequences of brief incubation of Chinese hamster ovary cells expressing M(1) mAChR (M(1)-CHO) with low concentrations of xanomeline followed by washing off the free drug. Thus, M(1)-CHO cells were exposed to 100 nM xanomeline for 1h then washed extensively. Washed cells were either used immediately for binding assays or incubated for 23 h in the absence of free xanomeline. Only the latter treatment conditions resulted in marked attenuation of binding of the muscarinic radioligand [(3)H]N-methylscopolamine ([(3)H]NMS) to intact cells. Shortening the xanomeline pretreatment period to 1 min had the same trends as the 1h pretreatment, implying that xanomeline binds instantly to the receptor to elicit long-term wash-resistant effects. Presence of atropine during the brief period of xanomeline pretreatment did not markedly modulate xanomeline's long-term effects, which suggests that persistent anchoring of the xanomeline molecule to the M(1) receptor takes place at a site distinct from the orthosteric binding domain. Our findings suggest the possibility of a time-dependent transition of the conformation of the muscarinic M(1) receptor-xanomeline complex between states that vary in their ability to bind [(3)H]NMS. However, possible involvement of other mechanisms of long-term receptor regulation cannot be discounted.

Animals↗

PubMed2005

[Platelet-washing solution optimization].

Different washing and homogénéisation solutions are hereby analysed by comparing the evolution of functional indicators during the preservation of washed aphaeresis platelet concentrates: physiological pH 4.5 and 6 solutions, buffered physiological pH 6.8 glucose solution, and two physiological pH 7 citrate solutions with acetate. Prior acidification of platelet concentrates proved to be essential. Two washings with manual or automated technique, guarantee residual proteins at a level of less than 0.5 g. Solutions T-Sol Baxter or SSP Macopharma allow us to obtain a product that meet the PSL specifications. Routine since June 2004, washings are done with a physiological pH 6 solution, then homogeneised with T-Sol solution. Platelet recovery, swirling phenomenon, lack of agrgegates, pH maintenance, low increase in the platelet average volume and maintenance of intra-cell potassium level, suggest that platelet entirety is preserved beyond the product's expiration date. The platelet transfusion yield of these products is satisfactory.

Adult↗

PubMed2006

Examination of laparoscopic retrieval bag washings for malignant cells after hand-assisted laparoscopic radical nephrectomy and intact specimen removal.

OBJECTIVES: Port site metastases after hand-assisted laparoscopic radical nephrectomy have been described in the literature. There is no uniform agreement among urologists regarding the use of a retrieval bag before intact specimen removal. The aim of this study was to determine whether LapSac renal extraction bag washings contain malignant cells. METHODS: We prospectively obtained washings from the LapSac retrieval bag after hand-assisted laparoscopic radical nephrectomy and intact specimen removal for renal cell carcinoma. In 30 consecutive cases, after removal of the kidney specimen from the LapSac, the LapSac was irrigated with 50 mL sterile Hank's balanced salt solution. These washings were sent for cytologic examination. Cytologic evaluation was performed with a Thin Prep and the Papanicolaou method. RESULTS: We performed 30 hand-assisted laparoscopic radical nephrectomies for suspected renal cell carcinoma with the above protocol. One specimen was benign and one showed transitional cell carcinoma; these were excluded from the study. Six specimens were stage T1a, 17 were T1b, 1 was T2, 2 were T3a, and 2 were T3b. Histopathology revealed 27 specimens with clear cell renal cell carcinomas with Fuhrman grades from 1 to 4; 1 specimen showed chromophobe renal cell carcinoma. Margins were negative in all cases, and there were no gross or microscopic tumor violations. The cytologic results from 27 cases were negative and in 1 case with T3b renal cell carcinoma the LapSac washings were positive for malignant cells. CONCLUSIONS: The preliminary findings from our study show that low-stage, low-grade tumors removed laparoscopically with minimal manipulation do not exfoliate cells into their LapSac retrieval bags.

Carcinoma, Renal Cell↗

PubMed2005

Optimizing the APC residue washing process to minimize the release of chloride and heavy metals.

An optimization of the air pollution control (APC) residue washing process was carried out to minimize the release of chloride and heavy metals. Taking into account economic parameters such as the consumption of water and reaction time, the best relation found was a S/L ratio of 1/3 during 1 h. At a laboratory scale and according to the values obtained for chloride and heavy metals, the APC residue is classified as non-special according to Catalonian Regulations (Spain). Moreover, the pH of the solution, when MgSO(4) is added during the washing process, may be controlled by the formation of gypsum. In these conditions, the concentration of heavy metals will decrease as a consequence of the formation of their respective insoluble hydroxides. Therefore, the counter-current batch washing process with the addition of small amounts of MgSO(4) is revealed as an economically feasible treatment of the APC residue. This washed residue is ready to be used as secondary material or to be landfilled safely.

Air Pollutants↗

PubMed1998

The relevance of associative learning pathways in the development of obsessive-compulsive washing.

The relevance of associative learning in the development of Obsessive-Compulsive Disorder (OCD) was investigated in a group of 23 OCD patients whose main concern was washing and 23 age and sex matched control subjects who did not have OCD. OC washers completed an origins instrument based on Menzies and Clarke's (1993, Behaviour Research and Therapy, 31, 355-365) Origins Questionnaire (OQ) for the phobic disorders. Control subjects completed a modified version of this measure designed to give a comprehensive picture of their experiences with relevant contamination-related stimuli. In general, the results question the relevance of associative-learning per se in the development of OC washing. Direct and indirect conditioning events were very rare in the OCD group, accounting for less than 13% of cases. No significant differences between groups were found in the proportion of subjects who knew other OC washers, or had experienced direct associative-learning events prior to onset. Contrary to expectation, significantly more non-OCD subjects had experienced vicarious learning events related to dirt and washing than OCD subjects. However, of note, associative-learning events that took place during episodes of depression were significantly more frequently reported in the OCD group than in the control group. Depression appeared to play a facilitating role in the associative-learning of OC washing. The implications of these findings for theoretical accounts of OCD are discussed.

Adolescent↗

PubMed1976

Effect of different lysing and washing methods on free amino acid concentrations of sheep whole blood and erythrocytes.

1. A study was conducted to determine the effect of different washing and lysing methods on the free amino acid concentrations of sheep erythryocytes (RBC) and whole blood. Methods of lysis included laking, freeze-thawing or sonication. RBC were washed with isotonic solutions of either saline or sucrose. 2. Washing the RBC with isotonic sucrose resulted in significantly greater concentrations for ten of the amino acids. Greater amino acid concentrations were realized when washed RBC were sonicated and when the RBC of whole blood were lysed by laking. 3. Suggestions are made for future application to whole blood and RBC amino acid determinations.

Amino Acids↗

PubMed2002

Comparison of polymerase chain reaction-dependent methods for determining the presence of human immunodeficiency virus and hepatitis C virus in washed sperm.

OBJECTIVE(S): To evaluate the effectiveness of sperm washes by double capacitation in patients with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) and to compare between polymerase chain reaction (PCR)-dependent methods to determine viral presence after the procedure. DESIGN: Controlled prospective clinical trial. SETTING: Private and university-affiliated setting. PATIENT(S): Thirty-four HIV-positive men attending our center for assisted reproduction with their partners. INTERVENTION(S): Forty-one semen samples from seropositive males were obtained and washed through density gradients and swim-up and analyzed for the presence of HIV and HCV with either nested or one-round PCR. Qualitative and quantitative detection of DNA and RNA of HIV and RNA of HCV. RESULT(S): Of all the semen samples that were analyzed after washing, seven out of 41 samples tested positive for one or both viruses by nested PCR. The confirmation of these results with the currently used commercial methods of quantitative PCR (one-round PCR) resulted in all negatives. CONCLUSION(S): Sperm wash with nested PCR is the appropriate method to use in the assisted reproduction techniques that are offered to serodiscordant couples. The detection limits exhibited by one-round PCR do not offer a sufficient guarantee that transmission of all viral particles will be avoided since both viruses can be present in amounts lower than the detection threshold of this technique.

Adult↗

PubMed2000

Development and evaluation of a micropipette tip washing system.

A tip washing system has been developed for use with disposable polypropylene micropipette tips of different sizes. The primary hardware components of the system are constructed from polyvinyl chloride (PVC) and Plexiglas. The system relies on a low concentration of detergent, gravity or aspirator-assisted feed and flush of minimal volumes of distilled/deionized water, and air or oven drying of the tips. Comparative analyses of new versus washed tips in FAO/IAEA radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA) protocols indicated that disposable polypropylene micropipette tips of various sizes (e.g. 5-300 and 50-1000 microl) can be washed and reused at least ten times without introducing detectable variability into the results of either assay, if the tips are properly washed and dried using this system. Recovery cost of the system can be achieved after approximately eight reuses of 1000 tips, excluding technician time.

Enzyme-Linked Immunosorbent Assay↗

PubMed1996

Malignant cytological washings from radical prostatectomy specimens: a possible mechanism for local recurrence of prostate cancer following surgical treatment of organ confined disease.

PURPOSE: Local recurrence of prostate cancer following complete and successful resection of organ confined disease has been variably reported in men. We hypothesized that observed secretions from the cut distal urethra during radical prostatectomy may contain malignant prostatic epithelial cells and contribute to this problem. MATERIALS AND METHODS: A prospective study was done of prostate cytology specimens from 50 consecutive men with clinically organ confined adenocarcinoma of the prostate undergoing radical retropubic or radical perineal prostatectomy. Direct cytological evaluation by 1 examiner was used to identify malignant or benign cells in these washings. RESULTS: Of 33 radical perineal and 17 radical retropubic prostatectomy specimens organ confinement was confirmed in 58%. Malignant prostatic epithelial cells were observed in 24% of all cytology specimens. Of cytological washings from prostates with pathologically confirmed organ confined cancers 17% showed malignant cells. While perineural invasion was noted in a majority of tumors with positive washings, only Gleason grade was a statistically significant predictor of recurrence (p = 0.009). Surgical approach did not alter the rate of positive cytology. CONCLUSIONS: Malignant prostatic epithelial cells can be identified in the prostatic washings from men with pathologically organ confined prostate cancer. Surgical approach did not change the cytological findings. Gleason grade is a statistically significant predictor of cytological malignancy. These cells may represent a mechanism of failure following successful radical prostatectomy.

Adenocarcinoma↗

PubMed1994

Inflammatory mediator profile in urine and bladder wash fluid of patients with interstitial cystitis.

Interstitial cystitis is a syndrome of urinary urgency, frequency and suprapubic pain. We investigated the role of inflammatory mediators in 96 patients with histories and symptoms consistent with interstitial cystitis, and 13 controls from The New York Hospital-Cornell Medical Center, University of Washington and University of California at San Diego. Patients were classified into either group A (meets all criteria of the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases for inclusion in research studies), group B (meets all of these criteria but without glomerulations) or an "other" group. A small number of group A patients had detectable interleukin-6 in the urine. Urinary concentrations of tumor necrosis factor, prostaglandins E2, D2 and F2 alpha, and thromboxane B2 were not different among either patient groups or controls. Urine specimens contained inhibitors of the bioactivity of interleukin-6 and tumor necrosis factors but no differences between patients or controls were found. No factors chemotactic for human neutrophils were detected in a small patient sample. Bladder wash fluid concentrations of prostaglandins E2, D2 and F2 alpha, and thromboxane were much lower than urinary levels. Bladder wash fluid interleukin-6 and tumor necrosis factor were not detectable. The results suggest that while a small subset of patients may have elevated levels of interleukin-6 the majority of patients do not appear to have elevated levels of inflammatory mediators in the urine or bladder wash fluid. Evaluation of patient bladder tissue may indicate changes not detectable in urine or bladder wash fluid. Alternatively, other etiologies must be considered in those patients.

Adult↗

PubMed1985

Monoclonal antibodies against transitional cell carcinoma for detection of malignant urothelial cells in bladder washing.

The diagnosis of transitional cell carcinoma by cytological examination of exfoliated urinary cells is important in the early detection and followup of patients with this disease. Proper interpretation requires a skilled pathologist. Accuracy also is influenced by collection methods and nonmalignant pathological conditions of the bladder. An immunocytochemical technique using monoclonal antibodies G4 and E7 successfully identified tumor-associated antigens on the surface of transitional carcinoma cells obtained by bladder washings. The method, which uses immunoperoxidase staining, was compared to conventional Papanicolaou staining of bladder washings from 75 patients with and without transitional cell carcinoma. Patients were divided into 4 groups: group 1 (nontumor control)--15 patients with no pathological condition of the bladder or nonmalignant urological diseases, group 2 (nontransitional cell carcinoma)--19 patients with other urological malignancies, group 3-18 patients with active transitional cell carcinoma and group 4-23 patients with a history of transitional cell carcinoma but no evidence of tumor at the time of the washing. The incidence of positive staining in these groups was 0, 5, 78 and 0 per cent, respectively. The diagnostic value of immunoperoxidase staining was similar to that of Papanicolaou staining in the control group and in patients with high grade transitional cell carcinoma, and provided specific morphological criteria not possible by conventional cytology studies. Interpretation of immunoperoxidase staining was difficult in washings with a large number of inflammatory cells if endogenous peroxidase activity was not blocked properly. The application of the immunoperoxidase staining method for diagnosis of low grade tumor is under further investigation.

Antibodies, Monoclonal↗

PubMed2001

Modeling the two stages of surfactant-aided soil washing.

This paper provides new insights into modelling the distribution of hydrophobic compounds between soil and water phases in the presence of nonionic surfactant micelles. Experimental measurements were made of various systems comprising a non-ionic surfactant, five soils of different fractional organic carbon contents, and a hydrophobic (disperse) dye. Soil-washing performance was quantified using reciprocal surfactant-soil solubilization coefficients (1/Kd). Two stages of partitioning were identified. In stage 1, the dye concentration increased slightly with increasing surfactant dose until surfactant monomers saturated the bulk solution at the critical micelle concentration (cmc). The washing performance was 1:1 proportional to the surfactant monomer concentration. Most of the surfactant in this stage is sorbed. In stage 2, above the cmc, soil-washing performance increased linearly with increasing available surfactant micelles in the bulk solution. Reciprocal surfactant-soil solubilization coefficients (1/Kd), octanol-water partition coefficients (Kow), fractional organic carbon content of the soil (foc), and surfactant concentration were correlated for each stage in the soil-washing process using two simple equations.

Micelles↗

PubMed2002

Acid washing and stabilization of an artificial arsenic-contaminated soil.

An acid-washing process was studied on a laboratory scale to extract the bulk of arsenic(V) from a highly contaminated Kuroboku soil (Andosol) so as to minimize the risk of arsenic to human health and the environment. The sorption and desorption behavior of arsenic in the soil suggested the possibility of arsenic leaching under acidic conditions. Artificially contaminated Kuroboku soil (2830 mg As/kg soil) was washed with different concentrations of hydrogen fluoride, phosphoric acid, sulfuric acid, hydrogen chloride, nitric acid, perchloric acid, hydrogen bromide, acetic acid, hydrogen peroxide, 3:1 hydrogen chloride-nitric acid, or 2:1 nitric acid-perchloric acid. Phosphoric acid proved to be most promising as an extractant, attaining 99.9% arsenic extraction at 9.4% acid concentration in 6 h. Sulfuric acid also attained high percentage extraction. The arsenic extraction by these acids reached equilibrium within 2 h. Elovich-type equation best described most of the kinetic data for dissolution of soil components as well as for extraction of arsenic. Dissolution of the soil components could be minimized by ceasing acid washing in 2 h. The acid-washed soil was further stabilized by the addition of lanthanum, cerium, and iron(III) salts or their oxides or hydroxides which form insoluble complex with arsenic. Both salts and oxides of lanthanum and cerium were effective in immobilizing arsenic in the soil attaining less than 0.01 mg/l As in the leaching test.

Acids↗