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5-HT2 receptor-stimulated calcium influx in ovine uterine artery in late pregnancy.

The effects of 5-hydroxytryptamine (5-HT) and 2,5-dimethoxy-4-methyl-amphetamine (DOM) on the Ca2+ influx in the ovine uterine artery in late pregnancy have been studied by measuring 45Ca2+ uptake. Both 5-HT and DOM (2.5 x 10(-8) M to 2.5 x 10(-5) M) induced concentration-dependent rises in 45Ca2+ uptake in the ovine uterine artery, from a basal level of 30.5 +/- 3.5 muMoles/kg wet tissue to peak levels of 91.1 +/- 9.2 and 84.2 +/- 8.1 muMoles/kg wet tissue, respectively. The Ca2+ influx evoked by 5-HT and DOM was inhibited by ketanserin in a concentration-dependent manner. Methiothepin (2.5 x 10(-7) M) also inhibited 5-HT-induced (2.5 x 10(-6) M) Ca2+ influx by 86%. No antagonism was found with 2.5 x 10(-6) M of 3-tropanyl-3,5-dichlorobenzoate (MDL 72222). The contraction elicited to 5-HT (10(-6) M) and DOM (10(-6) M) was blocked by D600 (10(-6) M) which also blocked the contraction produced to KCl (9 x 10(-2) M). Amrinone (10(-5) M) had no inhibitory effect on these contractions. In accord with the results of the contraction study, D600 (2.5 x 10(-6) M) blocked the Ca2+ influx stimulated by 5-HT (2.5 x 10(-6) M) and amrinone (2.5 x 10(-5) M) failed to inhibit 5-HT-induced Ca2+ influx. Nifedipine (2.5 x 10(-6) M) did not antagonize the influx of Ca2+ induced by 5-HT. Sodium nitroprusside inhibited the 5-HT-induced Ca2+ influx, indicating that at least part of its inhibitory effect on the vasoconstriction evolved by 5-HT is due to the inhibition of the influx of Ca2+.

DOM 2,5-Dimethoxy-4-Methylamphetamine↗

Effect of domoic acid on metabolism of 5-hydroxytryptamine in rat brain.

Domoic acid (Dom) is a neurotoxic secondary amino acid that interacts with the glutamate receptors, producing neurological problems. In the present work, we study the effects of Dom on the levels of serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in discrete rat brain regions. The effects of Dom on the brain metabolism of serotonin are also discussed in this paper. Dom stimulates the rat brain serotoninergic system, increasing differentially the synthesis and the catabolism of 5-HT and the elimination of 5-HIAA.

Animals↗

Different effects of strenuous eccentric exercise on the accumulation of neutrophils in muscle in women and men.

The purpose of this study was to evaluate the sex differences in delayed onset muscle soreness (DOMS), torque, and accumulation of technetium-99m (Tc-99m) neutrophils in eccentric-exercised muscle. A group of 10 female and 12 male subjects took part in this study. The subjects completed a pre-test using the descriptor differential scale (DDS) to describe DOMS, and tests of concentric and eccentric torque of the right quadriceps. A volume of 100 ml of blood was taken by venipuncture for neutrophil labelling in the early morning of the exercise day. The Tc-99m neutrophils were re-infused intravenously before the eccentric exercise. The exercise stimulus consisted of 300 eccentric repetitions of the right quadriceps muscles. Radionuclide images of both quadriceps muscles (lateral views) were taken at 2 and 4 h. The DDS, and concentric and eccentric torques of the quadriceps were subsequently evaluated at 0 h, 2, 4, 20 and 24 h post-exercise. The presence of Tc-99m neutrophils was greater in the exercised leg than the non-exercised leg at 2 and 4 h post-exercise (P </= 0.013) and greater in the exercised leg of the women compared to the men at 2 h (P = 0.03). The DOMS had increased post-exercise (P < 0.001) and torque had decreased post-exercise (P </= 0.002) but the patterns were different between the sexes. We concluded that the sex influences the presence of Tc-99m neutrophils in the exercised muscle following eccentric exercise. In addition, different patterns of DOMS and torque were observed between the sexes after eccentric exercise, and require further investigation.

Adult↗

Metal distribution and binding in balneological peats and their aqueous extracts.

Binding of metals in typical bath peat samples ("Grosses Gifhomer Moor", Sassenburg/North Germany) and their aqueous extracts was characterized by means of a multi-method approach. For that purpose a sequential extraction procedure based on peat-filled chromatography columns was developed. Water-soluble metal and DOM (dissolved organic matter) fractions were subdivided by use of a stepwise increased pH gradient (pH 3.8-5), finally by the chelator EDTA and 0.1 mol L(-1) hydrochloric acid. Metal fractions very strongly bound to peat were assessed by an aqua regia extraction. Metal determinations required were performed by atomic spectrometry methods (AAS, ICP-OES, and TXRF). The metal and DOM concentrations in the peat extracts varied significantly, depending on the natural variety of the peat matter under study (e.g., Al: 25-674, Cd: 0.05-0.2, Cu: 5-15.4, Fe: 77-1785, Mn: 21-505, Ni: 2-33, Pb: < 1, Zn: 9-715 (microg L(-1)); Na: 8-45, K: 1.3-14.9, Ca: 2-51, Mg: 1.1-7.9 (mg L(-1)); 26-73 mg L(-1) DOC). An increase of the pH increased the DOC (dissolved organic carbon) of the peat extracts, but hardly the concentration of heavy metals. The latter could only be re-mobilized by EDTA and dilute hydrochloric acid. Additional investigations of the peat extracts using tangential-flow ultrafiltration revealed that the heavy metals extracted at pH < 4 were predominantly dissociated. At higher pH (pH > 4.5) they were preferentially bound to macromolecular DOM. Moreover, using multistage ultrafiltration the size distribution of the DOM and their metal species was assessed.

Algorithms↗

Effects of pig slurry on the sorption of sulfonamide antibiotics in soil.

Sorption of p-aminobenzoic acid (pABA) and five sulfonamide antibiotics to loess Chernozem topsoil amended with varied additions of pig slurry was investigated in batch trials. In unfertilized soil, partition coefficients (Kd) of sulfonamides ranged from 0.3 to 2.0. Strong sorption nonlinearity (1/n = 0.5 to 0.8) was best fitted by the Freundlich isotherm (R2 = 0.7 to 1.0) and was indicative for specific sorption mechanisms. Adsorption to pig slurry was much stronger, and nondesorbable portions were increased compared with soil. However, in a mixture of soil and slurry (50:1 w/w), sorption of the antibiotics was significantly decreased at a lower concentration range of pABA and the sulfonamides. This was attributed to competitive adsorption of dissolved organic matter (DOM) constituents from manure. An increase in pig slurry amendment resulted in increased total organic matter, DOM concentration, and ionic strength, but pH decreased. As a result, the nonadsorbed portions of pABA, sulfanilamide, and sulfadiazine (logD(ow) < -0.4) ranged from 47% to 82% of the applied concentration in the differently manured substrates. Dissolved fractions of the antibiotics reached a maximum at a soil-slurry ratio of 9:1 and decreased with further addition of manure. This decrease was related to the formation of less-effective DOM associates in solution. The adsorbed and desorbed portions of the less-polar substances--sulfadimidine, sulfadimethoxine, and sulfapyridine (logD(ow) > 0.1)--remained nearly constant in the presence of increased manure input. The pH changes caused by manure amendment strongly affected ionisation status of the latter compounds, thus resulting in increased adsorption, which compensated the mobilizing effect of DOM. It is suggested that the effect of manure be considered in test methods to determine the soil retention of pharmaceutical substances.

Adsorption↗

Effects of Microscale Water Level Fluctuations and Altered Ultraviolet Radiation on Periphytic Microbiota.

Abstract Microscale fluctuations in water level (1-20 mm) are common on a diurnal basis in shallow (<5-10 cm) wetlands, coupled to evapotranspiration losses during the daytime in excess of groundwater resupply. These depth variations alter the intensity of UV irradiance reaching attached periphytic algal and bacterial microbial communities. Effects of alterations of UV irradiance by micro-changes in water level on periphytic microbiota were examined experimentally. Attached microbial communities, grown on glass fiber filters in situ in a natural wetland, were exposed experimentally to near-natural levels of UV irradiance of differing spectral quality. UV intensity was altered by varying the distance of the communities from the light source, changes in UV-attenuating natural dissolved organic matter (DOM), and small changes in water level (2 or 4 mm). Algal productivity and photosynthetic oxygen production were significantly reduced by small enhancements of UV-B radiation, by decreased water levels of only 2 mm, and by reductions in concentrations of DOM. UV-B had only small short-term effects on chlorophyll a, although small increases in water depth and DOM concentration reduced pigment damage. Experimental removal of UV-B during in situ growth indicated that algae could adapt to UV radiation during growth in natural environments. Microbial oxygen consumption and bacterial productivity and biomass were also lowered significantly by UV-B exposure, and damage decreased with small (2 mm) increases in water depth or in DOM concentration. Selective inhibitors of algal photosynthesis and production of released extracellular organic substrates caused a concomitant reduction in bacterial productivity and a significant increase in magnitude of UV-B damage to bacterial biomass. These effects suggested that metabolic interactions between the periphytic autotrophs and heterotrophs altered community responses to UV-B radiation. Microscale water level reductions, common on a diurnal basis in shallow wetlands, and associated increased UV intensity can result in rapid alterations in periphytic metabolism.http://link.springer-ny.com/link/service/journals/00248/bibs/38n3p253.html</hea

Journal Article↗

The effects of a single bout of downhill running and ensuing delayed onset of muscle soreness on running economy performed 48 h later.

Delayed onset of muscle soreness (DOMS) is a common response to exercise involving significant eccentric loading. Symptoms of DOMS vary widely and may include reduced force generating capacity, significant alterations in biochemical indices of muscle and connective tissue health, alteration of neuromuscular function, and changes in mechanical performance. The purpose of the investigation was to examine the effects of downhill running and ensuing DOMS on running economy and stride mechanics. Nine, well-trained distance runners and triathletes participated in the study. Running economy was measured at three relative intensities [65, 75, and 85% of maximal aerobic capacity ( VO(2peak))] before (RE1) and 48 h after (RE2) a 30-min downhill run (-10%) at 70% VO(2peak). Dependent variables included leg muscle soreness, rate of oxygen consumption ( VO(2)), minute ventilation, respiratory exchange ratio, lactate, heart rate, and stride length. These measurements were entered into a two-factor multivariate analysis of variance (MANOVA). The analysis revealed a significant time effect for all variables and a significant interaction (time x intensity) for lactate. The energy cost of locomotion was elevated at RE2 by an average of 3.2%. This was coupled with a significant reduction in stride length. The change in VO(2) was inversely correlated with the change in stride length ( r= -0.535). Lactate was significantly elevated at RE2 for each run intensity, with a mean increase of 0.61 mmol l(-1). Based on these findings, it is suggested that muscle damage led to changes in stride mechanics and a greater reliance on anaerobic methods of energy production, contributing to the change in running economy during DOMS.

Adaptation, Physiological↗

Metabolic response to prolonged cycling with (13)C-glucose ingestion following downhill running.

The purpose of this study was to describe the effect of muscle damage and delayed-onset muscle soreness (DOMS) on the metabolic response during a subsequent period of prolonged concentric exercise (120 min, approximately 61% V(.)O(2max), on a cycle ergometer), with ingestion of 3 g of (13)C-glucose/kg body mass. We hypothesized that the oxidation of plasma and exogenous glucose would be reduced, while the oxidation of glucose arising from muscle glycogen would be increased. Six male subjects were studied during exercise in a control situation and 2 days following downhill running, at a time when plasma creatine kinase (CK) activity was increased, and DOMS was present. Carbohydrate and lipid oxidation were computed from indirect respiratory calorimetry corrected for protein oxidation, while the oxidation of plasma glucose and muscle glycogen were computed from V(.)(13)CO(2) and the ratio of (13)C/(12)C in the plasma glucose. All data were presented as the mean and the standard error of the mean. The oxidation of protein (approximately 6% energy yield, in the control and the experimental trial), lipid (approximately 15 and approximately 18%), and carbohydrate (approximately 79 and approximately 76%), as well as that of plasma glucose (approximately 41 and approximately 46%), glucose from the liver (approximately 12 and approximately 14%), and glucose from muscle glycogen (approximately 38 and approximately 31%) were not significantly different between the control and experimental (DOMS) trials. The response of the plasma glucose, insulin, lactate, and free fatty acid concentrations was not modified by the previous eccentric exercise. These results indicate that the metabolic response to prolonged concentric exercise is not modified by muscle damage and DOMS resulting from a bout of eccentric exercise performed 2 days before.

Adult↗

Ventilatory and circulatory responses at the onset of exercise after eccentric exercise.

The purpose of this study was to clarify whether delayed onset muscle soreness (DOMS) and muscle damage after eccentric exercise (ECC) could affect the ventilatory and circulatory responses at the onset of exercise, and whether those effects would continue after the disappearance of DOMS. Ten males participated in this study. We measured ventilatory and circulatory responses at the onset of exercise, for the first 20 s, during knee extension-relaxation voluntary exercise (VOL) and passive movement (PAS), which was achieved by the experimenter alternatively pulling ropes connected to the subjects' ankles for the same period and frequency as during VOL. VOL and PAS were performed before, 2 days after, and 7 days after ECC. The following results were found: (1) the gain of minute ventilation at the onset of VOL at 2 days after ECC was significantly larger than that of before ECC; (2) the gain of minute ventilation at 7 days after ECC during both VOL and PAS was also enhanced significantly as compared to that of before ECC; and (3) heart rate and blood pressure responses were unchanged throughout the experimental period. In conclusion, ventilatory response at the onset of exercise is augmented during DOMS and EIMD after ECC and the enhanced ventilatory response continued after the disappearance of DOMS. It is suggested that enhanced ventilatory response during exercise after ECC is attributed to at least the changes in neural factors and that the mechanisms inducing these augmented ventilatory responses should be different during the period after ECC.

Adult↗

Effect of exercise-induced muscle damage on the blood lactate response to incremental exercise in humans.

Eccentric muscle actions are known to induce temporary muscle damage, delayed onset muscle soreness (DOMS) and muscle weakness that may persist for several days. The purpose of the present study was to determine whether DOMS-inducing exercise affects blood lactate responses to subsequent incremental dynamic exercise. Physiological and metabolic responses to a standardised incremental exercise task were measured two days after the performance of an eccentric exercise bout or in a control (no prior exercise) condition. Ten healthy recreationally active subjects (9 male, 1 female), aged 20 (SD 1) years performed repeated eccentric muscle actions during 40 min of bench stepping (knee high step; 15 steps x min[-1]). Two days after the eccentric exercise, while the subjects experienced DOMS, they cycled on a basket loaded cycle ergometer at a starting work rate of 150 W, with increments of 50 W every 2 min until fatigue. The order of the preceding treatments (eccentric exercise or control) was randomised and the treatments were carried out 2 weeks apart. Two days after the eccentric exercise, all subjects reported leg muscle soreness and exhibited elevated levels of plasma creatine kinase activity (P < 0.05). Endurance time and peak VO2 during cycling were unaffected by the prior eccentric exercise. Minute volume, respiratory exchange ratio and heart rate responses were similar but venous blood lactate concentration was higher (P < 0.05) during cycling after eccentric exercise compared with the control condition. Peak blood lactate concentration, observed at 2 min post-exercise was also higher [12.6 (SD 1.4) vs 10.9 SD (1.3) mM; P < 0.01]. The higher blood lactate concentration during cycling exercise after prior eccentric exercise may be attributable to an increased rate of glycogenolysis possibly arising from an increased recruitment of Type II muscle fibres. It follows that determination of lactate thresholds for the purpose of fitness assessment in subjects experiencing DOMS is not appropriate.

Adult↗

Morphological and functional stimulation of adrenal reticularis zone by dopaminergic blockade in dogs.

In order to investigate factors affecting adrenal maturation leading to adrenarche, the effect of dopaminergic blockade on the morphology and function of the adrenal reticularis zone was studied in fourteen preadrenarchal dogs. Seven animals of 7 weeks of age (adrenarche at 11 weeks) were treated with domperidone (DOM) 3 times/day p.o. (3 mg/kg) at 09.00 h, 14.00 h, and 21.00 h, plus an additional injection of DOM s.c. (0.6 mg/kg) at 21.00 h for 15 days. Seven control animals received diluent. Twelve hours after the last injection, dogs received 0.25 mg ACTH i.m. and were sacrificed 60 min later. Blood was collected and adrenals removed. After histological evaluation, the percent of the reticularis, fasciculata, and glomerulosa zones with respect to the total cortex was calculated. Plasma androstenedione (A), dehydroepiandrosterone (DHA), 17-OH-progesterone (17P), and cortisol (F) levels in response to ACTH were also assessed. DOM-treated dogs show a significant development of the zona reticularis compared to control animals (19.2 +/- 0.6% vs 8.8 +/- 2.09% respectively, X +/- SE, P less than 0.01). In the same animals, the DHA response to ACTH (9.0 +/- 1.6 ng/dl) was significantly higher than in placebo-treated dogs (3.8 +/- 1.1 ng/dl, P less than 0.05) while no significant differences were found in F, A, and 17P levels between the two groups. Finally the post-ACTH A/DHA ratio of DOM-treated dogs (1.33 +/- 0.28) was significantly decreased in respect to the placebo-treated animals (5.49 +/- 2.54, P less than 0.01). These data demonstrate that the morphological and functional development of zona reticularis may be subject to dopaminergic control and this could represent an important step in the initiation of adrenarche. However, the cellular mechanism responsible remains to be clarified.

17-alpha-Hydroxyprogesterone↗

Continuous low-level heat wrap therapy for the prevention and early phase treatment of delayed-onset muscle soreness of the low back: a randomized controlled trial.

OBJECTIVE: To evaluate the effects of continuous low-level heat wrap therapy for the prevention and early phase treatment (ie, 0-48 h postexercise) of delayed-onset muscle soreness (DOMS) of the low back. DESIGN: Two prospective randomized controlled trials. SETTING: Outpatient medical facility. PARTICIPANTS: Sixty-seven subjects asymptomatic of back pain and in good general health (mean age, 23.5+/-6.6 y). INTERVENTIONS: Participants performed vigorous eccentric exercise to experimentally induce low back DOMS. Participants were assigned to 1 of 2 substudies (prevention and treatment) and randomized to 1 of 2 treatment groups within each substudy: prevention study (heat wrap, n=17; control [nontarget muscle stretch], n=18) and treatment study (heat wrap, n=16; cold pack, n=16). Interventions were administered 4 hours before and 4 hours after exercise in the prevention study and between hours 18 to 42 postexercise in the treatment study. MAIN OUTCOME MEASURES: To coincide with the expected occurrence of peak symptoms related to exercise-induced low back DOMS, hour 24 postexercise was considered primary. Pain intensity (prevention) and pain relief (treatment) were primary measures, and self-reported physical function and disability were secondary measures. RESULTS: In the prevention study, at hour 24 postexercise, pain intensity, disability, and deficits in self-reported physical function in subjects with the heat wrap were reduced by 47% (P<.001), 52.3% (P=.029), and 45% (P=.013), respectively, compared with the control group. At hour 24 in the treatment study, postexercise, pain relief with the heat wrap was 138% greater (P=.026) than with the cold pack; there were no differences between the groups in changes in self-reported physical function and disability. CONCLUSIONS: In this small study, continuous low-level heat wrap therapy was of significant benefit in the prevention and early phase treatment of low back DOMS.

Adult↗

Influence of dissolved organic matter on nickel bioavailability and toxicity to Hyalella azteca in water-only exposures.

Dissolved organic matter (DOM) is known to reduce the bioavailability of metals in aquatic systems. This study evaluated the effects of DOM from various sources (e.g., Little Bear Lake sediment, Suwannee River, peat moss) and various DOM fractions (humic acids, HA; fulvic acids, FA) on the bioavailability of nickel (Ni) to Hyalella azteca, a common freshwater benthic invertebrate. In particular, this study was conducted to evaluate the effect of surficial sediment DOM on Ni bioavailability. Short-term (48 h) acute toxicity tests with H. azteca conducted in synthetic water demonstrated that the aqueous Ni concentrations required for lethality were greater than what could be significantly complexed by environmentally relevant concentrations of dissolved organic carbon (DOC: 0.6-30.4 mg/L). At Ni concentrations sublethal to H. azteca (500 microg/L), the bioavailability of Ni was significantly reduced in the presence of representative surface water DOC concentrations regardless of DOC source or fraction. DOC fraction (i.e., FA and HA) differentially affected Ni speciation, but had little or no effect on Ni accumulation by H. azteca. Tissue Ni was found to be strongly dependent upon the Ni(2+) concentration in the exposure solutions and the Ni:DOC ratio. Overall, the concentration of DOC played a greater role than either DOC source or fraction in determining Ni speciation and hence bioavailability and toxicity to H. azteca.

Amphipoda↗

Effects of copper and cadmium on ion transport and gill metal binding in the Amazonian teleost tambaqui (Colossoma macropomum) in extremely soft water.

Metal toxicity in fish is expected to be most severe in soft waters because of the low availability of cations (particularly Ca(2+)) to out-compete the metal forms for binding sites on the gills. Natural waters in the Amazon basin are typically soft due to regional geochemistry, but few studies have focused on metal toxicity in fish native to the basin. We assessed the ionoregulatory effects of waterborne copper (Cu) and cadmium (Cd) on tambaqui (Colossoma macropomum) in extremely soft water (10 micromoll(-1) Ca(2+)). Tambaqui had a very high tolerance to Cu (50-400 microgl(-1)), as indicated by a complete lack of inhibition of Na(+) uptake and an ability to gradually recover over 6h from elevated diffusive Na(+) losses caused by Cu. The insensitivity of active Na(+) influx to Cu further supports the notion that Amazonian fish may have a unique Na(+) transport system. Addition of 5-10 mgCl(-1) of dissolved organic matter (DOM) did not prevent initial (0-3h) negative Na(+) balance in tambaqui exposed to Cu. Exposure to 40 mgCl(-1) DOM prevented Na(+) losses in tambaqui even at 400 microgl(-1) Cu, probably because most Cu was complexed to DOM. Tambaqui exposed to waterborne Cd (10-80 microgl(-1)) experienced an average of 42% inhibition in whole body Ca(2+) uptake relative to controls within 3h of exposure to the metal. Inhibition of Ca(2+) uptake increased over time and, at 24h, Ca(2+) uptake was suppressed by 51% and 91% in fish exposed to 10 and 80 microgl(-1) Cd, respectively. Previous acclimation of fish to either elevated [Ca(2+)] or elevated [DOM] proved to be very effective in protecting against acute short-term metal accumulation at the gills of tambaqui in soft water (in the absence of the protective agent during metal exposure), suggesting a conditioning effect on gill metal binding physiology.

Analysis of Variance↗

How accurately do semi-permeable membrane devices measure the bioavailability of polycyclic aromatic hydrocarbons to Daphnia magna?

Semi-permeable membrane devices (SPMDs) are passive samplers that have been designed to sample the bioavailable fractions of hydrophobic organic compounds in aquatic ecosystems. This study aims at evaluating the ability for SPMD to sample polycyclic aromatic hydrocarbons (fluoranthene, pyrene and benzo[a]pyrene) that are actually bioavailable to Daphnia magna. For that purpose, the SPMD-available fraction and the bioavailable fraction to D. magna are compared in controlled media with Dissolved Organic Matters (DOMs) from various origins and at different concentrations. The presence of all but one DOM reduces the accumulation of PAHs in SPMD or in D. magna. Moreover, this comparative laboratory study shows that in 10 cases on 13, the SPMD-available fraction is close to the available fraction to D. magna. When significant differences are observed between SPMD-available and bioavailable fractions, they remain less than 50% at DOM concentrations below 10mg/l DOC, which corresponds to a maximum DOC concentration usually found in temperate rivers. This study confirms the suitability of the SPMD technique to monitor readily bioavailable hydrophobic contaminants in aquatic environments containing DOM from various origins and characteristics.

Animals↗

Impacts of a heavy storm of rain upon dissolved and particulate organic C, N and P in the main river of a vegetation-rich basin area in Japan.

The impacts of a heavy storm of rain upon the dissolved and particulate organic matter (OM), nitrogen (N) and phosphorus (P) in the main river of the vegetation-rich Nagara River basin were investigated using water samples collected along the river line during a critical typhoon-induced heavy rain storm event. Besides, based on a high performance size-exclusion chromatography (HPSEC) system, the variance of dissolved OM (DOM) in its molecular weight (MW) characteristics was also assessed. From the MW standpoint, DOM components merged into the river along the river line resembled those present in its headwater. The MW range changed only slightly from 1010 to 5900 at the upstream (US), to 1130-5900 and 1200-5900 Da at the midstream (MS) and downstream (DS), respectively, while the corresponding weight-averaged MW (M(w)) decreased from 3669 to 3330 and 2962 Da. The heavy storm of rain enhanced the content of DOM; however, apart from a small larger-MW fraction (about 5900-6800 Da), the newly emerged DOM constituents exhibited an MW range similar to those existed before the storm. Due also to the storm of rain, total P and N (TP and TN) changed markedly in the ranges of 6.6-11.9, 8.3-40.6 and 48.4-231.3 microg/l for TP, and 145.4-296.0, 502.2-1168.7 and 1342.7-1927.3 microg/l for TN at the US, MS and DS, respectively. The larger values of TP and TN generally appeared for samples at elevated river water levels. The enhanced presence of P was found largely attributed to its particulate form; while, for N, the contribution from its dissolved form was significant. The newly emerged suspended particles via the storm-water contained lower content of OM, N and P, and a general decreasing trend of the particulate OM, N and P along the river line was also confirmed. The C/N ratio in the dissolved form varied in 0.7-6.7 and decreased downstream, while, that in the particulate form 2.3-17.3. Suspended particles that emerged in the river water during the storm exhibited larger C/N values. N/P in both dissolved and particulate forms varied in the ranges of 2.2-17.1 and 12.9-444.9, respectively, and a general trend of either increasing or decreasing in relation to the storm of rain was not revealed.

Agriculture↗

Enhanced coagulation in a typical North-China water treatment plant.

The characteristics of typical source waters in northern China and their enhanced coagulation features were studied in this paper. Through bench scale tests, a composite coagulant (HPAC) was selected for this kind of high alkalinity and pH water. It can be 30% more efficient in organic matter (OM) removal than the traditional coagulants (AlCl(3), FeCl(3)), and polyaluminum chloride (PACl), especially more efficient in removing high SUVA, hydrophobic and high molecular weight dissolved organic matter (DOM). It is found that some DOM with low SUVA has precedence over that with high SUVA to be removed at conventional dosages in some seasons, and that the priority of DOM removal is in the same sequence for all the coagulants. DOM with high SUVA is not always more easily removed. When applying HPAC as coagulant, flotation process can remove hydrophobic OM more efficiently than sedimentation process in pilot scale tests.

Aluminum Chloride↗

Microcolumn test and model analysis of activated carbon adsorption of dissolved organic matter after precoagulation: effects of pH and pore size distribution.

Microcolumn adsorption experiments were conducted to generate breakthrough profiles of dissolved organic matter (DOM) remaining after coagulation treatment of a naturally colored surface water for three coal-based activated carbons (ACs) and four water pH levels. A plug-flow homogeneous surface diffusion model was applied to determine the intraparticle surface diffusivities of the DOM at different AC-pH combinations. It was found that, for all three ACs, the removal of DOM increased as pH decreased and the increasing extent changed with the ACs used. The pH dependency of the column performance seemed to be attributed more apparently to pH's capability in changing the zeta potential of AC particles. In addition, at all pH levels, the column performance varied markedly with the ACs used. Correlation analyses of the accumulated amounts of DOM onto all three ACs with corresponding pore volumes in several divided pore size regions clearly indicated that pores with sizes 30-100 A were more effective in adsorbing organic macromolecules. Furthermore, based on model simulations, the sensitivity of bed performance to equilibrium and kinetic parameters was quantified by conducting variance analyses with a four-way classification method.

Journal Article↗