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[Pathogenicity of Spiroplasma sabaudiense (mollicute) for the cells (c6/36) of Aedes albopictus (Insecta: Diptera) in vitro].

An initial stock of Spiroplasma sabaudiense had been maintained in cell-free medium for 2-3 years. Subsequent passages in Leibovitz's medium L-15 in the presence of Aedes albopictus (C6/36) cells dramatically increased the pathogenicity of S. sabaudiense towards these cells. Cytopathogenicity included production of syncytia, an increase in the number of mitochondria and alteration of their morphology, vacuolisation and a reduction in the rate of growth. Cell lysis was associated with cytadsorption by spiroplasmas and was apparent following the third passage. At six days post-inoculation during the fourth and fifth passages, cell cultures had been totally destroyed by S. sabaudiense. The intra-cellular stage of S. sabaudiense is described and the literature pertaining to the pathogenicity of spiroplasmas for insects is reviewed.

Aedes↗

Systematics and evolution of the genus Paraceroglyphus and related taxa (Acari: Acaridae) associated with fleas (Insecta: Siphonaptera).

Collections of fleas from terrestrial Sciuridae from New Mexico and Montana yielded 2 species of acarid mites: Acarus monopsyllus from Ceratophyllus ciliatus and Paraceroglyphus cynomydis n. sp. from 4 species of Oropsylla. The genera Acarus, Paraceroglyphus, and Trichopsyllopus form a clade distinct from other genera of flea-associated mites, with Paraceroglyphus the sister group of the other 2 genera. Paraceroglyphus cynomydis is the sister group of a clade comprising P. xenopsylla and possibly P. californicus, with P. meles as the nearest outgroup.

Animals↗

Inhibition of the formation of protein storage granules by glutaurine in the larval fat body cells of Mamestra brassicae (Insecta, Lepidoptera).

Correlative changes in the protein contents of haemolymph and fat body and the accumulation of protein storage granules in the fat body cells of Mamestra brassicae were investigated during the last larval stage in normally developing larvae and following administration of glutaurine (1 X 10(-4) mg/g body weight). The protein content of the haemolymph of untreated larvae increased up to the 4th day of the stage, declined during days 5 and 6, and increased again before pupation. In the glutaurine-treated larvae the amount of proteins in the haemolymph was as high as in the controls during the first four days but continued to rise up to the end of the stage. The protein content of the fat body started to increase from the 3rd day and heavy accumulation of protein storage granules in the cells of fat body was observed on the 5th and following days. The protein content of the fat body of glutaurine-treated larvae remained at a low level and the protein storage granules were absent in the cells. The inhibition of the selective uptake of haemolymphatic storage proteins by fat body following glutaurine treatment is suggested.

Adipose Tissue↗

Peritrophic membrane formation and the midgut barrier to arboviral infection in the mosquito, Culex tarsalis Coquillett (Insecta, Diptera).

The formation of the peritrophic membrane in adult female mosquitoes, Culex tarsalis, was examined by light and electron microscopy. The initial evidence of secretion of peritrophic membrane precursors occurred at 8-12 h after ingestion of the bloodmeal. Morphogenesis of the peritrophic membrane occurred within 12-16 h after the initial secretion; culminating with a fibrous, multilayered peritrophic membrane 20-24 h after bloodmeal ingestion. A discussion of the potential participation of the peritrophic membrane in a midgut barrier to infection by arboviruses is presented. The participation of the peritrophic membrane in a midgut barrier to infection of C. tarsalis, and many other mosquito species, by arboviruses is considered a moot point.

Abdomen↗

Glycosphingolipids in insects. Chemical structures of ceramide tetra-, penta-, hexa-, and heptasaccharides from Calliphora vicina pupae (Insecta: Diptera).

Four neutal fraction glycosphingolipids, designated components 4-7, were purified from the pupae of Calliphora vicina and isolated by the use of high performance liquid chromatography. Their chemical structures were determined to be: GalNAc(beta 1-4)GlcNAc(beta 1-3)Man(beta 1-4)Glc(beta 1-1)Cer; GalNAc(alpha 1-4)GalNAc(beta 1-4)GlcNAc(beta 1-3)Man(beta 1-4)Glc(beta 1-1)Cer and Gal(alpha 1-3)GalNAc(beta 1-4)GlcNAc(beta 1-3)Man(beta 1-4)Glc(beta 1-1)Cer; Gal(beta 1-3)GalNAc(alpha 1-4)GalNAc(beta 1-4)GlcNAc(beta 1-3)Man(beta 1-4)Glc(beta 1-1)Cer; and GlcNAC(beta 1-3)Gal(beta 1-3)GalNAc(alpha 1-4)GalNAc(beta 1-4)GlcNAc(beta 1-3)Man(beta 1-4)Glc(beta 1-1)Cer. By the use of specific exoglycosidases, it was possible to assign anomeric configurations to all the sugar residues present. Analysis of the ceramide moiety by electron-impact mass spectrometry revealed the dominant fatty acid and sphingoid to be arachidic acid (C20:0) and tetradecasphing-4-enine, respectively.

Carbohydrates↗

[New data on the fleas (Insecta, Siphonaptera) of Algeria].

28 species or subspecies are noted; 6 are new for this country [Echidnophaga gallinacea (Westwood, 1875), Xenopsylla blanci Smit, 1957?, Ischnopsyllus intermedius (Rothschild, 1898), I. octactenus (Kolenati, 1856), Nycteridopsylla ancyluris Jordan, 1942 and N. pentactena (Kolenati, 1856)]. A new Caenopsylla is described in another paper.

Algeria↗

[Behavior and catecholamine levels: comparative study of gregarious insects, solitary insects, and those treated with carbon dioxide in Locusta migratoria (Insecta, Orthoptera)].

The dopamine (DA) and noradrenaline (NA) was measured in Locusta migratoria for 3 groups of individuals showing differences in their motility: gregarious are very active, solitary and animals in chronic treatment by CO2 (1 mn/day) show a very low motility. NA is present in small amounts (0,120-0,250 microng/g) in the 3 groups without significant differences. On the contrary, the quantity of DA is 5 times greater in gregarious than in the 2 other groups (1,78 and 0,31-0,39 microng/g). Thus it is suggested that DA is related to motility and must play its own role of a neurochormone, distinct from that of a metabolic intermediary between DOPA and NA. The question of a relationship between the metabolism of catecholamine and melanization is open.

Animals↗