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At least 523 records · Page 29Linked to original sources
PubMed1986

Destabilization of cytoplasmic mouse mammary tumor RNA by heat shock: prevention by cycloheximide pretreatment.

Thermal stress of cultured mouse mammary carcinoma cells results in a rapid loss of basal and glucocorticoid-stimulated mouse mammary tumor virus (MMTV) RNA. MMTV RNA is almost nondetectable within 1-2 hr after a shift in the culture temperature to 42 degrees. Actin mRNA is stable over this time period. Pretreatment of the cells with cycloheximide prevents the decay of MMTV RNA. Nuclear associated glucocorticoid receptor is not decreased but rather increased about 95% by the heat shock. The results show that heat shock has pronounced effects on expression of a glucocorticoid-regulated gene, and suggest that a nuclease, which is subject to rapid turnover, is stimulated by thermal stress.

Animals↗
PubMed1990

Demonstration of a new mouse mammary tumor virus locus in the genome of the mammary tumor prone strain SHN mouse.

Until this report, there have been no detailed analyses of the mouse mammary tumor virus (MMTV) loci in the mammary tumor prone strain SHN. Using a probe, which hybridizes to the env sequence of MMTV, Southern blotting of genomic DNA from the brain after digestion with EcoRI revealed 5 endogenous proviruses: Mtv-1 (4.5kb), Mtv-2 (11 kb), Mtv-8 (6.7kb), Mtv-17 (8.3kb) and a newly-found 6.5-kb fragment. F1-hybrid mice (C3H/He female x SHN male) also possessed the 6.5-kb fragment. Thus, we conclude that the 6.5-kb fragment is unique to SHN mice. Genomic DNA from mammary tumors of SHN mice showed MMTV insertions, suggesting that activation of an oncogene(s) occurred in this strain.

Animals↗
PubMed1983

Unusual findings of viral production (MuMTV) in murine mammary tumors (BALB/cfC3H).

Some unusual findings of the murine mammary tumor virus (MuMTV) are described in cells of BALB/cfC3H mice mammary tumors: (1) a conspicuous endocellular production of B particles by budding of A particles into small and large cytoplasmic vacuoles, into intracytoplasmic lumina, and possibly into vacuoles of secretion; (2) an extracellular production of B particles (in addition to the classic budding via microvilli) by opening at the cell surface of intracytoplasmic lumina containing mature and immature B particles and possibly of small vacuoles containing immature B particles. Morphological data suggest a virus excretion also by the secretory pathway of the neoplastic cell. Golgi complex is often seen in association with the small type of cytoplasmic vacuoles, whereas the large vacuoles are often close to a particular type of dense body, possibly lysosomes. The occurrence of free B particles in the cytoplasm is also reported. The possibility that the observed features are not occasional but are aspects of the normal life of the MuMTV is suggested.

Animals↗