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At least 523 records · Page 29Linked to original sources

[Fracture resistence force of the cement-expanded composite screw post-core system].

OBJECTIVE: This is a study comparing the effects of different post designs on the fracture resistence. METHODS: Twenty-seven simulated tooth roots made of polymethyl methacrylate (PMMA) were divided into three groups, then the cement-expanded composite screw post-core system (CECSP), cement screw post-core system (CSP) and cast metal post-core system (CMP) were manufactured. The root and post-core system was compressed by the speed of 1 mm/min on the Instron 4302 and the fracture resistence force was recorded. RESULTS: Statistical analysis of the measured values showed the fracture resistence force of CECSP and CMP to be significantly greater than that of CSP (P < 0.05), but there was no significant difference between the fracture resistence force of CECSP and CMP (P > 0.05). CONCLUSION: The fracture mechanical properties of CECSP are much better than those of cement screw post-core system and are similar to cast metal post-core system. Therefore, it is possible to find a new way for restoration of endodontally treated teeth.

Composite Resins↗

[Calcitonin as an alternative treatment for root resorption].

Inflammatory root resorption is a common finding following trauma and will cause eventual destruction of the tooth root if left untreated. This study examined the effects of intrapulpal application of calcitonin, a hormone known to inhibit osteoclastic bone resorption, on experimental inflammatory root resorption induced in monkeys. Results were histologically evaluated using a morphometric technique and revealed that calcitonin was an effective medicament for the treatment of inflammatory root resorption. It was concluded that this hormone could be a useful therapeutic adjunct in difficult cases of external root resorption.

Animals↗

Quantitative digital subtraction radiography for the determination of small changes in bone thickness: an in vitro study.

OBJECTIVE: The objective of this study was to determine the ability of quantitative digital subtraction radiography to detect small changes in bone thickness adjacent to tooth roots. STUDY DESIGN: A series of cortical or cancellous bone slices with a 50 microm-stepwise increasing thickness were attached to 4 porcine mandible sections covering buccal and interproximal "defect" regions. Standardized radiographs were quantitatively evaluated for radiographic density changes with the use of digital subtraction radiography. Furthermore, all radiographs were conventionally evaluated by 10 clinicians. The Wilcoxon signed-rank test and the Mann-Whitney U test were used for statistical analysis (alpha = 0.05). RESULTS: A high linear correlation was found between the actual thickness of bone slices and radiographic density changes (cortical bone: r2 = 0.89 to 0.99; cancellous bone r2 = 0.61 to 0.86, p < or = 0.001). A certain increase in bone thickness caused a 3 times higher increase in radiographic density for cortical bone than for cancellous bone (p < or = 0.05). The detection limits of digital subtraction radiography were 200 microm for cortical and 500 microm for cancellous bone, whereas the detection limits of conventional radiography were 600 microm and 2850 microm, respectively. CONCLUSIONS: This in vitro study demonstrated a very high correlation between the objective, quantitative assessment of subtle changes in alveolar bone by digital subtraction radiography and the true changes in bone thickness.

Absorptiometry, Photon↗

Bacterial endotoxin inhibits migration, attachment, and orientation of human gingival fibroblasts in vitro and delays collagen gel contraction.

The purpose of this study was to assess the effect of endotoxin adsorbed to dental surfaces and to collagen type I on the migration, attachment, and orientation of human gingival fibroblasts (HGF). Transversely cut porcine tooth root slices (RS), 200 micron thick, were prepared. Half of the RS obtained were partially demineralized in EDTA. Half of the demineralized and non-demineralized RS were incubated with 400 micrograms/mL of endotoxin for 24 hr, whereas the other half were maintained in PBS and served as controls. Experimental and control RS were placed on confluent layers of HFG and cultured for six days. Cell migration toward and cell attachment to the periphery of the RS and the formation of oriented cell sheets were assessed by means of photographic techniques. Additionally, six-day-old cultures were fixed and processed for SEM observation. In separate experiments, the effect of endotoxin on cell attachment to collagen type I and on contraction of three-dimensional collagen gels was assessed. It was found that: (i) bacterial endotoxin inhibited migration and attachment of HGF to both demineralized and non-demineralized cementum and interfered with the development of oriented cellular structure: (ii) the inhibitory effect was significantly more pronounced for non-demineralized than for demineralized cementum: (iii) the morphology of HGF attached to endotoxin-treated dental surfaces was altered compared with that of their controls: and (iv) bacterial endotoxin inhibited cell attachment to collagen type I and delayed the contraction of collagen gel.

Animals↗

ADAM28 participates in the regulation of tooth development.

Disintegrin and metalloprotease (ADAM) proteins are a family of membrane-anchored glycoproteins with diverse functions in fertilisation, development, neurogenesis and protein ectodomain shedding. ADAM28 is a newly discovered member of the ADAM family in humans and murine with autocatalytic activity. Recently, the authors screened ADAM28 genes from patients with congenital hypoplasia of tooth root, and studied the relationship between ADAM28 and tooth development. A polyclonal antibody (pAb) against ADAM28 was preparared, and the expression and localisation of ADAM28 were detected in tooth germ and dental mesenchymal cells. The results indicated that the prokaryotic expression vector pGEX-4T-ADAM28 was constructed successfully. Glutathione S-transferase-ADAM28 fusion protein was generated after inducement by isopropylthio-beta-d-galactoside and isolated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The purified fusion protein was used as an antigen for production of antibody. Western blot and enzyme-linked immunosorbent assay analyses verified that the antibody had a high specificity and titre. Immunohistochemistry and reverse transcriptase-polymerase chain reaction showed that ADAM28 was expressed at each stage of tooth germ development at different levels. Moreover, it was expressed in human dental follicle cells, human dental papilla cells, human dental pulp stem cells, human periodontal ligament cells and human dental cervical loop epithelial cells at transcription level. In conclusion, it is reasonable to suggest that ADAM28 may participate in tooth development and the regulation of odontogenic mesenchymal cells through progressive reciprocal inductive interactions between the epithelium and the mesenchyme.

ADAM Proteins↗

Effect of root canal sealers and irrigation agents on retention of preformed posts luted with a resin cement.

This study examined the effect of three sealers and four irrigants on retention of Dentatus preformed posts luted with Panavia 21 resin cement. Gutta-percha with one of the following sealers--EWT, AH26, Nogenol, or no sealer (control)--was used to fill the root canals of 160 autoclaved incisor and premolar tooth roots. After 72 hr 8 mm post spaces were created with Dentatus Probos Pathfinders and Dentatus reamers. After irrigating the post spaces with saline, 5.25% NaOCl, 50% citric acid followed by NaOCl, or 40% H3PO4, followed by NaOCl, Panavia 21 TC cement was introduced into the canal on the Dentatus #4 stainless steel post's threads. An up-and-down and counterclockwise motion and then a forward twist seated the posts without engaging dentin. Mesiodistal and faciolingual radiographs visualized the chambers. After 72 hr posts were removed axially with an Instron at 5 mm/min. Surprisingly, using Nogenol sealer reduced post retention whereas EWT and AH26 increased retention compared with no sealer. Post retention improved after acid etchants, especially for AH26.

Acid Etching, Dental↗

Fluoride profiles in the cementum and root dentine of human permanent anterior teeth extracted from adult residents in a naturally fluoridated and a non-fluoridated area.

OBJECTIVES: To determine the effect of water fluoride concentration on the fluoride profile across the entire thickness of the cementum and root dentine of human permanent anterior teeth in adults. SUBJECTS: Twenty-eight human permanent anterior teeth from individuals aged from 30 to over 60 years were studied. SETTING: Teeth were obtained from a natural high-fluoride area (West Hartle-pool, UK; 1.0-1.3 ppm F in drinking water, WHP) and the other from a non-fluoridated naturally low fluoride area (Leeds, UK; 0.1 ppm F in drinking water, LDS). DESIGN: Cementum and root dentine were sampled using an abrasive micro-sampling technique from the cementum surface to the pulpal surface of root dentine. RESULTS: Fluoride concentration was higher in tooth roots (the cementum and dentine) taken from the naturally fluoridated area (WHP) than from the non-fluoridated area (LDS). Age and average fluoride concentration showed a positive correlation in WHP dentine, middle region of the root (r = 0.78, P < 0.001) and in the apical region of the root (r = 0.61, P < 0.05). WHP cementum had the strongest fluoride concentration correlation with age in the cervical region of the root (r = 0.67, P < 0.01). An analysis of variance (ANOVA) showed that the area (water fluoride content), age and number of years lived in the area combined with total age were significant. CONCLUSIONS: The fluoride content of cementum and root dentine in adult residents is related to fluoride content in drinking water.

Adult↗