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PubMed2005

BAF complex is closely related to and interacts with NF1/CTF and RNA polymerase II in gene transcriptional activation.

Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg-associated factor complexes. In the present study, we investigated the spatial relationship between Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase II (RNAP II) upon gene transcriptional activation in vivo by employing immuno-gold labeling. The data showed that Brg1 was closely co-localized with NF1/CTF and RNAP II in HeLa cells. Moreover, the co-immunoprecipitation assay further revealed that Brg1 can be isolated together with NF1/CTF and RNAP II in the ConA-stimulated, but not the resting, T lymphocyte. The combined results suggested that BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription.

Animals↗
PubMed2005

hZimp7, a novel PIAS-like protein, enhances androgen receptor-mediated transcription and interacts with SWI/SNF-like BAF complexes.

Members of the PIAS (protein inhibitor of activated signal transducer and activator of transcription) family are negative regulators of the Janus family of tyrosine kinase (JAK)-signal transducer and activator of transcription pathway. Recently, PIAS proteins have been shown to interact with multiple signaling pathways in various cellular processes, and it has been demonstrated that PIAS and PIAS-like proteins interact with nuclear hormone receptors. In this study, we have identified a novel human PIAS-like protein, provisionally termed hZimp7, which shares a high degree of sequence similarity with hZimp10 (human zinc finger-containing, Miz1, PIAS-like protein on chromosome 10). hZimp7 (human zinc finger-containing, Miz1, PIAS-like protein on chromosome 7) possesses a molecular mass of approximately 100 kDa and contains a conserved Miz (msx-interacting zinc finger) domain, a nuclear translocation signal sequence, and a C-terminal transactivation domain. Northern blot analysis revealed that hZimp7 is predominantly expressed in testis, heart, brain, prostate, and ovary. Moreover, immunohistochemical staining of prostate tissues revealed that endogenous hZimp7 protein localizes to the nuclei of prostate epithelial cells and costains with the androgen receptor (AR). Further analysis of hZimp7 subcellular localization revealed that hZimp7 and the AR colocalize within the nucleus and form a protein complex at replication foci. Transient transfection experiments showed that hZimp7 augments the transcriptional activity of the AR and other nuclear hormone receptors. In contrast, reduction of endogenous hZimp7 protein expression by RNA interference decreased AR-mediated transcription. Finally, we determined that hZimp7 physically associates with Brg1 and BAF57, components of the ATP-dependent mammalian SWI/SNF-like BAF chromatin-remodeling complexes. The above data illustrate a potential role for hZimp7 in modulation of AR and/or other nuclear receptor-mediated transcription, possibly through alteration of chromatin structure by SWI/SNF-like BAF complexes.

Animals↗
PubMed2002

Distinct effects of thrombopoietin depending on a threshold level of activated Mpl in BaF-3 cells.

Thrombopoietin (TPO) plays a critical role in megakaryopoiesis through binding to its receptor Mpl. This involves activation of various intracellular signaling pathways, including phosphoinositide 3-kinase (PI3K) and the mitogen-activated protein kinase (MAPK) pathways. Their precise role in TPO-mediated proliferation, survival and differentiation is not fully understood. In the present study, we show that TPO induces different biological responses in Mpl-transduced BaF-3 cells, depending on the cell surface density of Mpl and the resulting activation level of signaling pathways. TPO mediates cell proliferation in cells expressing high levels of Mpl but only mediates survival without proliferation in cells expressing low levels of the receptor. By using the kinase inhibitors PD98059 and LY294002, we further showed that the activation level of the PI3K and MAPK p42/44 pathways is a determining factor for the proliferative effect. In cells expressing low levels of Mpl, the survival effect was strongly dependent on the activation level of the PI3K/AKT, but not the MAPK p42/44 pathway. Moreover, this effect was correlated with the phosphorylation level of BAD but not with the expression level of Bcl-X(L). However, PI3K pathway inhibition did not increase apoptosis when BaF-3 cells proliferated in response to TPO, indicating a compensating mechanism from other Mpl signaling pathways in this case.

Animals↗
PubMed2003

[The nitrite accumulation in a biological aerobic filter (BAF) and the influences factors].

The nitrite accumulation in a bench-scale biological aerobic filter (BAF) for nitrogen removal was reported in this paper. The influences of operational variables on filter performance and nitrite accumulation were also investigated. The influent loading of NH4+(-)N and TN were 0.26-0.62 kg/(m3.d) and 0.28-0.63 kg/(m3.d) respectively, the hydraulic loading, gas/water ratio and temperature were 1-2 m/h, 3:1 and 20.5 degrees C-26.5 degrees C respectively, the nitrogen removal loading of NH4+(-)N, TN were among 0.15-0.52 kg/(m3.d) and 0.18-0.42 kg/(m3.d) respectively. The nitrite concentrations in the bulk and effluent, the profiles of inorganic nitrogen-compounds, the microbial populations in the reactor and their activity were investigated, suggesting that the nitrite accumulation and shortcut nitrification-denitrification took place in the bioreactor. The mechanism of nitrite accumulation and the influences of operational variables were analyzed. The backwash process was considered as the key influential factor of nitrite accumulation. It was believed that the structure feature and operational pattern were the principal factors that may result in the nitrite accumulation and shortcut nitrification-denitrification occurring in the BAF reactor.

Bacteria↗
PubMed2006

[Biodegradation of oil field wastewater in biological aerated filter (BAF) by immobilization].

A special kind of carrier was used to immobilize effective microorganisms B350M in a biological aerated filter (BAF) react system for treatment of oil field wastewater, which is of salinity > 0.5%, lack of N and P, and contains low organic matter. Through the biodegradation system operated for 142d, the react system can achieve average degradation efficiency 90.5%, 74.4%, 85.6%, 100% for oil, TOC, COD and H2S, when HRT was 4h and COD volumetric load was 1.07 kg/(m3 x d). GC-MS results show that the organic substance in wastewater contain 27 different kind substances, a majority (23) of alkane and a minority (4) of aromatic substances. C14H30 to C28H58 in influent could be decomposed into small molecular substance efficiently, especially the C18H38 to C28H58, and also polycyclic aromatic hydrocarbons (PAHs) such as Phenanthrene. The react system had a good diversity, because the carriers provide agreeable air and water condition for microorganisms, to resist high salinity and toxic pollutant. Filamentous microorganisms were observed in a great deal and will not cause foaming and bulking in BAF reactor by immobilization.

Biodegradation, Environmental↗
PubMed2002

Novel competitive irreversible inhibitors of aldehyde dehydrogenase (ALDH1): restoration of chemosensitivity of L1210 cells overexpressing ALDH1 and induction of apoptosis in BAF(3) cells overexpressing bcl(2).

4-Amino-4-methyl-pent-2-ynthioc acid S-methyl ester (ampal thiolester: ATE) was used as a lead compound to synthesise new amino-substituted derivatives of alpha, beta acetylenic thiolester compounds as inhibitors of aldehyde dehydrogenase 1, (ALDH1). Of these compounds, the dimethyl derivative (DIMATE) was a competitive irreversible inhibitor (K(i) approximately 280 microM) of baker's yeast ALDH1 in vitro showing 80% inhibition at 400 microM when preincubated with the enzyme for 30min, whereas the trimethyl ammonium and the morpholine derivatives showed only 15% inhibition at 600 microM even after 60min preincubation. ATE inhibited ALDH1 activity in ALDH1-transfected L1210 T cells resistant to hydroperoxycyclophosphamide (HCPA) and inhibited growth synergistically in the presence of HCPA. In non-transfected L1210 counterparts ATE did not potentiate growth inhibition by HCPA. DIMATE was a 30-100-fold more effective growth inhibitor than ATE. Endogenous ALDH1 activities of BAF(3) cells over-expressing different levels of bcl(2) (0-100%) were similar (16-20mU/mg protein) and were all inhibited by DIMATE, reaching 20-30% at 4 microM. Up to 4 microM no apoptosis, as measured by DNA-fragmentation was observed, but at 8 and 10 microM DIMATE, DNA-fragmentation increased concomitantly with ALDH1 inhibition. No DNA-fragmentation was observed with ALDH1 irreversible inhibitors devoid of a thiolester group or with thiolesters which were not inhibitors of ALDH1. It was seen only with competitive irreversible inhibitors having the methanethiol and enzyme-inhibitory moieties. The methanethiol putatively released from DIMATE by ALDH1 esterase activity plays a role, albeit undefined, in lowering intramitochondrial glutathione levels which decreased by 47% as DNA-fragmentation increased.

Aldehyde Dehydrogenase↗
PubMed1999

Predicting bioconcentration factors (BCFs) of polychlorinated bornane (Toxaphene) congeners in fish and comparison with bioaccumulation factors (BAFs) in biota from the aquatic environment.

Polychlorinated bornanes, the main components of Toxaphene, are bioconcentrated in aquatic organisms to a high extent. However, up to this time no bioconcentration tests with individual chlorinated bornanes in aquatic organisms have been performed. Therefore, the bioconcentration factors (BCFs) of seven selected persistent chlorinated bornane congeners which are regularly found in aquatic organisms, were predicted from their n-octanol/water partition coefficients (log Kow). Furthermore, these BCF values were compared with the measured bioaccumulation factors (BAFs) in zooplankton and different fish species from the aquatic environment.

Animals↗
PubMed1997

In bone marrow derived Baf-3 cells, inhibition of apoptosis by IL-3 is mediated by two independent pathways.

The inhibition of cell death by growth factors plays a key role in the maintenance of the haematopoietic system homeostasis. However the mechanisms involved in this inhibition are still poorly understood. In order to determine if inhibition of apoptosis by growth factors is dependent only on the expression of survival genes, we have studied that process in the bone marrow derived IL-3 dependent cell line Baf-3. We show that, following IL-3 starvation, mRNA and protein levels of Bcl-X but not Bcl-2 decrease rapidly preceeding the onset of death. The death of IL-3 starved cells is asynchronous, starting between 6 to 8 h with 50% death being reached after 10 to 12 h. At any time point, apoptosis can be rapidly inhibited by growth factor re-addition. This has allowed us to determine that the inhibition of apoptosis by growth factor takes place at two levels. The first one, which we have called short term inhibition, is independent of mRNA and protein synthesis i.e. it takes place in the absence of survival gene neosynthesis and can be demonstrated during the first 6 h following growth factor re-addition. The second one corresponds to long-term survival-more than 24 h survival-and is strongly correlated with the induction of Bcl-X but not Bcl-2 gene expression. This induction of Bcl-X by IL-3 is shown to be dependent on MAP-kinase activation.

Animals↗
PubMed2006

Isotopic and internal CX3 (X = D,H) rotational motion effects in the Ba...FCX3 + h nu --> BaF + CX3 intracluster reactions.

Photodepletion and action spectra of the laser-induced Ba...FCD3 fragmentation have been measured over the 16 075-16 380 cm(-1) range. The observed band and peak structures allowed us to estimate the vibrational and rotational structures of the excited complex at the transition state configuration. The relative reaction probability P(R)(E) for the intracluster Ba...FCD3 + h nu --> BaF + CD3 reaction has been determined over the cited energy range. P(R)(E) shows a peak structure with an energy spacing of 8.9 cm(-1) which was attributed to an internal rotation of the CD3 group in the intermediate state. A comparison with previous Ba...FCH3 photofragmentation spectra reveals the dynamical role of the internal CX3 (X = H,D) motion which is manifested by the presence of rotational resonances in the laser-induced intracluster reaction.

Journal Article↗
PubMed2002

Phosphatidylinositol-dependent actin filament binding by the SWI/SNF-like BAF chromatin remodeling complex.

Recently, several chromatin remodeling complexes in yeast, Drosophila, and mammals have been shown to contain actin and actin-related proteins (arps). However, the function of actin in these complexes is unclear. Here, we show that the mammalian SWI/SNF-like BAF complex binds to phosphatidylinositol 4,5-bisphosphate (PIP2) micelles and PIP2-containing mixed lipid vesicles, and that PIP2 binding allows the complex to associate with actin pointed ends and branch points. Actin binds to at least two distinct domains in the C terminus of the Brg1 protein, and interaction with only one of these domains is sensitive to PIP2. Based on these findings, we propose a model for PIP2 activation of actin binding by relief of intramolecular capping of actin by Brg1.

Actin Cytoskeleton↗
PubMed2001

Overexpression of E2F-1 leads to cytokine-independent proliferation and survival in the hematopoietic cell line BaF-B03.

Cytokine receptors activate signals that regulate the transcription factor E2F-1, which then coordinates the expression of genes essential for DNA synthesis and cell cycle progression. Overexpression of E2F-1 most often induces S-phase entry followed by apoptosis, but in some cell types it leads to continuous proliferation and transformation. Here, it is shown that constitutive expression of E2F-1 promotes cytokine-independent proliferation in the murine pro-B cell line BaF-B03. There was no enhancement of apoptosis following cytokine withdrawal in these cells, despite the presence of intact p53-dependent apoptotic pathways. Notwithstanding the continuous presence of E2F-1, the cell cycle-dependent expression of cyclin A, cyclin B1, cyclin D1, cyclin E, and proliferating-cell nuclear antigen was restored with a pattern equivalent to that associated with cytokine stimulation. These findings provide evidence that, in the absence of cytokine, constitutive expression of E2F-1 can promote cell cycle progression and prevent apoptosis.

Animals↗
PubMed2001

Experience with an up-flow biological aerated filter (BAF) for tertiary treatment: from pilot trials to full scale implementation.

Biofilters can be added to existing non-nitrifying activated sludge plants for tertiary ammonia removal and effluent polishing. It is a convenient, efficient, and cost effective way of meeting more stringent consents. In order to prove this technology, a biofilter pilot plant was installed in a large activated sludge plant with challenging conditions, since almost half of the load is industrial effluent and the water temperature can be as low as 7 degrees C. Trials were conducted over a two year period, providing the following information: low water temperature does not affect the process; the optimum rise rate at nominal flow is 8.5 m/h for this wastewater; the consent can be achieved for ammonia loads up to 0.94 kgNH4N/M3 media/day; about 50% of the post-secondary TSS and BOD can be removed in the BAF; a large proportion of industrial effluent has not had any noticeable effect on the beads after two years. A full scale plant with a nominal flow of 8257 l/s was then built and commissioned in summer 1998. Seeding was completed within three weeks of starting the filters. Two months later, a 28 day takeover was started. Results obtained on full scale plant during and after this test confirm the results obtained on the pilot.

Ammonia↗
PubMed2002

Comparison of combined and separated biological aerated filter (BAF) performance for pre-denitrification/nitrification of municipal wastewater.

The performance of two systems of semi-industrial up-flow biological aerated filters (BAF) with pre-denitrification followed by nitrification was studied and compared under various operating and loading conditions. The first system consisted of two separate reactors for the denitrification and the nitrification step, whereas in the second system the aerobic nitrification zone was packed on top of the anoxic denitrification zone in one reactor. The second system potentially offers substantial savings in investment costs and space requirements for a large scale treatment plant. Regarding the elimination of carbonaceous pollution and denitrification the systems did not show significant differences. However, nitrification in the combined system suffered from the mixing of different biocenosis by daily backwashing and was reduced to 50-70% of the separated system's performance. Factors such as oxygen concentration, raw water composition and loading rates affected both systems' nitrification rates in similar ways. Since it is impossible to optimise the nitrification and denitrification processes separately, the combined system should only be considered for large scale applications if space is very scarce and if a stable raw water composition can be expected. If strict limit values for nitrate have to be met in the effluent, a combination of pre- and post-denitrification is advantageous and advisable.

Bacteria, Aerobic↗
PubMed2005

[Progress of biological air filter (BAF) development in manned spacecraft cabin].

The contaminants originating from human metabolism, material off-gassing and waste processing, may influence human health and the growth and development of higher plants when they accumulate at some degree in the spacecraft cabin. So the contaminants concentrations must be controlled below the spacecraft maximum allowable concentration (SMAC). For the long manned space missions and planetary habitation, biological technique is available for the removal of the contaminants. The biological air filter, BAF, is a system that degrades the contaminants into carbon dioxide, water and salts. It holds many advantages such as small weight and volume, low power consumption, easy maintenance and good working performance under the condition of microgravity. Its wide application will be seen in the space field in near future.

Air Conditioning↗
PubMed1982

An improved specimen table for the Balzers freeze etching system BAF 400.

A time saving, simple and inexpensive adaptation of a Balzers specimen table is described. This specimen table is used in combination with the small specimen holders originally developed for the Denton Freeze-etching module. They are in particular suited for cell suspensions, and are easy to handle for freezing procedures. Ten replicas are produced routinely per freeze fracture run, with the aid of this combined system.

Blood Platelets↗