PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Basophil Degranulation Test”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

[Clinical performance of the basophil degranulation test. Correlation with other diagnostic technics in allergy (II)].

Although the clinical history is the fundamental method for the diagnosis of reaginic diseases, the use of complementary techniques that gears on the detection of the humoral and cellular effector cells intervening in such affectation is necessary. Thus, we can detect the existence of specific IgE. It can be joined to the basophil membrane by means of techniques "in vivo" as in cutaneous tests, or by means of techniques "in vitro" through cellular techniques in which we apply a biochemical pattern (release of histamine) or a morphological one (HBDT). It can also be free in serum by means of the P-K test or "in vitro" by means of techniques of double antibody. We can also determine, by this last technique, the quantity of the total IgE in serum. In the present work, we review the human basophil degranulation test (HBDT) and its correlation with the prick test, the peripheral blood eosinophilia, the total serum IgE quantification by enzymatic technique and the specific IgE detection by isotope method. In our technique the test was considered positive when the degranulation index with known allergen concentration was greater than 30% for pollens and greater than 25% for D. pteronyssinus with a p less than 0.001. As reported in a previous work, we explain that the normal limits are slightly inferior to those used by some other authors, because in our series, the degranulation average of healthy controls is inferior to that reported by those authors which makes us consider the convenience that the normal limits of degranulation index should be established in a particular way by each technique and allergen.(ABSTRACT TRUNCATED AT 250 WORDS)

Basophils↗
PubMed1987

Human basophil degranulation test in liver hydatidosis.

Upon exposure to a specific antigen, granulation of basophils bearing IgE disappears. The aim of this study was to assess the human basophil degranulation (HBD) test with hydatic antigen in 100 patients. Seventy patients were clinically suspected of having liver hydatidosis (LH) (group I). Eight patients had asymptomatic hepatic calcifications. Twenty-two patients had previous surgery for LH (group III). In group I the percentage of HBD was significantly higher in patients with LH (23 of 70) than in patients with other hepatobiliary diseases (P less than 0.001). In group II all patients had negative HBD tests. In group III the test became negative within 8-12 months after radical surgery in four patients, and remained positive in the other 18. These results suggest that the HBD test is useful for the diagnosis of LH. In asymptomatic patients with hepatic calcified cyst, a negative HBD test suggests a dead parasite. In previously operated patients, a positive test suggests persistence of antigen.

Animals↗
PubMed1981

[The human basophils degranulation test (HBDT). Leucoconcentration and statistical calculation applied to the degranulation rate (author's transl)].

The technique using hemolysis and vital coloration described by Benveniste permits the human basophils to be studied in immediate type allergies : HBDT. In almost 25% of the cases, the test cannot be carried out because the basophils are less than 20 per mm(3). In 700 but one case, the HBDT could be carried out using a leucoconcentration technique by means of a 100 to 200 g centrifugation of 10 mn duration, followed by tangential extraction, thus obtaining an increased number of basophils. The authors were lent to apply a statistical calculation to a significant degranulation rate due to the number of observable cells by means of an unilateral statistical test suggesting a distribution according to Poisson's law. Thus tests are interpreted more rapidly and are more reliable. On the other hand, comparison with the results obtained with or without leucoconcentration in different types of allergies due to reagins (drugs, hymenoptera, foods) do not show any cellular fragilization. It may be concluded to the reliability of the degranulation observed in presence of an antigen.

Basophils↗
PubMedDate not supplied

[Human basophil degranulation test (HBDT) for diagnosis of respiratory allergic disorders. A study of eighty-four HDBT performed in forty-seven patients (author's transl)].

The HBDT uses basophils of patients with respiratory allergic disorders. In this simple method the basophils are stained by toluidine blue and the erythrocytes are destroyed. The basophils are then exposed to solutions containing different concentrations of the antigen. The basophil degranulation rate in response to a given antigen can be determined. The test is positive if the degranulation rate is over 50% and negative if the rate is under 30%. Our clinical study induces 47 patients. The correlate rate between HBDT and skin tests is 81,5%. This test's technical simplicity allows it's routine use by a non specialized laboratory.

Adolescent↗