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At least 55 records · Page 3Linked to original sources

Impotence in the boar: angioarchitecture and venous drainage of the penis in normal boars.

Twenty penis from normal boars were injected with various media at known reservoir pressures and examined by radiography, dissection, maceration, clearing and sectioning. Injections into the corpus spongiosum penis (csp) produced dorsal deviation and clockwise spiral twisting. The ccp was traversed by a system of large longitudinal cavernous spaces. The csp consisted of one large vascular space, its walls joined by trabeculae which were close together ventrally and further apart dorsally. The corpus spongiosum glandis (csg) was a vascular network within the penile integument communicating with the csp by fine vascular spaces around the urethral orifice. The penile arteries and veins were supplied and drained by the internal pudendal vessels. The ccp was drained from its crura by the v profunda penis but no venous drainage of this cavernous body was demonstrated within the body of the penis. The csp was drained from its bulb by the v bulbi penis; its distal extremity was drained by two large veins which ran proximally enclosed by the tunica albuginea of the csp. The right vein was always much larger than the left vein and at the sigmoid flexure the two united to form the right tributary of the v dorsalis penis. The csg and the entire penile integument were drained by the left tributary of the v dorsalis penis; the csg was also drained by one or both of the ventral veins of the csp. Blood from the scrotum was drained by the v circumflexa perinealis and v perinealis ventralis into the v dorsalis penis and v penis. A large dorsal lymphatic vessel drained lymph from the body of the penis into the pelvic lymph nodes. No connections with the superficial inguinal lyphocentres were identified.

Animals↗

Breed differences in boar taint: relationship between tissue levels boar taint compounds and sensory analysis of taint.

A total of 228 intact male pigs form Duroc, Hampshire, Landrace, and Yorkshire breeds were used in the experiment. Samples of salivary gland and backfat were collected at slaughter for colorimetric assay of salivary and fat 16-androstene levels and fat skatole levels. Fat levels also were tested by a sensory panel using an R-index technique for detecting the presence of boar taint. The proportion of tainted carcasses determined by the sensory panel was 5.0% for androstenone and 11.4% for skatole, with a combined total of 15.0% tainted from either source. Sensory analysis of taint showed a lower proportion (P < .05) of tainted carcasses in Hampshire, with no difference in taint across the other three breeds. Analysis of taint compounds indicated that overall 14.5% of pigs had salivary gland 16-androstene levels and 20.9% had fat 16-androstene levels above acceptable limits. There was a higher (P < .05) proportion of Duroc pigs above the threshold levels for 16-androstenes in both salivary gland and fat. Landrace pigs had the lowest (P < .05) average tissue concentrations of steroids and skatole. Across breeds, only 1.8% of pigs had fat skatole concentrations above .25 ppm, which has been suggested as threshold levels of skatole for taint. The canonical correlation coefficient between fat compound levels and the R-indices of fat 16-androstenes and skatole was .40 (P < .001). Our results indicate breed differences in tissue levels of taint compounds and in taint assessed by a sensory panel. Levels of 16-androstene steroids were highly associated with taint, but more pigs had measured levels above the threshold than those identified as tainted by sensory analysis. Levels of fat skatole were low overall and did not account for all the pigs judged as tainted from skatole by sensory analysis.

Androstenes↗

Cryopreservation of boar semen. III: Ultrastructure of boar spermatozoa frozen ultra-rapidly at various stages of conventional freezing and thawing.

Ejaculated boar spermatozoa subjected to a conventional freezing and thawing process, were ultra-rapidly fixed, freeze-substituted and examined by electron microscopy to monitor the presence of real or potential intracellular ice and the degree of cell protection attained with the different extenders used during the process. Numerous ice crystal marks representing the degree of hydration of the cells were located in the perinuclear space of those spermatozoa not in proper contact with the extender containing glycerol (i.e. prior to freezing). The spermatozoa which were in proper contact with the extenders presented a high degree of preservation of the acrosomes, plasma membranes as well as the nuclear envelopes. No ice marks were detected in acrosomes before thawing, indicating that the conventional assayed cryopreservation method provided a good protection against cryoinjury. The presence of acrosomal changes (internal vesiculization, hydration and swelling) in thawed samples however, raises serious questions about the thawing procedure employed.

Animals↗

Boar acrosin. Isolation of two active forms from boar ejaculated sperm.

A simple method avoiding the use of nonionogenic detergent has been developed for the isolation of two forms of boar acrosin from ejaculated sperm. Each of the two forms was electrophoretically homogeneous and showed one N-terminal sequence only; Val-Val. The two acrosin forms isolated differ in molecular mass and amino acid composition. alpha-Acrosin, the form showing a higher molecular mass (Mr approximately 50 000), is stable in acid media. When exposed to pH above 4 it is converted into beta-acrosin (Mr approximately 35 000) by autodigestion. The isolation of the alpha-form was therefore carried out below pH 4 to eliminate autodigestion to the beta-form. The beta-form is stable for a certain period at neutral pH, especially if stabilized by Ca2+ ions. The autodigestion of alpha-acrosin to beta-acrosin probably results in the liberation of the C-terminal portion of the molecule of the alpha-form; this portion is composed of roughly 85 residues and is rich in proline.

Acrosin↗

Relationship between androstenone content in fat, intensity of boar taint and size of accessory sex glands in boars.

Coefficients of correlation in the range of 0.6-0.8 were found between the size of the accessory sex glands and level of androstenone in subcutaneous fat. The length and weight of accessory sex glands combined in a multiple regression equation explained 64% of the variation in androstenone content. The correlation between boar taint intensity and size of accessory sex glands varied between 0.28 and 0.34.

Adipose Tissue↗

[Quantifiable morphokinesis of parts of the hypothalamo-adenohypophyseal-gonadal axis in hormonally castrated boars. 3. Studies of boars following treatment with steroid test substance 547].

Morphokinetic effects caused in parts of the hypothalamic adenohypophyseogonadal axis of boars by treatment, using No. 547 steroid test substance (ZIMET, Jena) were to some extent identical with those resulting from analogous CAP application (inhibition of testicular androgen producers, anterior lobe of the pituitary gland, nucleus praeopticus medialis) or those following analogous norgestrel treatment (testes weight, tubule length). The findings recorded were largely attributed to the androgenic properties of No. 547 steroid test substance.

Androstenes↗

[Quantifiable morphokinesis on parts of the hypothalamo-adenohypophyseal-gonadal axis of hormonally castrated boars. I. Studies on boars treated with chlormadinone acetate].

A clearly morphokinetic effect was recorded from the parts of the hypothalamo-adenohypophyseo-gonadal axis in boars that had been treated with chloromadinone acetate (CAP). Luteinising hormone release is inhibited by CAP, which is reflected in an increase of luteinising hormone cells in their storage form. This leads to statistically significant decline in androgenic testicular tissue percentage and in the cell nucleus volumes of Leydig's cells. Excessive weight decrease of the testicle is caused primarily by some 60 per cent reduction in tube length. The neurons of the tested hypothalamic nuclear region, that is the nucleus praeopticus medialis, have shown that in treated animals cell nucleus volumes were significantly depressed.

Animals↗

Boar proacrosin. Purification and preliminary activation studies of proacrosin isolated from ejaculated boar sperm.

Two forms of proacrosin have been purified from ejacualted boar spermatozoa. The isolation method utilized benzamidine to inhibit the premeture activation of the zymogen and included pH precipitation, ammonium sulfate fractionation, and sodium chloride precipitation. Further purification was achieved by Sephadex G-200 FILTRATION OF THE PREPARATION AFTER IT WAS TREATED WITH 8 M urea. The overall proacrosin yield was 58% with a specific acitivity of 253 units/mg of protein. The molecular weights of the proacrosins determined by sodium dodecyl sulfate disc gel electrophoresis were 55,000 and 53,000. Proacrosin autoactivation followed the classical S-shaped activation curve and calcium was not required to obtain full activation. Time course activation studies in 0.1 M Tris/HCl, pH 8.4, at 0 degrees were monitored with sodium dodecyl sulfate-disc gel eletrophoresis and anlytical gel electrophoresis with staining techniques for protein and enzymatic activity. Under the conditions used, the zymogens were sequentially degraded to three different active specise of acrosin (alpha, beta, and gamma). The approximate molecualr weights of the acrosins were 49,000, 39,000, and 25,000 for the alpha, beta, and gamma forms, respectively. The autoactivation is concentration-dependent and can be proteolytically stimulated with either alpha- or beta-acrosin and trypsin, indicating the activation of proacrosin can via a bimolecular process.

Acrosin↗