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PubMed2001

High-sucrose diet reduces defensive reactions of the pulpo-dentinal complex to dentinal caries in young rats.

The significance of systemic dietary effects on the response of the pulpo-dentinal complex to dentinal caries was examined. Weanling rats were divided into high sucrose or control diet groups both with and without cariogenic bacterial inoculation. At the onset, tetracycline was injected to mark the dentin formation during the experiment. After 5-6 week, mandibular molars were sectioned sagittally. The areas of dentin formed during the experiment and those of dentinal caries were quantified separately in the first and second molars. In the control diet groups the area of dentin was significantly greater under carious fissures, whereas in the high sucrose diet groups the area of dentin formed did not differ between intact and carious fissures. The high sucrose diet resulted in a significantly smaller area of dentin formation than did the control diet. The high sucrose diet with cariogenic bacterial inoculation resulted in the greatest area of dentinal caries. With the control diet a positive response against dentinal caries occurs, but the high dietary sucrose content impairs the defensive reactions of pulpo-dentinal complex against dentinal caries. These findings add further evidence of the importance of the local endogenous factors of caries progression.

Analysis of Variance↗

PubMed1990

Scanning electron microscopic observations of microcanals and continuous zones of interglobular dentin in human deciduous incisal dentin.

Scanning electron microscopic (SEM) observation of the coronal dentin of deciduous incisors revealed microcanals and continuous zones of interglobular dentin (CID) in the labio-lingual central portion of dentin beneath the incisal edge. The microcanals, which were clearly larger than dentinal tubules, extended from the incisal edge to the vicinity of the pulp cavity. They were located essentially in the labio-lingual central portion of the dentin and were arranged linearly in the mesio-distal direction. Inside the microcanals, collagen fiber bundles were arranged almost in parallel with the canal long axis: spherical bodies 1.0-2.5 microns in size made up of assemblies of regular parallelpipedal crystals and granulated crystals were also seen. In some instances, bacteria had invaded incisal dentin that had been exposed by attrition. The CID were made up of interglobular dentin aligned with the long axis of the tooth. As was true in the case of the microcanals, several zones were arranged irregularly in the mesio-distal direction, generally in the labio-lingual central portion of the coronal dentin. The CID were confined to about half the width of the incisal edge dentin on the incisal edge side and did not appear in the vicinity of the pulp cavity. Within the interglobular dentin itself, which was surrounded by calcospherites, were longitudinal collagen fibers connected with dentinal tubule walls inside calcified dentin and collagen bundles forming a network with those fibers. Spherical bodies 1.0-1.5 microns in size were observed attached to the surfaces of the bundles or distributed among these fibers. In some instances, the microcanals penetrated the CID.

Child↗

PubMed1995

Impregnation by dentine bonding agents into instrumented root-face dentine.

OBJECTIVES: To examine the penetration of unfilled resin into instrumented root-face dentine, facilitated by five commercially available dentine bonding agents. METHODS: Upper third molar teeth were decoronated, and a given dentine bonding agent and resin composite applied to the instrumented root face, according to the manufacturer's instructions. A control specimen was similarly prepared but not dentine primer was applied prior to resin composite. The teeth were sectioned longitudinally and the sectioned surface demineralized and deproteinated. Specimens were examined by scanning electron microscopy (SEM) for resin tag length and density in different areas of the root face. RESULTS: Specimens treated with dentine bonding agent systems exhibited minimum resin penetration of peripheral root-face dentine and much longer penetration of resin tags in dentine above the pulp chamber. A possible 'hybrid' layer of resin-impregnated dentine was observed in all specimens where a dentine bonding agent system had been used but was not observed in control specimens. Resin tags were also more sparsely observed in control specimens. CONCLUSION: Resin tag penetration is superior in both density and length of penetration in dentine overlying and adjacent to the pulp chamber, with shorter and sparser penetration exhibited in peripheral root-face dentine. The possible clinical implications of these observations are discussed.

Adhesiveness↗

PubMed2002

Wet or dry, normal or deproteinized dentin surfaces as substrate for dentin adhesives.

Bond strength between resin composite and dentin mediated by several dentin adhesives applied to dry or wet acid-etched dentin or to dry or wet acid-etched and deproteinized dentin were measured and analyzed. Human dentin were A) acid-etched and blot-dried for 1 sec (= wet), B) as A but dried with air for 10 sec (= dry), C) acid-etched and treated with hypochlorite and then dried for 1 sec, and D) as C but dried with air for 10 sec. Eight dentin adhesives were used in each group for bond strength measurements. The results were compared by means of ANOVA and the Tukey test. Collagen removal increased significantly the strength of the bond by 10-18 MPa for five adhesives when tested dry (D versus A). When tested wet, collagen removal increased the strength by 10-12 MPa for three adhesives (C versus A). Normal etched dentin showed a reduction in strength of 14-15 MPa for three or the adhesives when tested dry instead of wet (B versus A). For one dentin adhesive no significant change in bond strength due to collagen removal and/or drying conditions was observed. It was hypothesized that low technique sensitivity of an adhesive may be linked to its ability to wet and adhere to collapsed collagen fibers and to the surface of the underlying mineralized tissue. Comparisons of bond strengths obtained by using dried or wet acid-etched dentin and dried or wet acid-etched and deproteinized dentin may be useful for evaluating the efficiencies or dentin adhesives.

Acid Etching, Dental↗

PubMed2000

Bond strength of resin to acid-etched dentin studied by 13C NMR: interaction between N-methacryloyl-omega-amino acid primer and dentinal collagen.

The application of the hydrophilic methacrylate primer to acid-etched dentin increases the bond strength of the resin to the acid-etched dentin. However, the mechanism for the bonding of the resin to dentinal collagen through the primer remains to be determined. Before a more effective adhesive primer can be developed, we must understand the adsorption behavior of the primer to the dentinal collagen. The purpose of this study was to determine how 5 derivatives of N-methacryloyl-omega-amino acid (NMomegaA) primers enhance the bonding of the resin to acid-etched dentin. The interaction between the NMomegaA primers and dentinal collagen was studied by the 13C NMR technique, including the observation of spin-lattice relaxation times, T1. When the dentinal collagen was dispersed into the NMomegaA solution, the T1 values of the two carbonyl carbons attributed to the amide and the carboxylic acid in the NMomegaA molecule decreased dramatically. This result was due to the interaction between the amide group and the carboxylic acid group in the NMomegaA molecule and the dentinal collagen molecule. The T1 values of these carbonyl carbons decreased when the number of methylene groups in the NMomegaA molecule increased. The interaction became stronger as the number of methylene groups in the NMomegaA molecule was increased. Further, the bond strength of the resin to the acid-etched dentin primed with NMomegaA increased with a decrease in the T1 value of the amide carbonyl carbon. The strength of the interaction of the NMomegaA primer to the dentinal collagen molecule, determined by the 13C NMR technique, showed a direct correlation with the bond strength of the resin to acid-etched dentin that was treated with the NMomegaA primer.

Acid Etching, Dental↗

PubMed1997

Shear bond strength of four dentine bonding systems to dry and moist dentine.

Recent studies demonstrated that dentine wetness may be an important factor influencing the bond strength of dentine bonding systems. The purpose of this in vitro study was to evaluate the ability of four different dentine bonding systems to bond to dry (Group 1) and moist (Group 2) dentine. The four systems tested were Scotchbond Multi-Purpose (SMP, 3M), Imperva Bond (IB, Shofu), All-Bond 2 (AB2, Bisco) and OptiBond (OB, Kerr). In Group 1 the products were applied to dentine surfaces which had been dried with compressed air, after the etchant was rinsed off. In Group 2, however, the dentine was wiped with a damp facial tissue to remove excess water from the surface after rinsing off the etchant. The bond strengths (MPa) for the different products to dry (D) and moist (M) dentine were as follows: SMP: D: 24.9 +/- 4.5; M: 18.9 +/- 4.4; IB: D: 19.2 +/- 3.6; M; 15.6 +/- 2.8; OB: D: 25.9 +/- 2.9; M: 24.5 +/- 4.5 and AB2: D: 16.3 +/- 2.6; M: 27.2 +/- 2.3. Data were statistically analysed using the Wilcoxon Sum of Rank as well as Kruskal-Wallis tests. SMP and IB showed statistically significant higher bond strengths to dry dentine than to moist dentine. OB demonstrated no significant difference in bond strength between dry and moist dentine. AB2, however, demonstrated significantly higher bond strengths to moist than to dry dentine.

Dental Bonding↗

PubMed1994

Morphological features of dentine and pulp related to dentine sensitivity.

This review considers those structural features of the pulp and dentine relevant to an understanding of dentine sensitivity. It does not discuss innervation, or microvasculature, which are covered in other contributions. The sensitivity of dentine is directly related to the size and patency of the dentinal tubules. Tubular occlusion by peritubular dentine deposition or the formation of other intratubular material would reduce the flow of fluid and diffusion of molecules through dentine. Irregular (reparative) secondary dentine would, because its tubules are not continuous with those of primary dentine, be expected to reduce permeability and fluid flow and decrease sensitivity. Regular secondary dentine would have little or no effect other than by increasing diffusion distances. The odontoblast layer is of limited permeability and could restrict the access of materials diffusing through the dentinal tubules to pulpal axons. Odontoblasts are not involved in the sensory process as special sensory receptors but may, by modifying the local ionic environment, alter the threshold of intradentinal nerves.

Animals↗

PubMed2000

Effect of smear layer and direction of dentinal tubules on osteoblast adhesion to human dentin tissue.

The purpose of this study was to investigate the effect of smear layer and direction of dentinal tubules on osteoblast adhesion to human dentin tissue in vitro. Dentin disks were made from human premolars extracted for orthodontic reasons. Dentin disks were cut either perpendicularly to the long axis of the tooth or at 45 degrees to the long axis of tooth. The smear layer was removed by 34% phosphoric acid gel from half of the dentin disk surface. Human osteoblast-like Saos-2 cells were grown in RPMI medium with 10% fetal bovine serum and 1% antibiotic/antimycotic cocktail under standard cell culture conditions. Cells were seeded into Nunc four-well culture plates at 1.5 x 10(5) cells per well with dentin disks in the bottom of each well. After 1 day in culture the dentin disks along with cells grown on their surface were examined with a scanning electron microscopy. Osteoblasts attached and spread on the dentin surface and formed a monolayer in the presence and absence of a smear layer. Cells spread over the dentinal tubules despite their direction. These results suggest that cell adhesion and spreading of osteoblasts is not influenced either by the existence of a smear layer or the direction of the dentinal tubules on the dentin surface.

Acid Etching, Dental↗

PubMed2002

Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen, and heat-killed microbial whole cells.

The antibacterial activity of chlorhexidine digluconate and iodine potassium iodide on Enterococcus faecalis A197A was tested in the presence of dentin, dentin matrix, dentin pretreated by EDTA and citric acid, collagen, and heat-killed cells of Enterococcus faecalis and Candida albicans. Medications were preincubated for 1 h with each of the potential inhibitors and tested for their antibacterial activity against E. faecalis, strain A197A. Surviving bacteria were sampled after 1 and 24 h of incubation. Dentin matrix and heat-killed microbial cells were the most effective inhibitors of chlorhexidine, whereas dentin pretreated by citric acid or EDTA showed only slight inhibition. Dentin and skin collagen showed some inhibition at 1 h but not after 24 h. Iodine potassium iodide was effectively inhibited by dentin, dentin matrix, and heat-killed microbial cells. Skin collagen and dentin pretreated by EDTA or by citric acid showed little or no inhibitory effect on iodine potassium iodide. Different components of dentin are responsible for the divergent patterns of inhibition of the antibacterial activity of chlorhexidine digluconate and iodine potassium iodide. Chemical treatment of dentin before applying the medication into the root canal may alter the antibacterial effect of the medication.

Candida albicans↗

PubMed1998

[Effect of dentin inorganic material during dentin bond].

OBJECTIVE: In order to investigated the effect of dentin inorganic material during dentin bond. METHODS: This study investigated the tensile bond strength (TBS) between 4-META/MMA-TBB resin and dentin treated with several dentin treating methods. RESULTS: When the dentin was treated with acid-salt pretreating solution system (APSS) consisting of 10% citric acid and 3% ferric chloride to remove smear layer, and then re-treated with NaOCl to resolve dentin collagen and reveal dentin inorganic material, TBS of the dentin was 5.93 MPa, and higher than that of control group (3.71 Mpa) (P < 0.05) whose dentin was treated with distilled water only. CONCLUSION: It was suggested that dentin inorganic material did play a proper role in dentin bond.

Acrylic Resins↗

PubMedDate not supplied

Relationship between dentine hydraulic conductance and the cytotoxicity of four dentine bonding resins in vitro.

OBJECTIVES: Dentine modifies pulpward diffusion of monomers leaching from restorative materials. Thus, remaining dentine thickness must be taken into account during in vitro cytotoxicity tests. This in vitro study was designed to determine the influence of dentine permeability on the outcome of a cytotoxicity test. METHODS: Dentine slices were made from 36 human third permanent molar teeth. The 36 dentine slices were divided into two groups according to their hydraulic conductance: high or low hydraulic conductance. The cytotoxicity of four dentine bonding agents of similar cytotoxicity was tested on dentine slices from each group. Four dilutions of the experimental culture medium were tested: undiluted, 1:2, 1:10 and 1:100. An analysis of variance was used to compare the cytotoxicity of the dentine bonding agents tested on high versus low hydraulic conductance. RESULTS: The cytotoxicity of the high hydraulic conductance (Lp) group was higher than that of low Lp group when tested with the undiluted test culture medium (p = 0.001). No difference was obtained with the 1:2, 1:10, 1:100 dilutions. CONCLUSIONS: Under the conditions of the study, the dentine bonding resins were more cytotoxic when applied onto dentine slices of high hydraulic conductance.

Analysis of Variance↗

PubMed2006

The combined occluding effects of fluoride-containing dentin desensitizer and Nd-Yag laser irradiation on human dentinal tubules: an in vitro study.

The purpose of the present study was to evaluate the combined occluding effects of fluoride-containing dentin desensitizer and neodymium:yttrium aluminum garnet (Nd-YAG) laser irradiation on human dentinal tubules. All six of the groups of dentin samples (A-F) included in this study received applications of fluoride-containing dentin desensitizer. Groups B, D, and F also received Nd-YAG laser irradiation. Groups A and B served as controls, to allow observations of the occluding effects on the dentinal tubules before and after Nd-YAG laser irradiation. Groups C and D were treated with 0.5 M vitamin C solution, whereas groups E and F underwent brushing with an electric toothbrush. Scanning electron microscopy (SEM) revealed that the fluoridated dentinal tubule-occluding agent (FDTOA) formed a fine crystalline deposit on the dentin surface. After soaking in 0.5 M vitamin C solution for 3 hours, the crystalline deposit of the FDTOA was completely dissolved. Furthermore, brushing of the teeth 3,600 times removed most of the occluding agent. When the application of FDTOA was combined with Nd-YAG laser irradiation, the dentin melted and then recrystallized. The occluding agent was thus 'burned into' the dentinal tubules, and could neither be dissolved by vitamin C solution nor removed by brushing. Therefore, we concluded that the FDTOA combined with Nd-YAG laser irradiation burns the occluding agent into the dentinal tubules, thereby resisting the effects of an acidic diet and brushing, and increasing the duration of the desensitizing effect.

Combined Modality Therapy↗

PubMed2002

Penetration of an antibacterial dentine-bonding system into demineralized human root dentine in vitro.

In this study, the penetration of three proprietary dentine-bonding agents (Prime & Bond 2.1, Single Bond, Liner Bond 2) and experimental dentine-bonding systems incorporating an antibacterial monomer, 12-methacryloyloxydodecylpyridinium bromide (MDPB), into artificial root caries lesions was evaluated, and the bactericidal activity of each material against Streptococcus mutans or Lactobacillus casei impregnated into demineralized dentine blocks was assessed. All of the commercial dentine-bonding agents were capable of penetrating into the artificial carious lesions to more than 150 microm. The depth of penetration of the experimental systems, which were based on Liner Bond 2, was not significantly different from that of their parent product. Liner Bond 2 primer exhibited the greatest bactericidal effects among the three proprietary dentine-bonding agents tested. Bactericidal activities of experimental primers containing MDPB were greater than those of any other products, and the application of 4% MDPB-containing primer resulted in complete killing of bacteria in demineralized dentine. The results indicate that the penetration of dentine-bonding agents into extensively demineralized root dentine is possible in vitro, and the experimental dentine-bonding systems containing the antibacterial monomer MDPB are capable of killing bacteria within demineralized dentine. This could be of benefit when managing root caries lesions.

Acetone↗

PubMed1994

In vitro bond strengths and SEM evaluation of dentin bonding systems to different dentin substrates.

In comparison to enamel, bonding to normal dentin is a greater challenge because of its organic constituents, fluid-filed tubules, and variations in intrinsic composition. Bonding to sclerotic dentin is even more difficult. To evaluate the shear bond strengths of four adhesive systems to dentin substrates with different levels of mineralization, 120 extracted human teeth were randomly assigned to three groups (n = 40). After mid-coronal dentin was exposed, groups of specimens were artificially hypermineralized by immersion in a remineralizing solution, demineralized by means of an acetic acid demineralizing solution, or stored in distilled water to model sclerotic, carious, and normal dentin, respectively. Resin composite was bonded to dentin by use of commercial adhesive systems. After the specimens were thermocycled, shear bond strengths were determined in an Instron universal testing machine. Dentin substrates and resin/dentin interfaces were examined by SEM. For each adhesive system, the mean shear bond strength to normal dentin was significantly higher than that to either of the other substrates. Shear bond strengths to hypermineralized dentin were significantly higher than those to demineralized dentin with all adhesives except Prisma Universal Bond 3.

Analysis of Variance↗

PubMed2006

Influence of dentin bonding agents and polymerization modes on the bond strength between translucent fiber posts and three dentin regions within a post space.

STATEMENT OF PROBLEM: Debonding is the most frequent failure encountered with translucent fiber posts and usually occurs along the post space dentin-adhesive interface. PURPOSE: The purpose of this study was to evaluate the effect of different dentin bonding agents and polymerization modes on the bond strength between translucent fiber posts and root dentin in different regions of the post space. MATERIAL AND METHODS: Forty maxillary canines with similar root lengths were selected, sectioned at the cemento-enamel junction, and the roots were endodontically treated. Following post space preparation, the roots were divided into 4 groups of 10 specimens each, and the post spaces were treated with 1 of 4 different dentin bonding agents: light-polymerized, single-bottle bonding agent Excite (Group EX); dual-polymerized, single-bottle bonding agent Excite DSC (Group EX-DSC); self-etching primer Clearfil Liner Bond 2V with a light-polymerized bonding agent, Bond A (Group CL-LC); or self-etching primer Clearfil Liner Bond 2V with a dual-polymerized bonding agent, Bond A+B (Group CL-DC). Translucent fiber posts (D.T. Light-Post), 2.2 mm in diameter, were luted (Panavia F) in each specimen after respective dentin bonding procedures. The roots were cut into 3-mm-thick sections, perpendicular to the long-axis in cervical, middle, and apical post space dentin. Push-out tests were performed with a universal testing machine at a crosshead speed of 0.5 mm/min, and bond strength values (MPa) were calculated by dividing the force at which bond failure occurred by the bonded area of the post. The data were analyzed with 1- and 2-way analysis of variance and Tukey multiple comparison tests (alpha=.05). Dentin adhesive bonding mechanisms in different regions of the post spaces were evaluated with a scanning electron microscope. RESULTS: The highest mean bond strength values were obtained for Group CL-LC (18.3 +/- 4.1 MPa). The dual-polymerized bonding agent resulted in significantly lower bond strength (P<.001) in combination with self-etching primer (Group CL-DC) (13.2 +/- 2.5 MPa). The light-polymerized and dual-polymerized single-bottle bonding agents provided similar bond strengths (12.7 +/- 5.0 for EX; 13.5 +/- 5.3 for EX-DSC). The regional bond strength values of single-bottle bonding agents were reduced significantly in apical post space dentin (P<.001). Self-etching primers did not demonstrate regional differences in post space dentin bonding and dense resin tags were apparent. CONCLUSION: Data suggests that the self-etching primer system used in this study was unaffected by the morphological variations in the post space dentin compared to the single-bottle bonding agents. Dual polymerization did not improve the bond strength values of the bonding agents tested.

Analysis of Variance↗

PubMed2002

A comparison of microtensile bond strengths of several dentin bonding systems to primary and permanent dentin.

OBJECTIVES: Limited information exists with regard to the adhesive ability of glass ionomer cements (GIC) and recently developed resin-based dentin bond systems to primary dentin. The aim of this study was to compare the microtensile bond strength of a conventional GIC (Fuji IX), a resin-modified GIC (Fuji II LC), and two resin-based dentin adhesives (Prime and Bond NT with NRC and Single Bond). The bonded interfaces were also observed using field emission electron microscopy(FE-SEM). METHODS: Microtensile bond test specimens were prepared on superficial dentin of primary and permanent molars. The specimens were bonded according to each manufacturer's instructions except for Prime and Bond NT/NRC which used Silux Plus resin composite instead of Dyract. Hour-glass shaped specimens were created (diameter of 1.2+/-0.02 mm) and stressed in tension at a crosshead speed of 1mm/min. Results were analyzed using two-way ANOVA and LSD test, fracture modes were analyzed using the Mann-Whitney U-test and Kruskall-Wallis test. Twelve specimens were prepared for each material on primary and permanent dentin. Samples were prepared in the same manner, then critical point dried, fractured and sputter-coated for the FE-SEM observations. RESULTS: Two-way ANOVA showed the overall bond strengths were greater for the permanent dentin compared with primary dentin. However, for individual material comparisons no differences among the bond strengths to primary and permanent dentin for Fuji IX (9.7, 12.2 MPa), Fuji II LC (16, 20.1 MPa), Prime & Bond NT/NRC (18.1, 21.6 MPa) and Single Bond (18.2, 21.6 MPa), were detected. However, Fuji IX bond strengths were significantly lower than the other systems tested when bonded to either primary or permanent dentin (p<0.05). Failure mode showed cohesive failure of GIC and mostly adhesive failure for the resin-based adhesives. The FE-SEM observations showed hybrid-like layer formation for the GIC materials and hybrid layer formation for the resin-based adhesives. SIGNIFICANCE: The materials tested would be suitable for bonding to either primary or permanent dentin, but the resin-modified GIC or resin-based systems are likely to provide a stronger bond than the conventional GIC, Fuji IX.

Acid Etching, Dental↗

PubMed2004

Density of dentinal tubules affects the tensile strength of root dentin.

OBJECTIVES: The aim of this study is to count the dentinal tubules in the coronal and middle-apical third of root dentin of teeth extracted due to the progression of periodontal disease, and to compare the Ultimate Tensile Strength (UTS) of the same areas. The research hypothesis was that root dentin areas with different densities of dentinal tubules would also show different UTS values. METHODS: From 10 caries free maxillary central, lateral incisors and canines, extracted for periodontal reasons from three patients, cylindrical specimens approximately 10 mm long were prepared parallel to the long axis of the root and then divided into two parts using a low speed diamond saw one from the coronal third of the root, (Group 1) and one from the middle-apical third of the root (Group 2). The density of the dentinal tubules of the specimens of the two groups was measured by means of a scanning electron microscope and the UTS of the specimens was measured by a microtensile test. One way ANOVA was used to assess the effect of specimen location (coronal specimens vs. middle-apical specimens) on UTS. The differences in the density of dentinal tubules between coronal and middle-apical specimens were also subjected to statistical analysis using one-way ANOVA. RESULTS: UTS values of middle-apical specimens were found to be significantly (p < 0.05) higher than those of coronal specimens. The results of the one-way analysis of variance showed that the number of dentinal tubules of the samples from the coronal part of the root groups was significantly higher than that of samples from the middle-apical part (p < 0.05). SIGNIFICANCE: These results suggest that high values of tensile strength of the dentin are associated with low densities of dentinal tubules and that apical areas of root dentin are more resistant to tension than coronal ones.

Analysis of Variance↗

PubMed2001

Influence of Carisolv for resin adhesion to sound human primary dentin and young permanent dentin.

OBJECTIVE: This study evaluated the influence of Carisolv (Medi Team) for resin adhesion to sound human primary and young permanent dentin. METHOD: The buccal surfaces of 64 primary molars and 74 premolars were used. Two adhesive systems and resin composites were used; SD: Super-Bond D Liner DUAL (Sun Medical) and Clearfil Photo Anterior(Kuraray), and FB: Imperva Fluorobond and Lite-Fil IIA (Shofu). Ten groups were prepared. Groups 1-5 were primary dentin and Groups 6-10 were permanent dentin. Groups 1 and 6: Carisolv applied and agitated for 3min, SD was used. Groups 2 and 7: etched with 10-3 solution (Sun Medical) for 10s, SD was used. Groups 3 and 8: treated with Carisolv and then etched, SD was used. Groups 4 and 9: treated with Carisolv, FB was used. Groups 5 and 10: FB was used. The microstructural effects of Carisolv, 10-3 solution and Carisolv plus 10-3 solution applied to dentin were evaluated by SEM. In addition, the microstructure of the resin-dentin interfaces of each group were studied using SEM. Shear bond strengths (SBS) were tested, and the failed surfaces were observed using SEM. Data was statistically analyzed using ANOVA with subsequent application of post hoc Duncan's new multiple range test at p<0.05. RESULTS: The effect of Carisolv on primary dentin was stronger than that to permanent dentin. The mean SBS (unit:MPa) of Groups 1-10 were: 5.6, 15.8, 7.6, 17.5, 13.5, 8.1, 16.2, 18.2, 31.4 and 15.5. The SBS of Group 9 (Carisolv treated permanent dentin) was significantly higher than those of other groups. There was no significant difference of SBS among Groups 2, 4, 5, 7, 8 and 10, Groups 3, 5 and 6, and Groups 1, 3 and 6. SIGNIFICANCE: Carisolv treatment before etching significantly decreased the SBS to primary dentin in SD groups, but significantly increased the SBS to permanent dentin in FB groups.

Acid Etching, Dental↗