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Results for “HEMAGGLUTINATION INHIBITION TESTS”

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PubMed1991

[New human polyomavirus; BK virus antibody levels in different age groups using the hemagglutination inhibition test].

In this study, 1123 human sera samples obtained from healthy persons belonging to various age groups were tested in order to detect the distribution of antibody levels against the new human polyomavirus, BK. All sera samples were tested by hemagglutination inhibition (HI) test and 881 (78.5%) of those were found to be positive and 242 (21.5%) were negative. The percentage of positivity was 65.3% between the ages of 1 to 5 years, and over 80% in the group aged 6 years or older. Furthermore, between 1 to 10 years of ages HI antibody titers were higher than the older age groups. Our results indicate that antibody to BK virus infections are remarkably frequent in our population and acquisition of BK virus appeared to be common in childhood.

Adolescent↗
PubMed1986

Comparison of immunofluorescence and hemagglutination inhibition tests and enzyme-linked immunosorbent assay for detection of serum antibody in rats infected with hemorrhagic fever with renal syndrome virus.

Serum antibodies against the B-1 strain of hemorrhagic fever with renal syndrome (HFRS) virus in wild and experimental rats were investigated by the indirect immunofluorescent antibody (IF) test, the hemagglutination inhibition (HI) test, and an enzyme-linked immunosorbent assay (ELISA). In the newly developed ELISA, monoclonal antibodies to the B-1 strain were applied as coating antibody. In sera from wild rats, the results obtained by the ELISA agreed quite well with those obtained by the HI test, but some serum samples that gave a negative reaction in the HI test gave a positive reaction in the IF test, although their IF titers were very low. In serum samples from experimental rats that had been kept in an animal house infected with HFRS virus, a group with high titers and a group with low or negative titers were clearly differentiated by all the tests.

Animals↗
PubMed1987

Detection of postvaccination mumps virus antibody by neutralization test, enzyme-linked immunosorbent assay and sensitive hemagglutination inhibition test.

A group of 251 children aged 2-3 years given live attenuated mumps virus vaccine PAVIVAC of Czechoslovak production were tested for antiparotitis antibody levels in pre- and postvaccination sera by neutralization test (NT), enzyme-linked immunosorbent assay (ELISA) and sensitive hemagglutination inhibition test, enhanced by heterologous antibody to human immunoglobulin G (E-HIT). The prevaccination findings were as follows: positive ELISA IgG titres, neutralization antibodies and hemagglutination inhibition antibodies were present in, respectively, 35%, 25.9% and 27.9% of the sera. Postvaccination seroconversions were evaluated in 159 susceptible vaccinees whose prevaccination sera had been negative by all three tests. The lowest seroconversion was detected by NT (74.2%), seroconversions by ELISA and E-HIT were appreciably higher (82.4% and 86.8%, respectively). The seven children showing a seroconversion by E-HIT but not by ELISA had a 4 fold increase of anti-mumps ELISA IgG antibodies as well, but the rise of antibody titres was at a level falling in the range below the positivity criterion for ELISA. The statistically evaluated detection rate for antibodies was significantly higher (significance test "t") by ELISA as compared with neutralization test. However, antibody levels (geometric mean titres) were 8-10 times lower in postvaccination sera than in convalescent sera of 30 children with mumps in all three tests.

Antibodies, Viral↗
PubMed1985

Simple hemagglutination inhibition test for the diagnosis of toxoplasmosis.

A simple hemagglutination inhibition (HI) test for the serological diagnosis of toxoplasmosis has been developed and evaluated. A total of 84 human and 120 mouse serum samples were tested by the newly developed HI test and compared with an immunoglobulin G-indirect fluorescent antibody test. Statistical analysis of serum titers obtained by using the HI test and the immunoglobulin G-indirect fluorescent antibody test showed a correlation coefficient of 0.89. The diagnostic efficacy of HI when compared with the immunoglobulin G-indirect fluorescent antibody diagnostic test results was 96.43% for human sera and 100% for mouse sera. The unique hemagglutination antigen, derived from Toxoplasma gondii (Rh strain) exotoxin, spontaneously binds with mouse or rat erythrocytes, causing the hemagglutination reaction. In this study, 2, 4, or 8 hemagglutinating units of T. gondii exotoxin was used with Swiss/Webster mouse erythrocytes as an indicator for the HI assay. The results indicate that 8 hemagglutinating units is optimal because this concentration has the least unexplained variability. T. gondii exotoxin was stable for at least 18 months at -70 degrees C. The Toxoplasma HI test we report in this paper is shown to be a fast, easy, highly specific, and sensitive test for the diagnosis of toxoplasmosis.

Animals↗
PubMed1969

Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation.

Standardized hemagglutination and hemagglutination-inhibition procedures are described and statistically evaluated for all animal viruses where applicable, except for rubella and the arbovirus group. The standardized tests employ a constant phosphate-buffered saline diluent and constant volumes of serum, antigen, and standardized erythrocyte suspension. The standardized hemagglutination test has a reproducibility of 84 to 96% with adenoviruses, rubeola, and the myxoviruses, and 78 to 93% with reoviruses; the standardized hemagglutination-inhibition test has a reproducibility of 95 to 100% with all viruses tested.

Adenoviridae↗
PubMed1976

Rubella antibody assay by the immunoperoxidase technique: comparison with the hemagglutination inhibition test for determination of immune status.

The rubella immune status of 128 young women was determined with two assays for rubella antibody, the immunoperoxidase technique and the hemagglutination inhibition test. Comparison of the results of these techniques showed agreement in 125 cases (110 positive and 15 negative). Positive results were obtained for one woman only by the immunoperoxidase assay and for two women only by the hemagglutination inhibition test; all three of these women had a minimal titer of rubella antibody of 1:16. The sensitivity of the immunoperoxidase test was comparable to that of the hemagglutination inhibition test, and the specificity of the former test was verified. The peroxidase-labeled antibody technique is easier to perform than the hemagglutination inhibition test and is less time-consuming since sera do not require prior treatment and results can be obtained in as little as 90 min.

Antibodies, Viral↗
PubMed1980

A simple technique for quantitative measurement of erythrocyte settling patterns in hemagglutination inhibition tests for immunologic hormone assay.

A simple technique for direct quantitative evaluation of hemagglutination or hemagglutination inhibition tests used in immunologic proteo-hormone assay systems is described. The test is performed in calibrated glass capillaries (internal diameter 2.5...4 mm) at horizontal position. After sedimentation the non-agglutinated erythrocytes form two fine parallel stripes along the bottom of the capillaries. The distance between the stripes depends on the amount of additional free antigen brought into the system. It can be measured by the slit of a micrometer screw or a corresponding equipped microscope and might be used as y-variable for drawing calibration curves. At well standardized test conditions and for selected concentration ranges coefficients of variation between 10 and 15% can be achieved. So quantitative determinations can be made without serial sample dilutions to be prepared, covering concentrations ranges between 1 to 10.

Chorionic Gonadotropin↗
PubMed1988

[Detection of seropositivity against rubella virus in women in the reproductive age and comparison of the rubella hemagglutination inhibition test and the ELISA-IgG test].

In this study Hemagglutination Inhibition (HI) and ELISA-IgG tests used for Rubella serology were compared in terms of specificity and sensitivity In addition, 100 sera samples taken from women in pregnancy age were tested for Rubella antibodies. The results indicated that the sensitivity of Rubella ELISA IgG test is 100% and the specificity is 97.4%. The seropositivity of women in pregnancy age was found to be 93% by Rubella ELISA-IgG test.

Adolescent↗
PubMedDate not supplied

[The use of hemagglutination inhibition tests and complement fixation tests for evaluation of immunity states and diagnosis of recent rubella infections].

Comparative HAI and CF study of German measles antibodies in 575 sera collected from subjects of different age gave positive HAI reactions in 87% of the cases and positive CF reactions in 55% of the cases. The greater sensitivity of HAI and the longer presence of these antibodies justifies the use of this test for investigating the state of immunity in German measles. The combined use of the two tests for the diagnosis of the disease is very useful especially when the first serum sample is collected some time after the onset when the increase in serum antibody titers can only be demonstrated by CF, as HAI antibodies reach a high titer soon after the onset.

Antibodies, Viral↗