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At least 55 records · Page 3Linked to original sources
PubMed1991

[A case of peritonitis carcinomatosa due to advanced gastric cancer effectively treated by intraperitoneal injection of carboplatin].

The patient was a 72-year-old woman who had advanced carcinoma of the stomach. She presented massive ascites due to peritonitis carcinomatosa. The cytology of ascites was class V and the CEA level of ascites was elevated. Since renal function was low, we injected 300 mg of carboplatin intraperitoneally 2 times in 8 weeks. The amount of ascites was significantly diminished and the CEA level of ascites was decreased. The result suggested the effectiveness of intraperitoneal injection of carboplatin for the therapy of peritoneal metastasis of gastric cancer.

Aged↗
PubMed1991

Intraperitoneal injections of nanogram CCK-8 doses inhibit feeding in rats.

The satiating effect of intraperitoneal injections of synthetic cholecystokinin octapeptide (CCK-8) was tested in non-deprived and 17 h-deprived male and female Sprague-Dawley rats that were maintained on pelleted diet and offered evaporated milk test meals. At the midpoint of the 12-h diurnal period, 40-160 ng/kg CCK-8 elicited a dose-related decrease in meal size. At midnight, CCK-8's threshold for inhibiting feeding was increased to 80-160 ng/kg in nondeprived and deprived rats, respectively. These doses are 25-50 times less than typically required to significantly decrease food intake after intraperitoneal injection. No CCK-8 dose stimulated nocturnal feeding and no consistent sex difference was observed in CCK-8's effect. Intraperitoneal injection of ng amounts of CCK-8 may mimic a paracrine action of endogenous gut cholecystokinin to signal satiety.

Animals↗
PubMed1992

[Conditioned aversion to an intraperitoneally injected odor CS in rats].

Two experiments were conducted to specify an CS property of intraperitoneally injected odor substance in conditioned odor aversion formation. In experiment 1, three groups of water-restricted rats received one trial of conditioning with intraperitoneal injections of orange-extract (CS) and LiCl(UCS). The CS-UCS intervals of experimental groups were 30 minutes (Group IE-30) and 120 minutes (Group IE-120). In the test trial, Group IE-30 rats showed aversion to the test solution (0.5% orange extract), whereas no significant difference was observed between the control group and Group IE-120. In experiment 2, three groups of rats received one or two or three conditioning trials (Groups E1, E2 and E3, respectively). The CS-UCS interval of all groups was 30 minutes. In the test trial, rats in Groups E2 and E3 consumed less amount of test solution (0.5% orange extract) than the control rats, whereas no significant difference was observed between Group E1 and the control group. These results suggest that the intraperitoneally injected odor substance have CS property on conditioned odor aversion.

Animals↗
PubMed1984

Changes in cerebrospinal fluid volume and cardiovascular functions induced by intraperitoneal injection of hypertonic glucose solution in rats.

The present experiment was undertaken to study the relationship between hemodynamic changes and intracranial hemorrhage induced by intraperitoneal injection of large doses of hypertonic glucose solution in rats. A fifty per cent glucose solution was intraperitoneally injected at a volume of 3.5 ml/100 g b.w. into Wistar rats anesthetized with urethanechloralose. The time span from the start of injection to death was expressed in terms of 100% corrected death time (100% DT) for each rat. Saline that was added to the cerebrospinal fluid in the brain ventricle disappeared gradually from 30% DT up to death. Blood colloid osmotic pressure was slightly elevated temporally 5 minutes (7% DT) after intraperitoneal injection of the glucose solution and returned to the former level 5 minutes later. With regard to hemodynamic changes, a decrease in blood pressure and heart rate began to occur between 5-10% DT. Thereafter, blood pressure and heart rate decreased significantly as compared with the pretreatment period, and the plasma levels of both epinephrine and norepinephrine showed sharply increasing curves as time elapsed after the intraperitoneal injection of the hypertonic glucose solution. It was suggested that the decrease in cerebrospinal fluid and the fall in blood pressure were related to the movement of water from the brain and the flow of body fluid into the hypertonic blood plasma which caused an enlargement of the ventricles due to brain reduction and a change in circulating blood volume. However, questions still remain concerning the mechanisms of the marked increase in plasma catecholamines and bleedings which occurred only in the subarachnoideal space and ventricles.

Animals↗
PubMed1992

Intraperitoneal injection of chloral hydrate causes intra-abdominal adhesions and unilateral testicular atrophy in golden Syrian hamsters.

We investigated the reason for the high mortality we had observed in hypophysectomized-orchidectomized Golden Syrian hamsters that were anesthetized with intraperitoneal (i.p.) injections of chloral hydrate (CH). Intact male Golden Syrian hamsters were injected intraperitoneally with 0.1cc/100g BW of a 35% solution of CH, a 35% solution of sodium chloride, or double-distilled water. Equal numbers of hamsters in each group were injected on the right or left side of the abdomen. Within 10 days, 35% of the CH-injected hamsters were dead or had to be euthanized. Autopsy revealed severe peritonitis and adynamic ileus. CH-injected hamsters that survived gained weight at a rate similar to that of the controls. All surviving hamsters were killed 18 days after the injections. Among the surviving CH-injected hamsters, 84.6% had intra-abdominal adhesions, 61.5% had unilateral testicular atrophy, and 53.8% had a yellowish necrotic mass in the epididymal fat pad (EFP). All the lesions occurred on the side that was injected. The atrophied testes had been rendered cryptorchid due to involvement with intra-abdominal adhesions. In the water-treated controls, there were no abnormalities; whereas, in the saline controls, 75% had a mass in the EFP. Histology of the EFP mass was similar in hamsters injected with CH or hypertonic saline and suggested a diagnosis of fat necrosis. The results suggest that the mortality, the intra-abdominal adhesions, and the unilateral cryptorchidism were caused by a single i.p. injection of CH, but the fat necrosis in the EFP was probably caused by high concentrations of salt. The results further suggest that high concentrations of CH should not be injected intraperitoneally for anesthesia in chronic studies, particularly of the male reproductive system.

Adipose Tissue↗
PubMed1987

Localization of the monoclonal antibody HMFG2 after intravenous and intraperitoneal injection into nude mice bearing subcutaneous and intraperitoneal human ovarian cancer xenografts.

Xenografts (s.c. and i.p.) of human ovarian cancer, shown to express the tumor associated antigen defined by the monoclonal antibody HMFG2, were used to investigate in vivo localization of the radioiodinated antibody after i.p. and i.v. injection. Following i.v. injection, maximum uptake (31.4 +/- 3.5%/g s.c. tumor) was seen in s.c. tumors at 48 h after injection. Tumor:normal tissue ratios increased with time to 240 h. Uptake by ascites and i.p. tumors was less, with a maximum of 10.0 +/- 8%/g ascites reached at 16 h and 11.4 +/- 3.2%/g i.p. tumor at 96 h. After i.p. injection uptake in s.c. xenografts was maximal (14.8 +/- 2.0%/g) at 20 h. For ascites, the i.p. route of administration resulted in high uptake (27.7 +/- 5.8%/g) early (2 h) with an ascites:normal tissue ratio of 69.3 and a specific antibody:nonspecific antibody ratio of 30.8. Except for data at one time point, uptake by i.p. solid tumor was similar to that seen for s.c. tumor. These data strongly suggest that large concentration advantages can be achieved in ascites cells by regional i.p. injection, but that i.p. solid tumor may rely on i.v. delivery of antibody before uptake.

Animals↗
PubMed1989

Induction of hemopoietic chimerism in the caprine fetus by intraperitoneal injection of fetal liver cells.

Intraperitoneal injection of allogeneic liver cells from 43-day-old male fetuses into normal 60-day female goat fetuses resulted in persistent hemopoietic chimerism in surviving recipients without clinical evidence of graft-versus-host disease. Transplantation of normal fetal liver cells into preimmunocompetent goat fetuses affected with beta-D-mannosidosis may provide an alternative strategy for evaluating hemopoietic stem cell transplantation in the treatment of human lysosomal storage diseases.

Animals↗
PubMed1987

IgM antibody production in mice intraperitoneally injected with zirconium oxychloride.

The effect of zirconium (Zr) on the humoral immune response was studied by measuring the level of IgM-plaque forming cells (IgM-PFC) against sheep red blood cells (SRBC) in the spleen of C57 BL mice intraperitoneally injected with zirconium oxychloride. Two experiments, a single injection of zirconium oxychloride of 1/5, 1/10, 1/50, and 1/100 of the LD50 for intraperitoneal injection and continuous injection of 1/20, 1/40, and 1/80 of the LD50 every other day for two or four weeks in mice, were carried out. In the case of a single injection zirconium oxychloride was intraperitoneally injected on days -1, 0, +1, +2, and +3 in relation to SRBC immunisation. The following conclusions may be drawn from this study: (1) Zr was shown to have an adjuvant like activity in relation to the humoral immune response, at least to IgM antibody production; (2) this effect was recognised not only with a single injection with Zr but also after continuous injection; (3) a single injection of Zr was more effective when the mice were treated with Zr 24 hours before or after SRBC immunisation; and (4) with regard to an injected dose of Zr, it was shown that a lower dose (1/50, 1/100 of the LD50 for a single injection and 1/40, 1/80 of the LD50 for continuous injection) led to a more enhanced level of IgM-PFC than a higher dose (1/5, 1/10 of the LD50 for a single injection, and 1/20 of the LD50 for continuous injection).

Animals↗
PubMed1992

Plasma exudation by activated plasma kallikrein after intraperitoneal injection of lambda-carrageenin or endotoxin in rats.

The experimental study was carried out to examine whether intraperitoneal injection of carrageenin or endotoxin activates plasma kallikrein-kinin system to induce plasma exudation in rats. Intraperitoneal injection of 2% lambda-carrageenin induced plasma exudation in the peritoneal cavity by activation of plasma prekallikrein. Intraperitoneal injection of endotoxin (3mg/kg) also resulted in intraperitoneal plasma exudation, but plasma kallikrein-kinin system did not seem to be involved.

Animals↗
PubMed1990

Lysosomal glycogen accumulation in rat liver and its in vivo kinetics after a single intraperitoneal injection of acarbose, an alpha-glucosidase inhibitor.

A single intraperitoneal injection of acarbose (400 mg/kg) into rats caused lysosomal accumulation of glycogen in the liver, mimicking the cytological characteristics of human glycogen storage disease type II (Pompe's disease). The animal model is therefore useful for studying the pathogenesis of the disease. In the present study, we applied this model to examine the lysosomal hydrolytic pathway of glycogen in vivo. To quantify the lysosomal glycogen, the lysosome-rich fraction was rapidly prepared from liver homogenate by agglutination in the presence of Ca2+. Then the fraction was treated with alpha-amylase in isotonic medium to remove cytosolic glycogen, followed by transfer to hypotonic conditions in the presence of Triton X-100 to destroy total glycogen. The amount of lysosomal glycogen was calculated from the difference between the glycogen levels measured before and after the treatment under hypotonic conditions, and then it was corrected based on measurements of the intactness (%) of lysosomes and the recovery (%) of the lysosomal marker enzyme (beta NAGase). We observed no measurable lysosomal glycogen in normal liver by this method, and this was confirmed by electron microscopy. After administration of acarbose, the lysosomal glycogen level increased to 2.5 mg/g liver within 2 days, and then decreased gradually at a rate of 0.4 mg/day/g. The accumulation of glycogen in the lysosomes at an initial velocity of 1.5 mg/day/g liver may be considered as the amount of glycogen that would normally be degraded by acid alpha-glucosidase. Therefore, assuming that the liver breaks down about 40 mg glycogen/day/g, we estimated that about 3% of the glycogen would be hydrolyzed by the lysosomal pathway.

Acarbose↗
PubMed1991

[Observation of enzyme histochemistry and ultrastructure of brain in kindling rats with epilepsy induced by intraperitoneal injection of coriaria lactone].

Kindling model of epilepsy induced by intraperitoneal injection of Coriaria lactone (CL) was used in the experiment. The dose of CL was 1.25 mg/kg. Thirty rats in various periods of kindling were killed and the materials of cerebral cortex, hippocampus and cerebellum were drawn. The enzyme activities of AchE, NADHD, CCO, LDH, SDH, AcP, ANAE and AkP of these areas were observed with enzyme histochemical techniques. In another three kindled rats, two blank control rats and two NS control rats, the ultrastructure of neurons in hippocampus were observed. The results of experiments showed an increased activity of enzymes related to saccharometabolism and energy metabolism, indicating that the metabolism of brain in rats was increased by repeated kindling seizures. The mechanism of kindling seizure induced by CL may be related to inhibitory effect of CL on AchE activity of brain. The degeneration damage of brain neurons in kindled rats may result from using CL for a long time.

Acetylcholinesterase↗
PubMed1969

Intraperitoneal injection of mice.

Steward, Ornellas, Beernink, and Northway (2) reported a 14% error in the placement of intraperitoneal injections of mice. They considered this error inherent in the technique and not simply correctable. We similarly found an error in the intraperitoneal injection of mice and attempted to identify the cause of error by varying such technical procedures as size of needle, site of penetration (through lower left versus lower right quadrant), investigator, angle of needle to the abdominal wall, and speed of injection. None of these technical modifications consistently eliminated or reduced the error of placement.

Animals↗
PubMed1987

Effects of intraperitoneal injection of lithium chloride on neurohypophyseal activity: implications for behavioral studies.

Intraperitoneal injections of lithium chloride (LiCl) were found to increase the activity of vasopressin-neurons and oxytocin-neurons as indexed by rises in plasma concentrations of vasopressin-associated neurophysin (VP-RNP) and oxytocin-associated neurophysin (OT-RNP). Plasma VP-RNP increased 12 and 4 times basal levels (greater than or equal to 20 fmol/ml) reaching values of 248 +/- 37 fmol/ml (3.0 mEq LiCl/kg body weight) and 89 +/- 10 fmol/ml (1.5 mEq LiCl/kg body weight) at 60 minutes. OT-RNP rose to 37-and 10-times basal levels (greater than or equal to 20 fmol/ml) with peak values of 749 +/- 100 fmol/ml and 188 +/- 48 fmol/ml ten minutes following injection of 3.0 or 1.5 mEq LiCl/kg body weight. Mean arterial pressure increased in response to lithium treatment by 31 +/- 6 mm Hg at 60 minutes in rats receiving 3.0 mEq LiCl/kg and by 22.5 +/- 5 mm Hg at 10 minutes in rats receiving 1.5 mEq LiCl/kg over pretreatment values (125 +/- 3 mm Hg). Heart rate decreased from a pretreatment value of 422 +/- 12 beats/min to 367 +/- 48 beats/min at 10 minutes and to 341 +/- 27 beats/min at 20 minutes for rats treated with the high and low dose of lithium, respectively. These findings suggest that the behavioral effects of LiCl could result from multiple mechanisms and involve its acute release of vasopressin and oxytocin. It is also possible that changes in cardiovascular function may act as cues when LiCl is used as an aversive stimulus.

Animals↗
PubMed1989

Micronucleus test with methyl methanesulfonate administered by intraperitoneal injection and oral gavage.

The effects of 2 routes of administration, intraperitoneal injection (i.p.) and oral gavage (p.o.), in the micronucleus test were evaluated using methyl methanesulfonate (MMS) and 2 strains of mice (MS/Ae and CD-1). A small-scale acute toxicity study and a pilot micronucleus experiment were carried out first. On the basis of the results obtained, a final micronucleus test was performed at doses of 20, 40, 80, and 160 mg/kg (i.p.) and 40, 80, 160, and 320 mg/kg (p.o.), with a 24-h sampling time. MMS induced micronucleated polychromatic erythrocytes (MNPCEs) in both routes in both mouse strains under the conditions used. At 40 and 80 mg/kg, MMS induced a higher number of MNPCEs by the i.p. route in both strains. A 160 mg/kg MMS dose induced higher numbers of MNPCEs by the p.o. route in MS/Ae mice. The route-related difference with MMS on the basis of mg/kg disappeared when the difference was determined on the basis of a ratio of the LD50. In practice, both i.p. and p.o. routes are acceptable as routes of administration in the micronucleus test using this chemical.

Administration, Oral↗
PubMed1985

Reduced sleep in cats after intraperitoneal injection of delta-sleep-inducing peptide (DSIP).

The effect of intraperitoneally injected delta-sleep-inducing peptide (DSIP) on sleep-wakefulness in cats was studied using EEG, EMG and EOG recording for 10 h following 30 nmol/kg DSIP or control saline i.p. injections. DSIP reduced the amount of sleep, specifically light slow-wave sleep and REM sleep, and REM sleep latency was increased. The results suggest that in cats with redundancy sleep DSIP increases wakefulness at the cost of light slow-wave sleep, and in addition it has a specific REM-reducing effect.

Animals↗
PubMed1990

[A kindling model of epilepsy induced by intraperitoneal injection of coriaria lactone in rats].

Fifty adult male Sprague-Dawley rats were divided into five groups. Intraperitoneal injections of various doses of coriaria lactone (CL, 0.75, 1.25, 1.75, and 2.0 mg/kg) and normal saline were given respectively per 2 days. The behavior of the rats was observed and the ECoG was recorded by telemetric method. The results of experiments show that a kindling model of epilepsy can be established by intraperitoneal injection of CL in rats. This chronic experimental model is of value for application because it is easily established and the rate of being kindled is relatively high, but the mortality is low, and the kindling effect can last well. The model can avert the pathological change caused by artificial injury to brain, so it is advantageous to the research on neurochemistry and ultrastructure. In our experimental condition, it is optimal to select 1.25 mg/kg or 1.75 mg/kg as the dosage of CL for establishing kindling model.

Animals↗