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PubMed1990

[DNA hybridization and identification of Leptospira serogroup icterohaemorrhagiae with Leptospira recombinant DNA probes].

Two recombinant DNA probes-PLIpso1 (15kb) and PLIEc34 (4kb), derived from Leptospira serovaricterohaemorrhagiae genomic libraries, were applied for the hybridization and identification of 13 strains of Leptospira in serogroup icterohaemorrhagiae. Difference in hybridization signal in combination with the banding pattern provide a good way for identification of serovars and strains. The recombinant DNA, specific to L. serogroup icterohaemorrhagiae, hybridized with a limited number of DNA fragments which had been digested by several restriction endonucleases. The less complex banding pattern and higher sensitivity facilitate characterization of various serovars and strains in serogroup icterohaemorrhagiae. In general the DNA patterns recognized by both probes have extensive genomic homology in same serovar (but still can distinguish strains in same serovar by some unique bands) and apparent difference in various serovars (especially serovars naam, nanxi and honghe). The results indicated that Southern blotting with recombinant DNA probe might provide tools for identification, characterization and analysis of leptospira.

Blotting, Southern↗
PubMed1981

The Norway rat as a selective chronic carrier of Leptospira icterohaemorrhagiae.

Laboratory raised wild Norway rat males (Rattus norvegicus) were injected with leptospires of two serovars: icterohaemorrhagiae and grippotyphosa. The development of a carrier state was monitored serologically, culturally and histologically. Rats infected with icterohaemorrhagiae developed rapidly into a chronic carrier state and shed leptospires in the urine for the duration of the experiment (220 days). At the time of necropsy, histopathologic studies showed evidence of leptospiral infections in the lumen of proximal convoluted tubules of some kidneys. Rats infected with grippotyphosa shed organisms for 40 days after inoculation; thereafter, they apparently cleared the infection. No organisms were detected histologically nor by culture at the end of the experiment (220 days). There appears to be a specific host-parasite relationship in the Norway rat towards becoming chronic carriers when infected with serotype icterohaemorrhagiae but not with grippotyphosa.

Animals↗