ANALYSIS OF CERTAIN VOLATILE OILS IN AEROSOL FORMULATIONS.
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OBJECTIVE: To study the different extraction methods of the volatile oil from Mosla soochowensis Matsuda and to determine the optimum extraction method. METHODS: The yields and the chemical constituents of volatile oil were used to compare the different extraction methods including SFE-CO2, steam distillation and organic solvent (petroleum ether) extraction. RESULTS: The yield of volatile oil by means of SFE-CO2 was 3.46%, which was higher than others. The quality of the oil by means of SFE-CO2 and steam distillation was better. Based on the result of GC-MS, the constituents of volatile oil were similar. It was mainly the methyleugenol, accounted for about 42%. The second was monocyclic terpene, such as bornene, dihydrocarvon, carvacrol, accounted for about 27%. The third was sesqutierpenoids, such as nerolidene etc, accounted for about 19%. CONCLUSION: SFEE-CO2 is the optimum extraction method of the volatile oil from Mosla soochowensis Matsuda.
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Volatile oils extracted by steam distillation from leaves of two plant species Moschosma polystachyum and Solanum xanthocarpum were evaluated in mosquito cages for their topical repellency effects against filarial vector Culex quinquefasciatus. The oil from M. polystachyum was tested at four different concentrations ranging from 1 to 4%. The 4% concentration gave 332.2 minutes protection whereas control gave only 4.4 minutes protection against mosquito bites. However, the volatile oil of S.xanthocarpum was tested from 2 to 8% concentration. The 8% concentration gave 311.4 minutes protection whereas control gave only 4.4 minutes protection against mosquito bites. The results suggest that the volatile oils of these two plant species were effective as repellents and gave more than 300 minutes of (>5 hour) protection against the bite of Cx. quinquefasciatus bite. Both volatile oils did not cause dermal irritation when applied to human skin. No adverse effects on human volunteers were observed after application. Therefore, both volatile oils can be applied as an effective personal protection measure against mosquito bites.
The effects of the volatile oil of Rosmarinus officinalis leaves on the tracheal smooth muscle of rabbit and guinea pig were tested in vitro using isolated tracheal strips. The volatile oil of R. officinalis leaves inhibited the contractions of rabbit tracheal smooth muscle induced by acetylcholine stimulation and the contractions of guinea pig tracheal smooth muscle induced by histamine stimulation. Also, the volatile oil inhibited the contractions of rabbit and guinea pig tracheal smooth muscle induced by high potassium (K+) solution. This inhibition was dose-dependent and reversible. Furthermore, the volatile oil inhibited the contractions of rabbit and guinea pig tracheal smooth muscle induced by acetylcholine and histamine stimulation, respectively, in Ca(2+)-free solution. These data suggest that the volatile oil of R. officinalis leaves has a calcium antagonistic property.
The volatile oil from the roots of Patrinia scabra Bunge was isolated by steam distillation, and separated into four major fractions (Fr. A-D) by means of column chromatography. A total of 39 compounds (1-39) were identified by GC/MS analysis, and evaluated for their in vitro cytotoxic activities against human ovarian carcinoma cells (HO-8910) and human hepatoma cells (Bel-7402) (Table 1). Fr. A showed the strongest inhibitory effect on HO-8910 (IC50 = 21 microg/ml) and Bel-7402 cells (16 mcirog/ml), whereas Fr. B was the least active (>100 microg/ml). By comparison of the constituents of the four fractions, we assume that the cytotoxicity of the volatile oil of P. scabra is mainly due to the lignans and azulenes, rather than to caryophyllene oxide I (18). Our results suggest that the volatile oil of P. scabra possesses potent and tumor-specific cytotoxicity, and could serve as a possible candidate for future cancer chemotherapy.
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OBJECTIVE: To study optimum inclusion process conditions for volatile oil from Rhizoma Curcumae. METHOD: The study was carried out with orthogonal design. The process conditions were studied by determining the utilazision ratio of volatile oil from Rhizoma Curcumae, the oil-bearing rate and extract ratio of inclusion compound. RESULTS: The optimum preparation conditions for inclusion were established as: volatile oil:beta-CD was 1:8, the inclusion temperature was at 80 degrees C and inclusion time for 3 h, ultilizasion rate of volatile oil was 86%. CONCLUSION: The method can be used for mass production.
Rumen microorganisms of wild and captive deer were subjected to increasing amounts of volatile oils. The oils had a marked antibacterial effect on the rumen bacteria when the concentration reached approximately 16 muliters of oil per 10 ml of rumen fluid nutrient broth. The gross reactions of rumen bacteria obtained from wild, as well as captive, deer to the volatile oils seemed to be of the same magnitude; thus no adaptation by the bacteria to the oils was apparent.
OBJECTIVE: To analyse chemical constituents of volatile oil in Armeniaca mume Sieb. METHODS: After stream distillation, the volatile oil was extracted with ether and concentrated. Then it was analysed by gas chromatography/mass spectrometry. RESULT: More than 70 different compounds were separated and identified from the volatile oil in A. mume. The identified constituents from each kind of A. mume represent about 90% of the total volatile oil. CONCLUSION: The main compounds of the volatile oil in A. mume were hexadecanoic acid, linoleic acid, benzoic acid, furfural, benzaldehyde, benzenemethanol, etc.