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PubMedDate not supplied

[Establishing the time of death by the putrefactive transformation of the corpse].

Electron paramagnetic resonance (EPR) was used for assessing the time of death. The kinetics of the reaction of the rate of recovery of spin probes incubated with homogenized muscle tissue was studied by repeatedly recording the central component of the probe followed by measuring its amplitude. Muscle tissue of 35 corpses was studied 7 to 182 days postmortem with a one-week interval. The results indicate that EPR may help assess the time of death in corpses transformed by putrefaction. The amplitude of the central component of spin probe EPR spectrum is recommended as the criterion of assessing the length of postmortem period.

Adolescent↗
PubMedDate not supplied

[Putrefaction-induced green discoloration of cadaver skin with circumscribed sparing of areas in contact with tape strips in suicidal asphyxia].

A 67-year-old woman committed suicide by smothering her head tightly in gummed tape. When the body was found, a putrefactive greenish discoloration had spread across the neck, chest and abdomen; but the face was spared. The pale face and the greenish trunk caused a striking contrast. This distribution pattern of discoloration was produced by the gummed tape, which smothered the head and prevented the formation of sulfhemoglobin by blocking entry of ambient oxygen into the skin.

Aged↗
PubMed1985

[Immunoenzyme demonstration of isoantigens A, B and H in kidney tissue changed by putrefaction].

In contrast to blood serology, which usually fails in specimens more than a few days old, immunohistochemistry (PAP technique) provided reliable information on the blood group (ABO) and, in most cases, also the secretor character of 23 kidney specimens stored for months at room temperature. Better results were obtained with monoclonal antibodies than with human sera. In the late stages of decomposition, blood group diagnosis is based on the more decomposition-resistant antigens of the collecting tubular epithelium (in secretors) and the endothelia of the arteriolae medullares rectae and not on the identification of erythrocytic antigens. In addition, a decomposition-resistant epithelial antigen in the distal convoluted tubules (Tc II) is unmasked by autolysis or heterolysis. "Blood group" antigens were frequently detected in bacteria and fungi. These antigens, however, were clearly distinguishable from blood group characters of the tissue. A transient, weak, false-positive reaction with monoclonal anti-B appeared in decomposed Tc II epithelia.

ABO Blood-Group System↗
PubMed1986

[Putrefaction-induced changes in the concentration and temporal detectibility of carbon monoxide in cadaveric blood samples].

Eighty-five blood samples with COHb concentrations of 40% and 70% were allowed to putrefy in order to measure the time-dependent changes in COHb values. The samples not hermetically closed had been stored at 6 degrees C and 20 degrees C. GC and photometric methods were applied. Two graphs show that after 15 days (40% COHb) and after 30 days (70% COHb), the COHb concentrations decreased to half at 20 degrees C. Corresponding decreases at a storage at 6 degrees C could be observed after 1 year. Further investigations excluded the formation of new CO.

Carbon Monoxide Poisoning↗
PubMed1984

[Significance of the putrefactive bacterium Clostridium sordellii for the determination of age of the cadaver].

The method of determining postmortal interval by means of the content of ABU, GABA and GLU in putrefied postmortem brain is based on the speed of spreading, the metabolism and the growth of certain clostridia. The experiments have shown that the fecal bacteria can reach the brain in a few days. The significance of this temperature-dependent invasion ability of the bacteria for the determination of the age of the corpse is discussed.

Amino Acids↗
PubMedDate not supplied

The effect of conditions of putrefaction on species determination in human and animal teeth.

Tooth fragments freshly extracted from humans and rats were stored at either 4 degrees C or room temperature in dry or humid conditions for periods ranging from 1 to 6 months. The fragments were reduced to powder and antigens were extracted. Comparison of these samples was carried out using Counter Current Electrophoresis. Extracted sera were tested against known specific antisera and resultant precipitin reactions stained for examination. Correct species identification was possible both from desiccated and humid fragments but there was species variation in the sensitivity of the method. All the extracts from human teeth were positive against human antisera. In the rat some test specimens were initially negative but became positive following further dilution of the extracts.

Animals↗
PubMed1994

The effects of putrefaction on haptoglobin phenotyping from tooth fragments.

Tooth fragments freshly extracted from humans were stored for various times and temperatures under both dry and moist conditions. The fragments were reduced to powder and proteins eluted. Purified haptoglobin fractions were separated, detected and phenotyped using the Phast Gel electrophoresis system using gradient gels. Haptoglobin phenotypes were demonstrable but became less detectable as time increased.

Biomarkers↗