Reproduction of the normal sexual cycle and reproductive ability in castrated guinea pigs by implantation of the ovary by a new method.
Hormonal and generative activity of the "free" ovarian graft in the uterine wall was studied in 43 sexually mature guinea pigs. Ovaries were grafted by a personal method and by the method of Bland and Donovan. In the author's method, halves of the ovaries were implanted in the uterine wall in such a way that the graft communicated with the uterine cavity and received supplementary vascularization from the connective tissue of the parametrium. Morphologic and histochemical (PAS-positive substances) changes in the free ovarian grafts in the uterus were studied. Hormonal activity of grafts was assessed on the basis of vaginal smears. It was concluded that hormonal activity of the ovary engrafted in the uterine wall depends on the method of grafting. By the author's method, in which blood vessels from the parametrial connective tissue penetrate into the ovary, hormonal activity is the same as before operation and pregnancy is possible. In the method of Bland and Donovan, the sexual cycle and time of opening of the vagina are considerably shortened.