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At least 55 records · Page 3Linked to original sources

PubMed1977

Prolonged sarcotubular regenerative response in frog sartorius muscle.

The sarcotubular system of frog sartorius muscle fibers is found to generate a prolonged depolarizing regenerative response under appropriate experimental conditions. It is shown from a point-voltage clamp analysis that the inward current component responsible for the response lasts on depolarization for hundreds of milliseconds, and reverses its sign when an equilibrium potential is exceeded. The sarcotubular response occurs in F-Ringer solution and is enhanced with EDTA. It also occurs in normal or propionate-Ringer solution containing EDTA without great change in the equilibrium potential. The response is inhibited reversibly with picrotoxin. Partial replacement of Na by K in the propionate-Ringer solution reduces the inward current component which suggests that Na may be the responsible ions.

Animals↗

PubMedDate not supplied

Sartorius muscle coverage for the treatment of complicated vascular surgical wounds.

Sartorius muscle flaps were evaluated in 14 patients treated for complicated groin wounds related to vascular surgical procedures. Particular risk factors for poor wound healing were present in 8 patients, including diabetes, obesity, or poor nutrition in 3 patients; prior radiotherapy to the groin in 2; reoperative groin exposure in 3. Eleven of 14 patients had multiple indications for sartorius flap coverage, including hemorrhage in 5 patients, groin wound sepsis in 3, graft sepsis in 2, wound necrosis in 5, exposure of a reconstruction in 4, treatment of a lymphocele in 1, and treatment of groin irradiation in 2. Nine flaps were proximal sartorius segments rotated into the groin, four were distal muscle reflected into the groin, and one was a mobilized mid-portion of the muscle. Early successful wound healing occurred in all patients, but complications included muscle bed infection in 1 patient, late recurrence of lymphocele in 1, and recurrent groin sepsis in 1. Hemorrhage did not recur, and existing reconstructions were salvaged in all patients. The sartorius flap is a well-tolerated method for achieving autogenous tissue coverage for the problem groin wound, and there is no long-term functional deficit from anatomic loss of the sartorius muscle.

Groin↗

PubMed1985

The effects of pH on the kinetics of fatigue and recovery in frog sartorius muscle.

The effects of pH on the kinetics of fatigue and recovery in frog sartorius muscle were studied to establish whether the pH to which muscles are exposed (extracellular pH) has an effect on both the rate of fatigue development and recovery from fatigue. When frog sartorius muscles were stimulated with short tetanic stimuli at rates varying from 0.2 to 2.0 trains/s, a time- and frequency-dependent decrease in force development was observed, but extracellular pH had comparatively little effect. The recovery of tetanic force was dependent on the extracellular pH. This effect was characterized by a rapid recovery in force at pH 8.0 and an inhibition of recovery at pH 6.4 even when force decreased by only 25% during stimulation. Even when muscles were fatigued at pH 8.0 the rate of force recovery was still very small at pH 6.4. A model is proposed in which a step of the contraction cycle changes from a normal to a fatigued state. The rate of this transition is a function of the stimulation frequency and not pH. The reverse transition, from a fatigued to normal state is pH dependent; i.e., it is inhibited by H+. Measurements of resting and action potentials show that extracellular pH influences these parameters in the fatigue state, but there is no evidence that these changes are directly responsible for the pH-dependent step in the reversal of fatigue.

Action Potentials↗

PubMed1995

Anomaly of the femoral artery passage through the substance of sartorius muscle. Clinical consequences.

The passage of the femoral artery through the substance of the sartorius muscle and its clinical consequences are presented. In the series of cadavers studied, the incidence of occurrence of this condition is probably less than 0.001% in both Caucasians and black Africans. It is suggested that this anomaly which rarely occurs is due to the angioblastic column of cells forming the femoral artery passing through the myoblastic column of cells forming the sartorius muscle.

Black People↗

PubMed1988

A microelectrode study of the mechanisms of L-lactate entry into and release from frog sartorius muscle.

1. Changes in intracellular pH and intracellular anion levels were monitored in frog sartorius muscle fibres during exposure to extracellular L-lactate, using ion-sensitive microelectrodes. 2. Resting intracellular pH (pHi) in 20 mmol l-1 HEPES buffer was 7.18 +/- 0.015 (S.E. of mean, n = 62). Exposure to an extracellular solution at pH 6.5 buffered with 20 mmol l-1 3-(N-morpholino)propanesulphonic acid (MOPS) resulted in a slow intracellular acidification. 3. A reversible decrease in pHi and an increase in intracellular anion levels was observed when L-lactate replaced chloride in equimolar amounts. The increase in intracellular anion level is consistent with intracellular accumulation of L-lactate ion. 4. The rate and steady-state change in pHi and anion level was a function of both extracellular pH and L-lactate concentration, providing evidence for the coupled movement of lactate and proton equivalents. 5. The initial rate of uptake of L-lactate, as measured by the change of pHi, was a non-linear function of the extracellular L-lactate concentration at extracellular pH 6.8 and 7.35. 6. No saturation was observed with concentrations of L-lactate between 5 and 60 mmol l-1 at pH 7.35 and 2.5 and 40 mmol l-1 at pH 6.8. 7. The non-linear relationship between the initial rate of change in pHi and extracellular L-lactate was well fitted by a curve defining uptake as the sum of a carrier process displaying Michaelis-Menten kinetics and a passive diffusion component. The apparent Km of the carrier was 10 mmol l-1 at pHo 7.35 and 4 mmol l-1 at pHo 6.8. 8. The initial rate of change of pHi in the presence of L-lactate was significantly inhibited 39.1 +/- 6.2% by 2-5 mmol l-1 alpha-cyano-4-hydroxycinnamate (n = 9; P less than 0.05, paired t test). 9. alpha-Cyano-4-hydroxycinnamate had no detectable effect on the initial rate of change of pHi induced by propionate exposure. 10. The initial rate of change of pHi induced by L-lactate was not affected by 20-100 mumol l-1 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (SITS). 11. We conclude that L-lactate crosses the membrane of the frog sartorius muscle with proton equivalents via (1) a carrier-mediated process, and (2) passive diffusion of lactic acid. In the physiological range of L-lactate concentrations and pH the transport process dominates.

Animals↗

PubMed1989

The use of sartorius muscle rotation-transfer in the management of wound complications after infrainguinal vein bypass: a report of eight cases and description of the technique.

Although rare, major wound breakdown after infrainguinal bypass resulting in vein graft exposure carries the risk of vein graft disruption with threat to both life and limb. The use of sartorius muscle rotation-transfer specifically in the management of exposed autogenous infrainguinal vein grafts has not been previously described. Eight patients were evaluated for major wound disruption resulting in graft exposure after infrainguinal vein bypass. Soft tissue coverage was provided in all eight cases by means of a distally based sartorius muscle rotation flap. There was no instance of postoperative death, graft thrombosis, secondary hemorrhage, or persistent infection. Late follow-up has shown continued satisfactory results. We conclude that effective soft tissue coverage and salvage of exposed infrainguinal vein bypass grafts can be accomplished in selected cases by means of sartorius muscle rotation-transfer.

Adult↗

PubMed2006

Mechanism of N-ethylmaleimide-induced contraction of the frog sartorius muscle.

We studied parameters of the frog sartorius muscle contraction initiated by ryanodine receptor agonists in the presence of ROS donors. We hypothesized that sodium nitroprusside and hydrogen peroxide inhibit initiation of contractions by N-ethylmaleimide and that this effect of ROS donors on parameters of N-ethylmaleimide-induced contractions is due to a direct effects of sodium nitroprusside and hydrogen peroxide on N-ethylmaleimide, but not to inactivation of ryanodine receptors in the sarcoplasmatic reticulum of frog skeletal muscle.

Animals↗

PubMed1980

Effect of length and caffeine on isometric tetanus relaxation of frog sartorius muscles.

In an isometric tetanus of frog sartorius muscle the total relaxation time increased linearly with change in length from 0.7 to 1.4 times rest length. Maximal rate of relaxation, measured from the time derivative (dp/dt) of tension decay, decreased with both decrease and increase from rest length in correlation with the generated tetanus tension. Stretching the muscle did not significantly affect the times to maximal rate, positive and negative inflexion points but greatly increased the time to total relaxation from the negative inflexion point. Caffeine at 2 mM, acting on muscles at rest length, also slowed the relaxation and decreased the maximal rate of tension decay. However, caffeine increased the times to maximal rate, positive and negative inflexion points without significantly affecting time to total relaxation from the negative inflexion point. These results suggest that caffeine slows an earlier step in relaxation, while stretch slows a later step. It is proposed that muscle relaxation is a two step process: an initial step that is regulated by the rate of Ca2+ uptake by sarcoplasmic reticulum, and a later step that is mostly controlled by the speed of dissociation of remaining cross-bridges.

Animals↗

PubMedDate not supplied

Early transposition of the sartorius muscle for exposed patent infrainguinal bypass grafts.

The traditional approach for patent and exposed and infected infrainguinal bypass grafts in the groin has included wide operative debridement and secondary or delayed primary closure. However, this has been associated with significant risk of further contamination and length of stay. The authors reviewed their experience using the wide debridement, sartorius muscle flap transposition, and primary wound closure as an alternative. During the past 5 years, they have had 50 patients with major wound necrosis or infection in the groin or thigh with the graft or native artery being exposed after debridement. This group included 28 men; 74% of the patients had hypertension, 58% had diabetes, and 20% had renal failure. The grafts were split evenly between native vein and prosthetic material. After wide debridement, closure was performed by the vascular surgeon using the sartorius muscle flap. Postoperatively, there was an 8% major amputation rate and a 12% mortality rate in the first 30 days. One patient developed a pseudoaneurysm 5 weeks after placement of the flap. This patient underwent removal of the infected polytetrafluoroethylene graft with ligation of the common femoral artery. None of the procedures have resulted in further systemic or graft sepsis. None have resulted in arterial or graft blowout. Follow-up was for an average of 18 months. Closure of groin and thigh wounds with exposed bypass graft or native artery can be safely performed with the sartorius muscle flap with excellent results. The length of stay of these patients compared to historical controls is acceptable. Furthermore, the chance of infection of the native artery or bypass may be reduced. Familiarity with this simple technique can be a valuable tool for the vascular surgeon.

Amputation, Surgical↗

PubMed1992

Effects of K+ on the twitch and tetanic contraction in the sartorius muscle of the frog, Rana pipiens. Implication for fatigue in vivo.

The effects of increasing the extracellular K+ concentration on the capacity to generate action potentials and to contract were tested on unfatigued muscle fibers isolated from frog sartorius muscle. The goal of this study was to investigate further the role of K+ in muscle fatigue by testing whether an increased extracellular K+ concentration in unfatigued muscle fibers causes a decrease in force similar to the decrease observed during fatigue. Resting and action potentials were measured with conventional microelectrodes. Twitch and tetanic force was elicited by field stimulation. At pHo (extracellular pH) 7.8 and 3 mmol K+.L-1 (control), the mean resting potential was -86.6 +/- 1.7 mV (mean +/- SEM) and the mean overshoot of the action potential was 5.6 +/- 2.5 mV. An increased K+ concentration from 3 to 8.0 mmol.L-1 depolarized the sarcolemma to -72.2 +/- 1.4 mV, abolished the overshoot as the peak potential during an action potential was -12.0 +/- 3.9 mV, potentiated the twitch force by 48.0 +/- 5.7%, but did not affect the tetanic force (maximum force) and the ability to maintain a constant force during the plateau phase of a tetanus. An increase to 10 mmol K+.L-1 depolarized the sarcolemma to -70.1 +/- 1.7 mV and caused large decreases in twitch (31.6 +/- 26.1%) and tetanic (74.6 +/- 12.1%) force. Between 3 and 9 mmol K+.L-1, the effects of K+ at pHo 7.2 (a pHo mimicking the change in interstitial pH during fatigue) and 6.4 (a pHo known to inhibit force recovery following fatigue) on resting and action potentials as well as on the twitch and tetanic force were similar to those at pHo 7.8. Above 9 mmol K+.L-1 significant differences were found in the effect of K+ between pHo 7.8 and 7.2 or 6.4. In general, the decrease in peak action potential and twitch and tetanic force occurred at higher K+ concentrations as the pHo was more acidic. The results obtained in this study do not support the hypothesis that an accumulation of K+ at the surface of the sarcolemma is sufficiently large to suppress force development during fatigue. The possibility that the K+ concentration in the T tubules reaches the critical K+ concentration necessary to cause a failure of the excitation-contraction coupling mechanism is discussed.

Action Potentials↗

PubMed1996

Surgical transfer of the sartorius muscle to the groin after lymphadenectomy or debridement: CT findings.

OBJECTIVE: We describe the CT findings of medial transfer of a sartorius muscle flap, which is done to protect the femoral blood vessels after radical inguinal lymphadenectomy for cancer or surgical debridement of infected femoral vascular grafts. MATERIALS AND METHODS: We reviewed the appearances and initial interpretations of 33 CT studies in 17 patients who underwent medial sartorius flap transfer after either (1) inguinal lymphadenectomy for melanoma or penile cancer or (2) debridement of infected groin wounds complicating vascular reconstruction of the femoral arteries. Muscle flap transfer was defined by the surgical record as either complete or incomplete. In complete sartorius muscle transfer, the proximal end of the muscle is dissected from the anterior superior iliac spine, rotated along its long axis, and sutured medially to the inguinal ligament. In incomplete transfer, the muscle it mobilized and stretched medially, and its medial border is fixed to the inguinal ligament and deep tissues. Clinical correlation and follow-up examinations were done for all patients, and CT reevaluation at intervals was done in nine patients. RESULTS: Complete sartorius flap transfer resulted in a mass anterolateral or anterior to the femoral vessels on postoperative CT scans in 20 studies; five of these masses were misinterpreted initially as possible recurrent metastatic lymphadenopathy, infection, or hematoma. Incomplete sartorius flap transfer resulted in bandlike stretching of the muscle over the femoral vessels in 13 studies. CONCLUSION: Medial transfer of the sartorius muscle causes a variable appearance of the groin on CT scans. The findings on CT scans after complete sartorius flap transfer should be distinguished from recurrent lymphadenopathy and from postoperative phlegmon or hematoma.

Debridement↗

PubMed1983

Effects of step changes in pH on isometric tetanic tension of toad sartorius muscle.

The effect of a rapid change in pHe (pH of bathing solution) on the isometric tetanic tension developed by sartorius muscles of toads acclimated to 5 and 25 degrees C was measured at 5 and 25 degrees C. The pH was altered by changing the carbon dioxide concentration of a bicarbonate buffered physiological solution. Acclimation temperature did not modify the response to a rapid change in pH, but test temperature did. Following a pH decrease from 9.0 to 6.0, tetanic tension decreased at a faster rate at 5 degrees C than at 25 degrees C. A new steady state was reached in 15 min at 5 degrees C but in 40 min at 25 degrees C. Following a pH increase from 6.0 to 8.5, tetanic tension increased at a faster rate at 25 degrees C than at 5 degrees C. A new steady state was reached in 60 min at 5 degrees C but in 10 min at 25 degrees C. We conclude that the rate of carbon dioxide diffusion through the sartorius muscle is only one factor that determines how rapidly tetanic tension changes following the step change in pH, and that muscle resists pH change more effectively at higher temperatures.

Animals↗

PubMed1984

Chemical energy usage during isometric twitches of frog sartorius muscle intoxicated with an isomer of creatine, beta-guanidinopropionate.

Frogs were injected for several weeks with beta-guanidinopropionate, an isomer of creatine. Their sartorius muscles were isolated, poisoned with iodoacetate and stimulated isometrically with 75 shocks/min in nitrogen until rigor. In comparison with sartorius muscles of untreated frogs, they contained more free creatine and less phosphocreatine, but the same content in total creatine and ATP. They also contained beta-guanidinopropionate both free and phosphorylated. However, muscles in rigor contained the same concentration of the phosphorylated form as resting muscles, i.e., phospho-beta-guanidinopropionate was not split during contraction. The number of twitches performed before rigor was decreased. There was no change in the chemical energy usage (sum of phosphocreatine breakdown and twice ATP breakdown) per twitch.

Adenosine Triphosphate↗

PubMed1985

Development of the mature distribution of synapses on fibres in the frog sartorius muscle.

Most of the fibres in mature frog sartorius muscle possess two or more synapses separated by up to one-third the length of the muscle. The aim of the present work was to determine how the relative distances between these synapses changes during development in the frog (Limnodynastes tasmaniansis), as the fibres increase in length from 2 mm (stage 56) to 20 mm (1 year postmetamorphosis). At the earliest stage investigated (fibres 2.0-4.0 mm in length; stages 56-57) about 80% of the fibres were innervated at two endplates. The percentage of fibres with two endplates then remained approximately constant with further development. The polyneuronal innervation of endplates was almost eliminated by stage 57. Muscle fibres with two endplates had each situated on average about one-third the length of the fibre from a tendinous insertion; these relative positions did not change throughout development. Thus the distance between endplates increased linearly with an increase in fibre length. The size of terminals and the complexity of their branching also increased continually throughout development, independently of the location of the terminals on the fibres. The observations suggest that the distance between terminals increases during development because of the intercalation of new plasma membrane and basal lamina associated with the increase in length and diameter of fibres.

Aging↗

PubMed1986

Influence of external barium and potassium on potassium efflux in depolarized frog sartorius muscles.

Efflux of 42K+ was measured in frog sartorius muscles equilibrated in depolarizing solutions with external K+ concentrations [( K+]0) between 75 and 300 mM and NaCl concentrations of 60, 120, or 240 mM. For several combinations of KCl and NaCl, steady-state internal potentials (Vi) were the same for different [K+]0. For the range of Vi examined, K+ efflux occurs principally through the K+ inward rectifier channels. When external K+ is removed Vi remains constant for 2 to 3 hr because of the high membrane conductance to Cl-, but K+ efflux drops by about one order of magnitude. External Ba2+ in the presence or absence of external K+ produces an inhibition of K+ efflux described by a relation of the form u = (u1/(1 + C [Ba2+]0] + u2, where u is the uninhibited fraction of K+ efflux; u1, u2 and C are constants; and u1 + u2 = 1. C depends both on [K+]0 and Vi. When [K+]0 greater than or equal to 75 mM, increasing [K+]0 at constant Vi reduces Ba2+ sensitivity. For constant Vi greater than or equal to -30 mV, Ba2+ sensitivity is less when [K+]0 = 0 than when [K+]0 greater than or equal to 75 mM. When [K+]0 = 0, Ba2+ sensitivity decreases as Vi is made more positive. The dependence of the Ba2+ sensitivity on Vi at constant [K+]0 is greater when [K+]0 = 0 than when [K+]0 greater than or equal to 75 mM. Both the activation of K+ efflux by external K+ and the Ba2+ inhibition of K+ efflux can be explained on the basis of two membrane control sites associated with each channel. When both sites are occupied by K+, the channels are in a high flux state. When one or both sites are empty, the channels are in a low, nonzero flux state. When Ba2+ occupies either site, K+ efflux is further reduced. The reduction of Ba2+-sensitivity by increasing [K+]0 at high [K+]0 is attributable to the displacement of Ba2+ from the control sites by K+. The increased Ba2+ sensitivity produced by going from [K+]0 = 0 to [K+] greater than or equal to 75 mM when Vi greater than or equal to -30 mV is attributable to states in which Ba2+ occupies one site and K+ the other when [K+]0 not equal to 0.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗

PubMed1976

Effect of occlusion duration on reactive hyperemia in sartorius muscle capillaries.

Reactive hyperemia was studied in cat sartorius muscle by measurement of venous outflow and capillary red cell velocity following occlusions of 5-120 s. The peak value for volume flow rose in a graded manner as occlusion duration increased, reaching a level of 280% above control following 120 s of occlusion. By contrast, peak values for capillary flow were 200% above control even after short (10-15 s) occlusions and increased moderately thereafter to 280% above control following 120-s occlusions.

Animals↗

PubMed1989

The effect of lactate on intracellular pH and force recovery of fatigued sartorius muscles of the frog, Rana pipiens.

1. The effects of pHo (extracellular pH) and lactic acid on pHi (intracellular pH) and tetanic force were examined in frog sartorius muscle. Ion-selective microelectrodes were used to measure pHi. Tetanic force was elicited by field stimulation. Experiments were performed in HEPES-buffered solution equilibrated with 100% O2. 2. Mean pHi values (+/- S.E.M.) of unfatigued frog sartorius muscles were 7.14 +/- 0.02 and 7.05 +/- 0.09 at pHo 7.2 and 6.4, respectively. 3. A stimulation at a rate of one 100 ms tetanic contraction per second for 3 min reduced pHi to 6.21 +/- 0.09 and 6.20 +/- 0.04 at pHo 7.2 and 6.4, respectively. Meanwhile at pHo 7.2, the tetanic force (defined as the maximum force developed during a tetanus) decreased by 82.9 +/- 2.6%, the maximum rate of relaxation decreased by 92.9 +/- 0.9%, and the rate constant of the relaxation decreased by 88.5 +/- 1.6%. At pHo 6.4, the decrease in tetanic force, maximum rate of relaxation and rate constant were 90.6 +/- 1.8%, 93.8 +/- 0.5 and 87.5 +/- 2.7%, respectively. 4. The maximum rates of recovery of pHi following fatigue were 0.068 +/- 0.05 and 0.025 +/- 0.05 pH units min-1 at pHo 7.2 and 6.4, respectively. Recovery of normal tetanic force and relaxation rate was also slower at acidic pHo than at neutral pHo. 5. In the presence of 40 mmol l-1 L-lactic acid at pHo 7.2, the maximum rate of pHi recovery following fatigue was only 0.027 +/- 0.03 pH units min-1 at pHo 7.2. The presence of lactic acid also reduced the recovery of the relaxation phase, but not the recovery of tetanic force. 6. It is suggested that pHi recovery is not a limiting factor for tetanic force recovery and that the extracellular H+ inhibits tetanic force recovery by acting at a site located on the outer surface of the sarcolemma. The recovery of the relaxation phase is believed to be pHi dependent.

Animals↗

PubMed1978

Tension responses of frog sartorius muscle to quick ramp-shaped shortenings and some effects of metabolic inhibition.

The dynamic properties of the contractile mechanism of skeletal muscle were investigated in electrically stimulated sartorius muscle of the frog at 0 degrees C by analysis of the force responses to quick changes in length. 1. The tension responses to ramp-shaped shortenings at different rates of shortening have been recorded. The tension course during the ramp is described in terms of a damped elastic element. In addition a maximum value for an additional series elastic element is estimated. 2. The tension reponses to pulse-shaped shortenings have been recorded. At least 15 ms after the pulse a minimum in tension is found, which depends on amplitude and duration of the pulse. This effect is optimal at a pulse duration of about 6 ms. It is concluded that within 15 ms after the pulse detachment of crossbridges occurs. 3. Muscles were incubated with the metabolic inhibitors IAA and FAA or IAA and FDNB. After repeated stimulation, but before the muscle passes into the rigor state, the fast recovery and the plateau in the responses to quick shortenings are either diminished or abolished, depending on the amplitude of the shortening. This can be explained by assuming that in the unpoisened muscle, ATP enables the crossbridges to exert temporarily a larger force.

Adenosine Triphosphate↗